Engineering of interstitial foreign chromosome segments containing the K+/Na+ selectivity gene Kna1 by sequential homoeologous recombination in durum wheat View Full Text


Ontology type: schema:ScholarlyArticle      Open Access: True


Article Info

DATE

1996-11

AUTHORS

M. C. Luo, J. Dubcovsky, S. Goyal, J. Dvořák

ABSTRACT

Targeted homoeologous recombination mediated by the absence of the Ph1 locus is currently the most efficient technique by which foreign genes can be introgressed into polyploid wheat species. Because intra-arm homoeologous double cross-overs are rare, introgressed foreign genes are usually on terminal foreign chromosome segments. Since the minimum length of such a segment is determined by the position of a gene in the chromosome, large chromosome segments with undesirable genetic effects are often introgressed. Introgression of foreign genes on short interstitial segments based on two cycles of homoeologous recombination is described here. The utility of the technique is demonstrated by the introgression of the Kna1 locus, which controls K(+)/Na(+) selectivity in T. aesivum L., on short interstitial segments of chromosome 4D into chromosome 4B of Triticum turgidum L. The level of recombination in a homoeologous segment is not significantly affected by a juxtaposed proximal homologous segment in the absence of the Ph1 locus. More... »

PAGES

1180-1184

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf00230144

DOI

http://dx.doi.org/10.1007/bf00230144

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1001855412

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/24162500


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