Comprehensive molecular characterization of tissue-culture-derived Hordeum marinum plants View Full Text


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Article Info

DATE

1991-11

AUTHORS

D. Shimron-Abarbanell, A. Breiman

ABSTRACT

Scuttelar calli of Hordeum marinum readily and efficiently regenerate functional plants. In order to assess genetic variability among the regenerants we employed multiple analytic tools, which included molecular and biochemical assays. Total DNA extract from regenerated plants was digested with at least two restriction enzymes and hybridized to four nuclear and six mitochondrial coding sequences, in addition to one nuclear and three mitochondrial noncoding probes. SDS-PAGE analyses of hordein extracted from seeds of regenerated plants and activity assays of α-amylase were also performed. The nuclear and mitochondrial genomes of 50 regenerated plants demonstrated relative stability when assessed with coding sequences and by biochemical analyses. However, the mitochondrial noncoding probes revealed one qualitative somaclonal variant characterized by a loss of a hybridizing fragment. Moreover, changes in the methylation patterns of the rRNA genes and the nontranscribed spacer were revealed in another regenerated plant. The albino plant regenerated was characterized by a loss of three chloroplast DNA BamHI fragments. More... »

PAGES

71-80

References to SciGraph publications

  • 1984-01. Uniformity of plants regenerated from somatic embryos of Panicum maximum Jacq. (Guinea grass) in THEORETICAL AND APPLIED GENETICS
  • 1985-01. Deleted forms of plastid DNA in albino plants from cereal anther culture in CURRENT GENETICS
  • 1983-11. Somaclonal genetics of rice, Oryza sativa L. in THEORETICAL AND APPLIED GENETICS
  • 1987-05. Gametoclonal variation detected in the nuclear ribosomal DNA from doubled haploid lines of a spring wheat (Triticum aestivum L., cv. ‘César’) in THEORETICAL AND APPLIED GENETICS
  • 1983-05. Gibberellic-acid-regulated expression of α-amylase and six other genes in wheat aleurone layers in PLANTA
  • 1987-05. Heritable somaclonal variation in wild barley (Hordeum spontaneum) in THEORETICAL AND APPLIED GENETICS
  • 1982-12. Chromosome and nucleotide sequence differentiation in genomes of polyploid Triticum species in THEORETICAL AND APPLIED GENETICS
  • 1989-03. Plant regeneration from immature and mature embryo derived calli of Hordeum marinum in PLANT CELL, TISSUE AND ORGAN CULTURE (PCTOC)
  • 1988-10. Evidence for a direct relationship between mitochondrial genome organization and regeneration ability in hexaploid wheat somatic tissue cultures in CURRENT GENETICS
  • 1987-09. Extensive mitochondrial DNA variation in somatic tissue cultures initiated from wheat immature embryos in CURRENT GENETICS
  • 1987-02. Changes in methylation of tissue cultured soybean cells detected by digestion with the restriction enzymes HpaII and MspI in PLANT CELL REPORTS
  • 1984-03. Heritable somaclonal variation in wheat in THEORETICAL AND APPLIED GENETICS
  • 1987-04. Nor loci analysis in progenies of plants regenerated from the scutellar callus of bread-wheat in THEORETICAL AND APPLIED GENETICS
  • 1989-01. Rearrangement, amplification, and assortment of mitochondrial DNA molecules in cultured cells of Brassica campestris in THEORETICAL AND APPLIED GENETICS
  • 1985-03. Cytogenetic characterization of embryogenic callus and regenerated plants of Pennisetum americanum (L.) K. Schum in THEORETICAL AND APPLIED GENETICS
  • Journal

    TITLE

    Theoretical and Applied Genetics

    ISSUE

    1

    VOLUME

    83

    Author Affiliations

    Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/bf00229228

    DOI

    http://dx.doi.org/10.1007/bf00229228

    DIMENSIONS

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    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/24202259


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    40 schema:description Scuttelar calli of Hordeum marinum readily and efficiently regenerate functional plants. In order to assess genetic variability among the regenerants we employed multiple analytic tools, which included molecular and biochemical assays. Total DNA extract from regenerated plants was digested with at least two restriction enzymes and hybridized to four nuclear and six mitochondrial coding sequences, in addition to one nuclear and three mitochondrial noncoding probes. SDS-PAGE analyses of hordein extracted from seeds of regenerated plants and activity assays of α-amylase were also performed. The nuclear and mitochondrial genomes of 50 regenerated plants demonstrated relative stability when assessed with coding sequences and by biochemical analyses. However, the mitochondrial noncoding probes revealed one qualitative somaclonal variant characterized by a loss of a hybridizing fragment. Moreover, changes in the methylation patterns of the rRNA genes and the nontranscribed spacer were revealed in another regenerated plant. The albino plant regenerated was characterized by a loss of three chloroplast DNA BamHI fragments.
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