Synthesis of hexaploid (AABBCC) somatic hybrids: a bridging material for transfer of ‘tour’ cytoplasmic male sterility to different Brassica species View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1996-05

AUTHORS

N. Arumugam, A. Mukhopadhyay, V. Gupta, D. Pental, A. K. Pradhan

ABSTRACT

Most of the alloplasmic cytoplasmic male sterility (CMS) systems are known to be associated with a number of floral abnormalities that result from nuclear-cytoplasmic incompatibilities. One such system, 'tour', which is derived from Brassica tournefortii, induces additional floral abnormalities and causes chlorosis in Brassica spp. While the restorer for this CMS has been reported to be present in B. napus, in B. juncea, where the abnormalities are more pronounced, no restorer has yet been identified. Rectification of these floral abnormalities through mitochondrial recombinations and chloroplast replacement might result in the improvement of this CMS system. As organelle recombinations can possibly be achieved only by somatic cell hybridization, fusion experiments were carried out between hygromycin-resistant B. juncea AABB carrying 'tour' cytoplasm and phosphinotricin-resistant, normal B. oleracea CC to generate AABBCC hexaploid somatic hybrids. The presence of selectable marker genes facilitated the selection of hybrids in large numbers. The resulting hybrids showed wide variation in floral morphology and organelle composition. Regenerants with normal, male-sterile flowers having recombinant 'tour'-or 'oleracea'-type mitochondria and 'oleracea'-type chloroplasts were obtained. Hybrids with male-fertile flowers were also obtained that had recombined 'tour' mitochondria. The AABBCC hexaploid hybrids synthesized in the present study were successfully utilized as a bridging material for transferring variability in the organelle genome simultaneously to all the digenomic Brassica species, and all of these hybrids are now being stabilized through repeated backcrosses to the allopolyploid crop brassicas. More... »

PAGES

762-768

References to SciGraph publications

  • 1990-02. Male sterility caused by cytoplasm of Brassica oxyrrhina in B. campestris and B. juncea in THEORETICAL AND APPLIED GENETICS
  • 1994-09. Variation among offspring of alloplasmic tobacco Nicotiana tabacum L. cv ‘Zamojska 4’ with the cytoplasm of N. knightiana Goodspeed in THEORETICAL AND APPLIED GENETICS
  • 1988-01. Use of protoplasts in plant breeding: Cytoplasmic aspects in PLANT CELL, TISSUE AND ORGAN CULTURE (PCTOC)
  • 1970-10. Cytoplasmic male sterility in THE BOTANICAL REVIEW
  • 1994-09. Somatic hybrids with substitution type genomic configuration TCBB for the transfer of nuclear and organelle genes from Brassica tournefortii TT to allotetraploid oilseed crop B. carinata BBCC in THEORETICAL AND APPLIED GENETICS
  • 1989-11. Production of Cytoplasmic Male Sterile Rice (Oryza Sativa L.) by Cell Fusion in NATURE BIOTECHNOLOGY
  • 1991-03. Cytoplasmic male sterility in sunflower: comparison of molecular biology and genetic studies in PLANT MOLECULAR BIOLOGY
  • 1993-05. Variation amongst Brassica juncea cultivars for regeneration from hypocotyl explants and optimization of conditions for Agrobacterium-mediated genetic transformation in PLANT CELL REPORTS
  • 1991-10. Efficient regeneration of Brassica oleracea hypocotyl protoplasts and high frequency genetic transformation by direct DNA uptake in PLANT CELL REPORTS
  • 1992-11. Phytogeny of Brassica and allied genera based on variation in chloroplast and mitochondrial DNA patterns: molecular and taxonomic classifications are incongruous in THEORETICAL AND APPLIED GENETICS
  • 1983-08. Intergeneric cytoplasmic hybridization in cruciferae by protoplast fusion in MOLECULAR GENETICS AND GENOMICS
  • 1983-09. A plant DNA minipreparation: Version II in PLANT MOLECULAR BIOLOGY REPORTER
  • Journal

    TITLE

    Theoretical and Applied Genetics

    ISSUE

    6

    VOLUME

    92

    Author Affiliations

    Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/bf00226099

    DOI

    http://dx.doi.org/10.1007/bf00226099

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1010839995

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/24166401


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    31 schema:description Most of the alloplasmic cytoplasmic male sterility (CMS) systems are known to be associated with a number of floral abnormalities that result from nuclear-cytoplasmic incompatibilities. One such system, 'tour', which is derived from Brassica tournefortii, induces additional floral abnormalities and causes chlorosis in Brassica spp. While the restorer for this CMS has been reported to be present in B. napus, in B. juncea, where the abnormalities are more pronounced, no restorer has yet been identified. Rectification of these floral abnormalities through mitochondrial recombinations and chloroplast replacement might result in the improvement of this CMS system. As organelle recombinations can possibly be achieved only by somatic cell hybridization, fusion experiments were carried out between hygromycin-resistant B. juncea AABB carrying 'tour' cytoplasm and phosphinotricin-resistant, normal B. oleracea CC to generate AABBCC hexaploid somatic hybrids. The presence of selectable marker genes facilitated the selection of hybrids in large numbers. The resulting hybrids showed wide variation in floral morphology and organelle composition. Regenerants with normal, male-sterile flowers having recombinant 'tour'-or 'oleracea'-type mitochondria and 'oleracea'-type chloroplasts were obtained. Hybrids with male-fertile flowers were also obtained that had recombined 'tour' mitochondria. The AABBCC hexaploid hybrids synthesized in the present study were successfully utilized as a bridging material for transferring variability in the organelle genome simultaneously to all the digenomic Brassica species, and all of these hybrids are now being stabilized through repeated backcrosses to the allopolyploid crop brassicas.
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