Organellar segregation, rearrangement and recombination in protoplast fusion-derived Brassica oleracea calli View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1993-02

AUTHORS

T. W. Walters, E. D. Earle

ABSTRACT

Cauliflower protoplasts were fused to determine the effect of protoplast source and pretreatment on organellar segregation in fusion products. Mitochondrial and chloroplast type were determined for over 250 calli from eight fusions between iodoacetate-treated or γ-irradiated leaf or hypocotyl protoplasts with fertile or Ogura cytoplasms. Organelles in fusion-derived calli were identified with five mitochondrial probes and one chloroplast probe. Mitochondrial and chloroplast segregation were independent but biased. Most calli had B. oleracea chloroplasts, but more calli had Ogura mitochondria than B. oleracea ones. Neither protoplast source nor pretreatment alone affected organelle segregation. However, iodoacetate treatment of hypocotyl protoplasts reduced their mitochondrial contribution to the fusion products although it did not affect chloroplast segregation. Over half of the calli had mitochondrial genomes distinct from those of either fusion partner; many of these contained the complete mitochondrial genome of one partner along with some mitochondrial DNA from the other. Out of 258 calli, 83 showed evidence of mitochondrial recombination, most commonly by formation of a novel 11-kb PstI fragment near the atp9 region. More... »

PAGES

761-769

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  • Journal

    TITLE

    Theoretical and Applied Genetics

    ISSUE

    6-7

    VOLUME

    85

    Author Affiliations

    Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/bf00225017

    DOI

    http://dx.doi.org/10.1007/bf00225017

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1044708433

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/24196048


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