Selection of transformed protoplast-derived Zea mays colonies with phosphinothricin and a novel assay using the pH indicator chlorophenol red View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1993-06

AUTHORS

Catherine Kramer, Joe DiMaio, Gleta K. Carswell, Raymond D. Shillito

ABSTRACT

Phosphinothricin (PPT) inhibits glutamine synthetase in plant cells, resulting in an accumulation of ammonia that can be toxic. Bar, from Streptomyces hygroscopicus is a gene that codes for phosphinothricin acetyl-transferase, an enzyme that detoxifies PPT by acetylation. We used polyethylene-glycol-mediated transformation to introduce bar into protoplasts of maize (Zea mays L.). With a novel assay utilizing the pH indicator chlorophenol red we identified transformants after only two to four weeks of selection on PPT. More... »

PAGES

454-458

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf00224783

DOI

http://dx.doi.org/10.1007/bf00224783

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1053107303


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