Species-specific sequences in the genus Solanum: identification, characterization, and application to study somatic hybrids of S. brevidens and S. tuberosum View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1990-11

AUTHORS

E. Pehu, M. Thomas, T. Poutala, A. Karp, M. G. K. Jones

ABSTRACT

To aid in the identification and analysis of somatic hybrids between potato (Solanum tuberosum, dihaploid line PDH 40) and the non tuber-bearing wild species S. brevidens, a series of species-specific repetitive DNA sequences have been isolated. This was accomplished by making libraries of HaeIII-digested total DNA of S. tuberosum and S. brevidens, by cloning fragments into the SmaI site of plasmid pUC18 and transforming them into E. coli (JM83). The S. brevidens library consisted of 1,000 recombinant clones, and that of S. tuberosum, 700. Nitrocellulose filters with recombinant clones were hybridised to nick-translated total DNA of S. brevidens and also S. tuberosum, and, following autoradiography, clones that hybridised strongly to the DNA of only one of the species were chosen. Two highly repeated S. brevidens clones (pSB1, 400 bp and pSB7,210 bp), one highcopy-number s. tuberosum clone (pST10, 200 bp) and one low-copy-number sequence of S. tuberosum (pST3, 1.5 kbp) were selected for further analysis by Southern hybridisation to digested total DNA. Clone pSB7 gave a ladder pattern on hybridisation to EcoR1-digested total DNA of S. brevidens, with signals at multiples of 200 bp DNA. Using these probes it was possible to verify the hybridity of putative hybrids of dihaploid S. tuberosum and S.brevidens, and to confirm by Southern analysis and by slot blots the parental genome dosage of hexaploid hybrids (two s. brevidens: one S. tuberosum, and vice-versa). The S. tuberosum-specific probe, pSTIO, hybridised with DNA of three other tuber-bearing wild species (S. hjertingii, S. capsicibaccatum and S. berthaultii). A squash-blot procedure was developed using the probes that would allow early identification of somatic hybrid callus. There are a number of useful applications of such species-specific probes in the identification and analysis of somatic hybrids. More... »

PAGES

693-698

References to SciGraph publications

  • 1984-12. Transmission genetics of the somatic hybridization process in Nicotiana in THEORETICAL AND APPLIED GENETICS
  • 1986-02. Somatic hybrid plants between Lycopersicon esculentum and Solanum lycopersicoides in THEORETICAL AND APPLIED GENETICS
  • 1986-02. Somatic hybrids produced by protoplast fusion between S. tuberosum and S. brevidens: phenotypic variation under field conditions in THEORETICAL AND APPLIED GENETICS
  • 1988-08. A molecular and cytogenetic survey of major repeated DNA sequences in tomato (Lycopersicon esculentum) in MOLECULAR GENETICS AND GENOMICS
  • 1988-08. Diploid Brassica napus somatic hybrids: characterization of nuclear and organellar DNA in THEORETICAL AND APPLIED GENETICS
  • 1989-05. Analysis of chloroplast and mitochondrial DNAs in asymmetric somatic hybrids between tobacco and carrot in THEORETICAL AND APPLIED GENETICS
  • 1986-03. Somatic hybridization of Nicotiana tabacum and Petunia hybrida in MOLECULAR GENETICS AND GENOMICS
  • 1989-07. Effect of repeated DNA sequences on direct gene transfer in protoplasts of Nicotiana plumbaginifolia in THEORETICAL AND APPLIED GENETICS
  • 1987-12. Interspecific hybrid plant formation by electrofusion in Nicotiana in THEORETICAL AND APPLIED GENETICS
  • 1983-09. A plant DNA minipreparation: Version II in PLANT MOLECULAR BIOLOGY REPORTER
  • 1988-11. Genome specific, highly repeated sequences of Hordeum vulgare: cloning, sequencing and squash dot test in THEORETICAL AND APPLIED GENETICS
  • 1986-01. Production and characterization of somatic hybrids between Solanum melongena L. and S. sisymbriifolium Lam. in THEORETICAL AND APPLIED GENETICS
  • 1987-08. Improved isolation of dihaploidsolanum tubersoum protoplasts and the production of somatic hybrids between dihaploidS. tuberosum andS. brevidens in IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY
  • 1985-04. Expression of nuclear-cytoplasmic genomic incompatibility in interspecific Petunia somatic hybrid plants in THEORETICAL AND APPLIED GENETICS
  • 1988-08. A selection method for the synthesis of triploid hybrids by fusion of microspore protoplasts (n) with somatic cell protoplasts (2n) in THEORETICAL AND APPLIED GENETICS
  • 1984-04. Species-specific repetitive DNA used to identify interspecific somatic hybrids in PLANT CELL REPORTS
  • 1988-08. Production of somatic hybrids by electrofusion in Solanum in THEORETICAL AND APPLIED GENETICS
  • 1989-05. Comparative and genetic studies of isozymes in resynthesized and cultivated Brassica napus L., B. campestris L. and B. alboglabra Bailey in THEORETICAL AND APPLIED GENETICS
  • 1986-03. Wheat specific repetitive DNA sequences — construction and characterization of four different genomic clones in THEORETICAL AND APPLIED GENETICS
  • 1987-12. Citrus cybrids: production by donor-recipient protoplast-fusion and verification by mitochondrial-DNA restriction profiles in THEORETICAL AND APPLIED GENETICS
  • 1989-11. Molecular, cytogenetic and morphological characterization of somatic hybrids of dihaploid Solanum tuberosum and diploid S. brevidens in THEORETICAL AND APPLIED GENETICS
  • 1988-09. In situ hybridization to somatic metaphase chromosomes of potato in THEORETICAL AND APPLIED GENETICS
  • Journal

    TITLE

    Theoretical and Applied Genetics

    ISSUE

    5

    VOLUME

    80

    Author Affiliations

    Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/bf00224231

    DOI

    http://dx.doi.org/10.1007/bf00224231

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1004139792

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/24221078


    Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
    Incoming Citations Browse incoming citations for this publication using opencitations.net

    JSON-LD is the canonical representation for SciGraph data.

    TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

    [
      {
        "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
        "about": [
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0604", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Genetics", 
            "type": "DefinedTerm"
          }, 
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Biological Sciences", 
            "type": "DefinedTerm"
          }
        ], 
        "author": [
          {
            "affiliation": {
              "alternateName": "Rothamsted Research", 
              "id": "https://www.grid.ac/institutes/grid.418374.d", 
              "name": [
                "Department of Biochemistry and Physiology, AFRC Institute of Arable Crops Research, Rothamsted Experimental Station, AL5 2JQ, Harpenden, Herts., UK"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Pehu", 
            "givenName": "E.", 
            "id": "sg:person.01235023666.54", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01235023666.54"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Rothamsted Research", 
              "id": "https://www.grid.ac/institutes/grid.418374.d", 
              "name": [
                "Department of Biochemistry and Physiology, AFRC Institute of Arable Crops Research, Rothamsted Experimental Station, AL5 2JQ, Harpenden, Herts., UK"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Thomas", 
            "givenName": "M.", 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Rothamsted Research", 
              "id": "https://www.grid.ac/institutes/grid.418374.d", 
              "name": [
                "Department of Biochemistry and Physiology, AFRC Institute of Arable Crops Research, Rothamsted Experimental Station, AL5 2JQ, Harpenden, Herts., UK"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Poutala", 
            "givenName": "T.", 
            "id": "sg:person.0646364020.60", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0646364020.60"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Rothamsted Research", 
              "id": "https://www.grid.ac/institutes/grid.418374.d", 
              "name": [
                "Department of Biochemistry and Physiology, AFRC Institute of Arable Crops Research, Rothamsted Experimental Station, AL5 2JQ, Harpenden, Herts., UK"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Karp", 
            "givenName": "A.", 
            "id": "sg:person.01163262030.73", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01163262030.73"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Rothamsted Research", 
              "id": "https://www.grid.ac/institutes/grid.418374.d", 
              "name": [
                "Department of Biochemistry and Physiology, AFRC Institute of Arable Crops Research, Rothamsted Experimental Station, AL5 2JQ, Harpenden, Herts., UK"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Jones", 
            "givenName": "M. G. K.", 
            "id": "sg:person.016427007024.24", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.016427007024.24"
            ], 
            "type": "Person"
          }
        ], 
        "citation": [
          {
            "id": "sg:pub.10.1007/bf00299763", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1000677365", 
              "https://doi.org/10.1007/bf00299763"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00299763", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1000677365", 
              "https://doi.org/10.1007/bf00299763"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0167-7799(88)90085-6", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1002992948"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf02712670", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1003131267", 
              "https://doi.org/10.1007/bf02712670"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf02712670", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1003131267", 
              "https://doi.org/10.1007/bf02712670"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00263265", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1003180120", 
              "https://doi.org/10.1007/bf00263265"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00263265", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1003180120", 
              "https://doi.org/10.1007/bf00263265"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00339590", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1004424855", 
              "https://doi.org/10.1007/bf00339590"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00339590", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1004424855", 
              "https://doi.org/10.1007/bf00339590"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00270973", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1005041642", 
              "https://doi.org/10.1007/bf00270973"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00270973", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1005041642", 
              "https://doi.org/10.1007/bf00270973"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00257854", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1006138863", 
              "https://doi.org/10.1007/bf00257854"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00257854", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1006138863", 
              "https://doi.org/10.1007/bf00257854"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/s0022-2836(77)80102-2", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1010792696"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0003-2697(83)90418-9", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1012973560"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00249142", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1013652536", 
              "https://doi.org/10.1007/bf00249142"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00249142", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1013652536", 
              "https://doi.org/10.1007/bf00249142"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00264265", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1019824553", 
              "https://doi.org/10.1007/bf00264265"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00264265", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1019824553", 
              "https://doi.org/10.1007/bf00264265"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00262566", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1019961829", 
              "https://doi.org/10.1007/bf00262566"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00262566", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1019961829", 
              "https://doi.org/10.1007/bf00262566"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1111/j.1399-3054.1962.tb08052.x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1023703429"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00257851", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1023839362", 
              "https://doi.org/10.1007/bf00257851"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00257851", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1023839362", 
              "https://doi.org/10.1007/bf00257851"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00303519", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1024143166", 
              "https://doi.org/10.1007/bf00303519"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00303519", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1024143166", 
              "https://doi.org/10.1007/bf00303519"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00261236", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1025682307", 
              "https://doi.org/10.1007/bf00261236"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00261236", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1025682307", 
              "https://doi.org/10.1007/bf00261236"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0168-9452(85)90195-5", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1030957608"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/s0022-2836(75)80083-0", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1033296365"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00266993", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1033829529", 
              "https://doi.org/10.1007/bf00266993"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00266993", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1033829529", 
              "https://doi.org/10.1007/bf00266993"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1073/pnas.81.7.1991", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1034589758"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00261242", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1037896514", 
              "https://doi.org/10.1007/bf00261242"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00261242", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1037896514", 
              "https://doi.org/10.1007/bf00261242"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1073/pnas.85.22.8568", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1038254146"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00265343", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1040126741", 
              "https://doi.org/10.1007/bf00265343"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00265343", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1040126741", 
              "https://doi.org/10.1007/bf00265343"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00264483", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1041387838", 
              "https://doi.org/10.1007/bf00264483"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00264483", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1041387838", 
              "https://doi.org/10.1007/bf00264483"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00333260", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1043286327", 
              "https://doi.org/10.1007/bf00333260"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00333260", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1043286327", 
              "https://doi.org/10.1007/bf00333260"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf02620975", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1049162310", 
              "https://doi.org/10.1007/bf02620975"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf02620975", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1049162310", 
              "https://doi.org/10.1007/bf02620975"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0378-1119(82)90015-4", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1050786151"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0378-1119(82)90015-4", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1050786151"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00249133", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1051020423", 
              "https://doi.org/10.1007/bf00249133"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00249133", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1051020423", 
              "https://doi.org/10.1007/bf00249133"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00263264", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1051305995", 
              "https://doi.org/10.1007/bf00263264"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00263264", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1051305995", 
              "https://doi.org/10.1007/bf00263264"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00257846", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1052308495", 
              "https://doi.org/10.1007/bf00257846"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00257846", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1052308495", 
              "https://doi.org/10.1007/bf00257846"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00272881", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1078815979", 
              "https://doi.org/10.1007/bf00272881"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00272881", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1078815979", 
              "https://doi.org/10.1007/bf00272881"
            ], 
            "type": "CreativeWork"
          }
        ], 
        "datePublished": "1990-11", 
        "datePublishedReg": "1990-11-01", 
        "description": "To aid in the identification and analysis of somatic hybrids between potato (Solanum tuberosum, dihaploid line PDH 40) and the non tuber-bearing wild species S. brevidens, a series of species-specific repetitive DNA sequences have been isolated. This was accomplished by making libraries of HaeIII-digested total DNA of S. tuberosum and S. brevidens, by cloning fragments into the SmaI site of plasmid pUC18 and transforming them into E. coli (JM83). The S. brevidens library consisted of 1,000 recombinant clones, and that of S. tuberosum, 700. Nitrocellulose filters with recombinant clones were hybridised to nick-translated total DNA of S. brevidens and also S. tuberosum, and, following autoradiography, clones that hybridised strongly to the DNA of only one of the species were chosen. Two highly repeated S. brevidens clones (pSB1, 400 bp and pSB7,210 bp), one highcopy-number s. tuberosum clone (pST10, 200 bp) and one low-copy-number sequence of S. tuberosum (pST3, 1.5 kbp) were selected for further analysis by Southern hybridisation to digested total DNA. Clone pSB7 gave a ladder pattern on hybridisation to EcoR1-digested total DNA of S. brevidens, with signals at multiples of 200 bp DNA. Using these probes it was possible to verify the hybridity of putative hybrids of dihaploid S. tuberosum and S.brevidens, and to confirm by Southern analysis and by slot blots the parental genome dosage of hexaploid hybrids (two s. brevidens: one S. tuberosum, and vice-versa). The S. tuberosum-specific probe, pSTIO, hybridised with DNA of three other tuber-bearing wild species (S. hjertingii, S. capsicibaccatum and S. berthaultii). A squash-blot procedure was developed using the probes that would allow early identification of somatic hybrid callus. There are a number of useful applications of such species-specific probes in the identification and analysis of somatic hybrids. ", 
        "genre": "research_article", 
        "id": "sg:pub.10.1007/bf00224231", 
        "inLanguage": [
          "en"
        ], 
        "isAccessibleForFree": false, 
        "isPartOf": [
          {
            "id": "sg:journal.1135804", 
            "issn": [
              "0040-5752", 
              "1432-2242"
            ], 
            "name": "Theoretical and Applied Genetics", 
            "type": "Periodical"
          }, 
          {
            "issueNumber": "5", 
            "type": "PublicationIssue"
          }, 
          {
            "type": "PublicationVolume", 
            "volumeNumber": "80"
          }
        ], 
        "name": "Species-specific sequences in the genus Solanum: identification, characterization, and application to study somatic hybrids of S. brevidens and S. tuberosum", 
        "pagination": "693-698", 
        "productId": [
          {
            "name": "doi", 
            "type": "PropertyValue", 
            "value": [
              "10.1007/bf00224231"
            ]
          }, 
          {
            "name": "readcube_id", 
            "type": "PropertyValue", 
            "value": [
              "13ba13e08267d53aa75359635441f29c1472adb84ddd569cb88f959938e899f4"
            ]
          }, 
          {
            "name": "dimensions_id", 
            "type": "PropertyValue", 
            "value": [
              "pub.1004139792"
            ]
          }, 
          {
            "name": "nlm_unique_id", 
            "type": "PropertyValue", 
            "value": [
              "0145600"
            ]
          }, 
          {
            "name": "pubmed_id", 
            "type": "PropertyValue", 
            "value": [
              "24221078"
            ]
          }
        ], 
        "sameAs": [
          "https://doi.org/10.1007/bf00224231", 
          "https://app.dimensions.ai/details/publication/pub.1004139792"
        ], 
        "sdDataset": "articles", 
        "sdDatePublished": "2019-04-15T09:02", 
        "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
        "sdPublisher": {
          "name": "Springer Nature - SN SciGraph project", 
          "type": "Organization"
        }, 
        "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000375_0000000375/records_91459_00000000.jsonl", 
        "type": "ScholarlyArticle", 
        "url": "http://link.springer.com/10.1007/BF00224231"
      }
    ]
     

    Download the RDF metadata as:  json-ld nt turtle xml License info

    HOW TO GET THIS DATA PROGRAMMATICALLY:

    JSON-LD is a popular format for linked data which is fully compatible with JSON.

    curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/bf00224231'

    N-Triples is a line-based linked data format ideal for batch operations.

    curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/bf00224231'

    Turtle is a human-readable linked data format.

    curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/bf00224231'

    RDF/XML is a standard XML format for linked data.

    curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/bf00224231'


     

    This table displays all metadata directly associated to this object as RDF triples.

    211 TRIPLES      21 PREDICATES      60 URIs      21 LITERALS      9 BLANK NODES

    Subject Predicate Object
    1 sg:pub.10.1007/bf00224231 schema:about anzsrc-for:06
    2 anzsrc-for:0604
    3 schema:author Na818fd0ff3e8441eb512b17058659113
    4 schema:citation sg:pub.10.1007/bf00249133
    5 sg:pub.10.1007/bf00249142
    6 sg:pub.10.1007/bf00257846
    7 sg:pub.10.1007/bf00257851
    8 sg:pub.10.1007/bf00257854
    9 sg:pub.10.1007/bf00261236
    10 sg:pub.10.1007/bf00261242
    11 sg:pub.10.1007/bf00262566
    12 sg:pub.10.1007/bf00263264
    13 sg:pub.10.1007/bf00263265
    14 sg:pub.10.1007/bf00264265
    15 sg:pub.10.1007/bf00264483
    16 sg:pub.10.1007/bf00265343
    17 sg:pub.10.1007/bf00266993
    18 sg:pub.10.1007/bf00270973
    19 sg:pub.10.1007/bf00272881
    20 sg:pub.10.1007/bf00299763
    21 sg:pub.10.1007/bf00303519
    22 sg:pub.10.1007/bf00333260
    23 sg:pub.10.1007/bf00339590
    24 sg:pub.10.1007/bf02620975
    25 sg:pub.10.1007/bf02712670
    26 https://doi.org/10.1016/0003-2697(83)90418-9
    27 https://doi.org/10.1016/0167-7799(88)90085-6
    28 https://doi.org/10.1016/0168-9452(85)90195-5
    29 https://doi.org/10.1016/0378-1119(82)90015-4
    30 https://doi.org/10.1016/s0022-2836(75)80083-0
    31 https://doi.org/10.1016/s0022-2836(77)80102-2
    32 https://doi.org/10.1073/pnas.81.7.1991
    33 https://doi.org/10.1073/pnas.85.22.8568
    34 https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
    35 schema:datePublished 1990-11
    36 schema:datePublishedReg 1990-11-01
    37 schema:description To aid in the identification and analysis of somatic hybrids between potato (Solanum tuberosum, dihaploid line PDH 40) and the non tuber-bearing wild species S. brevidens, a series of species-specific repetitive DNA sequences have been isolated. This was accomplished by making libraries of HaeIII-digested total DNA of S. tuberosum and S. brevidens, by cloning fragments into the SmaI site of plasmid pUC18 and transforming them into E. coli (JM83). The S. brevidens library consisted of 1,000 recombinant clones, and that of S. tuberosum, 700. Nitrocellulose filters with recombinant clones were hybridised to nick-translated total DNA of S. brevidens and also S. tuberosum, and, following autoradiography, clones that hybridised strongly to the DNA of only one of the species were chosen. Two highly repeated S. brevidens clones (pSB1, 400 bp and pSB7,210 bp), one highcopy-number s. tuberosum clone (pST10, 200 bp) and one low-copy-number sequence of S. tuberosum (pST3, 1.5 kbp) were selected for further analysis by Southern hybridisation to digested total DNA. Clone pSB7 gave a ladder pattern on hybridisation to EcoR1-digested total DNA of S. brevidens, with signals at multiples of 200 bp DNA. Using these probes it was possible to verify the hybridity of putative hybrids of dihaploid S. tuberosum and S.brevidens, and to confirm by Southern analysis and by slot blots the parental genome dosage of hexaploid hybrids (two s. brevidens: one S. tuberosum, and vice-versa). The S. tuberosum-specific probe, pSTIO, hybridised with DNA of three other tuber-bearing wild species (S. hjertingii, S. capsicibaccatum and S. berthaultii). A squash-blot procedure was developed using the probes that would allow early identification of somatic hybrid callus. There are a number of useful applications of such species-specific probes in the identification and analysis of somatic hybrids.
    38 schema:genre research_article
    39 schema:inLanguage en
    40 schema:isAccessibleForFree false
    41 schema:isPartOf N20c3474326d94823a304b812004b16be
    42 N4ba5cc89861d4fd593acd70c57e607bd
    43 sg:journal.1135804
    44 schema:name Species-specific sequences in the genus Solanum: identification, characterization, and application to study somatic hybrids of S. brevidens and S. tuberosum
    45 schema:pagination 693-698
    46 schema:productId N0d4727a9b8ca47d391521be8675513a1
    47 N2d4795777b1444e2ae3644861c7d96c6
    48 N527f8e9845d744ad9e726e93a652af84
    49 Nbfec77fa5cf54c1a9b8110155a00868b
    50 Ncfb034b47de6475d97a1e73c9f622430
    51 schema:sameAs https://app.dimensions.ai/details/publication/pub.1004139792
    52 https://doi.org/10.1007/bf00224231
    53 schema:sdDatePublished 2019-04-15T09:02
    54 schema:sdLicense https://scigraph.springernature.com/explorer/license/
    55 schema:sdPublisher N9395e7be3981425d9655bf3bb3ada7e5
    56 schema:url http://link.springer.com/10.1007/BF00224231
    57 sgo:license sg:explorer/license/
    58 sgo:sdDataset articles
    59 rdf:type schema:ScholarlyArticle
    60 N0d4727a9b8ca47d391521be8675513a1 schema:name pubmed_id
    61 schema:value 24221078
    62 rdf:type schema:PropertyValue
    63 N20c3474326d94823a304b812004b16be schema:issueNumber 5
    64 rdf:type schema:PublicationIssue
    65 N2d4795777b1444e2ae3644861c7d96c6 schema:name doi
    66 schema:value 10.1007/bf00224231
    67 rdf:type schema:PropertyValue
    68 N4ba5cc89861d4fd593acd70c57e607bd schema:volumeNumber 80
    69 rdf:type schema:PublicationVolume
    70 N4e66ce850efd4880be6c0fd891bd5f05 rdf:first N6e728fa1e14d47cd88df2ac99e1b4445
    71 rdf:rest Nc9fe3189ff0f47409a9e0b4bff58b752
    72 N527f8e9845d744ad9e726e93a652af84 schema:name nlm_unique_id
    73 schema:value 0145600
    74 rdf:type schema:PropertyValue
    75 N6e728fa1e14d47cd88df2ac99e1b4445 schema:affiliation https://www.grid.ac/institutes/grid.418374.d
    76 schema:familyName Thomas
    77 schema:givenName M.
    78 rdf:type schema:Person
    79 N6ffa424179ce40d48973461c53e2247e rdf:first sg:person.01163262030.73
    80 rdf:rest N7a108530ec7441f59f8168ab4a60f182
    81 N7a108530ec7441f59f8168ab4a60f182 rdf:first sg:person.016427007024.24
    82 rdf:rest rdf:nil
    83 N9395e7be3981425d9655bf3bb3ada7e5 schema:name Springer Nature - SN SciGraph project
    84 rdf:type schema:Organization
    85 Na818fd0ff3e8441eb512b17058659113 rdf:first sg:person.01235023666.54
    86 rdf:rest N4e66ce850efd4880be6c0fd891bd5f05
    87 Nbfec77fa5cf54c1a9b8110155a00868b schema:name readcube_id
    88 schema:value 13ba13e08267d53aa75359635441f29c1472adb84ddd569cb88f959938e899f4
    89 rdf:type schema:PropertyValue
    90 Nc9fe3189ff0f47409a9e0b4bff58b752 rdf:first sg:person.0646364020.60
    91 rdf:rest N6ffa424179ce40d48973461c53e2247e
    92 Ncfb034b47de6475d97a1e73c9f622430 schema:name dimensions_id
    93 schema:value pub.1004139792
    94 rdf:type schema:PropertyValue
    95 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
    96 schema:name Biological Sciences
    97 rdf:type schema:DefinedTerm
    98 anzsrc-for:0604 schema:inDefinedTermSet anzsrc-for:
    99 schema:name Genetics
    100 rdf:type schema:DefinedTerm
    101 sg:journal.1135804 schema:issn 0040-5752
    102 1432-2242
    103 schema:name Theoretical and Applied Genetics
    104 rdf:type schema:Periodical
    105 sg:person.01163262030.73 schema:affiliation https://www.grid.ac/institutes/grid.418374.d
    106 schema:familyName Karp
    107 schema:givenName A.
    108 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01163262030.73
    109 rdf:type schema:Person
    110 sg:person.01235023666.54 schema:affiliation https://www.grid.ac/institutes/grid.418374.d
    111 schema:familyName Pehu
    112 schema:givenName E.
    113 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01235023666.54
    114 rdf:type schema:Person
    115 sg:person.016427007024.24 schema:affiliation https://www.grid.ac/institutes/grid.418374.d
    116 schema:familyName Jones
    117 schema:givenName M. G. K.
    118 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.016427007024.24
    119 rdf:type schema:Person
    120 sg:person.0646364020.60 schema:affiliation https://www.grid.ac/institutes/grid.418374.d
    121 schema:familyName Poutala
    122 schema:givenName T.
    123 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0646364020.60
    124 rdf:type schema:Person
    125 sg:pub.10.1007/bf00249133 schema:sameAs https://app.dimensions.ai/details/publication/pub.1051020423
    126 https://doi.org/10.1007/bf00249133
    127 rdf:type schema:CreativeWork
    128 sg:pub.10.1007/bf00249142 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013652536
    129 https://doi.org/10.1007/bf00249142
    130 rdf:type schema:CreativeWork
    131 sg:pub.10.1007/bf00257846 schema:sameAs https://app.dimensions.ai/details/publication/pub.1052308495
    132 https://doi.org/10.1007/bf00257846
    133 rdf:type schema:CreativeWork
    134 sg:pub.10.1007/bf00257851 schema:sameAs https://app.dimensions.ai/details/publication/pub.1023839362
    135 https://doi.org/10.1007/bf00257851
    136 rdf:type schema:CreativeWork
    137 sg:pub.10.1007/bf00257854 schema:sameAs https://app.dimensions.ai/details/publication/pub.1006138863
    138 https://doi.org/10.1007/bf00257854
    139 rdf:type schema:CreativeWork
    140 sg:pub.10.1007/bf00261236 schema:sameAs https://app.dimensions.ai/details/publication/pub.1025682307
    141 https://doi.org/10.1007/bf00261236
    142 rdf:type schema:CreativeWork
    143 sg:pub.10.1007/bf00261242 schema:sameAs https://app.dimensions.ai/details/publication/pub.1037896514
    144 https://doi.org/10.1007/bf00261242
    145 rdf:type schema:CreativeWork
    146 sg:pub.10.1007/bf00262566 schema:sameAs https://app.dimensions.ai/details/publication/pub.1019961829
    147 https://doi.org/10.1007/bf00262566
    148 rdf:type schema:CreativeWork
    149 sg:pub.10.1007/bf00263264 schema:sameAs https://app.dimensions.ai/details/publication/pub.1051305995
    150 https://doi.org/10.1007/bf00263264
    151 rdf:type schema:CreativeWork
    152 sg:pub.10.1007/bf00263265 schema:sameAs https://app.dimensions.ai/details/publication/pub.1003180120
    153 https://doi.org/10.1007/bf00263265
    154 rdf:type schema:CreativeWork
    155 sg:pub.10.1007/bf00264265 schema:sameAs https://app.dimensions.ai/details/publication/pub.1019824553
    156 https://doi.org/10.1007/bf00264265
    157 rdf:type schema:CreativeWork
    158 sg:pub.10.1007/bf00264483 schema:sameAs https://app.dimensions.ai/details/publication/pub.1041387838
    159 https://doi.org/10.1007/bf00264483
    160 rdf:type schema:CreativeWork
    161 sg:pub.10.1007/bf00265343 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040126741
    162 https://doi.org/10.1007/bf00265343
    163 rdf:type schema:CreativeWork
    164 sg:pub.10.1007/bf00266993 schema:sameAs https://app.dimensions.ai/details/publication/pub.1033829529
    165 https://doi.org/10.1007/bf00266993
    166 rdf:type schema:CreativeWork
    167 sg:pub.10.1007/bf00270973 schema:sameAs https://app.dimensions.ai/details/publication/pub.1005041642
    168 https://doi.org/10.1007/bf00270973
    169 rdf:type schema:CreativeWork
    170 sg:pub.10.1007/bf00272881 schema:sameAs https://app.dimensions.ai/details/publication/pub.1078815979
    171 https://doi.org/10.1007/bf00272881
    172 rdf:type schema:CreativeWork
    173 sg:pub.10.1007/bf00299763 schema:sameAs https://app.dimensions.ai/details/publication/pub.1000677365
    174 https://doi.org/10.1007/bf00299763
    175 rdf:type schema:CreativeWork
    176 sg:pub.10.1007/bf00303519 schema:sameAs https://app.dimensions.ai/details/publication/pub.1024143166
    177 https://doi.org/10.1007/bf00303519
    178 rdf:type schema:CreativeWork
    179 sg:pub.10.1007/bf00333260 schema:sameAs https://app.dimensions.ai/details/publication/pub.1043286327
    180 https://doi.org/10.1007/bf00333260
    181 rdf:type schema:CreativeWork
    182 sg:pub.10.1007/bf00339590 schema:sameAs https://app.dimensions.ai/details/publication/pub.1004424855
    183 https://doi.org/10.1007/bf00339590
    184 rdf:type schema:CreativeWork
    185 sg:pub.10.1007/bf02620975 schema:sameAs https://app.dimensions.ai/details/publication/pub.1049162310
    186 https://doi.org/10.1007/bf02620975
    187 rdf:type schema:CreativeWork
    188 sg:pub.10.1007/bf02712670 schema:sameAs https://app.dimensions.ai/details/publication/pub.1003131267
    189 https://doi.org/10.1007/bf02712670
    190 rdf:type schema:CreativeWork
    191 https://doi.org/10.1016/0003-2697(83)90418-9 schema:sameAs https://app.dimensions.ai/details/publication/pub.1012973560
    192 rdf:type schema:CreativeWork
    193 https://doi.org/10.1016/0167-7799(88)90085-6 schema:sameAs https://app.dimensions.ai/details/publication/pub.1002992948
    194 rdf:type schema:CreativeWork
    195 https://doi.org/10.1016/0168-9452(85)90195-5 schema:sameAs https://app.dimensions.ai/details/publication/pub.1030957608
    196 rdf:type schema:CreativeWork
    197 https://doi.org/10.1016/0378-1119(82)90015-4 schema:sameAs https://app.dimensions.ai/details/publication/pub.1050786151
    198 rdf:type schema:CreativeWork
    199 https://doi.org/10.1016/s0022-2836(75)80083-0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1033296365
    200 rdf:type schema:CreativeWork
    201 https://doi.org/10.1016/s0022-2836(77)80102-2 schema:sameAs https://app.dimensions.ai/details/publication/pub.1010792696
    202 rdf:type schema:CreativeWork
    203 https://doi.org/10.1073/pnas.81.7.1991 schema:sameAs https://app.dimensions.ai/details/publication/pub.1034589758
    204 rdf:type schema:CreativeWork
    205 https://doi.org/10.1073/pnas.85.22.8568 schema:sameAs https://app.dimensions.ai/details/publication/pub.1038254146
    206 rdf:type schema:CreativeWork
    207 https://doi.org/10.1111/j.1399-3054.1962.tb08052.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1023703429
    208 rdf:type schema:CreativeWork
    209 https://www.grid.ac/institutes/grid.418374.d schema:alternateName Rothamsted Research
    210 schema:name Department of Biochemistry and Physiology, AFRC Institute of Arable Crops Research, Rothamsted Experimental Station, AL5 2JQ, Harpenden, Herts., UK
    211 rdf:type schema:Organization
     




    Preview window. Press ESC to close (or click here)


    ...