Species-specific sequences in the genus Solanum: identification, characterization, and application to study somatic hybrids of S. brevidens and S. tuberosum View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1990-11

AUTHORS

E. Pehu, M. Thomas, T. Poutala, A. Karp, M. G. K. Jones

ABSTRACT

To aid in the identification and analysis of somatic hybrids between potato (Solanum tuberosum, dihaploid line PDH 40) and the non tuber-bearing wild species S. brevidens, a series of species-specific repetitive DNA sequences have been isolated. This was accomplished by making libraries of HaeIII-digested total DNA of S. tuberosum and S. brevidens, by cloning fragments into the SmaI site of plasmid pUC18 and transforming them into E. coli (JM83). The S. brevidens library consisted of 1,000 recombinant clones, and that of S. tuberosum, 700. Nitrocellulose filters with recombinant clones were hybridised to nick-translated total DNA of S. brevidens and also S. tuberosum, and, following autoradiography, clones that hybridised strongly to the DNA of only one of the species were chosen. Two highly repeated S. brevidens clones (pSB1, 400 bp and pSB7,210 bp), one highcopy-number s. tuberosum clone (pST10, 200 bp) and one low-copy-number sequence of S. tuberosum (pST3, 1.5 kbp) were selected for further analysis by Southern hybridisation to digested total DNA. Clone pSB7 gave a ladder pattern on hybridisation to EcoR1-digested total DNA of S. brevidens, with signals at multiples of 200 bp DNA. Using these probes it was possible to verify the hybridity of putative hybrids of dihaploid S. tuberosum and S.brevidens, and to confirm by Southern analysis and by slot blots the parental genome dosage of hexaploid hybrids (two s. brevidens: one S. tuberosum, and vice-versa). The S. tuberosum-specific probe, pSTIO, hybridised with DNA of three other tuber-bearing wild species (S. hjertingii, S. capsicibaccatum and S. berthaultii). A squash-blot procedure was developed using the probes that would allow early identification of somatic hybrid callus. There are a number of useful applications of such species-specific probes in the identification and analysis of somatic hybrids. More... »

PAGES

693-698

Journal

TITLE

Theoretical and Applied Genetics

ISSUE

5

VOLUME

80

Author Affiliations

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf00224231

DOI

http://dx.doi.org/10.1007/bf00224231

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1004139792

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/24221078


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0604", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Genetics", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Rothamsted Research", 
          "id": "https://www.grid.ac/institutes/grid.418374.d", 
          "name": [
            "Department of Biochemistry and Physiology, AFRC Institute of Arable Crops Research, Rothamsted Experimental Station, AL5 2JQ, Harpenden, Herts., UK"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Pehu", 
        "givenName": "E.", 
        "id": "sg:person.01235023666.54", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01235023666.54"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Rothamsted Research", 
          "id": "https://www.grid.ac/institutes/grid.418374.d", 
          "name": [
            "Department of Biochemistry and Physiology, AFRC Institute of Arable Crops Research, Rothamsted Experimental Station, AL5 2JQ, Harpenden, Herts., UK"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Thomas", 
        "givenName": "M.", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Rothamsted Research", 
          "id": "https://www.grid.ac/institutes/grid.418374.d", 
          "name": [
            "Department of Biochemistry and Physiology, AFRC Institute of Arable Crops Research, Rothamsted Experimental Station, AL5 2JQ, Harpenden, Herts., UK"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Poutala", 
        "givenName": "T.", 
        "id": "sg:person.0646364020.60", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0646364020.60"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Rothamsted Research", 
          "id": "https://www.grid.ac/institutes/grid.418374.d", 
          "name": [
            "Department of Biochemistry and Physiology, AFRC Institute of Arable Crops Research, Rothamsted Experimental Station, AL5 2JQ, Harpenden, Herts., UK"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Karp", 
        "givenName": "A.", 
        "id": "sg:person.01163262030.73", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01163262030.73"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Rothamsted Research", 
          "id": "https://www.grid.ac/institutes/grid.418374.d", 
          "name": [
            "Department of Biochemistry and Physiology, AFRC Institute of Arable Crops Research, Rothamsted Experimental Station, AL5 2JQ, Harpenden, Herts., UK"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Jones", 
        "givenName": "M. G. K.", 
        "id": "sg:person.016427007024.24", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.016427007024.24"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "sg:pub.10.1007/bf00299763", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1000677365", 
          "https://doi.org/10.1007/bf00299763"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00299763", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1000677365", 
          "https://doi.org/10.1007/bf00299763"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0167-7799(88)90085-6", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1002992948"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf02712670", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1003131267", 
          "https://doi.org/10.1007/bf02712670"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf02712670", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1003131267", 
          "https://doi.org/10.1007/bf02712670"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00263265", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1003180120", 
          "https://doi.org/10.1007/bf00263265"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00263265", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1003180120", 
          "https://doi.org/10.1007/bf00263265"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00339590", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1004424855", 
          "https://doi.org/10.1007/bf00339590"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00339590", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1004424855", 
          "https://doi.org/10.1007/bf00339590"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00270973", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1005041642", 
          "https://doi.org/10.1007/bf00270973"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00270973", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1005041642", 
          "https://doi.org/10.1007/bf00270973"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00257854", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1006138863", 
          "https://doi.org/10.1007/bf00257854"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00257854", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1006138863", 
          "https://doi.org/10.1007/bf00257854"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0022-2836(77)80102-2", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1010792696"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0003-2697(83)90418-9", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1012973560"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00249142", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1013652536", 
          "https://doi.org/10.1007/bf00249142"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00249142", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1013652536", 
          "https://doi.org/10.1007/bf00249142"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00264265", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1019824553", 
          "https://doi.org/10.1007/bf00264265"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00264265", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1019824553", 
          "https://doi.org/10.1007/bf00264265"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00262566", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1019961829", 
          "https://doi.org/10.1007/bf00262566"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00262566", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1019961829", 
          "https://doi.org/10.1007/bf00262566"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1399-3054.1962.tb08052.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1023703429"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00257851", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1023839362", 
          "https://doi.org/10.1007/bf00257851"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00257851", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1023839362", 
          "https://doi.org/10.1007/bf00257851"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00303519", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1024143166", 
          "https://doi.org/10.1007/bf00303519"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00303519", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1024143166", 
          "https://doi.org/10.1007/bf00303519"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00261236", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1025682307", 
          "https://doi.org/10.1007/bf00261236"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00261236", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1025682307", 
          "https://doi.org/10.1007/bf00261236"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0168-9452(85)90195-5", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1030957608"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0022-2836(75)80083-0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1033296365"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00266993", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1033829529", 
          "https://doi.org/10.1007/bf00266993"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00266993", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1033829529", 
          "https://doi.org/10.1007/bf00266993"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.81.7.1991", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1034589758"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00261242", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1037896514", 
          "https://doi.org/10.1007/bf00261242"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00261242", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1037896514", 
          "https://doi.org/10.1007/bf00261242"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.85.22.8568", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1038254146"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00265343", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1040126741", 
          "https://doi.org/10.1007/bf00265343"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00265343", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1040126741", 
          "https://doi.org/10.1007/bf00265343"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00264483", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1041387838", 
          "https://doi.org/10.1007/bf00264483"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00264483", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1041387838", 
          "https://doi.org/10.1007/bf00264483"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00333260", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1043286327", 
          "https://doi.org/10.1007/bf00333260"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00333260", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1043286327", 
          "https://doi.org/10.1007/bf00333260"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf02620975", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1049162310", 
          "https://doi.org/10.1007/bf02620975"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf02620975", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1049162310", 
          "https://doi.org/10.1007/bf02620975"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0378-1119(82)90015-4", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1050786151"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0378-1119(82)90015-4", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1050786151"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00249133", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1051020423", 
          "https://doi.org/10.1007/bf00249133"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00249133", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1051020423", 
          "https://doi.org/10.1007/bf00249133"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00263264", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1051305995", 
          "https://doi.org/10.1007/bf00263264"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00263264", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1051305995", 
          "https://doi.org/10.1007/bf00263264"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00257846", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1052308495", 
          "https://doi.org/10.1007/bf00257846"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00257846", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1052308495", 
          "https://doi.org/10.1007/bf00257846"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00272881", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1078815979", 
          "https://doi.org/10.1007/bf00272881"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00272881", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1078815979", 
          "https://doi.org/10.1007/bf00272881"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "1990-11", 
    "datePublishedReg": "1990-11-01", 
    "description": "To aid in the identification and analysis of somatic hybrids between potato (Solanum tuberosum, dihaploid line PDH 40) and the non tuber-bearing wild species S. brevidens, a series of species-specific repetitive DNA sequences have been isolated. This was accomplished by making libraries of HaeIII-digested total DNA of S. tuberosum and S. brevidens, by cloning fragments into the SmaI site of plasmid pUC18 and transforming them into E. coli (JM83). The S. brevidens library consisted of 1,000 recombinant clones, and that of S. tuberosum, 700. Nitrocellulose filters with recombinant clones were hybridised to nick-translated total DNA of S. brevidens and also S. tuberosum, and, following autoradiography, clones that hybridised strongly to the DNA of only one of the species were chosen. Two highly repeated S. brevidens clones (pSB1, 400 bp and pSB7,210 bp), one highcopy-number s. tuberosum clone (pST10, 200 bp) and one low-copy-number sequence of S. tuberosum (pST3, 1.5 kbp) were selected for further analysis by Southern hybridisation to digested total DNA. Clone pSB7 gave a ladder pattern on hybridisation to EcoR1-digested total DNA of S. brevidens, with signals at multiples of 200 bp DNA. Using these probes it was possible to verify the hybridity of putative hybrids of dihaploid S. tuberosum and S.brevidens, and to confirm by Southern analysis and by slot blots the parental genome dosage of hexaploid hybrids (two s. brevidens: one S. tuberosum, and vice-versa). The S. tuberosum-specific probe, pSTIO, hybridised with DNA of three other tuber-bearing wild species (S. hjertingii, S. capsicibaccatum and S. berthaultii). A squash-blot procedure was developed using the probes that would allow early identification of somatic hybrid callus. There are a number of useful applications of such species-specific probes in the identification and analysis of somatic hybrids. ", 
    "genre": "research_article", 
    "id": "sg:pub.10.1007/bf00224231", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": false, 
    "isPartOf": [
      {
        "id": "sg:journal.1135804", 
        "issn": [
          "0040-5752", 
          "1432-2242"
        ], 
        "name": "Theoretical and Applied Genetics", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "5", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "80"
      }
    ], 
    "name": "Species-specific sequences in the genus Solanum: identification, characterization, and application to study somatic hybrids of S. brevidens and S. tuberosum", 
    "pagination": "693-698", 
    "productId": [
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1007/bf00224231"
        ]
      }, 
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "13ba13e08267d53aa75359635441f29c1472adb84ddd569cb88f959938e899f4"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1004139792"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "0145600"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "24221078"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1007/bf00224231", 
      "https://app.dimensions.ai/details/publication/pub.1004139792"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-15T09:02", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000375_0000000375/records_91459_00000000.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "http://link.springer.com/10.1007/BF00224231"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/bf00224231'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/bf00224231'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/bf00224231'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/bf00224231'


 

This table displays all metadata directly associated to this object as RDF triples.

211 TRIPLES      21 PREDICATES      60 URIs      21 LITERALS      9 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1007/bf00224231 schema:about anzsrc-for:06
2 anzsrc-for:0604
3 schema:author N3785957c5a0449a0a5c4f93c18a54ad3
4 schema:citation sg:pub.10.1007/bf00249133
5 sg:pub.10.1007/bf00249142
6 sg:pub.10.1007/bf00257846
7 sg:pub.10.1007/bf00257851
8 sg:pub.10.1007/bf00257854
9 sg:pub.10.1007/bf00261236
10 sg:pub.10.1007/bf00261242
11 sg:pub.10.1007/bf00262566
12 sg:pub.10.1007/bf00263264
13 sg:pub.10.1007/bf00263265
14 sg:pub.10.1007/bf00264265
15 sg:pub.10.1007/bf00264483
16 sg:pub.10.1007/bf00265343
17 sg:pub.10.1007/bf00266993
18 sg:pub.10.1007/bf00270973
19 sg:pub.10.1007/bf00272881
20 sg:pub.10.1007/bf00299763
21 sg:pub.10.1007/bf00303519
22 sg:pub.10.1007/bf00333260
23 sg:pub.10.1007/bf00339590
24 sg:pub.10.1007/bf02620975
25 sg:pub.10.1007/bf02712670
26 https://doi.org/10.1016/0003-2697(83)90418-9
27 https://doi.org/10.1016/0167-7799(88)90085-6
28 https://doi.org/10.1016/0168-9452(85)90195-5
29 https://doi.org/10.1016/0378-1119(82)90015-4
30 https://doi.org/10.1016/s0022-2836(75)80083-0
31 https://doi.org/10.1016/s0022-2836(77)80102-2
32 https://doi.org/10.1073/pnas.81.7.1991
33 https://doi.org/10.1073/pnas.85.22.8568
34 https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
35 schema:datePublished 1990-11
36 schema:datePublishedReg 1990-11-01
37 schema:description To aid in the identification and analysis of somatic hybrids between potato (Solanum tuberosum, dihaploid line PDH 40) and the non tuber-bearing wild species S. brevidens, a series of species-specific repetitive DNA sequences have been isolated. This was accomplished by making libraries of HaeIII-digested total DNA of S. tuberosum and S. brevidens, by cloning fragments into the SmaI site of plasmid pUC18 and transforming them into E. coli (JM83). The S. brevidens library consisted of 1,000 recombinant clones, and that of S. tuberosum, 700. Nitrocellulose filters with recombinant clones were hybridised to nick-translated total DNA of S. brevidens and also S. tuberosum, and, following autoradiography, clones that hybridised strongly to the DNA of only one of the species were chosen. Two highly repeated S. brevidens clones (pSB1, 400 bp and pSB7,210 bp), one highcopy-number s. tuberosum clone (pST10, 200 bp) and one low-copy-number sequence of S. tuberosum (pST3, 1.5 kbp) were selected for further analysis by Southern hybridisation to digested total DNA. Clone pSB7 gave a ladder pattern on hybridisation to EcoR1-digested total DNA of S. brevidens, with signals at multiples of 200 bp DNA. Using these probes it was possible to verify the hybridity of putative hybrids of dihaploid S. tuberosum and S.brevidens, and to confirm by Southern analysis and by slot blots the parental genome dosage of hexaploid hybrids (two s. brevidens: one S. tuberosum, and vice-versa). The S. tuberosum-specific probe, pSTIO, hybridised with DNA of three other tuber-bearing wild species (S. hjertingii, S. capsicibaccatum and S. berthaultii). A squash-blot procedure was developed using the probes that would allow early identification of somatic hybrid callus. There are a number of useful applications of such species-specific probes in the identification and analysis of somatic hybrids.
38 schema:genre research_article
39 schema:inLanguage en
40 schema:isAccessibleForFree false
41 schema:isPartOf N6609a3d7aad44db69a2a7f2327fd2341
42 N8760a9ccb2f74af1b13d3e1f9c741d25
43 sg:journal.1135804
44 schema:name Species-specific sequences in the genus Solanum: identification, characterization, and application to study somatic hybrids of S. brevidens and S. tuberosum
45 schema:pagination 693-698
46 schema:productId N173f321facc643b795942acee5dc6b3c
47 N27f513ddc12f4b908d6f18919c80d606
48 N5ba28f4f144a4127baf64367139d95fa
49 N8a4d7d21bb644fe888627f83b327f73e
50 Na4ea3fbfe05b4ba7af0f955b064598e3
51 schema:sameAs https://app.dimensions.ai/details/publication/pub.1004139792
52 https://doi.org/10.1007/bf00224231
53 schema:sdDatePublished 2019-04-15T09:02
54 schema:sdLicense https://scigraph.springernature.com/explorer/license/
55 schema:sdPublisher Na229d6144ac747b8ba842f5be5f7a6f6
56 schema:url http://link.springer.com/10.1007/BF00224231
57 sgo:license sg:explorer/license/
58 sgo:sdDataset articles
59 rdf:type schema:ScholarlyArticle
60 N173f321facc643b795942acee5dc6b3c schema:name dimensions_id
61 schema:value pub.1004139792
62 rdf:type schema:PropertyValue
63 N1c918ec7e6ba4d46bd2ea28d516fff25 rdf:first sg:person.0646364020.60
64 rdf:rest N576d9da06ede4646a886972018bd3c9d
65 N27ade0a9b00445cb88cc8361687c4b61 rdf:first sg:person.016427007024.24
66 rdf:rest rdf:nil
67 N27f513ddc12f4b908d6f18919c80d606 schema:name pubmed_id
68 schema:value 24221078
69 rdf:type schema:PropertyValue
70 N2bc0069e2c2a40568b08504c5d6267b2 rdf:first N60819e8a7280446b9d3bd06954ce83d9
71 rdf:rest N1c918ec7e6ba4d46bd2ea28d516fff25
72 N3785957c5a0449a0a5c4f93c18a54ad3 rdf:first sg:person.01235023666.54
73 rdf:rest N2bc0069e2c2a40568b08504c5d6267b2
74 N576d9da06ede4646a886972018bd3c9d rdf:first sg:person.01163262030.73
75 rdf:rest N27ade0a9b00445cb88cc8361687c4b61
76 N5ba28f4f144a4127baf64367139d95fa schema:name readcube_id
77 schema:value 13ba13e08267d53aa75359635441f29c1472adb84ddd569cb88f959938e899f4
78 rdf:type schema:PropertyValue
79 N60819e8a7280446b9d3bd06954ce83d9 schema:affiliation https://www.grid.ac/institutes/grid.418374.d
80 schema:familyName Thomas
81 schema:givenName M.
82 rdf:type schema:Person
83 N6609a3d7aad44db69a2a7f2327fd2341 schema:issueNumber 5
84 rdf:type schema:PublicationIssue
85 N8760a9ccb2f74af1b13d3e1f9c741d25 schema:volumeNumber 80
86 rdf:type schema:PublicationVolume
87 N8a4d7d21bb644fe888627f83b327f73e schema:name doi
88 schema:value 10.1007/bf00224231
89 rdf:type schema:PropertyValue
90 Na229d6144ac747b8ba842f5be5f7a6f6 schema:name Springer Nature - SN SciGraph project
91 rdf:type schema:Organization
92 Na4ea3fbfe05b4ba7af0f955b064598e3 schema:name nlm_unique_id
93 schema:value 0145600
94 rdf:type schema:PropertyValue
95 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
96 schema:name Biological Sciences
97 rdf:type schema:DefinedTerm
98 anzsrc-for:0604 schema:inDefinedTermSet anzsrc-for:
99 schema:name Genetics
100 rdf:type schema:DefinedTerm
101 sg:journal.1135804 schema:issn 0040-5752
102 1432-2242
103 schema:name Theoretical and Applied Genetics
104 rdf:type schema:Periodical
105 sg:person.01163262030.73 schema:affiliation https://www.grid.ac/institutes/grid.418374.d
106 schema:familyName Karp
107 schema:givenName A.
108 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01163262030.73
109 rdf:type schema:Person
110 sg:person.01235023666.54 schema:affiliation https://www.grid.ac/institutes/grid.418374.d
111 schema:familyName Pehu
112 schema:givenName E.
113 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01235023666.54
114 rdf:type schema:Person
115 sg:person.016427007024.24 schema:affiliation https://www.grid.ac/institutes/grid.418374.d
116 schema:familyName Jones
117 schema:givenName M. G. K.
118 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.016427007024.24
119 rdf:type schema:Person
120 sg:person.0646364020.60 schema:affiliation https://www.grid.ac/institutes/grid.418374.d
121 schema:familyName Poutala
122 schema:givenName T.
123 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0646364020.60
124 rdf:type schema:Person
125 sg:pub.10.1007/bf00249133 schema:sameAs https://app.dimensions.ai/details/publication/pub.1051020423
126 https://doi.org/10.1007/bf00249133
127 rdf:type schema:CreativeWork
128 sg:pub.10.1007/bf00249142 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013652536
129 https://doi.org/10.1007/bf00249142
130 rdf:type schema:CreativeWork
131 sg:pub.10.1007/bf00257846 schema:sameAs https://app.dimensions.ai/details/publication/pub.1052308495
132 https://doi.org/10.1007/bf00257846
133 rdf:type schema:CreativeWork
134 sg:pub.10.1007/bf00257851 schema:sameAs https://app.dimensions.ai/details/publication/pub.1023839362
135 https://doi.org/10.1007/bf00257851
136 rdf:type schema:CreativeWork
137 sg:pub.10.1007/bf00257854 schema:sameAs https://app.dimensions.ai/details/publication/pub.1006138863
138 https://doi.org/10.1007/bf00257854
139 rdf:type schema:CreativeWork
140 sg:pub.10.1007/bf00261236 schema:sameAs https://app.dimensions.ai/details/publication/pub.1025682307
141 https://doi.org/10.1007/bf00261236
142 rdf:type schema:CreativeWork
143 sg:pub.10.1007/bf00261242 schema:sameAs https://app.dimensions.ai/details/publication/pub.1037896514
144 https://doi.org/10.1007/bf00261242
145 rdf:type schema:CreativeWork
146 sg:pub.10.1007/bf00262566 schema:sameAs https://app.dimensions.ai/details/publication/pub.1019961829
147 https://doi.org/10.1007/bf00262566
148 rdf:type schema:CreativeWork
149 sg:pub.10.1007/bf00263264 schema:sameAs https://app.dimensions.ai/details/publication/pub.1051305995
150 https://doi.org/10.1007/bf00263264
151 rdf:type schema:CreativeWork
152 sg:pub.10.1007/bf00263265 schema:sameAs https://app.dimensions.ai/details/publication/pub.1003180120
153 https://doi.org/10.1007/bf00263265
154 rdf:type schema:CreativeWork
155 sg:pub.10.1007/bf00264265 schema:sameAs https://app.dimensions.ai/details/publication/pub.1019824553
156 https://doi.org/10.1007/bf00264265
157 rdf:type schema:CreativeWork
158 sg:pub.10.1007/bf00264483 schema:sameAs https://app.dimensions.ai/details/publication/pub.1041387838
159 https://doi.org/10.1007/bf00264483
160 rdf:type schema:CreativeWork
161 sg:pub.10.1007/bf00265343 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040126741
162 https://doi.org/10.1007/bf00265343
163 rdf:type schema:CreativeWork
164 sg:pub.10.1007/bf00266993 schema:sameAs https://app.dimensions.ai/details/publication/pub.1033829529
165 https://doi.org/10.1007/bf00266993
166 rdf:type schema:CreativeWork
167 sg:pub.10.1007/bf00270973 schema:sameAs https://app.dimensions.ai/details/publication/pub.1005041642
168 https://doi.org/10.1007/bf00270973
169 rdf:type schema:CreativeWork
170 sg:pub.10.1007/bf00272881 schema:sameAs https://app.dimensions.ai/details/publication/pub.1078815979
171 https://doi.org/10.1007/bf00272881
172 rdf:type schema:CreativeWork
173 sg:pub.10.1007/bf00299763 schema:sameAs https://app.dimensions.ai/details/publication/pub.1000677365
174 https://doi.org/10.1007/bf00299763
175 rdf:type schema:CreativeWork
176 sg:pub.10.1007/bf00303519 schema:sameAs https://app.dimensions.ai/details/publication/pub.1024143166
177 https://doi.org/10.1007/bf00303519
178 rdf:type schema:CreativeWork
179 sg:pub.10.1007/bf00333260 schema:sameAs https://app.dimensions.ai/details/publication/pub.1043286327
180 https://doi.org/10.1007/bf00333260
181 rdf:type schema:CreativeWork
182 sg:pub.10.1007/bf00339590 schema:sameAs https://app.dimensions.ai/details/publication/pub.1004424855
183 https://doi.org/10.1007/bf00339590
184 rdf:type schema:CreativeWork
185 sg:pub.10.1007/bf02620975 schema:sameAs https://app.dimensions.ai/details/publication/pub.1049162310
186 https://doi.org/10.1007/bf02620975
187 rdf:type schema:CreativeWork
188 sg:pub.10.1007/bf02712670 schema:sameAs https://app.dimensions.ai/details/publication/pub.1003131267
189 https://doi.org/10.1007/bf02712670
190 rdf:type schema:CreativeWork
191 https://doi.org/10.1016/0003-2697(83)90418-9 schema:sameAs https://app.dimensions.ai/details/publication/pub.1012973560
192 rdf:type schema:CreativeWork
193 https://doi.org/10.1016/0167-7799(88)90085-6 schema:sameAs https://app.dimensions.ai/details/publication/pub.1002992948
194 rdf:type schema:CreativeWork
195 https://doi.org/10.1016/0168-9452(85)90195-5 schema:sameAs https://app.dimensions.ai/details/publication/pub.1030957608
196 rdf:type schema:CreativeWork
197 https://doi.org/10.1016/0378-1119(82)90015-4 schema:sameAs https://app.dimensions.ai/details/publication/pub.1050786151
198 rdf:type schema:CreativeWork
199 https://doi.org/10.1016/s0022-2836(75)80083-0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1033296365
200 rdf:type schema:CreativeWork
201 https://doi.org/10.1016/s0022-2836(77)80102-2 schema:sameAs https://app.dimensions.ai/details/publication/pub.1010792696
202 rdf:type schema:CreativeWork
203 https://doi.org/10.1073/pnas.81.7.1991 schema:sameAs https://app.dimensions.ai/details/publication/pub.1034589758
204 rdf:type schema:CreativeWork
205 https://doi.org/10.1073/pnas.85.22.8568 schema:sameAs https://app.dimensions.ai/details/publication/pub.1038254146
206 rdf:type schema:CreativeWork
207 https://doi.org/10.1111/j.1399-3054.1962.tb08052.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1023703429
208 rdf:type schema:CreativeWork
209 https://www.grid.ac/institutes/grid.418374.d schema:alternateName Rothamsted Research
210 schema:name Department of Biochemistry and Physiology, AFRC Institute of Arable Crops Research, Rothamsted Experimental Station, AL5 2JQ, Harpenden, Herts., UK
211 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...