Phylogenetic relationships within Hevea brasiliensis as deduced from a polymorphic mitochondrial DNA region View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1995-11

AUTHORS

H. Luo, M. Boutry

ABSTRACT

We have cloned a 4.5-kb mtDNA fragment showing a high RFLP polymorphism between various Hevea genotypes. Subcloning and sequencing of a 1.4-kb segment of this clone allowed us to design PCR amplification primers to isolate homologous mtDNA segments of about 0.9 kb from 23 representative genotypes of Hevea. Complete sequences from 4 genotypes showed between 6.7% and 20.2% of nucleotide diversity, suggesting the presence of a hypervariable, or hotspot, region. A sequence of 345 nucleotides within this region was determined for the 23 genotypes. The phylogenetic relationships inferred from the sequence comparison are in general agreement with the results obtained from mtDNA RFLP analysis, indicating that this polymorphic mtDNA region is a useful molecular marker for phylogenetic analysis within Hevea. More... »

PAGES

876-884

Journal

TITLE

Theoretical and Applied Genetics

ISSUE

6-7

VOLUME

91

Author Affiliations

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf00223895

DOI

http://dx.doi.org/10.1007/bf00223895

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1030645049

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/24169972


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