Somatic hybridization between Dianthus chinensis and D. barbatus through protoplast fusion View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1993-03

AUTHORS

M. Nakano, M. Mii

ABSTRACT

Protoplasts isolated from leaf mesophyll cells of Dianthus chinensis and D. barbatus were fused by polyethylene glycol (PEG). Calli exhibiting vigorous growth were selected from the PEG-treated protoplasts and shoots were regenerated from one of these calli after 5 months of culture. These shoots readily rooted and continuously produced flowers in the in-vitro condition. The data on flower color, chromosome number, and esterase isozyme patterns indicated that this plantlet was an interspecific somatic hybrid. The hybridity of the plantlet was also confirmed by nuclear rDNA analysis. This report provides the possibility of applying the somatic hybridization technique for the genetic improvement of the genus Dianthus. More... »

PAGES

1-5

References to SciGraph publications

Journal

TITLE

Theoretical and Applied Genetics

ISSUE

1

VOLUME

86

Author Affiliations

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf00223802

DOI

http://dx.doi.org/10.1007/bf00223802

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1038990046

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/24193377


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