Linkage between an isozyme marker and a restorer gene in radish cytoplasmic male sterility of rapeseed (Brassica napus L.) View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1992-11

AUTHORS

R. Delourme, F. Eber

ABSTRACT

Co-segregation studies of isozyme markers and male fertility restoration showed that a restorer gene from radish was introduced into rapeseed along with an isozyme marker (Pgi-2). The radish chromosome segment carrying these genes was introgressed into rapeseed through homoeologous recombination, substituting for some of the rapeseed alleles. By crossing heterozygous restored plants to male-sterile lines and to maintainers, tight linkage was found between the restorer gene and the marker. The recombination fraction was estimated at 0.25 ± 0.02%. Although few restored plants lacked the radish isozyme marker, it was still possible to distinguish male-fertile from male-sterile plants by their PGI-2 patterns. Furthermore, homozygous and heterozygous restored plants could be separated by specific PGI-2 phenotypes. Thus, the Pgi-2 marker is now currently used in restorer breeding programs. More... »

PAGES

222-228

Journal

TITLE

Theoretical and Applied Genetics

ISSUE

2-3

VOLUME

85

Author Affiliations

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf00222863

DOI

http://dx.doi.org/10.1007/bf00222863

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1028822946

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/24197308


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