Isolation of a new cDNA clone encoding an Rh polypeptide associated with the Rh blood group system View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1993-03

AUTHORS

Eiji Kajii, Fuminori Umenishi, Sadahiko Iwamoto, Shigenori Ikemoto

ABSTRACT

The polymerase chain reaction (PCR) was used to amplify Rh-related cDNAs from erythroid cells cultured by the selective two-phase liquid culture system for human erythroid progenitors in peripheral blood. Direct sequencing based on PCR presents heterozygous bands. Two Rh polypeptide cDNAs have been isolated from the PCR products and tentatively designated RhPI cDNA and RhPII cDNA. Both cDNA clones have an open reading frame composed of 1251 nucleotides. The RhPI cDNA clone shows a single nucleotide substitution with no amino acid substitution compared with the published sequence. The RhPII cDNA clone, on the other hand, differs from the above by 41 nucleotide substitutions with the open reading frame, resulting in 31 amino acid substitutions. More... »

PAGES

157-162

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf00222717

DOI

http://dx.doi.org/10.1007/bf00222717

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1019952770

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/7916743


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