Identification and characterisation of the extracellular sucrases of Zymomonas mobilis UQM 2716 (ATCC 39676) View Full Text


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Article Info

DATE

1994-05

AUTHORS

R. G. Crittenden, H. W. Doelle

ABSTRACT

An investigation was conducted to isolate, and characterise the extracellular sucrases of Zymomonas mobilis UQM 2716. Levansucrase (EC 2.4.1.10) was the only extracellular sucrase produced by this organism. This enzyme was responsible for sucrose hydrolysis, levan formation, and oligosaccharide production. It had a molecular mass of 98 kDa, a Michaelis constant (Km) of 64 mm, and a pH optimum of 5.5. It was inhibited by glucose, but not by fructose, ethanol, sorbitol, NaCl, TRIS or ethylenediaminetetraacetic acid (EDTA). The formation of levan was the principal reaction catalysed by this enzyme at low temperatures. However, levan formation was thermolabile, being irreversibly lost when levansucrase was heated to 35°C. S This did not effect sucrose hydrolysis or oligosaccharide formation, which were optimal at 45°C. Sucrose concentration greatly influenced the type of acceptor molecule used in the transfructosylation reactions catalysed by levansucrase. At low sucrose concentration, the predominant reaction catalysed was the hydrolysis of sucrose to free glucose and fructose. At high sucrose concentrations, oligosaccharide production was the major reaction catalysed. More... »

PAGES

302-308

References to SciGraph publications

  • 1984-04. Formation of levan and sorbitol from sucrose by Zymomonas mobilis in APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
  • 1985-10. Fermentation pattern of Zymomonas mobilis at high sucrose concentrations in APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
  • 1993-10. Structural identification of oligosaccharides produced by Zymomonas mobilis levansucrase in BIOTECHNOLOGY LETTERS
  • 1985-10. The production of ethanol from sucrose using Zymomonas mobilis in APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
  • 1991. Byproducts from Zymomonas mobilis in BIOREACTOR SYSTEMS AND EFFECTS
  • 1991-04. Kinetic control of ethanol production by Zymomonas mobilis in APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
  • 1990-11. The relationship between sucrose hydrolysis, sorbitol formation and mineral ion concentration during bioethanol formation using Zymomonas mobilis 2716 in APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
  • 1992-04. The effects of potassium and chloride ions on the ethanolic fermentation of sucrose by Zymomonas mobilis 2716 in APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
  • 1986-02. Rate and yield limiting factors in continuous fermentation of sucrose byZymomonas mobilis in BIOTECHNOLOGY LETTERS
  • 1993-07. Stability and expression of a cloned α-glucosidase gene inZymomonas mobilis grown in batch and continuous culture in BIOTECHNOLOGY LETTERS
  • 1990-01. Characterization of sucrose-hydrolyzing enzymes of Zymomonas mobilis in ARCHIVES OF MICROBIOLOGY
  • 1991-02. Scale-up of ethanol production from sugarcane usingZymomonas mobilis in BIOTECHNOLOGY LETTERS
  • 1991-05. Production of 1-kestose with intact mycelium of Aspergillus phoenicis containing sucrose-1F-fructosyltransferase in APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
  • 1983-05. Levansucrase from Zymomonas mobilis in BIOTECHNOLOGY LETTERS
  • 1980-12. Effect of temperature on sucrose to ethanol conversion by Zymomonas mobilis strains in BIOTECHNOLOGY LETTERS
  • 1991-02. Identification of saccharolytic enzymes ofZymomonas mobilis CP4 in BIOTECHNOLOGY LETTERS
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