Preparation and characterization of monoclonal antibodies to human hexokinase type I View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1990-09

AUTHORS

Laura Chiarantini, Giordano Serafini, Vilberto Stocchi, Mauro Magnani

ABSTRACT

1. Three different immunization protocols and several screening procedures were used to prepare seven mouse monoclonal antibodies to human placenta hexokinase type I. None of these monoclonals were able to recognize the native enzyme but all detected hexokinase when adsorbed onto polystyrene plates or on immunoblots after SDS/polyacrylamide-gel electrophoresis. 2. All seven monoclonals recognize the two different subtypes of human hexokinase I equally well. Limited tryptic digestion of hexokinase followed by Western blotting and immunodetection show that these monoclonals recognize epitopes that lie in different tryptic peptides. 3. Comparative ELISA studies showed that human hexokinase types I and II have great immunological similarities while hexokinase I from different mammalian species and yeast hexokinase are recognized with different affinities. More... »

PAGES

145-151

References to SciGraph publications

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf00221056

DOI

http://dx.doi.org/10.1007/bf00221056

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1012340704

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/2280763


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