Immunocytochemical localization of nerve growth factor (NGF) in the submandibular gland of adult mice by light and electron microscopy View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1976-06

AUTHORS

M. E. Schwab, K. Stöckel, H. Thoenen

ABSTRACT

Nerve growth factor (NGF) was localized in the submandibular gland of adult male mice by a direct immunocytochemical method using highly purified antibodies against NGF coupled to horseradish peroxidase. In light microscopic sections the reaction product was entirely confined to the cells of the secretory tubules. The acinar part of the gland was free of reaction product. This finding was confirmed by electron microscopy. Within the cells NGF was localized exclusively in the apical secretory granules.No reaction was observed in the rough endoplasmic reticulum, the Golgi region or in the granules of the basal part of the cells. This observation favours the assumption that NGF is derived from a precursor molecule and that the precursor is transformed into immunologically active NGF within the secretory granules during their transport from the basal to the apical part of the tubular cells. Stimulation of the submandibular gland with carbachol (2 mg/kg) led to a massive release of the content of the secretory granules, including NGF, into the salivary duct. More... »

PAGES

289-299

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf00219602

DOI

http://dx.doi.org/10.1007/bf00219602

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1045897646

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/949726


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