Class I major histocompatibility complex cDNA clones from sheep thymus: alternative splicing could make a long cytoplasmic tail View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1990-08

AUTHORS

Dario Grossberger, Wayne Hein, Anne Marcuz

ABSTRACT

To investigate the class I major histocompatibility complex (MHC) genes expressed in the young sheep thymus, a cDNA library was screened with a human HLA-B7 cDNA probe under conditions of relaxed stringency. Thirteen clones were isolated and found by partial sequences to fall into five classes, requiring the expression of at least three loci. One sequence was found six times, almost half of the total, and may thus represent the major message expressed in the young sheep thymus. One of the clones was found to have failed to excise the intron between cytoplasmic exons 7 and 8, leading to the predicted synthesis of a cytoplasmic domain 23 amino acids longer than the other sheep sequences, and 15 amino acids longer than any cytoplasmic domain previously described. The sequences of all the clones were found to be most similar to bovine, and least similar to mouse class I MHC sequences. More... »

PAGES

77-87

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf00210444

DOI

http://dx.doi.org/10.1007/bf00210444

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1003041737

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/2397935


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