Reappearance of hydrolytic activities and tonoplast proteins in the regenerated vacuole of evacuolated protoplasts View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1992-04

AUTHORS

Stefan Hörtensteiner, Enrico Martinoia, Nikolaus Amrhein

ABSTRACT

Mesophyll protoplasts of tobacco (Nicotiana tabacum L. cv. Xanthi) were evacuolated by centrifugation in a density gradient. Evacuolation resulted in the quantitative loss of vacuolar hydrolytic activities. The evacuolated miniprotoplasts were cultivated under different conditions, and the regeneration of the central vacuole was investigated by light and electron microscopy as well as by the determination of activities of vacuolar marker enzymes. Vacuoles and hydrolytic activities, as well as cell wall material reappeared faster when the cells were cultivated at low osmotic strength. A newly synthesized tonoplast polypeptide could be detected using a polyspecific serum raised against tonoplast proteins of barley (Hordeum vulgare L.). Both vacuolar proton pumps, the ATPase as well as the pyrophosphatase appear to be newly synthesized during the regeneration of the vacuole. More... »

PAGES

113-121

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf00201632

DOI

http://dx.doi.org/10.1007/bf00201632

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1038146385

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/24177975


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biochemistry and Cell Biology", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Institut f\u00fcr Pflanzenwissenschaften, ETH Z\u00fcrich, Sonneggstrasse 5, CH-8092, Z\u00fcrich, Switzerland", 
          "id": "http://www.grid.ac/institutes/grid.5801.c", 
          "name": [
            "Institut f\u00fcr Pflanzenwissenschaften, ETH Z\u00fcrich, Sonneggstrasse 5, CH-8092, Z\u00fcrich, Switzerland"
          ], 
          "type": "Organization"
        }, 
        "familyName": "H\u00f6rtensteiner", 
        "givenName": "Stefan", 
        "id": "sg:person.01354306505.27", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01354306505.27"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Institut f\u00fcr Pflanzenwissenschaften, ETH Z\u00fcrich, Sonneggstrasse 5, CH-8092, Z\u00fcrich, Switzerland", 
          "id": "http://www.grid.ac/institutes/grid.5801.c", 
          "name": [
            "Institut f\u00fcr Pflanzenwissenschaften, ETH Z\u00fcrich, Sonneggstrasse 5, CH-8092, Z\u00fcrich, Switzerland"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Martinoia", 
        "givenName": "Enrico", 
        "id": "sg:person.01011612624.98", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01011612624.98"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Institut f\u00fcr Pflanzenwissenschaften, ETH Z\u00fcrich, Sonneggstrasse 5, CH-8092, Z\u00fcrich, Switzerland", 
          "id": "http://www.grid.ac/institutes/grid.5801.c", 
          "name": [
            "Institut f\u00fcr Pflanzenwissenschaften, ETH Z\u00fcrich, Sonneggstrasse 5, CH-8092, Z\u00fcrich, Switzerland"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Amrhein", 
        "givenName": "Nikolaus", 
        "id": "sg:person.0663624415.92", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0663624415.92"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "sg:pub.10.1038/227680a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1010419937", 
          "https://doi.org/10.1038/227680a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/289292a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1031536233", 
          "https://doi.org/10.1038/289292a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00508141", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1043412312", 
          "https://doi.org/10.1007/bf00508141"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00385097", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1028757873", 
          "https://doi.org/10.1007/bf00385097"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf01287680", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1052532186", 
          "https://doi.org/10.1007/bf01287680"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00393689", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1039865685", 
          "https://doi.org/10.1007/bf00393689"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "1992-04", 
    "datePublishedReg": "1992-04-01", 
    "description": "Mesophyll protoplasts of tobacco (Nicotiana tabacum L. cv. Xanthi) were evacuolated by centrifugation in a density gradient. Evacuolation resulted in the quantitative loss of vacuolar hydrolytic activities. The evacuolated miniprotoplasts were cultivated under different conditions, and the regeneration of the central vacuole was investigated by light and electron microscopy as well as by the determination of activities of vacuolar marker enzymes. Vacuoles and hydrolytic activities, as well as cell wall material reappeared faster when the cells were cultivated at low osmotic strength. A newly synthesized tonoplast polypeptide could be detected using a polyspecific serum raised against tonoplast proteins of barley (Hordeum vulgare L.). Both vacuolar proton pumps, the ATPase as well as the pyrophosphatase appear to be newly synthesized during the regeneration of the vacuole.", 
    "genre": "article", 
    "id": "sg:pub.10.1007/bf00201632", 
    "isAccessibleForFree": false, 
    "isPartOf": [
      {
        "id": "sg:journal.1054035", 
        "issn": [
          "0032-0935", 
          "1432-2048"
        ], 
        "name": "Planta", 
        "publisher": "Springer Nature", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "1", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "187"
      }
    ], 
    "keywords": [
      "polyspecific sera", 
      "marker enzymes", 
      "vacuolar proton pump", 
      "activity", 
      "determination of activity", 
      "vacuoles", 
      "serum", 
      "hydrolytic activity", 
      "low osmotic strength", 
      "protein", 
      "regeneration", 
      "tobacco", 
      "cells", 
      "reappearance", 
      "osmotic strength", 
      "ATPase", 
      "proton pump", 
      "loss", 
      "centrifugation", 
      "enzyme", 
      "quantitative loss", 
      "polypeptide", 
      "density gradient", 
      "pump", 
      "electron microscopy", 
      "conditions", 
      "different conditions", 
      "microscopy", 
      "pyrophosphatase", 
      "miniprotoplasts", 
      "materials", 
      "determination", 
      "light", 
      "mesophyll protoplasts", 
      "central vacuole", 
      "strength", 
      "gradient", 
      "protoplasts", 
      "evacuolation", 
      "barley", 
      "cell wall material", 
      "wall material", 
      "tonoplast polypeptides", 
      "tonoplast proteins"
    ], 
    "name": "Reappearance of hydrolytic activities and tonoplast proteins in the regenerated vacuole of evacuolated protoplasts", 
    "pagination": "113-121", 
    "productId": [
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1038146385"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1007/bf00201632"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "24177975"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1007/bf00201632", 
      "https://app.dimensions.ai/details/publication/pub.1038146385"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2022-12-01T06:21", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-springernature-scigraph/baseset/20221201/entities/gbq_results/article/article_258.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://doi.org/10.1007/bf00201632"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/bf00201632'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/bf00201632'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/bf00201632'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/bf00201632'


 

This table displays all metadata directly associated to this object as RDF triples.

143 TRIPLES      21 PREDICATES      76 URIs      62 LITERALS      7 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1007/bf00201632 schema:about anzsrc-for:06
2 anzsrc-for:0601
3 schema:author N8a62ddb263b241d49a5cb8fbbcff30b9
4 schema:citation sg:pub.10.1007/bf00385097
5 sg:pub.10.1007/bf00393689
6 sg:pub.10.1007/bf00508141
7 sg:pub.10.1007/bf01287680
8 sg:pub.10.1038/227680a0
9 sg:pub.10.1038/289292a0
10 schema:datePublished 1992-04
11 schema:datePublishedReg 1992-04-01
12 schema:description Mesophyll protoplasts of tobacco (Nicotiana tabacum L. cv. Xanthi) were evacuolated by centrifugation in a density gradient. Evacuolation resulted in the quantitative loss of vacuolar hydrolytic activities. The evacuolated miniprotoplasts were cultivated under different conditions, and the regeneration of the central vacuole was investigated by light and electron microscopy as well as by the determination of activities of vacuolar marker enzymes. Vacuoles and hydrolytic activities, as well as cell wall material reappeared faster when the cells were cultivated at low osmotic strength. A newly synthesized tonoplast polypeptide could be detected using a polyspecific serum raised against tonoplast proteins of barley (Hordeum vulgare L.). Both vacuolar proton pumps, the ATPase as well as the pyrophosphatase appear to be newly synthesized during the regeneration of the vacuole.
13 schema:genre article
14 schema:isAccessibleForFree false
15 schema:isPartOf N8269a2dad72048c8aa2c9b65a9bbf536
16 Nc1751bbd6495406ba32ef85e4c3da49d
17 sg:journal.1054035
18 schema:keywords ATPase
19 activity
20 barley
21 cell wall material
22 cells
23 central vacuole
24 centrifugation
25 conditions
26 density gradient
27 determination
28 determination of activity
29 different conditions
30 electron microscopy
31 enzyme
32 evacuolation
33 gradient
34 hydrolytic activity
35 light
36 loss
37 low osmotic strength
38 marker enzymes
39 materials
40 mesophyll protoplasts
41 microscopy
42 miniprotoplasts
43 osmotic strength
44 polypeptide
45 polyspecific sera
46 protein
47 proton pump
48 protoplasts
49 pump
50 pyrophosphatase
51 quantitative loss
52 reappearance
53 regeneration
54 serum
55 strength
56 tobacco
57 tonoplast polypeptides
58 tonoplast proteins
59 vacuolar proton pump
60 vacuoles
61 wall material
62 schema:name Reappearance of hydrolytic activities and tonoplast proteins in the regenerated vacuole of evacuolated protoplasts
63 schema:pagination 113-121
64 schema:productId N45844c6b7fff4ea08b78f2022a3fba32
65 N8a3968cb65cd40f782841c51a6b4541f
66 N9305343b80144643aea7e427be189f73
67 schema:sameAs https://app.dimensions.ai/details/publication/pub.1038146385
68 https://doi.org/10.1007/bf00201632
69 schema:sdDatePublished 2022-12-01T06:21
70 schema:sdLicense https://scigraph.springernature.com/explorer/license/
71 schema:sdPublisher Ncfc0939db0cd429c932c302afed371bc
72 schema:url https://doi.org/10.1007/bf00201632
73 sgo:license sg:explorer/license/
74 sgo:sdDataset articles
75 rdf:type schema:ScholarlyArticle
76 N1f06f59f3cec485da4b5307719067653 rdf:first sg:person.01011612624.98
77 rdf:rest Ndf210eab28e546a192481312f600fd27
78 N45844c6b7fff4ea08b78f2022a3fba32 schema:name dimensions_id
79 schema:value pub.1038146385
80 rdf:type schema:PropertyValue
81 N8269a2dad72048c8aa2c9b65a9bbf536 schema:volumeNumber 187
82 rdf:type schema:PublicationVolume
83 N8a3968cb65cd40f782841c51a6b4541f schema:name doi
84 schema:value 10.1007/bf00201632
85 rdf:type schema:PropertyValue
86 N8a62ddb263b241d49a5cb8fbbcff30b9 rdf:first sg:person.01354306505.27
87 rdf:rest N1f06f59f3cec485da4b5307719067653
88 N9305343b80144643aea7e427be189f73 schema:name pubmed_id
89 schema:value 24177975
90 rdf:type schema:PropertyValue
91 Nc1751bbd6495406ba32ef85e4c3da49d schema:issueNumber 1
92 rdf:type schema:PublicationIssue
93 Ncfc0939db0cd429c932c302afed371bc schema:name Springer Nature - SN SciGraph project
94 rdf:type schema:Organization
95 Ndf210eab28e546a192481312f600fd27 rdf:first sg:person.0663624415.92
96 rdf:rest rdf:nil
97 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
98 schema:name Biological Sciences
99 rdf:type schema:DefinedTerm
100 anzsrc-for:0601 schema:inDefinedTermSet anzsrc-for:
101 schema:name Biochemistry and Cell Biology
102 rdf:type schema:DefinedTerm
103 sg:journal.1054035 schema:issn 0032-0935
104 1432-2048
105 schema:name Planta
106 schema:publisher Springer Nature
107 rdf:type schema:Periodical
108 sg:person.01011612624.98 schema:affiliation grid-institutes:grid.5801.c
109 schema:familyName Martinoia
110 schema:givenName Enrico
111 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01011612624.98
112 rdf:type schema:Person
113 sg:person.01354306505.27 schema:affiliation grid-institutes:grid.5801.c
114 schema:familyName Hörtensteiner
115 schema:givenName Stefan
116 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01354306505.27
117 rdf:type schema:Person
118 sg:person.0663624415.92 schema:affiliation grid-institutes:grid.5801.c
119 schema:familyName Amrhein
120 schema:givenName Nikolaus
121 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0663624415.92
122 rdf:type schema:Person
123 sg:pub.10.1007/bf00385097 schema:sameAs https://app.dimensions.ai/details/publication/pub.1028757873
124 https://doi.org/10.1007/bf00385097
125 rdf:type schema:CreativeWork
126 sg:pub.10.1007/bf00393689 schema:sameAs https://app.dimensions.ai/details/publication/pub.1039865685
127 https://doi.org/10.1007/bf00393689
128 rdf:type schema:CreativeWork
129 sg:pub.10.1007/bf00508141 schema:sameAs https://app.dimensions.ai/details/publication/pub.1043412312
130 https://doi.org/10.1007/bf00508141
131 rdf:type schema:CreativeWork
132 sg:pub.10.1007/bf01287680 schema:sameAs https://app.dimensions.ai/details/publication/pub.1052532186
133 https://doi.org/10.1007/bf01287680
134 rdf:type schema:CreativeWork
135 sg:pub.10.1038/227680a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1010419937
136 https://doi.org/10.1038/227680a0
137 rdf:type schema:CreativeWork
138 sg:pub.10.1038/289292a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1031536233
139 https://doi.org/10.1038/289292a0
140 rdf:type schema:CreativeWork
141 grid-institutes:grid.5801.c schema:alternateName Institut für Pflanzenwissenschaften, ETH Zürich, Sonneggstrasse 5, CH-8092, Zürich, Switzerland
142 schema:name Institut für Pflanzenwissenschaften, ETH Zürich, Sonneggstrasse 5, CH-8092, Zürich, Switzerland
143 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...