Tumour inhibitory effects of TCGF/IL-2/-containing preparations View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1985-02

AUTHORS

J. Bubeník, M. Indrová, P. Perlmann, K. Berzins, O. Mach, J. Kraml, A. Toulcová

ABSTRACT

Supernatants from ConA-stimulated rat spleen cell cultures and from cultures of PMA-stimulated murine lymphoma subline EL-4TF were found to contain TCGF and to inhibit growth of a transplantable, MC-induced sarcoma MC11 in syngeneic mice. Tumour-inhibitory effects of the supernatants were dependent on local and repeated administration. Prior to use of the supernatants obtained from PMA-stimulated EL-4TF cell cultures, the dialysable PMA had to be removed; contamination with PMA was found to abolish the tumour-inhibitory effect of the supernatants and to produce enhancement of tumour growth. A significant tumour-inhibitory effect has also been obtained with partially purified TCGF prepared from culture supernatants of cloned EL-4TF cells by ammonium sulphate precipitation, ion-exchange (FPLC) chromatography, and AcA 44 Ultrogel filtration. More... »

PAGES

57-61

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf00199313

DOI

http://dx.doi.org/10.1007/bf00199313

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1050374568

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/3872165


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