High molecular mass glycoproteins associated with the siliceous scales and bristles of Mallomonas splendens (Synurophyceae) may be involved in cell ... View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1996-06

AUTHORS

Martha Ludwig, Jan L. Lind, Elizabeth A. Miller, Richard Wetherbee

ABSTRACT

The elaborate scale case of Mallomonas splendens (Synurophyceae) consists of an overlapping arrangement of siliceous scales. In addition, siliceous bristles are attached to specialized base plate scales located at both the anterior and posterior ends of the cells. We have generated monoclonal antibodies against molecules associated with the scale case of M. splendens. One of these antibodies, designated MsS.H9, labelled a proteinaceous epitope of high-molecular-mass cell surface glycoproteins. Immunofluorescence and immunoelectron microscopy demonstrated that only two regions of M. splendens scale cases were labelled by MsS.H9, namely, the upper surface of the scales that contact neighboring scales and the bases of the bristles. Immunoelectron microscopy using thin sections of M. splendens cells showed these labelling sites corresponded to the amorphous material at the sites of scale-to-scale overlap and to a fibrillar complex located at scale-to-bristle attachment sites. Scales and bristles of M. splendens are formed within the cell, in silica deposition vesicles. Immunolabelling of cell sections containing developing scales and bristles showed that MsS.H9 labelling sites were present very early in the formation of these cell surface components. MsS.H9 labelling was also found associated with developing flagellar hairs whereas no labelling was detected on these structures after their deployment onto the flagellum. The location of MsS.H9 labelling sites strongly suggests that the molecule(s) recognized by the antibody plays a role in the adhesion of the individual components making up the scale case of M. splendens. More... »

PAGES

219-228

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf00196562

DOI

http://dx.doi.org/10.1007/bf00196562

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1023431946


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biochemistry and Cell Biology", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "School of Botany, The University of Melbourne, 3052, Parkville, Victoria, Australia", 
          "id": "http://www.grid.ac/institutes/grid.1008.9", 
          "name": [
            "School of Botany, The University of Melbourne, 3052, Parkville, Victoria, Australia"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Ludwig", 
        "givenName": "Martha", 
        "id": "sg:person.01262371225.25", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01262371225.25"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "School of Botany, The University of Melbourne, 3052, Parkville, Victoria, Australia", 
          "id": "http://www.grid.ac/institutes/grid.1008.9", 
          "name": [
            "School of Botany, The University of Melbourne, 3052, Parkville, Victoria, Australia"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Lind", 
        "givenName": "Jan L.", 
        "id": "sg:person.01263633521.09", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01263633521.09"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "School of Botany, The University of Melbourne, 3052, Parkville, Victoria, Australia", 
          "id": "http://www.grid.ac/institutes/grid.1008.9", 
          "name": [
            "School of Botany, The University of Melbourne, 3052, Parkville, Victoria, Australia"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Miller", 
        "givenName": "Elizabeth A.", 
        "id": "sg:person.0627102647.36", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0627102647.36"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "School of Botany, The University of Melbourne, 3052, Parkville, Victoria, Australia", 
          "id": "http://www.grid.ac/institutes/grid.1008.9", 
          "name": [
            "School of Botany, The University of Melbourne, 3052, Parkville, Victoria, Australia"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Wetherbee", 
        "givenName": "Richard", 
        "id": "sg:person.01224222341.91", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01224222341.91"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "sg:pub.10.1007/bf00239985", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1050413047", 
          "https://doi.org/10.1007/bf00239985"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/978-1-4612-5944-2_8", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1003560034", 
          "https://doi.org/10.1007/978-1-4612-5944-2_8"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00348902", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1043888630", 
          "https://doi.org/10.1007/bf00348902"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf01247899", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1044629391", 
          "https://doi.org/10.1007/bf01247899"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf01281167", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1052825704", 
          "https://doi.org/10.1007/bf01281167"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf01666387", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1021387866", 
          "https://doi.org/10.1007/bf01666387"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf01666400", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1002593176", 
          "https://doi.org/10.1007/bf01666400"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/227680a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1010419937", 
          "https://doi.org/10.1038/227680a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00195889", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1014420218", 
          "https://doi.org/10.1007/bf00195889"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "1996-06", 
    "datePublishedReg": "1996-06-01", 
    "description": "The elaborate scale case of Mallomonas splendens (Synurophyceae) consists of an overlapping arrangement of siliceous scales. In addition, siliceous bristles are attached to specialized base plate scales located at both the anterior and posterior ends of the cells. We have generated monoclonal antibodies against molecules associated with the scale case of M. splendens. One of these antibodies, designated MsS.H9, labelled a proteinaceous epitope of high-molecular-mass cell surface glycoproteins. Immunofluorescence and immunoelectron microscopy demonstrated that only two regions of M. splendens scale cases were labelled by MsS.H9, namely, the upper surface of the scales that contact neighboring scales and the bases of the bristles. Immunoelectron microscopy using thin sections of M. splendens cells showed these labelling sites corresponded to the amorphous material at the sites of scale-to-scale overlap and to a fibrillar complex located at scale-to-bristle attachment sites. Scales and bristles of M. splendens are formed within the cell, in silica deposition vesicles. Immunolabelling of cell sections containing developing scales and bristles showed that MsS.H9 labelling sites were present very early in the formation of these cell surface components. MsS.H9 labelling was also found associated with developing flagellar hairs whereas no labelling was detected on these structures after their deployment onto the flagellum. The location of MsS.H9 labelling sites strongly suggests that the molecule(s) recognized by the antibody plays a role in the adhesion of the individual components making up the scale case of M. splendens.", 
    "genre": "article", 
    "id": "sg:pub.10.1007/bf00196562", 
    "isAccessibleForFree": false, 
    "isPartOf": [
      {
        "id": "sg:journal.1054035", 
        "issn": [
          "0032-0935", 
          "1432-2048"
        ], 
        "name": "Planta", 
        "publisher": "Springer Nature", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "2", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "199"
      }
    ], 
    "keywords": [
      "scale case", 
      "upper surface", 
      "surface development", 
      "amorphous materials", 
      "neighboring scales", 
      "microscopy", 
      "surface", 
      "individual components", 
      "materials", 
      "adhesion", 
      "components", 
      "scale", 
      "structure", 
      "thin sections", 
      "sections", 
      "plate scale", 
      "deployment", 
      "surface components", 
      "arrangement", 
      "location", 
      "formation", 
      "cases", 
      "addition", 
      "bristles", 
      "cell sections", 
      "sites", 
      "region", 
      "maintenance", 
      "overlapping arrangement", 
      "development", 
      "end", 
      "basis", 
      "cells", 
      "immunoelectron microscopy", 
      "fibrillar complex", 
      "monoclonal antibodies", 
      "cell surface glycoprotein", 
      "antibodies", 
      "silica deposition vesicle", 
      "cell surface components", 
      "scale overlap", 
      "high molecular mass glycoproteins", 
      "surface glycoprotein", 
      "attachment sites", 
      "molecules", 
      "overlap", 
      "mass glycoproteins", 
      "glycoprotein", 
      "siliceous scales", 
      "H9", 
      "epitopes", 
      "posterior end", 
      "labeling", 
      "role", 
      "proteinaceous epitopes", 
      "hair", 
      "labeling sites", 
      "vesicles", 
      "M. splendens", 
      "complexes", 
      "splendens", 
      "flagella", 
      "flagellar hairs"
    ], 
    "name": "High molecular mass glycoproteins associated with the siliceous scales and bristles of Mallomonas splendens (Synurophyceae) may be involved in cell surface development and maintenance", 
    "pagination": "219-228", 
    "productId": [
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1023431946"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1007/bf00196562"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1007/bf00196562", 
      "https://app.dimensions.ai/details/publication/pub.1023431946"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2022-09-02T15:49", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-springernature-scigraph/baseset/20220902/entities/gbq_results/article/article_305.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://doi.org/10.1007/bf00196562"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/bf00196562'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/bf00196562'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/bf00196562'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/bf00196562'


 

This table displays all metadata directly associated to this object as RDF triples.

177 TRIPLES      21 PREDICATES      97 URIs      80 LITERALS      6 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1007/bf00196562 schema:about anzsrc-for:06
2 anzsrc-for:0601
3 schema:author N60bd60342eeb4ad5a9cf56684aa943c6
4 schema:citation sg:pub.10.1007/978-1-4612-5944-2_8
5 sg:pub.10.1007/bf00195889
6 sg:pub.10.1007/bf00239985
7 sg:pub.10.1007/bf00348902
8 sg:pub.10.1007/bf01247899
9 sg:pub.10.1007/bf01281167
10 sg:pub.10.1007/bf01666387
11 sg:pub.10.1007/bf01666400
12 sg:pub.10.1038/227680a0
13 schema:datePublished 1996-06
14 schema:datePublishedReg 1996-06-01
15 schema:description The elaborate scale case of Mallomonas splendens (Synurophyceae) consists of an overlapping arrangement of siliceous scales. In addition, siliceous bristles are attached to specialized base plate scales located at both the anterior and posterior ends of the cells. We have generated monoclonal antibodies against molecules associated with the scale case of M. splendens. One of these antibodies, designated MsS.H9, labelled a proteinaceous epitope of high-molecular-mass cell surface glycoproteins. Immunofluorescence and immunoelectron microscopy demonstrated that only two regions of M. splendens scale cases were labelled by MsS.H9, namely, the upper surface of the scales that contact neighboring scales and the bases of the bristles. Immunoelectron microscopy using thin sections of M. splendens cells showed these labelling sites corresponded to the amorphous material at the sites of scale-to-scale overlap and to a fibrillar complex located at scale-to-bristle attachment sites. Scales and bristles of M. splendens are formed within the cell, in silica deposition vesicles. Immunolabelling of cell sections containing developing scales and bristles showed that MsS.H9 labelling sites were present very early in the formation of these cell surface components. MsS.H9 labelling was also found associated with developing flagellar hairs whereas no labelling was detected on these structures after their deployment onto the flagellum. The location of MsS.H9 labelling sites strongly suggests that the molecule(s) recognized by the antibody plays a role in the adhesion of the individual components making up the scale case of M. splendens.
16 schema:genre article
17 schema:isAccessibleForFree false
18 schema:isPartOf N550f55e49de7456caa0532053885bf8d
19 N627b4b72c8a1433388b1dfd97cd00d41
20 sg:journal.1054035
21 schema:keywords H9
22 M. splendens
23 addition
24 adhesion
25 amorphous materials
26 antibodies
27 arrangement
28 attachment sites
29 basis
30 bristles
31 cases
32 cell sections
33 cell surface components
34 cell surface glycoprotein
35 cells
36 complexes
37 components
38 deployment
39 development
40 end
41 epitopes
42 fibrillar complex
43 flagella
44 flagellar hairs
45 formation
46 glycoprotein
47 hair
48 high molecular mass glycoproteins
49 immunoelectron microscopy
50 individual components
51 labeling
52 labeling sites
53 location
54 maintenance
55 mass glycoproteins
56 materials
57 microscopy
58 molecules
59 monoclonal antibodies
60 neighboring scales
61 overlap
62 overlapping arrangement
63 plate scale
64 posterior end
65 proteinaceous epitopes
66 region
67 role
68 scale
69 scale case
70 scale overlap
71 sections
72 silica deposition vesicle
73 siliceous scales
74 sites
75 splendens
76 structure
77 surface
78 surface components
79 surface development
80 surface glycoprotein
81 thin sections
82 upper surface
83 vesicles
84 schema:name High molecular mass glycoproteins associated with the siliceous scales and bristles of Mallomonas splendens (Synurophyceae) may be involved in cell surface development and maintenance
85 schema:pagination 219-228
86 schema:productId N38ba4a8aaee2407c8f1047abed9b87b6
87 Ne4395574030548dbab8a9505a7068d15
88 schema:sameAs https://app.dimensions.ai/details/publication/pub.1023431946
89 https://doi.org/10.1007/bf00196562
90 schema:sdDatePublished 2022-09-02T15:49
91 schema:sdLicense https://scigraph.springernature.com/explorer/license/
92 schema:sdPublisher Naceaa29b1c104355b5cb793b5ae71360
93 schema:url https://doi.org/10.1007/bf00196562
94 sgo:license sg:explorer/license/
95 sgo:sdDataset articles
96 rdf:type schema:ScholarlyArticle
97 N04c4861c1c0349faac5b364e60566a7b rdf:first sg:person.01263633521.09
98 rdf:rest Na232c3bd17354697a27267e6a3b87d54
99 N38ba4a8aaee2407c8f1047abed9b87b6 schema:name doi
100 schema:value 10.1007/bf00196562
101 rdf:type schema:PropertyValue
102 N39630db544c0491093897b97ab69c0e4 rdf:first sg:person.01224222341.91
103 rdf:rest rdf:nil
104 N550f55e49de7456caa0532053885bf8d schema:volumeNumber 199
105 rdf:type schema:PublicationVolume
106 N60bd60342eeb4ad5a9cf56684aa943c6 rdf:first sg:person.01262371225.25
107 rdf:rest N04c4861c1c0349faac5b364e60566a7b
108 N627b4b72c8a1433388b1dfd97cd00d41 schema:issueNumber 2
109 rdf:type schema:PublicationIssue
110 Na232c3bd17354697a27267e6a3b87d54 rdf:first sg:person.0627102647.36
111 rdf:rest N39630db544c0491093897b97ab69c0e4
112 Naceaa29b1c104355b5cb793b5ae71360 schema:name Springer Nature - SN SciGraph project
113 rdf:type schema:Organization
114 Ne4395574030548dbab8a9505a7068d15 schema:name dimensions_id
115 schema:value pub.1023431946
116 rdf:type schema:PropertyValue
117 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
118 schema:name Biological Sciences
119 rdf:type schema:DefinedTerm
120 anzsrc-for:0601 schema:inDefinedTermSet anzsrc-for:
121 schema:name Biochemistry and Cell Biology
122 rdf:type schema:DefinedTerm
123 sg:journal.1054035 schema:issn 0032-0935
124 1432-2048
125 schema:name Planta
126 schema:publisher Springer Nature
127 rdf:type schema:Periodical
128 sg:person.01224222341.91 schema:affiliation grid-institutes:grid.1008.9
129 schema:familyName Wetherbee
130 schema:givenName Richard
131 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01224222341.91
132 rdf:type schema:Person
133 sg:person.01262371225.25 schema:affiliation grid-institutes:grid.1008.9
134 schema:familyName Ludwig
135 schema:givenName Martha
136 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01262371225.25
137 rdf:type schema:Person
138 sg:person.01263633521.09 schema:affiliation grid-institutes:grid.1008.9
139 schema:familyName Lind
140 schema:givenName Jan L.
141 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01263633521.09
142 rdf:type schema:Person
143 sg:person.0627102647.36 schema:affiliation grid-institutes:grid.1008.9
144 schema:familyName Miller
145 schema:givenName Elizabeth A.
146 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0627102647.36
147 rdf:type schema:Person
148 sg:pub.10.1007/978-1-4612-5944-2_8 schema:sameAs https://app.dimensions.ai/details/publication/pub.1003560034
149 https://doi.org/10.1007/978-1-4612-5944-2_8
150 rdf:type schema:CreativeWork
151 sg:pub.10.1007/bf00195889 schema:sameAs https://app.dimensions.ai/details/publication/pub.1014420218
152 https://doi.org/10.1007/bf00195889
153 rdf:type schema:CreativeWork
154 sg:pub.10.1007/bf00239985 schema:sameAs https://app.dimensions.ai/details/publication/pub.1050413047
155 https://doi.org/10.1007/bf00239985
156 rdf:type schema:CreativeWork
157 sg:pub.10.1007/bf00348902 schema:sameAs https://app.dimensions.ai/details/publication/pub.1043888630
158 https://doi.org/10.1007/bf00348902
159 rdf:type schema:CreativeWork
160 sg:pub.10.1007/bf01247899 schema:sameAs https://app.dimensions.ai/details/publication/pub.1044629391
161 https://doi.org/10.1007/bf01247899
162 rdf:type schema:CreativeWork
163 sg:pub.10.1007/bf01281167 schema:sameAs https://app.dimensions.ai/details/publication/pub.1052825704
164 https://doi.org/10.1007/bf01281167
165 rdf:type schema:CreativeWork
166 sg:pub.10.1007/bf01666387 schema:sameAs https://app.dimensions.ai/details/publication/pub.1021387866
167 https://doi.org/10.1007/bf01666387
168 rdf:type schema:CreativeWork
169 sg:pub.10.1007/bf01666400 schema:sameAs https://app.dimensions.ai/details/publication/pub.1002593176
170 https://doi.org/10.1007/bf01666400
171 rdf:type schema:CreativeWork
172 sg:pub.10.1038/227680a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1010419937
173 https://doi.org/10.1038/227680a0
174 rdf:type schema:CreativeWork
175 grid-institutes:grid.1008.9 schema:alternateName School of Botany, The University of Melbourne, 3052, Parkville, Victoria, Australia
176 schema:name School of Botany, The University of Melbourne, 3052, Parkville, Victoria, Australia
177 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...