Integration of foreign sequences into the tobacco plastome via polyethylene glycol-mediated protoplast transformation View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1996-06

AUTHORS

Hans-Ulrich Koop, Klaus Steinmüller, Hiltrud Wagner, Christine Rößler, Christian Eibl, Lydia Sacher

ABSTRACT

A new vector, pFaadAII, for transformation of plastids of Nicotiana tabacum L. has been developed. It harbours a chimeric gene consisting of the aadA coding region from Escherichia coli, the 16S rDNA promoter from tobacco combined with a synthetic ribosome-binding site, a 500-bp fragment containing the 3' untranslated transcript region (UTR) of the Chlamydomonas rbcL gene and 3.75-kb (5') and 0.95-kb (3') tobacco plastome sequences allowing for targeting the foreign sequences to the intergenic region between the rpl32 and trnL genes of the tobacco plastome. The vector thus targets foreign sequences to the small single-copy region of the plastome, which has so far not been modified by transformation. Leaf protoplasts of Nicotiana tabacum L. were treated with polyethylene glycol (PEG) in the presence of the vector. The protocol for PEG treatment aiming at plastome transformation was optimized. Cell lines were cultured in the presence of spectinomycin and streptomycin using a novel and efficient protoplast culture and selection system. Regenerants were characterized by polymerase chain reaction (PCR) analysis, Southern hybridization and reciprocal crossing. The transformation procedure is described in detail and parameters influencing its efficiency are presented. Special effort is placed on analyzing suitable selection conditions. Only a proportion of the cell lines with a resistant phenotype could be confirmed by molecular analysis and/or reciprocal crossings to represent plastome transformants. Integration of the plastome specific aadA cassette into the nuclear genome accounted for a fraction of the resistant cell lines. Still, as many as 20-40 plastome transformants can be expected from the treatment of 10(6) protoplasts. Therefore, the improved protocol for PEG-mediated plastome transformation in combination with the new aadA-vector supplies a simple, reproducible and cost-efficient alternative to the biolistic procedure. More... »

PAGES

193-201

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf00196559

DOI

http://dx.doi.org/10.1007/bf00196559

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1027026104

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/8680308


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0604", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Genetics", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Base Sequence", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Blotting, Southern", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "DNA, Plant", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Molecular Sequence Data", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Plants, Toxic", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Plastids", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Polyethylene Glycols", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Polymerase Chain Reaction", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Protoplasts", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Tobacco", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Transformation, Genetic", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Ludwig Maximilian University of Munich", 
          "id": "https://www.grid.ac/institutes/grid.5252.0", 
          "name": [
            "Botanisches Institut der Universit\u00e4t M\u00fcnchen, Menzinger Strasse 67, D-80638, M\u00fcnchen, Germany"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Koop", 
        "givenName": "Hans-Ulrich", 
        "id": "sg:person.01076711412.23", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01076711412.23"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Heinrich Heine University D\u00fcsseldorf", 
          "id": "https://www.grid.ac/institutes/grid.411327.2", 
          "name": [
            "Botanisches Institut, Universit\u00e4t D\u00fcsseldorf, Universit\u00e4tsstrasse 1, D-40225, D\u00fcsseldorf, Germany"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Steinm\u00fcller", 
        "givenName": "Klaus", 
        "id": "sg:person.01061567666.47", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01061567666.47"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Ludwig Maximilian University of Munich", 
          "id": "https://www.grid.ac/institutes/grid.5252.0", 
          "name": [
            "Botanisches Institut der Universit\u00e4t M\u00fcnchen, Menzinger Strasse 67, D-80638, M\u00fcnchen, Germany"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Wagner", 
        "givenName": "Hiltrud", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Ludwig Maximilian University of Munich", 
          "id": "https://www.grid.ac/institutes/grid.5252.0", 
          "name": [
            "Botanisches Institut der Universit\u00e4t M\u00fcnchen, Menzinger Strasse 67, D-80638, M\u00fcnchen, Germany"
          ], 
          "type": "Organization"
        }, 
        "familyName": "R\u00f6\u00dfler", 
        "givenName": "Christine", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Ludwig Maximilian University of Munich", 
          "id": "https://www.grid.ac/institutes/grid.5252.0", 
          "name": [
            "Botanisches Institut der Universit\u00e4t M\u00fcnchen, Menzinger Strasse 67, D-80638, M\u00fcnchen, Germany"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Eibl", 
        "givenName": "Christian", 
        "id": "sg:person.0721331122.72", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0721331122.72"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Ludwig Maximilian University of Munich", 
          "id": "https://www.grid.ac/institutes/grid.5252.0", 
          "name": [
            "Botanisches Institut der Universit\u00e4t M\u00fcnchen, Menzinger Strasse 67, D-80638, M\u00fcnchen, Germany"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Sacher", 
        "givenName": "Lydia", 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.1111/j.1399-3054.1990.tb05887.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1001131700"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.91.5.1969", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1006366479"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1365-313x.1993.00729.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1006818412"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00227316", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1015053158", 
          "https://doi.org/10.1007/bf00227316"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00227316", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1015053158", 
          "https://doi.org/10.1007/bf00227316"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00227316", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1015053158", 
          "https://doi.org/10.1007/bf00227316"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nbt0495-362", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1016061067", 
          "https://doi.org/10.1038/nbt0495-362"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00280200", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1016095007", 
          "https://doi.org/10.1007/bf00280200"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00280200", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1016095007", 
          "https://doi.org/10.1007/bf00280200"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/nar/22.19.3819", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1016205464"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/nar/8.19.4321", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1018245215"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00015814", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1018302490", 
          "https://doi.org/10.1007/bf00015814"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00015814", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1018302490", 
          "https://doi.org/10.1007/bf00015814"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/nar/15.14.5890", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1021342537"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/978-3-642-79247-2_9", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1022730415", 
          "https://doi.org/10.1007/978-3-642-79247-2_9"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1399-3054.1962.tb08052.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1023703429"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00018560", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1027339767", 
          "https://doi.org/10.1007/bf00018560"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00018560", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1027339767", 
          "https://doi.org/10.1007/bf00018560"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0014-4827(68)90403-5", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1027478679"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0378-1119(84)90059-3", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1029504724"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0378-1119(84)90059-3", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1029504724"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.90.3.913", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1029634162"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0378-1119(83)90223-8", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1031489453"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.91.16.7468", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1035703187"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/bies.950060608", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1036818234"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1399-3054.1994.tb06652.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1038932807"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/nar/19.15.4083", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1039706643"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1046/j.1365-313x.1995.07050845.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1047836787"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nbt0193-95", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1048570334", 
          "https://doi.org/10.1038/nbt0193-95"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.91.15.7301", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1051513641"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.87.21.8526", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1051694857"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1126/science.2897716", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1062571083"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/j.1460-2075.1986.tb04464.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1077183847"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/j.1460-2075.1987.tb02730.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1079687101"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/j.1460-2075.1994.tb06784.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1082567686"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/j.1460-2075.1993.tb05692.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1082807468"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "1996-06", 
    "datePublishedReg": "1996-06-01", 
    "description": "A new vector, pFaadAII, for transformation of plastids of Nicotiana tabacum L. has been developed. It harbours a chimeric gene consisting of the aadA coding region from Escherichia coli, the 16S rDNA promoter from tobacco combined with a synthetic ribosome-binding site, a 500-bp fragment containing the 3' untranslated transcript region (UTR) of the Chlamydomonas rbcL gene and 3.75-kb (5') and 0.95-kb (3') tobacco plastome sequences allowing for targeting the foreign sequences to the intergenic region between the rpl32 and trnL genes of the tobacco plastome. The vector thus targets foreign sequences to the small single-copy region of the plastome, which has so far not been modified by transformation. Leaf protoplasts of Nicotiana tabacum L. were treated with polyethylene glycol (PEG) in the presence of the vector. The protocol for PEG treatment aiming at plastome transformation was optimized. Cell lines were cultured in the presence of spectinomycin and streptomycin using a novel and efficient protoplast culture and selection system. Regenerants were characterized by polymerase chain reaction (PCR) analysis, Southern hybridization and reciprocal crossing. The transformation procedure is described in detail and parameters influencing its efficiency are presented. Special effort is placed on analyzing suitable selection conditions. Only a proportion of the cell lines with a resistant phenotype could be confirmed by molecular analysis and/or reciprocal crossings to represent plastome transformants. Integration of the plastome specific aadA cassette into the nuclear genome accounted for a fraction of the resistant cell lines. Still, as many as 20-40 plastome transformants can be expected from the treatment of 10(6) protoplasts. Therefore, the improved protocol for PEG-mediated plastome transformation in combination with the new aadA-vector supplies a simple, reproducible and cost-efficient alternative to the biolistic procedure.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1007/bf00196559", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": false, 
    "isPartOf": [
      {
        "id": "sg:journal.1054035", 
        "issn": [
          "0032-0935", 
          "1432-2048"
        ], 
        "name": "Planta", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "2", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "199"
      }
    ], 
    "name": "Integration of foreign sequences into the tobacco plastome via polyethylene glycol-mediated protoplast transformation", 
    "pagination": "193-201", 
    "productId": [
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1007/bf00196559"
        ]
      }, 
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "80bf411338631241a09a48fca6170e72946b07bcdb19b068a488dbe0c5bbdb48"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1027026104"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "1250576"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "8680308"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1007/bf00196559", 
      "https://app.dimensions.ai/details/publication/pub.1027026104"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-15T08:48", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000374_0000000374/records_119717_00000000.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "http://link.springer.com/10.1007/BF00196559"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/bf00196559'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/bf00196559'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/bf00196559'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/bf00196559'


 

This table displays all metadata directly associated to this object as RDF triples.

245 TRIPLES      21 PREDICATES      70 URIs      32 LITERALS      20 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1007/bf00196559 schema:about N03a00275d55c479f85a15211a264dbbe
2 N3a6aa06a8c094bf9bb14d350ac39746a
3 N3c37b817362243819634a147a1fe8f16
4 N93ef3b254e404f6491de6fc906f539a4
5 N9e98b08451bb4dbc8cbe402124e4684c
6 Nb279f57525564bcb99d382992a9742c6
7 Nb48a470e615d4c7d9853d99c8dd13fb2
8 Ncd8866445e9f4339abd87dce4c5d3033
9 Ne1bc80c90c3f4446ab4d9bddce45b788
10 Nf057fc870ab6436398f9440276e952fc
11 Nf5ade8d63a844cb2b6eb02e1f25f2738
12 anzsrc-for:06
13 anzsrc-for:0604
14 schema:author N26b81f68b6ee42c290f51f98c0121eb6
15 schema:citation sg:pub.10.1007/978-3-642-79247-2_9
16 sg:pub.10.1007/bf00015814
17 sg:pub.10.1007/bf00018560
18 sg:pub.10.1007/bf00227316
19 sg:pub.10.1007/bf00280200
20 sg:pub.10.1038/nbt0193-95
21 sg:pub.10.1038/nbt0495-362
22 https://doi.org/10.1002/bies.950060608
23 https://doi.org/10.1002/j.1460-2075.1986.tb04464.x
24 https://doi.org/10.1002/j.1460-2075.1987.tb02730.x
25 https://doi.org/10.1002/j.1460-2075.1993.tb05692.x
26 https://doi.org/10.1002/j.1460-2075.1994.tb06784.x
27 https://doi.org/10.1016/0014-4827(68)90403-5
28 https://doi.org/10.1016/0378-1119(83)90223-8
29 https://doi.org/10.1016/0378-1119(84)90059-3
30 https://doi.org/10.1046/j.1365-313x.1995.07050845.x
31 https://doi.org/10.1073/pnas.87.21.8526
32 https://doi.org/10.1073/pnas.90.3.913
33 https://doi.org/10.1073/pnas.91.15.7301
34 https://doi.org/10.1073/pnas.91.16.7468
35 https://doi.org/10.1073/pnas.91.5.1969
36 https://doi.org/10.1093/nar/15.14.5890
37 https://doi.org/10.1093/nar/19.15.4083
38 https://doi.org/10.1093/nar/22.19.3819
39 https://doi.org/10.1093/nar/8.19.4321
40 https://doi.org/10.1111/j.1365-313x.1993.00729.x
41 https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
42 https://doi.org/10.1111/j.1399-3054.1990.tb05887.x
43 https://doi.org/10.1111/j.1399-3054.1994.tb06652.x
44 https://doi.org/10.1126/science.2897716
45 schema:datePublished 1996-06
46 schema:datePublishedReg 1996-06-01
47 schema:description A new vector, pFaadAII, for transformation of plastids of Nicotiana tabacum L. has been developed. It harbours a chimeric gene consisting of the aadA coding region from Escherichia coli, the 16S rDNA promoter from tobacco combined with a synthetic ribosome-binding site, a 500-bp fragment containing the 3' untranslated transcript region (UTR) of the Chlamydomonas rbcL gene and 3.75-kb (5') and 0.95-kb (3') tobacco plastome sequences allowing for targeting the foreign sequences to the intergenic region between the rpl32 and trnL genes of the tobacco plastome. The vector thus targets foreign sequences to the small single-copy region of the plastome, which has so far not been modified by transformation. Leaf protoplasts of Nicotiana tabacum L. were treated with polyethylene glycol (PEG) in the presence of the vector. The protocol for PEG treatment aiming at plastome transformation was optimized. Cell lines were cultured in the presence of spectinomycin and streptomycin using a novel and efficient protoplast culture and selection system. Regenerants were characterized by polymerase chain reaction (PCR) analysis, Southern hybridization and reciprocal crossing. The transformation procedure is described in detail and parameters influencing its efficiency are presented. Special effort is placed on analyzing suitable selection conditions. Only a proportion of the cell lines with a resistant phenotype could be confirmed by molecular analysis and/or reciprocal crossings to represent plastome transformants. Integration of the plastome specific aadA cassette into the nuclear genome accounted for a fraction of the resistant cell lines. Still, as many as 20-40 plastome transformants can be expected from the treatment of 10(6) protoplasts. Therefore, the improved protocol for PEG-mediated plastome transformation in combination with the new aadA-vector supplies a simple, reproducible and cost-efficient alternative to the biolistic procedure.
48 schema:genre research_article
49 schema:inLanguage en
50 schema:isAccessibleForFree false
51 schema:isPartOf N04eb12b927e946178d99753b9f3cbb24
52 Nda2797966262474390df2255747f471a
53 sg:journal.1054035
54 schema:name Integration of foreign sequences into the tobacco plastome via polyethylene glycol-mediated protoplast transformation
55 schema:pagination 193-201
56 schema:productId N0e371416f17f4901ae4261677562746e
57 N650c7950a0ef48b5927fccd3a446fbb1
58 N768c39e881b24830900fd023e532faca
59 N7f17718816284d9bb85b449cd639453e
60 Nae854050c6ea4fc7a8a44080afdc2b2f
61 schema:sameAs https://app.dimensions.ai/details/publication/pub.1027026104
62 https://doi.org/10.1007/bf00196559
63 schema:sdDatePublished 2019-04-15T08:48
64 schema:sdLicense https://scigraph.springernature.com/explorer/license/
65 schema:sdPublisher N4b27aa96a9c6403d881942a565c657a6
66 schema:url http://link.springer.com/10.1007/BF00196559
67 sgo:license sg:explorer/license/
68 sgo:sdDataset articles
69 rdf:type schema:ScholarlyArticle
70 N03a00275d55c479f85a15211a264dbbe schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
71 schema:name Polyethylene Glycols
72 rdf:type schema:DefinedTerm
73 N04eb12b927e946178d99753b9f3cbb24 schema:issueNumber 2
74 rdf:type schema:PublicationIssue
75 N07e203e5f03b487b8140eba49e1a7edb rdf:first sg:person.0721331122.72
76 rdf:rest N3ce314da1f484da3b28c2ec544d3bdb6
77 N0e371416f17f4901ae4261677562746e schema:name nlm_unique_id
78 schema:value 1250576
79 rdf:type schema:PropertyValue
80 N0f72dca271b64c1a9b9154e86cb22c0c schema:affiliation https://www.grid.ac/institutes/grid.5252.0
81 schema:familyName Wagner
82 schema:givenName Hiltrud
83 rdf:type schema:Person
84 N26b81f68b6ee42c290f51f98c0121eb6 rdf:first sg:person.01076711412.23
85 rdf:rest Nf800dbb3a8ee441aada9f0b4189af4c9
86 N3a6aa06a8c094bf9bb14d350ac39746a schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
87 schema:name Blotting, Southern
88 rdf:type schema:DefinedTerm
89 N3c37b817362243819634a147a1fe8f16 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
90 schema:name Transformation, Genetic
91 rdf:type schema:DefinedTerm
92 N3ce314da1f484da3b28c2ec544d3bdb6 rdf:first Nb16bb866cf804523b9fe1b24d2434a22
93 rdf:rest rdf:nil
94 N4b27aa96a9c6403d881942a565c657a6 schema:name Springer Nature - SN SciGraph project
95 rdf:type schema:Organization
96 N5d61a87f2e2b411caa58dd38b60d6f9c schema:affiliation https://www.grid.ac/institutes/grid.5252.0
97 schema:familyName Rößler
98 schema:givenName Christine
99 rdf:type schema:Person
100 N650c7950a0ef48b5927fccd3a446fbb1 schema:name pubmed_id
101 schema:value 8680308
102 rdf:type schema:PropertyValue
103 N6a774c34f3474e518168f981fa7de9d7 rdf:first N5d61a87f2e2b411caa58dd38b60d6f9c
104 rdf:rest N07e203e5f03b487b8140eba49e1a7edb
105 N768c39e881b24830900fd023e532faca schema:name dimensions_id
106 schema:value pub.1027026104
107 rdf:type schema:PropertyValue
108 N7f17718816284d9bb85b449cd639453e schema:name readcube_id
109 schema:value 80bf411338631241a09a48fca6170e72946b07bcdb19b068a488dbe0c5bbdb48
110 rdf:type schema:PropertyValue
111 N93ef3b254e404f6491de6fc906f539a4 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
112 schema:name Plants, Toxic
113 rdf:type schema:DefinedTerm
114 N9e98b08451bb4dbc8cbe402124e4684c schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
115 schema:name Protoplasts
116 rdf:type schema:DefinedTerm
117 Nae854050c6ea4fc7a8a44080afdc2b2f schema:name doi
118 schema:value 10.1007/bf00196559
119 rdf:type schema:PropertyValue
120 Nb16bb866cf804523b9fe1b24d2434a22 schema:affiliation https://www.grid.ac/institutes/grid.5252.0
121 schema:familyName Sacher
122 schema:givenName Lydia
123 rdf:type schema:Person
124 Nb279f57525564bcb99d382992a9742c6 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
125 schema:name Molecular Sequence Data
126 rdf:type schema:DefinedTerm
127 Nb48a470e615d4c7d9853d99c8dd13fb2 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
128 schema:name Plastids
129 rdf:type schema:DefinedTerm
130 Nbc0b51b37915405380a9cd821a523d44 rdf:first N0f72dca271b64c1a9b9154e86cb22c0c
131 rdf:rest N6a774c34f3474e518168f981fa7de9d7
132 Ncd8866445e9f4339abd87dce4c5d3033 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
133 schema:name DNA, Plant
134 rdf:type schema:DefinedTerm
135 Nda2797966262474390df2255747f471a schema:volumeNumber 199
136 rdf:type schema:PublicationVolume
137 Ne1bc80c90c3f4446ab4d9bddce45b788 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
138 schema:name Base Sequence
139 rdf:type schema:DefinedTerm
140 Nf057fc870ab6436398f9440276e952fc schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
141 schema:name Polymerase Chain Reaction
142 rdf:type schema:DefinedTerm
143 Nf5ade8d63a844cb2b6eb02e1f25f2738 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
144 schema:name Tobacco
145 rdf:type schema:DefinedTerm
146 Nf800dbb3a8ee441aada9f0b4189af4c9 rdf:first sg:person.01061567666.47
147 rdf:rest Nbc0b51b37915405380a9cd821a523d44
148 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
149 schema:name Biological Sciences
150 rdf:type schema:DefinedTerm
151 anzsrc-for:0604 schema:inDefinedTermSet anzsrc-for:
152 schema:name Genetics
153 rdf:type schema:DefinedTerm
154 sg:journal.1054035 schema:issn 0032-0935
155 1432-2048
156 schema:name Planta
157 rdf:type schema:Periodical
158 sg:person.01061567666.47 schema:affiliation https://www.grid.ac/institutes/grid.411327.2
159 schema:familyName Steinmüller
160 schema:givenName Klaus
161 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01061567666.47
162 rdf:type schema:Person
163 sg:person.01076711412.23 schema:affiliation https://www.grid.ac/institutes/grid.5252.0
164 schema:familyName Koop
165 schema:givenName Hans-Ulrich
166 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01076711412.23
167 rdf:type schema:Person
168 sg:person.0721331122.72 schema:affiliation https://www.grid.ac/institutes/grid.5252.0
169 schema:familyName Eibl
170 schema:givenName Christian
171 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0721331122.72
172 rdf:type schema:Person
173 sg:pub.10.1007/978-3-642-79247-2_9 schema:sameAs https://app.dimensions.ai/details/publication/pub.1022730415
174 https://doi.org/10.1007/978-3-642-79247-2_9
175 rdf:type schema:CreativeWork
176 sg:pub.10.1007/bf00015814 schema:sameAs https://app.dimensions.ai/details/publication/pub.1018302490
177 https://doi.org/10.1007/bf00015814
178 rdf:type schema:CreativeWork
179 sg:pub.10.1007/bf00018560 schema:sameAs https://app.dimensions.ai/details/publication/pub.1027339767
180 https://doi.org/10.1007/bf00018560
181 rdf:type schema:CreativeWork
182 sg:pub.10.1007/bf00227316 schema:sameAs https://app.dimensions.ai/details/publication/pub.1015053158
183 https://doi.org/10.1007/bf00227316
184 rdf:type schema:CreativeWork
185 sg:pub.10.1007/bf00280200 schema:sameAs https://app.dimensions.ai/details/publication/pub.1016095007
186 https://doi.org/10.1007/bf00280200
187 rdf:type schema:CreativeWork
188 sg:pub.10.1038/nbt0193-95 schema:sameAs https://app.dimensions.ai/details/publication/pub.1048570334
189 https://doi.org/10.1038/nbt0193-95
190 rdf:type schema:CreativeWork
191 sg:pub.10.1038/nbt0495-362 schema:sameAs https://app.dimensions.ai/details/publication/pub.1016061067
192 https://doi.org/10.1038/nbt0495-362
193 rdf:type schema:CreativeWork
194 https://doi.org/10.1002/bies.950060608 schema:sameAs https://app.dimensions.ai/details/publication/pub.1036818234
195 rdf:type schema:CreativeWork
196 https://doi.org/10.1002/j.1460-2075.1986.tb04464.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1077183847
197 rdf:type schema:CreativeWork
198 https://doi.org/10.1002/j.1460-2075.1987.tb02730.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1079687101
199 rdf:type schema:CreativeWork
200 https://doi.org/10.1002/j.1460-2075.1993.tb05692.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1082807468
201 rdf:type schema:CreativeWork
202 https://doi.org/10.1002/j.1460-2075.1994.tb06784.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1082567686
203 rdf:type schema:CreativeWork
204 https://doi.org/10.1016/0014-4827(68)90403-5 schema:sameAs https://app.dimensions.ai/details/publication/pub.1027478679
205 rdf:type schema:CreativeWork
206 https://doi.org/10.1016/0378-1119(83)90223-8 schema:sameAs https://app.dimensions.ai/details/publication/pub.1031489453
207 rdf:type schema:CreativeWork
208 https://doi.org/10.1016/0378-1119(84)90059-3 schema:sameAs https://app.dimensions.ai/details/publication/pub.1029504724
209 rdf:type schema:CreativeWork
210 https://doi.org/10.1046/j.1365-313x.1995.07050845.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1047836787
211 rdf:type schema:CreativeWork
212 https://doi.org/10.1073/pnas.87.21.8526 schema:sameAs https://app.dimensions.ai/details/publication/pub.1051694857
213 rdf:type schema:CreativeWork
214 https://doi.org/10.1073/pnas.90.3.913 schema:sameAs https://app.dimensions.ai/details/publication/pub.1029634162
215 rdf:type schema:CreativeWork
216 https://doi.org/10.1073/pnas.91.15.7301 schema:sameAs https://app.dimensions.ai/details/publication/pub.1051513641
217 rdf:type schema:CreativeWork
218 https://doi.org/10.1073/pnas.91.16.7468 schema:sameAs https://app.dimensions.ai/details/publication/pub.1035703187
219 rdf:type schema:CreativeWork
220 https://doi.org/10.1073/pnas.91.5.1969 schema:sameAs https://app.dimensions.ai/details/publication/pub.1006366479
221 rdf:type schema:CreativeWork
222 https://doi.org/10.1093/nar/15.14.5890 schema:sameAs https://app.dimensions.ai/details/publication/pub.1021342537
223 rdf:type schema:CreativeWork
224 https://doi.org/10.1093/nar/19.15.4083 schema:sameAs https://app.dimensions.ai/details/publication/pub.1039706643
225 rdf:type schema:CreativeWork
226 https://doi.org/10.1093/nar/22.19.3819 schema:sameAs https://app.dimensions.ai/details/publication/pub.1016205464
227 rdf:type schema:CreativeWork
228 https://doi.org/10.1093/nar/8.19.4321 schema:sameAs https://app.dimensions.ai/details/publication/pub.1018245215
229 rdf:type schema:CreativeWork
230 https://doi.org/10.1111/j.1365-313x.1993.00729.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1006818412
231 rdf:type schema:CreativeWork
232 https://doi.org/10.1111/j.1399-3054.1962.tb08052.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1023703429
233 rdf:type schema:CreativeWork
234 https://doi.org/10.1111/j.1399-3054.1990.tb05887.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1001131700
235 rdf:type schema:CreativeWork
236 https://doi.org/10.1111/j.1399-3054.1994.tb06652.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1038932807
237 rdf:type schema:CreativeWork
238 https://doi.org/10.1126/science.2897716 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062571083
239 rdf:type schema:CreativeWork
240 https://www.grid.ac/institutes/grid.411327.2 schema:alternateName Heinrich Heine University Düsseldorf
241 schema:name Botanisches Institut, Universität Düsseldorf, Universitätsstrasse 1, D-40225, Düsseldorf, Germany
242 rdf:type schema:Organization
243 https://www.grid.ac/institutes/grid.5252.0 schema:alternateName Ludwig Maximilian University of Munich
244 schema:name Botanisches Institut der Universität München, Menzinger Strasse 67, D-80638, München, Germany
245 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...