Ontology type: schema:ScholarlyArticle
1996-06
AUTHORSHans-Ulrich Koop, Klaus Steinmüller, Hiltrud Wagner, Christine Rößler, Christian Eibl, Lydia Sacher
ABSTRACTA new vector, pFaadAII, for transformation of plastids of Nicotiana tabacum L. has been developed. It harbours a chimeric gene consisting of the aadA coding region from Escherichia coli, the 16S rDNA promoter from tobacco combined with a synthetic ribosome-binding site, a 500-bp fragment containing the 3' untranslated transcript region (UTR) of the Chlamydomonas rbcL gene and 3.75-kb (5') and 0.95-kb (3') tobacco plastome sequences allowing for targeting the foreign sequences to the intergenic region between the rpl32 and trnL genes of the tobacco plastome. The vector thus targets foreign sequences to the small single-copy region of the plastome, which has so far not been modified by transformation. Leaf protoplasts of Nicotiana tabacum L. were treated with polyethylene glycol (PEG) in the presence of the vector. The protocol for PEG treatment aiming at plastome transformation was optimized. Cell lines were cultured in the presence of spectinomycin and streptomycin using a novel and efficient protoplast culture and selection system. Regenerants were characterized by polymerase chain reaction (PCR) analysis, Southern hybridization and reciprocal crossing. The transformation procedure is described in detail and parameters influencing its efficiency are presented. Special effort is placed on analyzing suitable selection conditions. Only a proportion of the cell lines with a resistant phenotype could be confirmed by molecular analysis and/or reciprocal crossings to represent plastome transformants. Integration of the plastome specific aadA cassette into the nuclear genome accounted for a fraction of the resistant cell lines. Still, as many as 20-40 plastome transformants can be expected from the treatment of 10(6) protoplasts. Therefore, the improved protocol for PEG-mediated plastome transformation in combination with the new aadA-vector supplies a simple, reproducible and cost-efficient alternative to the biolistic procedure. More... »
PAGES193-201
http://scigraph.springernature.com/pub.10.1007/bf00196559
DOIhttp://dx.doi.org/10.1007/bf00196559
DIMENSIONShttps://app.dimensions.ai/details/publication/pub.1027026104
PUBMEDhttps://www.ncbi.nlm.nih.gov/pubmed/8680308
JSON-LD is the canonical representation for SciGraph data.
TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT
[
{
"@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json",
"about": [
{
"id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0604",
"inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/",
"name": "Genetics",
"type": "DefinedTerm"
},
{
"id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06",
"inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/",
"name": "Biological Sciences",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Base Sequence",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Blotting, Southern",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "DNA, Plant",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Molecular Sequence Data",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Plants, Toxic",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Plastids",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Polyethylene Glycols",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Polymerase Chain Reaction",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Protoplasts",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Tobacco",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Transformation, Genetic",
"type": "DefinedTerm"
}
],
"author": [
{
"affiliation": {
"alternateName": "Ludwig Maximilian University of Munich",
"id": "https://www.grid.ac/institutes/grid.5252.0",
"name": [
"Botanisches Institut der Universit\u00e4t M\u00fcnchen, Menzinger Strasse 67, D-80638, M\u00fcnchen, Germany"
],
"type": "Organization"
},
"familyName": "Koop",
"givenName": "Hans-Ulrich",
"id": "sg:person.01076711412.23",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01076711412.23"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Heinrich Heine University D\u00fcsseldorf",
"id": "https://www.grid.ac/institutes/grid.411327.2",
"name": [
"Botanisches Institut, Universit\u00e4t D\u00fcsseldorf, Universit\u00e4tsstrasse 1, D-40225, D\u00fcsseldorf, Germany"
],
"type": "Organization"
},
"familyName": "Steinm\u00fcller",
"givenName": "Klaus",
"id": "sg:person.01061567666.47",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01061567666.47"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Ludwig Maximilian University of Munich",
"id": "https://www.grid.ac/institutes/grid.5252.0",
"name": [
"Botanisches Institut der Universit\u00e4t M\u00fcnchen, Menzinger Strasse 67, D-80638, M\u00fcnchen, Germany"
],
"type": "Organization"
},
"familyName": "Wagner",
"givenName": "Hiltrud",
"type": "Person"
},
{
"affiliation": {
"alternateName": "Ludwig Maximilian University of Munich",
"id": "https://www.grid.ac/institutes/grid.5252.0",
"name": [
"Botanisches Institut der Universit\u00e4t M\u00fcnchen, Menzinger Strasse 67, D-80638, M\u00fcnchen, Germany"
],
"type": "Organization"
},
"familyName": "R\u00f6\u00dfler",
"givenName": "Christine",
"type": "Person"
},
{
"affiliation": {
"alternateName": "Ludwig Maximilian University of Munich",
"id": "https://www.grid.ac/institutes/grid.5252.0",
"name": [
"Botanisches Institut der Universit\u00e4t M\u00fcnchen, Menzinger Strasse 67, D-80638, M\u00fcnchen, Germany"
],
"type": "Organization"
},
"familyName": "Eibl",
"givenName": "Christian",
"id": "sg:person.0721331122.72",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0721331122.72"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Ludwig Maximilian University of Munich",
"id": "https://www.grid.ac/institutes/grid.5252.0",
"name": [
"Botanisches Institut der Universit\u00e4t M\u00fcnchen, Menzinger Strasse 67, D-80638, M\u00fcnchen, Germany"
],
"type": "Organization"
},
"familyName": "Sacher",
"givenName": "Lydia",
"type": "Person"
}
],
"citation": [
{
"id": "https://doi.org/10.1111/j.1399-3054.1990.tb05887.x",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1001131700"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1073/pnas.91.5.1969",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1006366479"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1111/j.1365-313x.1993.00729.x",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1006818412"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/bf00227316",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1015053158",
"https://doi.org/10.1007/bf00227316"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/bf00227316",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1015053158",
"https://doi.org/10.1007/bf00227316"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/bf00227316",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1015053158",
"https://doi.org/10.1007/bf00227316"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/nbt0495-362",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1016061067",
"https://doi.org/10.1038/nbt0495-362"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/bf00280200",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1016095007",
"https://doi.org/10.1007/bf00280200"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/bf00280200",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1016095007",
"https://doi.org/10.1007/bf00280200"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1093/nar/22.19.3819",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1016205464"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1093/nar/8.19.4321",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1018245215"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/bf00015814",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1018302490",
"https://doi.org/10.1007/bf00015814"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/bf00015814",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1018302490",
"https://doi.org/10.1007/bf00015814"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1093/nar/15.14.5890",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1021342537"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/978-3-642-79247-2_9",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1022730415",
"https://doi.org/10.1007/978-3-642-79247-2_9"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1111/j.1399-3054.1962.tb08052.x",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1023703429"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/bf00018560",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1027339767",
"https://doi.org/10.1007/bf00018560"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/bf00018560",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1027339767",
"https://doi.org/10.1007/bf00018560"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/0014-4827(68)90403-5",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1027478679"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/0378-1119(84)90059-3",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1029504724"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/0378-1119(84)90059-3",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1029504724"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1073/pnas.90.3.913",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1029634162"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/0378-1119(83)90223-8",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1031489453"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1073/pnas.91.16.7468",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1035703187"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1002/bies.950060608",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1036818234"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1111/j.1399-3054.1994.tb06652.x",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1038932807"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1093/nar/19.15.4083",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1039706643"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1046/j.1365-313x.1995.07050845.x",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1047836787"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/nbt0193-95",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1048570334",
"https://doi.org/10.1038/nbt0193-95"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1073/pnas.91.15.7301",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1051513641"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1073/pnas.87.21.8526",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1051694857"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1126/science.2897716",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1062571083"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1002/j.1460-2075.1986.tb04464.x",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1077183847"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1002/j.1460-2075.1987.tb02730.x",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1079687101"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1002/j.1460-2075.1994.tb06784.x",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1082567686"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1002/j.1460-2075.1993.tb05692.x",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1082807468"
],
"type": "CreativeWork"
}
],
"datePublished": "1996-06",
"datePublishedReg": "1996-06-01",
"description": "A new vector, pFaadAII, for transformation of plastids of Nicotiana tabacum L. has been developed. It harbours a chimeric gene consisting of the aadA coding region from Escherichia coli, the 16S rDNA promoter from tobacco combined with a synthetic ribosome-binding site, a 500-bp fragment containing the 3' untranslated transcript region (UTR) of the Chlamydomonas rbcL gene and 3.75-kb (5') and 0.95-kb (3') tobacco plastome sequences allowing for targeting the foreign sequences to the intergenic region between the rpl32 and trnL genes of the tobacco plastome. The vector thus targets foreign sequences to the small single-copy region of the plastome, which has so far not been modified by transformation. Leaf protoplasts of Nicotiana tabacum L. were treated with polyethylene glycol (PEG) in the presence of the vector. The protocol for PEG treatment aiming at plastome transformation was optimized. Cell lines were cultured in the presence of spectinomycin and streptomycin using a novel and efficient protoplast culture and selection system. Regenerants were characterized by polymerase chain reaction (PCR) analysis, Southern hybridization and reciprocal crossing. The transformation procedure is described in detail and parameters influencing its efficiency are presented. Special effort is placed on analyzing suitable selection conditions. Only a proportion of the cell lines with a resistant phenotype could be confirmed by molecular analysis and/or reciprocal crossings to represent plastome transformants. Integration of the plastome specific aadA cassette into the nuclear genome accounted for a fraction of the resistant cell lines. Still, as many as 20-40 plastome transformants can be expected from the treatment of 10(6) protoplasts. Therefore, the improved protocol for PEG-mediated plastome transformation in combination with the new aadA-vector supplies a simple, reproducible and cost-efficient alternative to the biolistic procedure.",
"genre": "research_article",
"id": "sg:pub.10.1007/bf00196559",
"inLanguage": [
"en"
],
"isAccessibleForFree": false,
"isPartOf": [
{
"id": "sg:journal.1054035",
"issn": [
"0032-0935",
"1432-2048"
],
"name": "Planta",
"type": "Periodical"
},
{
"issueNumber": "2",
"type": "PublicationIssue"
},
{
"type": "PublicationVolume",
"volumeNumber": "199"
}
],
"name": "Integration of foreign sequences into the tobacco plastome via polyethylene glycol-mediated protoplast transformation",
"pagination": "193-201",
"productId": [
{
"name": "doi",
"type": "PropertyValue",
"value": [
"10.1007/bf00196559"
]
},
{
"name": "readcube_id",
"type": "PropertyValue",
"value": [
"80bf411338631241a09a48fca6170e72946b07bcdb19b068a488dbe0c5bbdb48"
]
},
{
"name": "dimensions_id",
"type": "PropertyValue",
"value": [
"pub.1027026104"
]
},
{
"name": "nlm_unique_id",
"type": "PropertyValue",
"value": [
"1250576"
]
},
{
"name": "pubmed_id",
"type": "PropertyValue",
"value": [
"8680308"
]
}
],
"sameAs": [
"https://doi.org/10.1007/bf00196559",
"https://app.dimensions.ai/details/publication/pub.1027026104"
],
"sdDataset": "articles",
"sdDatePublished": "2019-04-15T08:48",
"sdLicense": "https://scigraph.springernature.com/explorer/license/",
"sdPublisher": {
"name": "Springer Nature - SN SciGraph project",
"type": "Organization"
},
"sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000374_0000000374/records_119717_00000000.jsonl",
"type": "ScholarlyArticle",
"url": "http://link.springer.com/10.1007/BF00196559"
}
]Download the RDF metadata as: json-ld nt turtle xml License info
JSON-LD is a popular format for linked data which is fully compatible with JSON.
curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/bf00196559'
N-Triples is a line-based linked data format ideal for batch operations.
curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/bf00196559'
Turtle is a human-readable linked data format.
curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/bf00196559'
RDF/XML is a standard XML format for linked data.
curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/bf00196559'
This table displays all metadata directly associated to this object as RDF triples.
245 TRIPLES
21 PREDICATES
70 URIs
32 LITERALS
20 BLANK NODES