Video microscopic observations of living, isolated embryo sacs of Nicotiana and their component cells View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1992-04

AUTHORS

B.-Q. Huang, E. S. Pierson, S. D. Russell, A. Tiezzi, M. Cresti

ABSTRACT

Living embryo sacs and megagametophytic cells of Nicotiana alata and Nicotiana tabacum were obtained using enzymatic maceration and microdissection. The yields of isolated embryo sacs, egg apparatus and central cells were up to 35%, 40% and 35%, respectively. Vectorial movement of organelles and undulations of tubular structures, presumably endoplasmic reticulum, were observed in eggs, synergids and central cells using video-enhanced microscopy. Despite evident viability using the fluorochromatic reaction, the egg displays much less organelle movement and therefore appears to be quiescent. The large vacuole of the central cell is traversed by mobile strands of cytoplasm through which organelles migrate. A polygonal network is located at the periphery of the central cell, which may contribute to anchorage of the cell with the embryo-sac wall. The observation of organelle movement provides direct evidence of the condition of the cell and may be a useful approach for assessing cell vigor. More... »

PAGES

156-162

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf00194876

DOI

http://dx.doi.org/10.1007/bf00194876

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1026153077


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