Plant regeneration of grapevine (Vitis sp.) protoplasts isolated from embryogenic tissue View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1995-12

AUTHORS

Götz Reustle, Margit Harst, Gerhardt Alleweldt

ABSTRACT

Protoplasts with high embryogenic competence could be isolated from leaf-disk-derived embryos and embryoids of Vitis sp. cv. Seyval blanc. After a 4-week induction treatment in NN-69 medium supplemented with 4.0mg/l naphthoxyacetic acid (NOA) and 0.9mg/l thidiazuron (TDZ) and subsequent subcultivation in hormone-free medium, 38.5% of the developed microcalluses showed somatic embryogenesis. In contrast, only few formed somatic embryos after induction in CPW-13 medium with either 1.0mg/l 2,4-dichlorophenoxyacetic acid and 0.5mg/l benzylaminopurine treatment (13.8%) or NOA/TDZ treatment (1.4%). Up to 30% of these embryos germinated and about half of them regenerated into typical in vitro grapevines when transferred onto LS-medium in culture tubes. More... »

PAGES

238-241

References to SciGraph publications

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf00193727

DOI

http://dx.doi.org/10.1007/bf00193727

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1019835255

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/24185783


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