Thidiazuron-induced plant regeneration from protoplasts of Vicia faba cv. Mythos View Full Text


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Article Info

DATE

1995-12

AUTHORS

Mechthild Tegeder, Dorothea Gebhardt, Otto Schieder, Thomas Pickardt

ABSTRACT

Protoplasts of 10 cultivars of V. faba were isolated from etiolated shoot-tips and tested for their regeneration capacity. After purification, protoplasts were embedded in sodium alginate and cultivated in the medium of Kao and Michayluk (1975) containing 0.5 mg·1(-1) of each 2,4-dichlorophenoxyacetic acid, naphthylacetic acid and 6-benzylaminopurine. Depending on cultivar, division frequencies of up to 40% were obtained. Six weeks after embedding, protoplast-derived calluses were transferred to Gelrite-solidified media with different combinations of growth regulators. A two step protocol (auxin high/low) was tested for its ability to induce somatic embryogenesis. The formation of globular structures was observed, but no embryo formation could be achieved. In contrast, cultivation of protocalluses on medium supplemented with thidiazuron resulted in shoot development in cultivar Mythos. To generate mature plants, the shoots were grafted onto young seedlings. In order to optimize the in vitro-conditions, different concentrations of thidiazuron alone or in combination with naphthylacetic acid were tested, showing that an increase of thidiazuron and the addition of naphthylacetic acid positively affects both the viability of protocalluses and the regeneration frequency. More... »

PAGES

164-169

References to SciGraph publications

  • 1993-05. Thidiazuron: a potent cytokinin for woody plant tissue culture in PLANT CELL, TISSUE AND ORGAN CULTURE (PCTOC)
  • 1988-08. Plant regeneration from protoplasts of soybean (Glycine max L.) in PLANT CELL REPORTS
  • 1975-01. Nutritional requirements for growth of Vicia hajastana cells and protoplasts at a very low population density in liquid media in PLANTA
  • 1994-01. A chimeric gene encoding the methionine-rich 2S albumin of the Brazil nut (Bertlrolletia excelsa H.B.K.) is stably expressed and inherited in transgenic grain legumes in MOLECULAR GENETICS AND GENOMICS
  • 1988-06. Improved protoplast culture and regeneration of shoots in pea (Pisum sativum L.) in PLANT CELL REPORTS
  • 1991-05. Plantlet regeneration from immature cotyledon protoplasts of soybean (Glycine max L.) in PLANT CELL REPORTS
  • 1991-06. Regeneration of transformed shoots from electroporated soybean (Glycine max (L.) Merr.) protoplasts in PLANT CELL REPORTS
  • 1989-02. Plant regeneration via somatic embryogenesis inVicia narbonensis in PROTOPLASMA
  • 1993-01. Somatic embryogenesis in Nicotiana tabacum L.: induction by thidiazuron of direct embryo differentiation from cultured leaf discs in PLANT CELL REPORTS
  • 1985-01. Protoplast culture and plant regeneration in Glycine canescens F.J. Herm in PLANT CELL, TISSUE AND ORGAN CULTURE (PCTOC)
  • 1992-02. Regeneration in Phaseolus vulgaris L.: High-frequency induction of direct shoot formation in intact seedlings by N6-benzylaminopurine and thidiazuron in PLANTA
  • 1989-07. Plant regeneration from seedling explants and cotyledon protoplasts ofGlycine argyrea tind in IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY
  • 1989-10. Plant regeneration from pea protoplasts via somatic embyogenesis in PLANT CELL REPORTS
  • 1983-10. Agarose plating and a bead type culture technique enable and stimulate development of protoplast-derived colonies in a number of plant species in PLANT CELL REPORTS
  • 1988-07. Regeneration of fertile plants from protoplasts of different Arabidopsis thaliana genotypes in MOLECULAR GENETICS AND GENOMICS
  • 1993-07. Plant regeneration from leaf protoplasts of apple in PLANT CELL, TISSUE AND ORGAN CULTURE (PCTOC)
  • 1987-07. Stable transformation of moth bean Vigna aconitifolia via direct gene transfer in PLANT CELL REPORTS
  • 1992-06. Regeneration of fertile plants from protoplasts of soybean (Glycine max L. Merr.): genotypic differences in culture response in PLANT CELL REPORTS
  • 1989-02. Plant regeneration of wildGlycine species from suspension culture-derived protoplasts in PLANT CELL REPORTS
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/bf00193712

    DOI

    http://dx.doi.org/10.1007/bf00193712

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1006569994

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/24185768


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