Ontology type: schema:ScholarlyArticle
1990-07
AUTHORSJulie Burns, Georg Melmer, Johanna M. Rommens, John R. Riordan, Manuel Buchwald
ABSTRACTThis paper describes an approach that can be used to identify specifically expressed coding sequences in defined regions of genomic DNA. We developed this method to identify expressed sequences from chromosome 7 located at or near the cystic fibrosis (CF) locus. Radioactively labelled single-stranded cDNAs derived from sweat gland epithelial cells and from fibroblasts were used to screen a genomic library constructed from flow-sorted chromosomes. Differential screening of phage lifts with these two probes yielded 36 different DNA segments. By using somatic cell hybrids containing different portions of chromosome 7, four of the clones were mapped to the 7q31 region in which the CF locus is located. These four clones and two others that gave strong differential epithelial signals but that were not within 7q31 were studied further. Restriction fragment length polymorphisms (RFLPs) were identified for two of the DNA segments within 7q31 and used for linkage analysis using a panel of CF families. One DNA segment was assigned to a location centromeric to the met locus. The other marker did not show recombination with CF but was subsequently excluded from the CF region by physical mapping. Three of the six DNA segments were found to hybridize to various RNAs using the Northern technique and therefore contain portions of genes. One of the clones showed strong differential expression when epithelial tissues were compared to fibroblasts and may represent an epithelium-specific gene. More... »
PAGES151-156
http://scigraph.springernature.com/pub.10.1007/bf00193187
DOIhttp://dx.doi.org/10.1007/bf00193187
DIMENSIONShttps://app.dimensions.ai/details/publication/pub.1031354867
PUBMEDhttps://www.ncbi.nlm.nih.gov/pubmed/1973401
JSON-LD is the canonical representation for SciGraph data.
TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT
[
{
"@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json",
"about": [
{
"id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0604",
"inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/",
"name": "Genetics",
"type": "DefinedTerm"
},
{
"id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06",
"inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/",
"name": "Biological Sciences",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Autoradiography",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Blotting, Northern",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Chromosome Mapping",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Chromosomes, Human, Pair 7",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Cystic Fibrosis",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "DNA Probes",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Epithelium",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Genetic Linkage",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Genome, Human",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Humans",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Nucleic Acid Hybridization",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Polymorphism, Restriction Fragment Length",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Sweat Glands",
"type": "DefinedTerm"
}
],
"author": [
{
"affiliation": {
"alternateName": "University of Toronto",
"id": "https://www.grid.ac/institutes/grid.17063.33",
"name": [
"Research Institute, The Hospital for Sick Children, University of Toronto, Toronto, Ontario, Canada"
],
"type": "Organization"
},
"familyName": "Burns",
"givenName": "Julie",
"id": "sg:person.01043600625.49",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01043600625.49"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "University of Toronto",
"id": "https://www.grid.ac/institutes/grid.17063.33",
"name": [
"Research Institute, The Hospital for Sick Children, University of Toronto, Toronto, Ontario, Canada"
],
"type": "Organization"
},
"familyName": "Melmer",
"givenName": "Georg",
"id": "sg:person.01111714025.15",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01111714025.15"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "University of Toronto",
"id": "https://www.grid.ac/institutes/grid.17063.33",
"name": [
"Research Institute, The Hospital for Sick Children, University of Toronto, Toronto, Ontario, Canada"
],
"type": "Organization"
},
"familyName": "Rommens",
"givenName": "Johanna M.",
"id": "sg:person.01355172356.46",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01355172356.46"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "University of Toronto",
"id": "https://www.grid.ac/institutes/grid.17063.33",
"name": [
"Department of Biochemistry, University of Toronto, Toronto, Ontario, Canada",
"Department of Clinical Biochemistry, University of Toronto, Toronto, Ontario, Canada"
],
"type": "Organization"
},
"familyName": "Riordan",
"givenName": "John R.",
"id": "sg:person.01110637425.01",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01110637425.01"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "University of Toronto",
"id": "https://www.grid.ac/institutes/grid.17063.33",
"name": [
"Research Institute, The Hospital for Sick Children, University of Toronto, Toronto, Ontario, Canada",
"Department of Medical Genetics, University of Toronto, Toronto, Ontario, Canada",
"Department of Medical Biophysics, University of Toronto, Toronto, Ontario, Canada"
],
"type": "Organization"
},
"familyName": "Buchwald",
"givenName": "Manuel",
"id": "sg:person.0575412723.38",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0575412723.38"
],
"type": "Person"
}
],
"citation": [
{
"id": "https://doi.org/10.1128/mcb.3.11.2076",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1002276360"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/322467a0",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1003280070",
"https://doi.org/10.1038/322467a0"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/bf01535047",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1009340325",
"https://doi.org/10.1007/bf01535047"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/301421a0",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1012666516",
"https://doi.org/10.1038/301421a0"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/0003-2697(83)90418-9",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1012973560"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/0014-5793(88)80189-3",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1013429407"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/0092-8674(79)90020-5",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1013562585"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1159/000132684",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1019013534"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/bf02620892",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1025736530",
"https://doi.org/10.1007/bf02620892"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/bf02620892",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1025736530",
"https://doi.org/10.1007/bf02620892"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/978-1-4612-0475-6",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1026679020",
"https://doi.org/10.1007/978-1-4612-0475-6"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/978-1-4612-0475-6",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1026679020",
"https://doi.org/10.1007/978-1-4612-0475-6"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1073/pnas.69.6.1408",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1029159199"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1093/nar/13.6.1905",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1032212651"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/330752a0",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1037827038",
"https://doi.org/10.1038/330752a0"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1098/rstb.1988.0048",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1042908142"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/s0009-9120(84)80147-2",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1043128508"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/0378-1119(86)90372-0",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1044760967"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/0378-1119(86)90372-0",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1044760967"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/0378-1119(87)90087-4",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1045237969"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/0378-1119(87)90087-4",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1045237969"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/331358a0",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1047701223",
"https://doi.org/10.1038/331358a0"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/0378-1119(85)90216-1",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1053380011"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/0378-1119(85)90216-1",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1053380011"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1021/bi00591a005",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1055181913"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1101/sqb.1986.051.01.019",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1060405769"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1126/science.2425436",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1062537172"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1126/science.2464852",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1062538894"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1126/science.2475911",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1062539396"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1126/science.2570460",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1062544667"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1126/science.2772657",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1062558148"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1126/science.2997931",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1062580093"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1126/science.6308769",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1062636695"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1126/science.6688681",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1062639387"
],
"type": "CreativeWork"
},
{
"id": "https://app.dimensions.ai/details/publication/pub.1078850311",
"type": "CreativeWork"
},
{
"id": "https://app.dimensions.ai/details/publication/pub.1079447021",
"type": "CreativeWork"
},
{
"id": "https://app.dimensions.ai/details/publication/pub.1079770044",
"type": "CreativeWork"
},
{
"id": "https://app.dimensions.ai/details/publication/pub.1080114119",
"type": "CreativeWork"
},
{
"id": "https://app.dimensions.ai/details/publication/pub.1080334869",
"type": "CreativeWork"
}
],
"datePublished": "1990-07",
"datePublishedReg": "1990-07-01",
"description": "This paper describes an approach that can be used to identify specifically expressed coding sequences in defined regions of genomic DNA. We developed this method to identify expressed sequences from chromosome 7 located at or near the cystic fibrosis (CF) locus. Radioactively labelled single-stranded cDNAs derived from sweat gland epithelial cells and from fibroblasts were used to screen a genomic library constructed from flow-sorted chromosomes. Differential screening of phage lifts with these two probes yielded 36 different DNA segments. By using somatic cell hybrids containing different portions of chromosome 7, four of the clones were mapped to the 7q31 region in which the CF locus is located. These four clones and two others that gave strong differential epithelial signals but that were not within 7q31 were studied further. Restriction fragment length polymorphisms (RFLPs) were identified for two of the DNA segments within 7q31 and used for linkage analysis using a panel of CF families. One DNA segment was assigned to a location centromeric to the met locus. The other marker did not show recombination with CF but was subsequently excluded from the CF region by physical mapping. Three of the six DNA segments were found to hybridize to various RNAs using the Northern technique and therefore contain portions of genes. One of the clones showed strong differential expression when epithelial tissues were compared to fibroblasts and may represent an epithelium-specific gene.",
"genre": "research_article",
"id": "sg:pub.10.1007/bf00193187",
"inLanguage": [
"en"
],
"isAccessibleForFree": false,
"isPartOf": [
{
"id": "sg:journal.1085982",
"issn": [
"0340-6717",
"1432-1203"
],
"name": "Human Genetics",
"type": "Periodical"
},
{
"issueNumber": "2",
"type": "PublicationIssue"
},
{
"type": "PublicationVolume",
"volumeNumber": "85"
}
],
"name": "Identification of sequences of chromosome 7 that are expressed in sweat gland epithelial cells",
"pagination": "151-156",
"productId": [
{
"name": "doi",
"type": "PropertyValue",
"value": [
"10.1007/bf00193187"
]
},
{
"name": "readcube_id",
"type": "PropertyValue",
"value": [
"955e91340c4b1a9e2285119cb2444d4f3855f335f3269bba678d490a29ddcd25"
]
},
{
"name": "dimensions_id",
"type": "PropertyValue",
"value": [
"pub.1031354867"
]
},
{
"name": "nlm_unique_id",
"type": "PropertyValue",
"value": [
"7613873"
]
},
{
"name": "pubmed_id",
"type": "PropertyValue",
"value": [
"1973401"
]
}
],
"sameAs": [
"https://doi.org/10.1007/bf00193187",
"https://app.dimensions.ai/details/publication/pub.1031354867"
],
"sdDataset": "articles",
"sdDatePublished": "2019-04-15T08:50",
"sdLicense": "https://scigraph.springernature.com/explorer/license/",
"sdPublisher": {
"name": "Springer Nature - SN SciGraph project",
"type": "Organization"
},
"sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000374_0000000374/records_119727_00000000.jsonl",
"type": "ScholarlyArticle",
"url": "http://link.springer.com/10.1007/BF00193187"
}
]
Download the RDF metadata as: json-ld nt turtle xml License info
JSON-LD is a popular format for linked data which is fully compatible with JSON.
curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/bf00193187'
N-Triples is a line-based linked data format ideal for batch operations.
curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/bf00193187'
Turtle is a human-readable linked data format.
curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/bf00193187'
RDF/XML is a standard XML format for linked data.
curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/bf00193187'
This table displays all metadata directly associated to this object as RDF triples.
257 TRIPLES
21 PREDICATES
76 URIs
34 LITERALS
22 BLANK NODES