Gene transfer in plants of Brassica juncea using Agrobacterium tumefaciens-mediated transformation View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1991-09

AUTHORS

Donna G. Barfield, Eng-Chong Pua

ABSTRACT

An efficient system for gene transfer into plants of Brassica juncea var. India Mustard, mediated by Agrobacterium tumefaciens. was developed through the manipulation of the culture medium and the use of the appropriate Agrobacterium strain. High frequency shoot regeneration (90-100%) was obtained from hypocotyl explants grown on medium containing 0.9% agarose, 3.3 mg/L AgNO3 and 0.5-2 mg/L BA in combination with 0.01-0.05 mg/L 2,4-D or 0.1-1 mg/L NAA. Of all the Agrobacterium strains tested, A. tumefaciens A208-SE, carrying the disarmed Ti plasmid and a binary vector pROA93, was the most effective for B. juncea transformation. pROA93 carries the coding sequences of the NPTII and the GUS genes, both driven by a common CaMV 35S promoter in two divergent directions. Inoculated explants grown on the selection medium in the presence of 0.5 mg/L BA and 0.1 mg/L NAA gave rise to transgenic shoots at the highest frequency (9%). All Ro transgenic plants were phenotypically normal, but variation in expression patterns of the GUS gene occurred among the transgenic plants in an organ- and tissue-specific manner. Both the NPTII and the GUS genes were transmitted to the R1 seed progeny and showed co-segregation. More... »

PAGES

308-314

References to SciGraph publications

  • 1987-12. Assaying chimeric genes in plants: The GUS gene fusion system in PLANT MOLECULAR BIOLOGY REPORTER
  • 1986-04. Leaf disc transformation of cultivated tomato (L. esculentum) using Agrobacterium tumefaciens in PLANT CELL REPORTS
  • 1988-03. Genetic transformation of cauliflower (Brassica oleracea L. var. Botrytis) by Agrobacterium rhizogenes in PLANT CELL REPORTS
  • 1989-08. Heavy metal tolerant transgenic Brassica napus L. and Nicotiana tabacum L. plants in THEORETICAL AND APPLIED GENETICS
  • 1985-12. Genetic manipulation in cultivars of oilseed rape (Brassica napus) using Agrobacterium in THEORETICAL AND APPLIED GENETICS
  • 1989-04. High efficiency transformation ofBrassica napus usingAgrobacterium vectors in PLANT CELL REPORTS
  • 1988-12. Transformation and regeneration of Brassica oleracea mediated by an oncogenic Agrobacterium tumefaciens in PLANT CELL REPORTS
  • 1989-06. High frequency transformation ofKalanchoe laciniata in PLANT CELL REPORTS
  • 1990-08. Effect of AgNO3 and aminoethoxyvinylglycine on in vitro shoot and root organogenesis from seedling explants of recalcitrant Brassica genotypes in PLANT CELL REPORTS
  • 1990-02. Engineering Resistance to Mixed Virus Infection in a Commercial Potato Cultivar: Resistance to Potato Virus X and Potato Virus Y in Transgenic Russet Burbank in BIO/TECHNOLOGY
  • 1989-05. Specificity of strain and genotype in the susceptibility of pea to Agrobacterium tumefaciens in PLANT CELL REPORTS
  • 1991-04. The Development of Virus-Resistant Alfalfa, Medicago sativa L in BIO/TECHNOLOGY
  • 1987-01. Expression of mouse dihydrofolate reductase gene confers methotrexate resistance in transgenic petunia plants in SOMATIC CELL AND MOLECULAR GENETICS
  • 1990-01. Localized transient expression of GUS in leaf discs following cocultivation with Agrobacterium in PLANT MOLECULAR BIOLOGY
  • 1985-02. Identification of DNA sequences required for activity of the cauliflower mosaic virus 35S promoter in NATURE
  • 1987-11. A novel mechanism of insect resistance engineered into tobacco in NATURE
  • 1986-07. Locations and stability of Agrobacterium-mediated T-DNA insertions in the Lycopersicon genome in MOLECULAR GENETICS AND GENOMICS
  • 1988-12. High efficiency Agrobacterium tumefaciens-mediated transformation of Arabidopsis thaliana leaf and cotyledon explants in PLANT CELL REPORTS
  • 1987-10. Transformation of Brassica napus with Agrobacterium tumefaciens based vectors in PLANT CELL REPORTS
  • 1989-05. Virulence of Agrobacterium tumefaciens strains with Brassica napus and Brassica juncea in PLANT CELL REPORTS
  • Journal

    TITLE

    Plant Cell Reports

    ISSUE

    6-7

    VOLUME

    10

    Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/bf00193148

    DOI

    http://dx.doi.org/10.1007/bf00193148

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1048934679

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/24221663


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