Primäre hepatozytenkulturen als modell zur experimentellen untersuchung der leberkonservierung View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1991-09

AUTHORS

R. Viebahn, H. de Groot, W. Lauchart, H. D. Becker

ABSTRACT

Primary hepatocyte cultures have been used to evaluate data concerning hypoxic liver cell injury. To show the suitability of this method in liver preservation studies hepatocyte cultures were incubated under different conditions: warm normoxia (37 degrees C, pO2 greater than 70 mm Hg), warm hypoxia (37 degrees C, pO2 less than 0.1 mm Hg), cold normoxia (4 degrees C, pO2 greater than 70 mm Hg) and cold hypoxia (4 degrees C, pO2 less than 0.1 mm Hg). Incubations were performed in Euro Collins solution (EC), University of Wisconsin solution of Belzer (UW) and histidine ketoglutarat tryptophan solution of Bretschneider (HTK) as well as in Krebs Henseleit buffer (KH) for control incubations. During 12 h of incubation hepatocyte cultures under warm normoxia lost viability continuously in EC, UW and HTK while in KH they remained stable. Under warm normoxia all cultures lost 50% of their viability during 12 h of incubation while in cold normoxia loss of viability was mild but significant. Under cold anoxia which is the standard condition of liver preservation the cultured hepatocytes remained unchanged for 12 h in KH, UW and HTK, while in EC most of the cells were dead after 6 h. It is concluded that incubations of primary hepatocyte cultures under different pO2 and temperatures are well suited to contribute to liver preservation studies on a preclinical level and thus may help to save animal experiments. More... »

PAGES

268-272

References to SciGraph publications

  • 1988-03. Ex situ Operation an der Leber Eine neue Möglichkeit in der Leberchirurgie in LANGENBECK'S ARCHIVES OF SURGERY
  • 1990-03. HTK-solution (Bretschneider) for human liver transplantation in LANGENBECK'S ARCHIVES OF SURGERY
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/bf00188266

    DOI

    http://dx.doi.org/10.1007/bf00188266

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1016042486

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/1791732


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