Control of gene expression from the SUC2 promoter of Saccharomyces cerevisiae with the aid of a glucose analyser View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1989-12

AUTHORS

Kong Hua Lin, Shinji Iijima, Kazuyuki Shimizu, Fumio Hishinuma, Takeshi Kobayashi

ABSTRACT

A Saccharomyces cerevisiae strain harbouring the recombinant plasmid pSMF38TMA was cultured in a jar fermentor under the control of glucose concentration. In the recombinant plasmid, the mouse α-amylase gene was fused to the S. cerevisiae SUC2 promoter. When glucose concentration in the medium was controlled at 10 g/l, the gene expression was completely repressed. On the other hand, the α-amylase was produced and secreted in the medium at a very high level, around 200 mg/l as evaluated from the specific activity of commercially available human salivary amylase, when the glucose was kept at 0.15 g/l. This amount was almost 20-fold that obtained at 10 g/l glucose. The specific growth rate of the yeast in this culture was almost 60% of that attained with 10 g/l glucose. To obtain higher cell growth and productivity, the yeast was at first cultured at 2 g/l glucose and the concentration was then lowered to 0.15 g/l. By this control of the glucose concentration, on-off regulation of gene expression from the SUC promoter could be attained. More... »

PAGES

313-316

References to SciGraph publications

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf00184981

DOI

http://dx.doi.org/10.1007/bf00184981

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1028209340


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