Ontology type: schema:ScholarlyArticle
1989-12
AUTHORSKong Hua Lin, Shinji Iijima, Kazuyuki Shimizu, Fumio Hishinuma, Takeshi Kobayashi
ABSTRACTA Saccharomyces cerevisiae strain harbouring the recombinant plasmid pSMF38TMA was cultured in a jar fermentor under the control of glucose concentration. In the recombinant plasmid, the mouse α-amylase gene was fused to the S. cerevisiae SUC2 promoter. When glucose concentration in the medium was controlled at 10 g/l, the gene expression was completely repressed. On the other hand, the α-amylase was produced and secreted in the medium at a very high level, around 200 mg/l as evaluated from the specific activity of commercially available human salivary amylase, when the glucose was kept at 0.15 g/l. This amount was almost 20-fold that obtained at 10 g/l glucose. The specific growth rate of the yeast in this culture was almost 60% of that attained with 10 g/l glucose. To obtain higher cell growth and productivity, the yeast was at first cultured at 2 g/l glucose and the concentration was then lowered to 0.15 g/l. By this control of the glucose concentration, on-off regulation of gene expression from the SUC promoter could be attained. More... »
PAGES313-316
http://scigraph.springernature.com/pub.10.1007/bf00184981
DOIhttp://dx.doi.org/10.1007/bf00184981
DIMENSIONShttps://app.dimensions.ai/details/publication/pub.1028209340
JSON-LD is the canonical representation for SciGraph data.
TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT
[
{
"@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json",
"about": [
{
"id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0604",
"inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/",
"name": "Genetics",
"type": "DefinedTerm"
},
{
"id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06",
"inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/",
"name": "Biological Sciences",
"type": "DefinedTerm"
}
],
"author": [
{
"affiliation": {
"alternateName": "Nagoya University",
"id": "https://www.grid.ac/institutes/grid.27476.30",
"name": [
"Department of Chemical Engineering, Nagoya University, Furo-cho, 464, Chikusa-ku, Nagoya, Japan"
],
"type": "Organization"
},
"familyName": "Hua Lin",
"givenName": "Kong",
"id": "sg:person.0703425210.38",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0703425210.38"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Nagoya University",
"id": "https://www.grid.ac/institutes/grid.27476.30",
"name": [
"Department of Chemical Engineering, Nagoya University, Furo-cho, 464, Chikusa-ku, Nagoya, Japan"
],
"type": "Organization"
},
"familyName": "Iijima",
"givenName": "Shinji",
"id": "sg:person.010627415051.52",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.010627415051.52"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Nagoya University",
"id": "https://www.grid.ac/institutes/grid.27476.30",
"name": [
"Department of Chemical Engineering, Nagoya University, Furo-cho, 464, Chikusa-ku, Nagoya, Japan"
],
"type": "Organization"
},
"familyName": "Shimizu",
"givenName": "Kazuyuki",
"id": "sg:person.0604417665.54",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0604417665.54"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Mitsubishi Chemical Holdings (Japan)",
"id": "https://www.grid.ac/institutes/grid.418306.8",
"name": [
"Mitsubishi-Kasei Institute of Life Science, Minamiooya, 194, Machida, Tokyo, Japan"
],
"type": "Organization"
},
"familyName": "Hishinuma",
"givenName": "Fumio",
"id": "sg:person.050633545.01",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.050633545.01"
],
"type": "Person"
},
{
"affiliation": {
"alternateName": "Nagoya University",
"id": "https://www.grid.ac/institutes/grid.27476.30",
"name": [
"Department of Chemical Engineering, Nagoya University, Furo-cho, 464, Chikusa-ku, Nagoya, Japan"
],
"type": "Organization"
},
"familyName": "Kobayashi",
"givenName": "Takeshi",
"id": "sg:person.012466626631.65",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.012466626631.65"
],
"type": "Person"
}
],
"citation": [
{
"id": "https://doi.org/10.1016/0385-6380(87)90095-1",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1002725487"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/0385-6380(87)90095-1",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1002725487"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1002/btpr.5420030108",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1005803744"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1002/btpr.5420030207",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1013501028"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1073/pnas.81.2.367",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1016806845"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1252/jcej.21.113",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1020636394"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1093/nar/16.15.7499",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1028368413"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1002/bit.260280209",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1030990961"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/0378-1119(84)90236-1",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1036863642"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/0378-1119(84)90236-1",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1036863642"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/0378-1119(83)90126-9",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1039950898"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/0378-1119(83)90126-9",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1039950898"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/0167-7799(87)90038-2",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1040544642"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1252/jcej.19.111",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1040968581"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/0385-6380(86)90074-9",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1043681102"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/0385-6380(86)90074-9",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1043681102"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/bf00253029",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1044727720",
"https://doi.org/10.1007/bf00253029"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/bf00253029",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1044727720",
"https://doi.org/10.1007/bf00253029"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/bf00253029",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1044727720",
"https://doi.org/10.1007/bf00253029"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1080/00021369.1987.10868041",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1048827925"
],
"type": "CreativeWork"
}
],
"datePublished": "1989-12",
"datePublishedReg": "1989-12-01",
"description": "A Saccharomyces cerevisiae strain harbouring the recombinant plasmid pSMF38TMA was cultured in a jar fermentor under the control of glucose concentration. In the recombinant plasmid, the mouse \u03b1-amylase gene was fused to the S. cerevisiae SUC2 promoter. When glucose concentration in the medium was controlled at 10 g/l, the gene expression was completely repressed. On the other hand, the \u03b1-amylase was produced and secreted in the medium at a very high level, around 200 mg/l as evaluated from the specific activity of commercially available human salivary amylase, when the glucose was kept at 0.15 g/l. This amount was almost 20-fold that obtained at 10 g/l glucose. The specific growth rate of the yeast in this culture was almost 60% of that attained with 10 g/l glucose. To obtain higher cell growth and productivity, the yeast was at first cultured at 2 g/l glucose and the concentration was then lowered to 0.15 g/l. By this control of the glucose concentration, on-off regulation of gene expression from the SUC promoter could be attained.",
"genre": "research_article",
"id": "sg:pub.10.1007/bf00184981",
"inLanguage": [
"en"
],
"isAccessibleForFree": false,
"isPartOf": [
{
"id": "sg:journal.1083533",
"issn": [
"0175-7598",
"1432-0614"
],
"name": "Applied Microbiology and Biotechnology",
"type": "Periodical"
},
{
"issueNumber": "3",
"type": "PublicationIssue"
},
{
"type": "PublicationVolume",
"volumeNumber": "32"
}
],
"name": "Control of gene expression from the SUC2 promoter of Saccharomyces cerevisiae with the aid of a glucose analyser",
"pagination": "313-316",
"productId": [
{
"name": "doi",
"type": "PropertyValue",
"value": [
"10.1007/bf00184981"
]
},
{
"name": "readcube_id",
"type": "PropertyValue",
"value": [
"86621f858c9f6dc2c601c33ae205f2dbcb409250a8fc8a942e8908070d4971fc"
]
},
{
"name": "dimensions_id",
"type": "PropertyValue",
"value": [
"pub.1028209340"
]
}
],
"sameAs": [
"https://doi.org/10.1007/bf00184981",
"https://app.dimensions.ai/details/publication/pub.1028209340"
],
"sdDataset": "articles",
"sdDatePublished": "2019-04-15T08:59",
"sdLicense": "https://scigraph.springernature.com/explorer/license/",
"sdPublisher": {
"name": "Springer Nature - SN SciGraph project",
"type": "Organization"
},
"sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000375_0000000375/records_91429_00000000.jsonl",
"type": "ScholarlyArticle",
"url": "http://link.springer.com/10.1007/BF00184981"
}
]Download the RDF metadata as: json-ld nt turtle xml License info
JSON-LD is a popular format for linked data which is fully compatible with JSON.
curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/bf00184981'
N-Triples is a line-based linked data format ideal for batch operations.
curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/bf00184981'
Turtle is a human-readable linked data format.
curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/bf00184981'
RDF/XML is a standard XML format for linked data.
curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/bf00184981'
This table displays all metadata directly associated to this object as RDF triples.
135 TRIPLES
21 PREDICATES
41 URIs
19 LITERALS
7 BLANK NODES