Evidence that stilbene synthases have developed from chalcone synthases several times in the course of evolution View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1994-06

AUTHORS

S. Tropf, T. Lanz, S.A. Rensing, J. Schröder, G. Schröder

ABSTRACT

Chalcone (CHS) and stilbene (STS) synthases are related plant-specific polyketide synthases that are key enzymes in the biosynthesis of flavonoids and of stilbene phytoalexins, respectively. A phylogenetic tree constructed from 34 CHS and four STS sequences revealed that the STS formed no separate cluster but grouped with CHS from the same or related plants. This suggested that STS evolved from CHS several times independently. We attempted to stimulate this by site-directed mutagenesis of an interfamily CHS/STS hybrid, which contained 107 amino acids of a CHS from Sinapis alba (N-terminal) and 287 amino acids of a STS from Arachis hypogaea. The hybrid had no enzyme activity. Three amino acid exchanges in the CHS part (Gln-100 to Glu, Val-103 to Met, Val-105 to Arg) were sufficient to obtain low STS activity, and one additional exchange (Gly-23 to Thr) resulted in 20-25% of the parent STS activity. A kinetic analysis indicated (1) that the hybrids had the same Km for the substrate 4-coumaroyl-CoA but a lower Vmax than the parent STS, and (2) that they had a different substrate preference than the parent STS and CHS. Most of the other mutations and their combinations led to enzymatically inactive protein aggregates, suggesting that the subunit folding and/or the dimerization was disturbed. We propose that STS evolved from CHS by a limited number of amino acid exchanges, and that the advantage gained by this enzyme function favored the selection of plants with improved STS activity. More... »

PAGES

610-618

References to SciGraph publications

  • 1987-12. Chalcone synthase genes in plants: A tool to study evolutionary relationships in JOURNAL OF MOLECULAR EVOLUTION
  • 1993. Phytoalexin Biosynthesis: Stilbene Synthase and Co-Action of a Reductase with Chalcone Synthase in MECHANISMS OF PLANT DEFENSE RESPONSES
  • 1988-11. Nucleotide sequences encoding two different chalcone synthases expressed in cotyledons of SAN 9789 treated mustard (Sinapis alba L.) in PLANT MOLECULAR BIOLOGY
  • 1986-03. Structure of the chalcone synthase gene of Antirrhinum majus in MOLECULAR GENETICS AND GENOMICS
  • 1993-05. Stress responses in alfalfa (Medicago sativa L.). 15. Characterization and expression patterns of members of a subset of the chalcone synthase multigene family in PLANT MOLECULAR BIOLOGY
  • 1990-05. Differential regulation of genes for resveratrol synthase in cell cultures ofArachis hypogaea L. in PLANTA
  • 1992-03. Molecular cloning of chalcone synthase cDNAs from Pisum sativum in PLANT MOLECULAR BIOLOGY
  • 1991-06. Structure of a chalcone synthase gene from Hordeum vulgare in PLANT MOLECULAR BIOLOGY
  • 1990-11. Molecular genetic analysis of chalcone synthase in Lycopersicon esculentum and an anthocyanin-deficient mutant in MOLECULAR GENETICS AND GENOMICS
  • 1985-05. Cloning and analysis of two genes for chalcone synthase from Petunia hybrida in MOLECULAR GENETICS AND GENOMICS
  • 1992-02. Molecular analysis of chalcone and dihydropinosylvin synthase from Scots pine (Pinus sylvestris), and differential regulation of these and related enzyme activities in stressed plants in PLANT MOLECULAR BIOLOGY
  • 1990-06. Isolation and sequence analysis of a chalcone synthase cDNA ofMatthiola incana R. Br. (Brassicaceae) in PLANT MOLECULAR BIOLOGY
  • 1987-12. Organization and differential activation of a gene family encoding the plant defense enzyme chalcone synthase in Phaseolus vulgaris in MOLECULAR GENETICS AND GENOMICS
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/bf00175881

    DOI

    http://dx.doi.org/10.1007/bf00175881

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1035604311

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/8083886


    Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
    Incoming Citations Browse incoming citations for this publication using opencitations.net

    JSON-LD is the canonical representation for SciGraph data.

    TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

    [
      {
        "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
        "about": [
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Biochemistry and Cell Biology", 
            "type": "DefinedTerm"
          }, 
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Biological Sciences", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Acyltransferases", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Amino Acid Sequence", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Base Sequence", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Biological Evolution", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Genes", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Molecular Sequence Data", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Phylogeny", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Plant Proteins", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Plants", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Sequence Alignment", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Sequence Homology", 
            "type": "DefinedTerm"
          }
        ], 
        "author": [
          {
            "affiliation": {
              "name": [
                "Institut f\u00fcr Biologie II, Universit\u00e4t Freiburg, Sch\u00e4nzlestr. 1, D-79104, Freiburg, Federal Republic of Germany"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Tropf", 
            "givenName": "S.", 
            "id": "sg:person.01206563746.76", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01206563746.76"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "name": [
                "Institut f\u00fcr Biologie II, Universit\u00e4t Freiburg, Sch\u00e4nzlestr. 1, D-79104, Freiburg, Federal Republic of Germany"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Lanz", 
            "givenName": "T.", 
            "id": "sg:person.01342276726.31", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01342276726.31"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "name": [
                "Institut f\u00fcr Biologie II, Universit\u00e4t Freiburg, Sch\u00e4nzlestr. 1, D-79104, Freiburg, Federal Republic of Germany"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Rensing", 
            "givenName": "S.A.", 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "name": [
                "Institut f\u00fcr Biologie II, Universit\u00e4t Freiburg, Sch\u00e4nzlestr. 1, D-79104, Freiburg, Federal Republic of Germany"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Schr\u00f6der", 
            "givenName": "J.", 
            "id": "sg:person.01321574576.06", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01321574576.06"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "name": [
                "Institut f\u00fcr Biologie II, Universit\u00e4t Freiburg, Sch\u00e4nzlestr. 1, D-79104, Freiburg, Federal Republic of Germany"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Schr\u00f6der", 
            "givenName": "G.", 
            "id": "sg:person.01001234426.41", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01001234426.41"
            ], 
            "type": "Person"
          }
        ], 
        "citation": [
          {
            "id": "sg:pub.10.1007/bf00271562", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1001650691", 
              "https://doi.org/10.1007/bf00271562"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00271562", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1001650691", 
              "https://doi.org/10.1007/bf00271562"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00040665", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1001906113", 
              "https://doi.org/10.1007/bf00040665"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00040665", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1001906113", 
              "https://doi.org/10.1007/bf00040665"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00019221", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1003195249", 
              "https://doi.org/10.1007/bf00019221"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00019221", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1003195249", 
              "https://doi.org/10.1007/bf00019221"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0968-0004(89)90131-x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1004361245"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0968-0004(89)90131-x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1004361245"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1214/aos/1176350142", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1010020927"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0378-1119(89)90399-5", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1011098702"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0378-1119(89)90399-5", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1011098702"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/b978-0-12-347880-1.50018-0", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1011203958"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0014-5793(92)80894-m", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1019680696"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1111/j.1432-1033.1988.tb13868.x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1020044009"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/b978-0-12-461011-8.50011-1", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1026083014"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1073/pnas.82.2.488", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1026991187"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0014-5793(92)81187-q", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1030409385"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf02411534", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1031746021", 
              "https://doi.org/10.1007/bf02411534"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf02411534", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1031746021", 
              "https://doi.org/10.1007/bf02411534"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0968-0004(89)90147-3", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1032955019"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0968-0004(89)90147-3", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1032955019"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00019405", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1034713948", 
              "https://doi.org/10.1007/bf00019405"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00019405", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1034713948", 
              "https://doi.org/10.1007/bf00019405"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1093/nar/18.19.5899", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1035531173"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00016087", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1036971149", 
              "https://doi.org/10.1007/bf00016087"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00016087", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1036971149", 
              "https://doi.org/10.1007/bf00016087"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00019526", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1037307821", 
              "https://doi.org/10.1007/bf00019526"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00019526", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1037307821", 
              "https://doi.org/10.1007/bf00019526"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1128/mcb.8.5.1985", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1040337381"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00333273", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1041964473", 
              "https://doi.org/10.1007/bf00333273"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00333273", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1041964473", 
              "https://doi.org/10.1007/bf00333273"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/978-94-011-1737-1_67", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1044720504", 
              "https://doi.org/10.1007/978-94-011-1737-1_67"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0378-1119(89)90185-6", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1046976186"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0378-1119(89)90185-6", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1046976186"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00325687", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1047686818", 
              "https://doi.org/10.1007/bf00325687"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00325687", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1047686818", 
              "https://doi.org/10.1007/bf00325687"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0014-5793(90)80961-h", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1048136323"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1248/cpb.39.1911", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1048395562"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00330261", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1049707976", 
              "https://doi.org/10.1007/bf00330261"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00330261", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1049707976", 
              "https://doi.org/10.1007/bf00330261"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00014932", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1050693848", 
              "https://doi.org/10.1007/bf00014932"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf00014932", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1050693848", 
              "https://doi.org/10.1007/bf00014932"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf02099854", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1051127231", 
              "https://doi.org/10.1007/bf02099854"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/bf02099854", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1051127231", 
              "https://doi.org/10.1007/bf02099854"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1042/bj2400001", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1053673110"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1042/bj2400001", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1053673110"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1093/bioinformatics/8.2.189", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1059414135"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://app.dimensions.ai/details/publication/pub.1076183309", 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://app.dimensions.ai/details/publication/pub.1076960216", 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1002/j.1460-2075.1983.tb01661.x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1077183614"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1002/j.1460-2075.1991.tb07802.x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1077331895"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://app.dimensions.ai/details/publication/pub.1077738732", 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://app.dimensions.ai/details/publication/pub.1078131555", 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1515/znc-1990-1-202", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1078437369"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://app.dimensions.ai/details/publication/pub.1079052218", 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://app.dimensions.ai/details/publication/pub.1082799099", 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1111/j.1558-5646.1985.tb00420.x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1085717760"
            ], 
            "type": "CreativeWork"
          }
        ], 
        "datePublished": "1994-06", 
        "datePublishedReg": "1994-06-01", 
        "description": "Chalcone (CHS) and stilbene (STS) synthases are related plant-specific polyketide synthases that are key enzymes in the biosynthesis of flavonoids and of stilbene phytoalexins, respectively. A phylogenetic tree constructed from 34 CHS and four STS sequences revealed that the STS formed no separate cluster but grouped with CHS from the same or related plants. This suggested that STS evolved from CHS several times independently. We attempted to stimulate this by site-directed mutagenesis of an interfamily CHS/STS hybrid, which contained 107 amino acids of a CHS from Sinapis alba (N-terminal) and 287 amino acids of a STS from Arachis hypogaea. The hybrid had no enzyme activity. Three amino acid exchanges in the CHS part (Gln-100 to Glu, Val-103 to Met, Val-105 to Arg) were sufficient to obtain low STS activity, and one additional exchange (Gly-23 to Thr) resulted in 20-25% of the parent STS activity. A kinetic analysis indicated (1) that the hybrids had the same Km for the substrate 4-coumaroyl-CoA but a lower Vmax than the parent STS, and (2) that they had a different substrate preference than the parent STS and CHS. Most of the other mutations and their combinations led to enzymatically inactive protein aggregates, suggesting that the subunit folding and/or the dimerization was disturbed. We propose that STS evolved from CHS by a limited number of amino acid exchanges, and that the advantage gained by this enzyme function favored the selection of plants with improved STS activity.", 
        "genre": "research_article", 
        "id": "sg:pub.10.1007/bf00175881", 
        "inLanguage": [
          "en"
        ], 
        "isAccessibleForFree": false, 
        "isPartOf": [
          {
            "id": "sg:journal.1016442", 
            "issn": [
              "0022-2844", 
              "1432-1432"
            ], 
            "name": "Journal of Molecular Evolution", 
            "type": "Periodical"
          }, 
          {
            "issueNumber": "6", 
            "type": "PublicationIssue"
          }, 
          {
            "type": "PublicationVolume", 
            "volumeNumber": "38"
          }
        ], 
        "name": "Evidence that stilbene synthases have developed from chalcone synthases several times in the course of evolution", 
        "pagination": "610-618", 
        "productId": [
          {
            "name": "doi", 
            "type": "PropertyValue", 
            "value": [
              "10.1007/bf00175881"
            ]
          }, 
          {
            "name": "readcube_id", 
            "type": "PropertyValue", 
            "value": [
              "a99b2dce40f90ea1e5c9d862e7a378dd943718bd529dbf35cafddba2b8a48de2"
            ]
          }, 
          {
            "name": "dimensions_id", 
            "type": "PropertyValue", 
            "value": [
              "pub.1035604311"
            ]
          }, 
          {
            "name": "nlm_unique_id", 
            "type": "PropertyValue", 
            "value": [
              "0360051"
            ]
          }, 
          {
            "name": "pubmed_id", 
            "type": "PropertyValue", 
            "value": [
              "8083886"
            ]
          }
        ], 
        "sameAs": [
          "https://doi.org/10.1007/bf00175881", 
          "https://app.dimensions.ai/details/publication/pub.1035604311"
        ], 
        "sdDataset": "articles", 
        "sdDatePublished": "2019-04-15T08:52", 
        "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
        "sdPublisher": {
          "name": "Springer Nature - SN SciGraph project", 
          "type": "Organization"
        }, 
        "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000374_0000000374/records_119746_00000000.jsonl", 
        "type": "ScholarlyArticle", 
        "url": "http://link.springer.com/10.1007/BF00175881"
      }
    ]
     

    Download the RDF metadata as:  json-ld nt turtle xml License info

    HOW TO GET THIS DATA PROGRAMMATICALLY:

    JSON-LD is a popular format for linked data which is fully compatible with JSON.

    curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/bf00175881'

    N-Triples is a line-based linked data format ideal for batch operations.

    curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/bf00175881'

    Turtle is a human-readable linked data format.

    curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/bf00175881'

    RDF/XML is a standard XML format for linked data.

    curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/bf00175881'


     

    This table displays all metadata directly associated to this object as RDF triples.

    274 TRIPLES      21 PREDICATES      80 URIs      32 LITERALS      20 BLANK NODES

    Subject Predicate Object
    1 sg:pub.10.1007/bf00175881 schema:about N05896cbde18744c5811a8a2c81de7373
    2 N085961d88ced4a65a365ef2273b6981d
    3 N1eef7e4dc16145018df26e743c5866e5
    4 N29ad3679b4d941a3af4e1a2a79ff4b75
    5 N2a8086fe0f6a4179a881c97d2d736266
    6 N6fcd6d1cb6b143f6ad691008db859c62
    7 N7ee6a3e21a5d4453878a3609bc6ef50c
    8 Nc58ec886676f4b48a76832d418baca85
    9 Ncaaa5591bd2642618269f5e1f644cf8d
    10 Ne4b5b333b54c433f99bdafb9064af086
    11 Nfed82ceba26f4a95ac800bf27f69841b
    12 anzsrc-for:06
    13 anzsrc-for:0601
    14 schema:author N2b1e987d257542d78b9e1cb53053e9f7
    15 schema:citation sg:pub.10.1007/978-94-011-1737-1_67
    16 sg:pub.10.1007/bf00014932
    17 sg:pub.10.1007/bf00016087
    18 sg:pub.10.1007/bf00019221
    19 sg:pub.10.1007/bf00019405
    20 sg:pub.10.1007/bf00019526
    21 sg:pub.10.1007/bf00040665
    22 sg:pub.10.1007/bf00271562
    23 sg:pub.10.1007/bf00325687
    24 sg:pub.10.1007/bf00330261
    25 sg:pub.10.1007/bf00333273
    26 sg:pub.10.1007/bf02099854
    27 sg:pub.10.1007/bf02411534
    28 https://app.dimensions.ai/details/publication/pub.1076183309
    29 https://app.dimensions.ai/details/publication/pub.1076960216
    30 https://app.dimensions.ai/details/publication/pub.1077738732
    31 https://app.dimensions.ai/details/publication/pub.1078131555
    32 https://app.dimensions.ai/details/publication/pub.1079052218
    33 https://app.dimensions.ai/details/publication/pub.1082799099
    34 https://doi.org/10.1002/j.1460-2075.1983.tb01661.x
    35 https://doi.org/10.1002/j.1460-2075.1991.tb07802.x
    36 https://doi.org/10.1016/0014-5793(90)80961-h
    37 https://doi.org/10.1016/0014-5793(92)80894-m
    38 https://doi.org/10.1016/0014-5793(92)81187-q
    39 https://doi.org/10.1016/0378-1119(89)90185-6
    40 https://doi.org/10.1016/0378-1119(89)90399-5
    41 https://doi.org/10.1016/0968-0004(89)90131-x
    42 https://doi.org/10.1016/0968-0004(89)90147-3
    43 https://doi.org/10.1016/b978-0-12-347880-1.50018-0
    44 https://doi.org/10.1016/b978-0-12-461011-8.50011-1
    45 https://doi.org/10.1042/bj2400001
    46 https://doi.org/10.1073/pnas.82.2.488
    47 https://doi.org/10.1093/bioinformatics/8.2.189
    48 https://doi.org/10.1093/nar/18.19.5899
    49 https://doi.org/10.1111/j.1432-1033.1988.tb13868.x
    50 https://doi.org/10.1111/j.1558-5646.1985.tb00420.x
    51 https://doi.org/10.1128/mcb.8.5.1985
    52 https://doi.org/10.1214/aos/1176350142
    53 https://doi.org/10.1248/cpb.39.1911
    54 https://doi.org/10.1515/znc-1990-1-202
    55 schema:datePublished 1994-06
    56 schema:datePublishedReg 1994-06-01
    57 schema:description Chalcone (CHS) and stilbene (STS) synthases are related plant-specific polyketide synthases that are key enzymes in the biosynthesis of flavonoids and of stilbene phytoalexins, respectively. A phylogenetic tree constructed from 34 CHS and four STS sequences revealed that the STS formed no separate cluster but grouped with CHS from the same or related plants. This suggested that STS evolved from CHS several times independently. We attempted to stimulate this by site-directed mutagenesis of an interfamily CHS/STS hybrid, which contained 107 amino acids of a CHS from Sinapis alba (N-terminal) and 287 amino acids of a STS from Arachis hypogaea. The hybrid had no enzyme activity. Three amino acid exchanges in the CHS part (Gln-100 to Glu, Val-103 to Met, Val-105 to Arg) were sufficient to obtain low STS activity, and one additional exchange (Gly-23 to Thr) resulted in 20-25% of the parent STS activity. A kinetic analysis indicated (1) that the hybrids had the same Km for the substrate 4-coumaroyl-CoA but a lower Vmax than the parent STS, and (2) that they had a different substrate preference than the parent STS and CHS. Most of the other mutations and their combinations led to enzymatically inactive protein aggregates, suggesting that the subunit folding and/or the dimerization was disturbed. We propose that STS evolved from CHS by a limited number of amino acid exchanges, and that the advantage gained by this enzyme function favored the selection of plants with improved STS activity.
    58 schema:genre research_article
    59 schema:inLanguage en
    60 schema:isAccessibleForFree false
    61 schema:isPartOf Nc7abcf69b57b4a6f93f3aa4557c776c6
    62 Nfcfb5b1d42e64c548fcadf2cbcd7a0d8
    63 sg:journal.1016442
    64 schema:name Evidence that stilbene synthases have developed from chalcone synthases several times in the course of evolution
    65 schema:pagination 610-618
    66 schema:productId N0ab58bfcb63d4809aa88f87639b9e17c
    67 N168ac9d9d87944609187b4a5b142fb05
    68 N3f547ef4b08e40e898e83beec0b66fec
    69 N6706eb92172d4dc7832e55363f441c0b
    70 Na8e62a89adcb407fb1b578f588d0d73f
    71 schema:sameAs https://app.dimensions.ai/details/publication/pub.1035604311
    72 https://doi.org/10.1007/bf00175881
    73 schema:sdDatePublished 2019-04-15T08:52
    74 schema:sdLicense https://scigraph.springernature.com/explorer/license/
    75 schema:sdPublisher N54a91c40318c44a0b8a104ff04558d4c
    76 schema:url http://link.springer.com/10.1007/BF00175881
    77 sgo:license sg:explorer/license/
    78 sgo:sdDataset articles
    79 rdf:type schema:ScholarlyArticle
    80 N05896cbde18744c5811a8a2c81de7373 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    81 schema:name Plants
    82 rdf:type schema:DefinedTerm
    83 N085961d88ced4a65a365ef2273b6981d schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    84 schema:name Plant Proteins
    85 rdf:type schema:DefinedTerm
    86 N0ab58bfcb63d4809aa88f87639b9e17c schema:name nlm_unique_id
    87 schema:value 0360051
    88 rdf:type schema:PropertyValue
    89 N168ac9d9d87944609187b4a5b142fb05 schema:name dimensions_id
    90 schema:value pub.1035604311
    91 rdf:type schema:PropertyValue
    92 N1eef7e4dc16145018df26e743c5866e5 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    93 schema:name Acyltransferases
    94 rdf:type schema:DefinedTerm
    95 N25ad8ccc841e420489aa5a30e9dc4efa schema:name Institut für Biologie II, Universität Freiburg, Schänzlestr. 1, D-79104, Freiburg, Federal Republic of Germany
    96 rdf:type schema:Organization
    97 N28fca77c8ee6461eb60c702291af553c rdf:first sg:person.01342276726.31
    98 rdf:rest N3b4aff6dba14403cb8d651230b542f6a
    99 N29ad3679b4d941a3af4e1a2a79ff4b75 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    100 schema:name Genes
    101 rdf:type schema:DefinedTerm
    102 N2a8086fe0f6a4179a881c97d2d736266 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    103 schema:name Amino Acid Sequence
    104 rdf:type schema:DefinedTerm
    105 N2b1e987d257542d78b9e1cb53053e9f7 rdf:first sg:person.01206563746.76
    106 rdf:rest N28fca77c8ee6461eb60c702291af553c
    107 N2e0b99fb13564b60a82f34c8810f7f4b schema:name Institut für Biologie II, Universität Freiburg, Schänzlestr. 1, D-79104, Freiburg, Federal Republic of Germany
    108 rdf:type schema:Organization
    109 N31435dc1611e4da192a6039b0aa148fa rdf:first sg:person.01321574576.06
    110 rdf:rest N72a06bc5195f4bd7ac0303cdb1fab1e1
    111 N3b4aff6dba14403cb8d651230b542f6a rdf:first N47c5179934ee4103bb85b5546ac8c51c
    112 rdf:rest N31435dc1611e4da192a6039b0aa148fa
    113 N3f547ef4b08e40e898e83beec0b66fec schema:name readcube_id
    114 schema:value a99b2dce40f90ea1e5c9d862e7a378dd943718bd529dbf35cafddba2b8a48de2
    115 rdf:type schema:PropertyValue
    116 N47c5179934ee4103bb85b5546ac8c51c schema:affiliation Nb81d7cb95cdd4cf7a20e579362d49b32
    117 schema:familyName Rensing
    118 schema:givenName S.A.
    119 rdf:type schema:Person
    120 N54a91c40318c44a0b8a104ff04558d4c schema:name Springer Nature - SN SciGraph project
    121 rdf:type schema:Organization
    122 N6706eb92172d4dc7832e55363f441c0b schema:name pubmed_id
    123 schema:value 8083886
    124 rdf:type schema:PropertyValue
    125 N6fcd6d1cb6b143f6ad691008db859c62 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    126 schema:name Base Sequence
    127 rdf:type schema:DefinedTerm
    128 N72a06bc5195f4bd7ac0303cdb1fab1e1 rdf:first sg:person.01001234426.41
    129 rdf:rest rdf:nil
    130 N753553f425cb47518abfd87c43c1479c schema:name Institut für Biologie II, Universität Freiburg, Schänzlestr. 1, D-79104, Freiburg, Federal Republic of Germany
    131 rdf:type schema:Organization
    132 N7ee6a3e21a5d4453878a3609bc6ef50c schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    133 schema:name Biological Evolution
    134 rdf:type schema:DefinedTerm
    135 N83f8724fae0141c39a0f284660a2fcbf schema:name Institut für Biologie II, Universität Freiburg, Schänzlestr. 1, D-79104, Freiburg, Federal Republic of Germany
    136 rdf:type schema:Organization
    137 Na8e62a89adcb407fb1b578f588d0d73f schema:name doi
    138 schema:value 10.1007/bf00175881
    139 rdf:type schema:PropertyValue
    140 Nb81d7cb95cdd4cf7a20e579362d49b32 schema:name Institut für Biologie II, Universität Freiburg, Schänzlestr. 1, D-79104, Freiburg, Federal Republic of Germany
    141 rdf:type schema:Organization
    142 Nc58ec886676f4b48a76832d418baca85 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    143 schema:name Sequence Alignment
    144 rdf:type schema:DefinedTerm
    145 Nc7abcf69b57b4a6f93f3aa4557c776c6 schema:volumeNumber 38
    146 rdf:type schema:PublicationVolume
    147 Ncaaa5591bd2642618269f5e1f644cf8d schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    148 schema:name Molecular Sequence Data
    149 rdf:type schema:DefinedTerm
    150 Ne4b5b333b54c433f99bdafb9064af086 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    151 schema:name Phylogeny
    152 rdf:type schema:DefinedTerm
    153 Nfcfb5b1d42e64c548fcadf2cbcd7a0d8 schema:issueNumber 6
    154 rdf:type schema:PublicationIssue
    155 Nfed82ceba26f4a95ac800bf27f69841b schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    156 schema:name Sequence Homology
    157 rdf:type schema:DefinedTerm
    158 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
    159 schema:name Biological Sciences
    160 rdf:type schema:DefinedTerm
    161 anzsrc-for:0601 schema:inDefinedTermSet anzsrc-for:
    162 schema:name Biochemistry and Cell Biology
    163 rdf:type schema:DefinedTerm
    164 sg:journal.1016442 schema:issn 0022-2844
    165 1432-1432
    166 schema:name Journal of Molecular Evolution
    167 rdf:type schema:Periodical
    168 sg:person.01001234426.41 schema:affiliation N753553f425cb47518abfd87c43c1479c
    169 schema:familyName Schröder
    170 schema:givenName G.
    171 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01001234426.41
    172 rdf:type schema:Person
    173 sg:person.01206563746.76 schema:affiliation N2e0b99fb13564b60a82f34c8810f7f4b
    174 schema:familyName Tropf
    175 schema:givenName S.
    176 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01206563746.76
    177 rdf:type schema:Person
    178 sg:person.01321574576.06 schema:affiliation N25ad8ccc841e420489aa5a30e9dc4efa
    179 schema:familyName Schröder
    180 schema:givenName J.
    181 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01321574576.06
    182 rdf:type schema:Person
    183 sg:person.01342276726.31 schema:affiliation N83f8724fae0141c39a0f284660a2fcbf
    184 schema:familyName Lanz
    185 schema:givenName T.
    186 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01342276726.31
    187 rdf:type schema:Person
    188 sg:pub.10.1007/978-94-011-1737-1_67 schema:sameAs https://app.dimensions.ai/details/publication/pub.1044720504
    189 https://doi.org/10.1007/978-94-011-1737-1_67
    190 rdf:type schema:CreativeWork
    191 sg:pub.10.1007/bf00014932 schema:sameAs https://app.dimensions.ai/details/publication/pub.1050693848
    192 https://doi.org/10.1007/bf00014932
    193 rdf:type schema:CreativeWork
    194 sg:pub.10.1007/bf00016087 schema:sameAs https://app.dimensions.ai/details/publication/pub.1036971149
    195 https://doi.org/10.1007/bf00016087
    196 rdf:type schema:CreativeWork
    197 sg:pub.10.1007/bf00019221 schema:sameAs https://app.dimensions.ai/details/publication/pub.1003195249
    198 https://doi.org/10.1007/bf00019221
    199 rdf:type schema:CreativeWork
    200 sg:pub.10.1007/bf00019405 schema:sameAs https://app.dimensions.ai/details/publication/pub.1034713948
    201 https://doi.org/10.1007/bf00019405
    202 rdf:type schema:CreativeWork
    203 sg:pub.10.1007/bf00019526 schema:sameAs https://app.dimensions.ai/details/publication/pub.1037307821
    204 https://doi.org/10.1007/bf00019526
    205 rdf:type schema:CreativeWork
    206 sg:pub.10.1007/bf00040665 schema:sameAs https://app.dimensions.ai/details/publication/pub.1001906113
    207 https://doi.org/10.1007/bf00040665
    208 rdf:type schema:CreativeWork
    209 sg:pub.10.1007/bf00271562 schema:sameAs https://app.dimensions.ai/details/publication/pub.1001650691
    210 https://doi.org/10.1007/bf00271562
    211 rdf:type schema:CreativeWork
    212 sg:pub.10.1007/bf00325687 schema:sameAs https://app.dimensions.ai/details/publication/pub.1047686818
    213 https://doi.org/10.1007/bf00325687
    214 rdf:type schema:CreativeWork
    215 sg:pub.10.1007/bf00330261 schema:sameAs https://app.dimensions.ai/details/publication/pub.1049707976
    216 https://doi.org/10.1007/bf00330261
    217 rdf:type schema:CreativeWork
    218 sg:pub.10.1007/bf00333273 schema:sameAs https://app.dimensions.ai/details/publication/pub.1041964473
    219 https://doi.org/10.1007/bf00333273
    220 rdf:type schema:CreativeWork
    221 sg:pub.10.1007/bf02099854 schema:sameAs https://app.dimensions.ai/details/publication/pub.1051127231
    222 https://doi.org/10.1007/bf02099854
    223 rdf:type schema:CreativeWork
    224 sg:pub.10.1007/bf02411534 schema:sameAs https://app.dimensions.ai/details/publication/pub.1031746021
    225 https://doi.org/10.1007/bf02411534
    226 rdf:type schema:CreativeWork
    227 https://app.dimensions.ai/details/publication/pub.1076183309 schema:CreativeWork
    228 https://app.dimensions.ai/details/publication/pub.1076960216 schema:CreativeWork
    229 https://app.dimensions.ai/details/publication/pub.1077738732 schema:CreativeWork
    230 https://app.dimensions.ai/details/publication/pub.1078131555 schema:CreativeWork
    231 https://app.dimensions.ai/details/publication/pub.1079052218 schema:CreativeWork
    232 https://app.dimensions.ai/details/publication/pub.1082799099 schema:CreativeWork
    233 https://doi.org/10.1002/j.1460-2075.1983.tb01661.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1077183614
    234 rdf:type schema:CreativeWork
    235 https://doi.org/10.1002/j.1460-2075.1991.tb07802.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1077331895
    236 rdf:type schema:CreativeWork
    237 https://doi.org/10.1016/0014-5793(90)80961-h schema:sameAs https://app.dimensions.ai/details/publication/pub.1048136323
    238 rdf:type schema:CreativeWork
    239 https://doi.org/10.1016/0014-5793(92)80894-m schema:sameAs https://app.dimensions.ai/details/publication/pub.1019680696
    240 rdf:type schema:CreativeWork
    241 https://doi.org/10.1016/0014-5793(92)81187-q schema:sameAs https://app.dimensions.ai/details/publication/pub.1030409385
    242 rdf:type schema:CreativeWork
    243 https://doi.org/10.1016/0378-1119(89)90185-6 schema:sameAs https://app.dimensions.ai/details/publication/pub.1046976186
    244 rdf:type schema:CreativeWork
    245 https://doi.org/10.1016/0378-1119(89)90399-5 schema:sameAs https://app.dimensions.ai/details/publication/pub.1011098702
    246 rdf:type schema:CreativeWork
    247 https://doi.org/10.1016/0968-0004(89)90131-x schema:sameAs https://app.dimensions.ai/details/publication/pub.1004361245
    248 rdf:type schema:CreativeWork
    249 https://doi.org/10.1016/0968-0004(89)90147-3 schema:sameAs https://app.dimensions.ai/details/publication/pub.1032955019
    250 rdf:type schema:CreativeWork
    251 https://doi.org/10.1016/b978-0-12-347880-1.50018-0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1011203958
    252 rdf:type schema:CreativeWork
    253 https://doi.org/10.1016/b978-0-12-461011-8.50011-1 schema:sameAs https://app.dimensions.ai/details/publication/pub.1026083014
    254 rdf:type schema:CreativeWork
    255 https://doi.org/10.1042/bj2400001 schema:sameAs https://app.dimensions.ai/details/publication/pub.1053673110
    256 rdf:type schema:CreativeWork
    257 https://doi.org/10.1073/pnas.82.2.488 schema:sameAs https://app.dimensions.ai/details/publication/pub.1026991187
    258 rdf:type schema:CreativeWork
    259 https://doi.org/10.1093/bioinformatics/8.2.189 schema:sameAs https://app.dimensions.ai/details/publication/pub.1059414135
    260 rdf:type schema:CreativeWork
    261 https://doi.org/10.1093/nar/18.19.5899 schema:sameAs https://app.dimensions.ai/details/publication/pub.1035531173
    262 rdf:type schema:CreativeWork
    263 https://doi.org/10.1111/j.1432-1033.1988.tb13868.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1020044009
    264 rdf:type schema:CreativeWork
    265 https://doi.org/10.1111/j.1558-5646.1985.tb00420.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1085717760
    266 rdf:type schema:CreativeWork
    267 https://doi.org/10.1128/mcb.8.5.1985 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040337381
    268 rdf:type schema:CreativeWork
    269 https://doi.org/10.1214/aos/1176350142 schema:sameAs https://app.dimensions.ai/details/publication/pub.1010020927
    270 rdf:type schema:CreativeWork
    271 https://doi.org/10.1248/cpb.39.1911 schema:sameAs https://app.dimensions.ai/details/publication/pub.1048395562
    272 rdf:type schema:CreativeWork
    273 https://doi.org/10.1515/znc-1990-1-202 schema:sameAs https://app.dimensions.ai/details/publication/pub.1078437369
    274 rdf:type schema:CreativeWork
     




    Preview window. Press ESC to close (or click here)


    ...