Inhibition of pyrimidine de novo synthesis by DUP-785 (NSC 368390) View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1987-09

AUTHORS

G. J. Peters, S. L. Sharma, E. Laurensse, H. M. Pinedo

ABSTRACT

The mechanism of action of NSC 368390 (DUP-785, 6-fluoro-2-(2'-fluoro-1, 1'-biphenyl-4-yl)-3-methyl-4-quinoline carboxylic acid sodium salt) was studied using three different approaches. First, we studied growth inhibition by DUP-785 in L1210 leukemia cells and M5 melanoma cells. The concentrations causing 50% growth inhibition after 48 hr of culture were 5.8 and 0.6 microM, respectively. DUP-785 had to be present continuously throughout culture. Growth inhibition by 25 microM DUP-785 could be prevented by addition of 1 mM uridine or orotic acid to cultures of these cell lines; in M5 cells cytidine was also able to prevent growth inhibition. Dihydro-orotic acid (DHO) and carbamyl-aspartate were not able to prevent growth inhibition by DUP-785. Second, we studied accumulation of orotic acid and of orotidine induced by incubation with 1 microM pyrazofurin, an inhibitor of the orotate phosphoribosyl-transferase-orotidine-monophosphate decarboxylase complex. Addition of DUP-785 to the culture medium prevented the orotic acid accumulation. Furthermore, DUP-785 prevented accumulation of H14CO3- into orotic acid of pyrazofurin-treated L1210 cells. Third, we measured the effect of DUP-785 on DHO-dehydrogenase (DHO-DH), since the results indicated that this enzyme was affected by DUP-785. DHO-DH was assayed in isolated rat liver mitochondria. The Km for L-DHO was about 12 microM. DUP-785 appeared to be a potent inhibitor of DHO-DH with an apparent Ki of about 0.1 microM and an apparent Ki' of about 0.8 microM. The mode of inhibition appeared to be linear mixed type. After exposure of L1210 cells to 25 microM DUP-785 for 2 hr DHO-DH was almost completely inhibited. After suspension in fresh medium without drug, DHO-DH activity was recovered to about 60% after 24 hr. In conclusion, DUP-785 is a potent inhibitor of pyrimidine de novo biosynthesis, by inhibition of the mitochondrial enzyme DHO-DH. More... »

PAGES

235-244

References to SciGraph publications

  • 1984. Pyrimidine Metabolism in Rat Brain Cortex and Liver in PURINE METABOLISM IN MAN-IV
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/bf00175293

    DOI

    http://dx.doi.org/10.1007/bf00175293

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1017527894

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/2822596


    Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
    Incoming Citations Browse incoming citations for this publication using opencitations.net

    JSON-LD is the canonical representation for SciGraph data.

    TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

    [
      {
        "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
        "about": [
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Biochemistry and Cell Biology", 
            "type": "DefinedTerm"
          }, 
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Biological Sciences", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Animals", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Antineoplastic Agents", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Biphenyl Compounds", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Dihydroorotate Oxidase", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Leukemia L1210", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Orotic Acid", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Pyrimidines", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Ribonucleosides", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Tumor Cells, Cultured", 
            "type": "DefinedTerm"
          }
        ], 
        "author": [
          {
            "affiliation": {
              "alternateName": "VU University Medical Center", 
              "id": "https://www.grid.ac/institutes/grid.16872.3a", 
              "name": [
                "Department Oncology, Free University Hospital, P.O. Box 7057, 1007, Amsterdam, MB, The Netherlands"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Peters", 
            "givenName": "G. J.", 
            "id": "sg:person.01255301342.17", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01255301342.17"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "VU University Medical Center", 
              "id": "https://www.grid.ac/institutes/grid.16872.3a", 
              "name": [
                "Department Oncology, Free University Hospital, P.O. Box 7057, 1007, Amsterdam, MB, The Netherlands"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Sharma", 
            "givenName": "S. L.", 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "VU University Medical Center", 
              "id": "https://www.grid.ac/institutes/grid.16872.3a", 
              "name": [
                "Department Oncology, Free University Hospital, P.O. Box 7057, 1007, Amsterdam, MB, The Netherlands"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Laurensse", 
            "givenName": "E.", 
            "id": "sg:person.01217753643.01", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01217753643.01"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "VU University Medical Center", 
              "id": "https://www.grid.ac/institutes/grid.16872.3a", 
              "name": [
                "Department Oncology, Free University Hospital, P.O. Box 7057, 1007, Amsterdam, MB, The Netherlands"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Pinedo", 
            "givenName": "H. M.", 
            "id": "sg:person.013437631522.70", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.013437631522.70"
            ], 
            "type": "Person"
          }
        ], 
        "citation": [
          {
            "id": "https://doi.org/10.1042/bj1370143", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1001381114"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1042/bj1370143", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1001381114"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/s0378-4347(00)84123-7", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1002629897"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1111/j.1432-1033.1980.tb04641.x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1005341263"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0006-2952(76)90425-1", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1005636141"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0006-2952(76)90425-1", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1005636141"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0003-9861(76)90252-6", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1006013079"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0005-2787(68)90210-4", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1019415958"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0005-2787(68)90210-4", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1019415958"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0003-2697(87)90647-6", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1036342840"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1007/978-1-4684-4553-4_102", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1038778539", 
              "https://doi.org/10.1007/978-1-4684-4553-4_102"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0003-9861(76)90143-0", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1039683190"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1159/000241162", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1039820300"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0006-2952(77)90137-x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1051614060"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0006-2952(77)90137-x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1051614060"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1146/annurev.bi.49.070180.001345", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1052711917"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://app.dimensions.ai/details/publication/pub.1078587618", 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://app.dimensions.ai/details/publication/pub.1078803298", 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://app.dimensions.ai/details/publication/pub.1079275653", 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://app.dimensions.ai/details/publication/pub.1079509999", 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://app.dimensions.ai/details/publication/pub.1079922945", 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://app.dimensions.ai/details/publication/pub.1080102667", 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://app.dimensions.ai/details/publication/pub.1080435723", 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://app.dimensions.ai/details/publication/pub.1081670648", 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://app.dimensions.ai/details/publication/pub.1081853510", 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://app.dimensions.ai/details/publication/pub.1081960560", 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://app.dimensions.ai/details/publication/pub.1082367180", 
            "type": "CreativeWork"
          }
        ], 
        "datePublished": "1987-09", 
        "datePublishedReg": "1987-09-01", 
        "description": "The mechanism of action of NSC 368390 (DUP-785, 6-fluoro-2-(2'-fluoro-1, 1'-biphenyl-4-yl)-3-methyl-4-quinoline carboxylic acid sodium salt) was studied using three different approaches. First, we studied growth inhibition by DUP-785 in L1210 leukemia cells and M5 melanoma cells. The concentrations causing 50% growth inhibition after 48 hr of culture were 5.8 and 0.6 microM, respectively. DUP-785 had to be present continuously throughout culture. Growth inhibition by 25 microM DUP-785 could be prevented by addition of 1 mM uridine or orotic acid to cultures of these cell lines; in M5 cells cytidine was also able to prevent growth inhibition. Dihydro-orotic acid (DHO) and carbamyl-aspartate were not able to prevent growth inhibition by DUP-785. Second, we studied accumulation of orotic acid and of orotidine induced by incubation with 1 microM pyrazofurin, an inhibitor of the orotate phosphoribosyl-transferase-orotidine-monophosphate decarboxylase complex. Addition of DUP-785 to the culture medium prevented the orotic acid accumulation. Furthermore, DUP-785 prevented accumulation of H14CO3- into orotic acid of pyrazofurin-treated L1210 cells. Third, we measured the effect of DUP-785 on DHO-dehydrogenase (DHO-DH), since the results indicated that this enzyme was affected by DUP-785. DHO-DH was assayed in isolated rat liver mitochondria. The Km for L-DHO was about 12 microM. DUP-785 appeared to be a potent inhibitor of DHO-DH with an apparent Ki of about 0.1 microM and an apparent Ki' of about 0.8 microM. The mode of inhibition appeared to be linear mixed type. After exposure of L1210 cells to 25 microM DUP-785 for 2 hr DHO-DH was almost completely inhibited. After suspension in fresh medium without drug, DHO-DH activity was recovered to about 60% after 24 hr. In conclusion, DUP-785 is a potent inhibitor of pyrimidine de novo biosynthesis, by inhibition of the mitochondrial enzyme DHO-DH.", 
        "genre": "research_article", 
        "id": "sg:pub.10.1007/bf00175293", 
        "inLanguage": [
          "en"
        ], 
        "isAccessibleForFree": false, 
        "isPartOf": [
          {
            "id": "sg:journal.1094201", 
            "issn": [
              "0167-6997", 
              "1573-0646"
            ], 
            "name": "Investigational New Drugs", 
            "type": "Periodical"
          }, 
          {
            "issueNumber": "3", 
            "type": "PublicationIssue"
          }, 
          {
            "type": "PublicationVolume", 
            "volumeNumber": "5"
          }
        ], 
        "name": "Inhibition of pyrimidine de novo synthesis by DUP-785 (NSC 368390)", 
        "pagination": "235-244", 
        "productId": [
          {
            "name": "doi", 
            "type": "PropertyValue", 
            "value": [
              "10.1007/bf00175293"
            ]
          }, 
          {
            "name": "readcube_id", 
            "type": "PropertyValue", 
            "value": [
              "5382820e80ba180b6b387ffc1a3c8da518b272e4d83a7aab62b100996603722e"
            ]
          }, 
          {
            "name": "dimensions_id", 
            "type": "PropertyValue", 
            "value": [
              "pub.1017527894"
            ]
          }, 
          {
            "name": "nlm_unique_id", 
            "type": "PropertyValue", 
            "value": [
              "8309330"
            ]
          }, 
          {
            "name": "pubmed_id", 
            "type": "PropertyValue", 
            "value": [
              "2822596"
            ]
          }
        ], 
        "sameAs": [
          "https://doi.org/10.1007/bf00175293", 
          "https://app.dimensions.ai/details/publication/pub.1017527894"
        ], 
        "sdDataset": "articles", 
        "sdDatePublished": "2019-04-15T08:54", 
        "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
        "sdPublisher": {
          "name": "Springer Nature - SN SciGraph project", 
          "type": "Organization"
        }, 
        "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000374_0000000374/records_119752_00000000.jsonl", 
        "type": "ScholarlyArticle", 
        "url": "http://link.springer.com/10.1007/BF00175293"
      }
    ]
     

    Download the RDF metadata as:  json-ld nt turtle xml License info

    HOW TO GET THIS DATA PROGRAMMATICALLY:

    JSON-LD is a popular format for linked data which is fully compatible with JSON.

    curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/bf00175293'

    N-Triples is a line-based linked data format ideal for batch operations.

    curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/bf00175293'

    Turtle is a human-readable linked data format.

    curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/bf00175293'

    RDF/XML is a standard XML format for linked data.

    curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/bf00175293'


     

    This table displays all metadata directly associated to this object as RDF triples.

    184 TRIPLES      21 PREDICATES      61 URIs      30 LITERALS      18 BLANK NODES

    Subject Predicate Object
    1 sg:pub.10.1007/bf00175293 schema:about N1f193573ceed44ab89445031ee34ec52
    2 N2e0d23e4ceff4a5c94ec127476f99fae
    3 N6767e2b3b9424596bf85ccdac83a4d3e
    4 N8962c4a9f9684315a809437d104c0f08
    5 Nbf1319f5055b4ec5b7314daa2f9b078e
    6 Nc406e60aac8e4bc1a7bccc7c8acf0103
    7 Ncb31989c41104710928dffa067a10fb7
    8 Nda0e3256724a4cc9b50711fd14858640
    9 Nfeb0c8ee916c4de6942dc9e62392d02f
    10 anzsrc-for:06
    11 anzsrc-for:0601
    12 schema:author N0f0085c570bd4742988695eacd4e8b1e
    13 schema:citation sg:pub.10.1007/978-1-4684-4553-4_102
    14 https://app.dimensions.ai/details/publication/pub.1078587618
    15 https://app.dimensions.ai/details/publication/pub.1078803298
    16 https://app.dimensions.ai/details/publication/pub.1079275653
    17 https://app.dimensions.ai/details/publication/pub.1079509999
    18 https://app.dimensions.ai/details/publication/pub.1079922945
    19 https://app.dimensions.ai/details/publication/pub.1080102667
    20 https://app.dimensions.ai/details/publication/pub.1080435723
    21 https://app.dimensions.ai/details/publication/pub.1081670648
    22 https://app.dimensions.ai/details/publication/pub.1081853510
    23 https://app.dimensions.ai/details/publication/pub.1081960560
    24 https://app.dimensions.ai/details/publication/pub.1082367180
    25 https://doi.org/10.1016/0003-2697(87)90647-6
    26 https://doi.org/10.1016/0003-9861(76)90143-0
    27 https://doi.org/10.1016/0003-9861(76)90252-6
    28 https://doi.org/10.1016/0005-2787(68)90210-4
    29 https://doi.org/10.1016/0006-2952(76)90425-1
    30 https://doi.org/10.1016/0006-2952(77)90137-x
    31 https://doi.org/10.1016/s0378-4347(00)84123-7
    32 https://doi.org/10.1042/bj1370143
    33 https://doi.org/10.1111/j.1432-1033.1980.tb04641.x
    34 https://doi.org/10.1146/annurev.bi.49.070180.001345
    35 https://doi.org/10.1159/000241162
    36 schema:datePublished 1987-09
    37 schema:datePublishedReg 1987-09-01
    38 schema:description The mechanism of action of NSC 368390 (DUP-785, 6-fluoro-2-(2'-fluoro-1, 1'-biphenyl-4-yl)-3-methyl-4-quinoline carboxylic acid sodium salt) was studied using three different approaches. First, we studied growth inhibition by DUP-785 in L1210 leukemia cells and M5 melanoma cells. The concentrations causing 50% growth inhibition after 48 hr of culture were 5.8 and 0.6 microM, respectively. DUP-785 had to be present continuously throughout culture. Growth inhibition by 25 microM DUP-785 could be prevented by addition of 1 mM uridine or orotic acid to cultures of these cell lines; in M5 cells cytidine was also able to prevent growth inhibition. Dihydro-orotic acid (DHO) and carbamyl-aspartate were not able to prevent growth inhibition by DUP-785. Second, we studied accumulation of orotic acid and of orotidine induced by incubation with 1 microM pyrazofurin, an inhibitor of the orotate phosphoribosyl-transferase-orotidine-monophosphate decarboxylase complex. Addition of DUP-785 to the culture medium prevented the orotic acid accumulation. Furthermore, DUP-785 prevented accumulation of H14CO3- into orotic acid of pyrazofurin-treated L1210 cells. Third, we measured the effect of DUP-785 on DHO-dehydrogenase (DHO-DH), since the results indicated that this enzyme was affected by DUP-785. DHO-DH was assayed in isolated rat liver mitochondria. The Km for L-DHO was about 12 microM. DUP-785 appeared to be a potent inhibitor of DHO-DH with an apparent Ki of about 0.1 microM and an apparent Ki' of about 0.8 microM. The mode of inhibition appeared to be linear mixed type. After exposure of L1210 cells to 25 microM DUP-785 for 2 hr DHO-DH was almost completely inhibited. After suspension in fresh medium without drug, DHO-DH activity was recovered to about 60% after 24 hr. In conclusion, DUP-785 is a potent inhibitor of pyrimidine de novo biosynthesis, by inhibition of the mitochondrial enzyme DHO-DH.
    39 schema:genre research_article
    40 schema:inLanguage en
    41 schema:isAccessibleForFree false
    42 schema:isPartOf N7557bd3230004287b8ffbf04b981e67b
    43 N7dcf0a87dc214967bc1a872d42788ed7
    44 sg:journal.1094201
    45 schema:name Inhibition of pyrimidine de novo synthesis by DUP-785 (NSC 368390)
    46 schema:pagination 235-244
    47 schema:productId N575e254c04ee4344b17f261c9f4dea24
    48 Nc979d1a7936541b4843f1c89ff3104e0
    49 Ncd44f8e86b734e869cc6e4f260651937
    50 Ne3fe80ccf1a449b78abdace341e193f2
    51 Nf65176ea5dd044b9a9c68cc1891e58a9
    52 schema:sameAs https://app.dimensions.ai/details/publication/pub.1017527894
    53 https://doi.org/10.1007/bf00175293
    54 schema:sdDatePublished 2019-04-15T08:54
    55 schema:sdLicense https://scigraph.springernature.com/explorer/license/
    56 schema:sdPublisher Nb0cecbb173ff44499c631ed3f12b46d7
    57 schema:url http://link.springer.com/10.1007/BF00175293
    58 sgo:license sg:explorer/license/
    59 sgo:sdDataset articles
    60 rdf:type schema:ScholarlyArticle
    61 N0f0085c570bd4742988695eacd4e8b1e rdf:first sg:person.01255301342.17
    62 rdf:rest N68daa8d78a8c4c4e869c1c28e27ef92c
    63 N1f193573ceed44ab89445031ee34ec52 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    64 schema:name Orotic Acid
    65 rdf:type schema:DefinedTerm
    66 N2e0d23e4ceff4a5c94ec127476f99fae schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    67 schema:name Tumor Cells, Cultured
    68 rdf:type schema:DefinedTerm
    69 N36bb667352ac4e0e80b5cb419f9bf6c0 rdf:first sg:person.013437631522.70
    70 rdf:rest rdf:nil
    71 N575e254c04ee4344b17f261c9f4dea24 schema:name doi
    72 schema:value 10.1007/bf00175293
    73 rdf:type schema:PropertyValue
    74 N5f2cc479be6e4414b5204c6700831fea rdf:first sg:person.01217753643.01
    75 rdf:rest N36bb667352ac4e0e80b5cb419f9bf6c0
    76 N6767e2b3b9424596bf85ccdac83a4d3e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    77 schema:name Antineoplastic Agents
    78 rdf:type schema:DefinedTerm
    79 N68daa8d78a8c4c4e869c1c28e27ef92c rdf:first N7a855ab371d646d1a59ad26a6c3f837d
    80 rdf:rest N5f2cc479be6e4414b5204c6700831fea
    81 N7557bd3230004287b8ffbf04b981e67b schema:volumeNumber 5
    82 rdf:type schema:PublicationVolume
    83 N7a855ab371d646d1a59ad26a6c3f837d schema:affiliation https://www.grid.ac/institutes/grid.16872.3a
    84 schema:familyName Sharma
    85 schema:givenName S. L.
    86 rdf:type schema:Person
    87 N7dcf0a87dc214967bc1a872d42788ed7 schema:issueNumber 3
    88 rdf:type schema:PublicationIssue
    89 N8962c4a9f9684315a809437d104c0f08 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    90 schema:name Ribonucleosides
    91 rdf:type schema:DefinedTerm
    92 Nb0cecbb173ff44499c631ed3f12b46d7 schema:name Springer Nature - SN SciGraph project
    93 rdf:type schema:Organization
    94 Nbf1319f5055b4ec5b7314daa2f9b078e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    95 schema:name Leukemia L1210
    96 rdf:type schema:DefinedTerm
    97 Nc406e60aac8e4bc1a7bccc7c8acf0103 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    98 schema:name Pyrimidines
    99 rdf:type schema:DefinedTerm
    100 Nc979d1a7936541b4843f1c89ff3104e0 schema:name dimensions_id
    101 schema:value pub.1017527894
    102 rdf:type schema:PropertyValue
    103 Ncb31989c41104710928dffa067a10fb7 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    104 schema:name Dihydroorotate Oxidase
    105 rdf:type schema:DefinedTerm
    106 Ncd44f8e86b734e869cc6e4f260651937 schema:name pubmed_id
    107 schema:value 2822596
    108 rdf:type schema:PropertyValue
    109 Nda0e3256724a4cc9b50711fd14858640 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    110 schema:name Biphenyl Compounds
    111 rdf:type schema:DefinedTerm
    112 Ne3fe80ccf1a449b78abdace341e193f2 schema:name nlm_unique_id
    113 schema:value 8309330
    114 rdf:type schema:PropertyValue
    115 Nf65176ea5dd044b9a9c68cc1891e58a9 schema:name readcube_id
    116 schema:value 5382820e80ba180b6b387ffc1a3c8da518b272e4d83a7aab62b100996603722e
    117 rdf:type schema:PropertyValue
    118 Nfeb0c8ee916c4de6942dc9e62392d02f schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    119 schema:name Animals
    120 rdf:type schema:DefinedTerm
    121 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
    122 schema:name Biological Sciences
    123 rdf:type schema:DefinedTerm
    124 anzsrc-for:0601 schema:inDefinedTermSet anzsrc-for:
    125 schema:name Biochemistry and Cell Biology
    126 rdf:type schema:DefinedTerm
    127 sg:journal.1094201 schema:issn 0167-6997
    128 1573-0646
    129 schema:name Investigational New Drugs
    130 rdf:type schema:Periodical
    131 sg:person.01217753643.01 schema:affiliation https://www.grid.ac/institutes/grid.16872.3a
    132 schema:familyName Laurensse
    133 schema:givenName E.
    134 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01217753643.01
    135 rdf:type schema:Person
    136 sg:person.01255301342.17 schema:affiliation https://www.grid.ac/institutes/grid.16872.3a
    137 schema:familyName Peters
    138 schema:givenName G. J.
    139 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01255301342.17
    140 rdf:type schema:Person
    141 sg:person.013437631522.70 schema:affiliation https://www.grid.ac/institutes/grid.16872.3a
    142 schema:familyName Pinedo
    143 schema:givenName H. M.
    144 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.013437631522.70
    145 rdf:type schema:Person
    146 sg:pub.10.1007/978-1-4684-4553-4_102 schema:sameAs https://app.dimensions.ai/details/publication/pub.1038778539
    147 https://doi.org/10.1007/978-1-4684-4553-4_102
    148 rdf:type schema:CreativeWork
    149 https://app.dimensions.ai/details/publication/pub.1078587618 schema:CreativeWork
    150 https://app.dimensions.ai/details/publication/pub.1078803298 schema:CreativeWork
    151 https://app.dimensions.ai/details/publication/pub.1079275653 schema:CreativeWork
    152 https://app.dimensions.ai/details/publication/pub.1079509999 schema:CreativeWork
    153 https://app.dimensions.ai/details/publication/pub.1079922945 schema:CreativeWork
    154 https://app.dimensions.ai/details/publication/pub.1080102667 schema:CreativeWork
    155 https://app.dimensions.ai/details/publication/pub.1080435723 schema:CreativeWork
    156 https://app.dimensions.ai/details/publication/pub.1081670648 schema:CreativeWork
    157 https://app.dimensions.ai/details/publication/pub.1081853510 schema:CreativeWork
    158 https://app.dimensions.ai/details/publication/pub.1081960560 schema:CreativeWork
    159 https://app.dimensions.ai/details/publication/pub.1082367180 schema:CreativeWork
    160 https://doi.org/10.1016/0003-2697(87)90647-6 schema:sameAs https://app.dimensions.ai/details/publication/pub.1036342840
    161 rdf:type schema:CreativeWork
    162 https://doi.org/10.1016/0003-9861(76)90143-0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1039683190
    163 rdf:type schema:CreativeWork
    164 https://doi.org/10.1016/0003-9861(76)90252-6 schema:sameAs https://app.dimensions.ai/details/publication/pub.1006013079
    165 rdf:type schema:CreativeWork
    166 https://doi.org/10.1016/0005-2787(68)90210-4 schema:sameAs https://app.dimensions.ai/details/publication/pub.1019415958
    167 rdf:type schema:CreativeWork
    168 https://doi.org/10.1016/0006-2952(76)90425-1 schema:sameAs https://app.dimensions.ai/details/publication/pub.1005636141
    169 rdf:type schema:CreativeWork
    170 https://doi.org/10.1016/0006-2952(77)90137-x schema:sameAs https://app.dimensions.ai/details/publication/pub.1051614060
    171 rdf:type schema:CreativeWork
    172 https://doi.org/10.1016/s0378-4347(00)84123-7 schema:sameAs https://app.dimensions.ai/details/publication/pub.1002629897
    173 rdf:type schema:CreativeWork
    174 https://doi.org/10.1042/bj1370143 schema:sameAs https://app.dimensions.ai/details/publication/pub.1001381114
    175 rdf:type schema:CreativeWork
    176 https://doi.org/10.1111/j.1432-1033.1980.tb04641.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1005341263
    177 rdf:type schema:CreativeWork
    178 https://doi.org/10.1146/annurev.bi.49.070180.001345 schema:sameAs https://app.dimensions.ai/details/publication/pub.1052711917
    179 rdf:type schema:CreativeWork
    180 https://doi.org/10.1159/000241162 schema:sameAs https://app.dimensions.ai/details/publication/pub.1039820300
    181 rdf:type schema:CreativeWork
    182 https://www.grid.ac/institutes/grid.16872.3a schema:alternateName VU University Medical Center
    183 schema:name Department Oncology, Free University Hospital, P.O. Box 7057, 1007, Amsterdam, MB, The Netherlands
    184 rdf:type schema:Organization
     




    Preview window. Press ESC to close (or click here)


    ...