Effect of nutrient deficiency on accumulation and relative molecular weight of poly-β-hydroxybutyric acid by methylotrophic bacterium, Pseudomonas 135 View Full Text


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Article Info

DATE

1992-09

AUTHORS

M. Daniel, J. H. Choi, J. H. Kim, J. M. Lebeault

ABSTRACT

Pseudomonas 135, a facultative methylotroph, was cultivated on methanol as a sole carbon and energy source for the accumulation of poly-β-hydroxybutyric acid (PHB). The cells grew fairly well on minimal synthetic medium containing 0.5% (v/v) of methanol at pH 7.0 and 30° C. The maximum specific growth rate was determined to be 0.26–0.28 h−1 with a growth yield of 0.38 in the optimized growth medium. For stimulation of PHB accumulation in the cells, deficiency of nutrients such as NHinf4sup+, Mg2+ and POinf4sup3−was crucial even though cell growth was significantly suppressed. The PHB content of a 40-h culture was determined to be 37% of the total cell mass in NHinf4sup+-limited medium, 42.5% on Mg2+-deficient medium, and 34.5% on POinf4sup3−-deficient medium. The maximum content of PHB in the cells could reach 55% in NHinf4sup+-limited fed-batch culture. The average relative molecular eight determined by gel permeation chromatography was 3.7 × 105 in NHinf4sup+-limited culture, 2.5 × 105 in Mg2+-deficientmedium, and 3.1 × 105 in POinf4sup3−-deficient medium. Polydispersity determined in each culture was relatively high (about 10–11). The solid PHB had a melting temperature of 173° C. More... »

PAGES

702-706

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf00174831

DOI

http://dx.doi.org/10.1007/bf00174831

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1046578221


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