Large-scale production of anthocyanin by Aralia cordata cell suspension cultures View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1993-11

AUTHORS

Yoshinori Kobayashi, Motomu Akita, Kazuo Sakamoto, Hongfeng Liu, Takeo Shigeoka, Takashi Koyano, Michio Kawamura, Tsutomu Furuya

ABSTRACT

The suspension culture of high anthocyanin-producing Aralia cordata cell lines, which grow and produce anthocyanin without light irradation, was scaled up from flasks to a 10-1 glass jar fermentor, a 95-1 stainless steel jar fermentor, and finally a 500-l pilot-scale jar fermentor. By the administration of CO2, cell damage was completely prevented and the anthocyanin content was kept as high as 7.0–17.2% (w/w) of the dried cells. In one of the operations of the 500-l jar fermentor, cells were cultivated for 16 days. During this operation, cell mass was increased by more than 26 times (cell yield: 69.2 kg fresh wt.) and the amount of anthocyanin increased by more than 55 times (anthocyanin yield: 545 g, anthocyanin content: 17.2% of the dried cells). More... »

PAGES

215-218

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf00170369

DOI

http://dx.doi.org/10.1007/bf00170369

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1022743487


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