Effect of pore size and shape on the immobilization of coffee (Coffea arabica L.) cells in porous matrices View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1992-01

AUTHORS

Kenji Koge, Yutaka Orihara, Tsutomu Furuya

ABSTRACT

The pore size and shape of porous matrices were evaluated as to their effect on the immobilization efficiency in cultured coffee (Coffea arabica L.)/cells. A hydrophilic porous matrix (13–20 pores/25 mm) and reticulate polyurethane foam (30 pores/25 mm) indicated more efficient immobilization than the others, in small cubes (1 cm3 × 9) and a strip (1 × 1 × 9 cm3) at the end of the fourth subculture. Among the large cubes (9 cm3), the reticulate one with the largest pore size (13 pores/25 mm) was the most advantageous for immobilization. In the strip-shaped matrices (1 × 1 × 9 cm3), immobilization was the most efficient in spite of its lower surface area as compared to the small cubes, except for those with the largest pore size. The strip-shaped foams, which were fixed on the inside of the flask against shaking, were effective for immobilization. Finally, strips (30 pores/25 mm) with slits to increase the surface area of the foam immobilized the largest amount of cells at the end of the fourth subculture. Caffeine production was not changed by diffenences in pore size. More... »

PAGES

452-455

Journal

TITLE

Applied Microbiology and Biotechnology

ISSUE

4

VOLUME

36

Author Affiliations

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf00170181

DOI

http://dx.doi.org/10.1007/bf00170181

DIMENSIONS

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