Induction of ajmalicine formation and related enzyme activities in Catharanthus roseus cells: effect of inoculum density View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1993-04

AUTHORS

Paulo R. H. Moreno, Juriaan E. Schlatmann, Robert van der Heijden, Walter M. van Gulik, Hens J. G. ten Hoopen, Robert Verpoorte, Joseph J. Heijnen

ABSTRACT

In Catharanthus roseus cell cultures the time courses of four enzyme activities, tryptophan decarboxylase (TDC), strictosidine synthase (SSS), geraniol-10-hydroxylase (G10H) and anthranilate synthase (AS), and alkaloid accumulation were compared under two different culture conditions (low-inoculum density and high-inoculum density on induction medium) and a control on growth medium. In growth medium a transient increase in TDC activity was first observed after which G10H reached its maximum activity; only tryptamine accumulated, no ajmalicine could be detected. Apparently, a concerted induction of enzyme activities is required for ajmalicine formation. Cells inoculated in induction medium showed such a concerted induction of AS, TDC and G10H activities. After 30 days the low-density culture had accumulated six times more ajmalicine (in mumoles/g) than the high-density culture. Thus, increase in biomass concentration (high-density cultures) did not enhance the total alkaloid production. The major differences observed in enzyme levels between high- and low-density cultures were in the AS and TDC activities, which were two to three times higher in the low-density culture, indicating that there is a positive correlation between ajmalicine formation and AS and TDC activities. More... »

PAGES

42-47

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf00166846

DOI

http://dx.doi.org/10.1007/bf00166846

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1044656504

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/7763550


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biochemistry and Cell Biology", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Anthranilate Synthase", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Anti-Inflammatory Agents, Non-Steroidal", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Antineoplastic Agents", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Aromatic-L-Amino-Acid Decarboxylases", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Carbon-Nitrogen Lyases", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Cells, Cultured", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Enzyme Induction", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Glucose", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Hydroxylation", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Mixed Function Oxygenases", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Plants, Medicinal", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Secologanin Tryptamine Alkaloids", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Terpenes", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Transferases", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Tryptamines", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Yohimbine", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Leiden University", 
          "id": "https://www.grid.ac/institutes/grid.5132.5", 
          "name": [
            "Division of Pharmacognosy, Center for Bio-Pharmaceutical Sciences, Leiden University, P. O. Box 9502, 2300 RA, Leiden, The Netherlands"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Moreno", 
        "givenName": "Paulo R. H.", 
        "id": "sg:person.012410556612.74", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.012410556612.74"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Delft University of Technology", 
          "id": "https://www.grid.ac/institutes/grid.5292.c", 
          "name": [
            "Department of Biochemical Engineering, Delft University of Technology, Julianalaan 67, 2628 BC, Delft, The Netherlands"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Schlatmann", 
        "givenName": "Juriaan E.", 
        "id": "sg:person.01333741152.73", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01333741152.73"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Leiden University", 
          "id": "https://www.grid.ac/institutes/grid.5132.5", 
          "name": [
            "Division of Pharmacognosy, Center for Bio-Pharmaceutical Sciences, Leiden University, P. O. Box 9502, 2300 RA, Leiden, The Netherlands"
          ], 
          "type": "Organization"
        }, 
        "familyName": "van der Heijden", 
        "givenName": "Robert", 
        "id": "sg:person.01103551450.51", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01103551450.51"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Delft University of Technology", 
          "id": "https://www.grid.ac/institutes/grid.5292.c", 
          "name": [
            "Department of Biochemical Engineering, Delft University of Technology, Julianalaan 67, 2628 BC, Delft, The Netherlands"
          ], 
          "type": "Organization"
        }, 
        "familyName": "van Gulik", 
        "givenName": "Walter M.", 
        "id": "sg:person.0615715153.53", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0615715153.53"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Delft University of Technology", 
          "id": "https://www.grid.ac/institutes/grid.5292.c", 
          "name": [
            "Department of Biochemical Engineering, Delft University of Technology, Julianalaan 67, 2628 BC, Delft, The Netherlands"
          ], 
          "type": "Organization"
        }, 
        "familyName": "ten Hoopen", 
        "givenName": "Hens J. G.", 
        "id": "sg:person.014516252143.03", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.014516252143.03"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Leiden University", 
          "id": "https://www.grid.ac/institutes/grid.5132.5", 
          "name": [
            "Division of Pharmacognosy, Center for Bio-Pharmaceutical Sciences, Leiden University, P. O. Box 9502, 2300 RA, Leiden, The Netherlands"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Verpoorte", 
        "givenName": "Robert", 
        "id": "sg:person.0607764165.99", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0607764165.99"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Delft University of Technology", 
          "id": "https://www.grid.ac/institutes/grid.5292.c", 
          "name": [
            "Department of Biochemical Engineering, Delft University of Technology, Julianalaan 67, 2628 BC, Delft, The Netherlands"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Heijnen", 
        "givenName": "Joseph J.", 
        "id": "sg:person.01026476151.36", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01026476151.36"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.1002/bit.260400802", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1007048730"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/978-3-642-66646-9_3", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1008453412", 
          "https://doi.org/10.1007/978-3-642-66646-9_3"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0304-4211(80)90025-5", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1011645895"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0304-4211(80)90025-5", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1011645895"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00047714", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1014387362", 
          "https://doi.org/10.1007/bf00047714"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00047714", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1014387362", 
          "https://doi.org/10.1007/bf00047714"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00269294", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1014807110", 
          "https://doi.org/10.1007/bf00269294"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00269294", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1014807110", 
          "https://doi.org/10.1007/bf00269294"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1399-3054.1965.tb06874.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1016020981"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00047715", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1017765877", 
          "https://doi.org/10.1007/bf00047715"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00047715", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1017765877", 
          "https://doi.org/10.1007/bf00047715"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0031-9422(91)83443-o", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1020639097"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/bit.260300805", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1021300149"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0021-9673(01)88638-6", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1021528016"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00263851", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1025987725", 
          "https://doi.org/10.1007/bf00263851"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00263851", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1025987725", 
          "https://doi.org/10.1007/bf00263851"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0003-2697(87)90210-7", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1038610483"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00269710", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1043211970", 
          "https://doi.org/10.1007/bf00269710"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00269710", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1043211970", 
          "https://doi.org/10.1007/bf00269710"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0003-2697(77)90043-4", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1043533757"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00172730", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1051339349", 
          "https://doi.org/10.1007/bf00172730"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00172730", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1051339349", 
          "https://doi.org/10.1007/bf00172730"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0003-2697(89)90333-3", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1053003836"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00391274", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1053190604", 
          "https://doi.org/10.1007/bf00391274"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00391274", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1053190604", 
          "https://doi.org/10.1007/bf00391274"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1021/bi00650a023", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1055184043"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1055/s-2006-961445", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1057473354"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1055/s-2006-962739", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1057474625"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1515/znc-1980-7-805", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1104148920"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1515/znc-1987-9-1012", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1104149631"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1515/znc-1981-1-209", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1104149741"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1515/znc-1987-9-1017", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1104150940"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "1993-04", 
    "datePublishedReg": "1993-04-01", 
    "description": "In Catharanthus roseus cell cultures the time courses of four enzyme activities, tryptophan decarboxylase (TDC), strictosidine synthase (SSS), geraniol-10-hydroxylase (G10H) and anthranilate synthase (AS), and alkaloid accumulation were compared under two different culture conditions (low-inoculum density and high-inoculum density on induction medium) and a control on growth medium. In growth medium a transient increase in TDC activity was first observed after which G10H reached its maximum activity; only tryptamine accumulated, no ajmalicine could be detected. Apparently, a concerted induction of enzyme activities is required for ajmalicine formation. Cells inoculated in induction medium showed such a concerted induction of AS, TDC and G10H activities. After 30 days the low-density culture had accumulated six times more ajmalicine (in mumoles/g) than the high-density culture. Thus, increase in biomass concentration (high-density cultures) did not enhance the total alkaloid production. The major differences observed in enzyme levels between high- and low-density cultures were in the AS and TDC activities, which were two to three times higher in the low-density culture, indicating that there is a positive correlation between ajmalicine formation and AS and TDC activities.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1007/bf00166846", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": false, 
    "isPartOf": [
      {
        "id": "sg:journal.1083533", 
        "issn": [
          "0175-7598", 
          "1432-0614"
        ], 
        "name": "Applied Microbiology and Biotechnology", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "1", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "39"
      }
    ], 
    "name": "Induction of ajmalicine formation and related enzyme activities in Catharanthus roseus cells: effect of inoculum density", 
    "pagination": "42-47", 
    "productId": [
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1007/bf00166846"
        ]
      }, 
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "d4b74e293ba4468e2827350af2e4effc72b89f48e595f036360abdc44ab9bfaf"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1044656504"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "8406612"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "7763550"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1007/bf00166846", 
      "https://app.dimensions.ai/details/publication/pub.1044656504"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-15T08:48", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000374_0000000374/records_119714_00000001.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "http://link.springer.com/10.1007/BF00166846"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/bf00166846'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/bf00166846'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/bf00166846'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/bf00166846'


 

This table displays all metadata directly associated to this object as RDF triples.

258 TRIPLES      21 PREDICATES      69 URIs      37 LITERALS      25 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1007/bf00166846 schema:about N1b455220a53443d7a10e419b3dc2c8c8
2 N32acbb3a897441839f520abb595ed2b0
3 N501a0b18805b48ce8780c8a507c0335b
4 N5eadfe81783d4563a4142c4c55306e81
5 N770e246245dd4a5f80f63b4476e1a667
6 N776f4fa9181d4e5485c0500b767c3e16
7 N9d84e2093e674ebabf66adfb448b6edf
8 Na552046a14934c4085d172c3f6a6f310
9 Na553a005ab794e70a84a166dd599ab10
10 Nb16e6419d862472681ee1f97fb9089df
11 Nbefe43c5695f480b8ee7c987ecc926c2
12 Nc225802f70044fe981767f4d5a1f3755
13 Nc4a9256c72034f1aa0c7e63c9c3ea1aa
14 Nc8589878d9cb45beb0115b17739331ea
15 Ndce8ce8a976e4d428c79401133c99ea3
16 Ne19cd1e083c140aab70eae725bbd7b9d
17 anzsrc-for:06
18 anzsrc-for:0601
19 schema:author Nc62152d8d54f47e5a6d4323817d1deb9
20 schema:citation sg:pub.10.1007/978-3-642-66646-9_3
21 sg:pub.10.1007/bf00047714
22 sg:pub.10.1007/bf00047715
23 sg:pub.10.1007/bf00172730
24 sg:pub.10.1007/bf00263851
25 sg:pub.10.1007/bf00269294
26 sg:pub.10.1007/bf00269710
27 sg:pub.10.1007/bf00391274
28 https://doi.org/10.1002/bit.260300805
29 https://doi.org/10.1002/bit.260400802
30 https://doi.org/10.1016/0003-2697(77)90043-4
31 https://doi.org/10.1016/0003-2697(87)90210-7
32 https://doi.org/10.1016/0003-2697(89)90333-3
33 https://doi.org/10.1016/0031-9422(91)83443-o
34 https://doi.org/10.1016/0304-4211(80)90025-5
35 https://doi.org/10.1016/s0021-9673(01)88638-6
36 https://doi.org/10.1021/bi00650a023
37 https://doi.org/10.1055/s-2006-961445
38 https://doi.org/10.1055/s-2006-962739
39 https://doi.org/10.1111/j.1399-3054.1965.tb06874.x
40 https://doi.org/10.1515/znc-1980-7-805
41 https://doi.org/10.1515/znc-1981-1-209
42 https://doi.org/10.1515/znc-1987-9-1012
43 https://doi.org/10.1515/znc-1987-9-1017
44 schema:datePublished 1993-04
45 schema:datePublishedReg 1993-04-01
46 schema:description In Catharanthus roseus cell cultures the time courses of four enzyme activities, tryptophan decarboxylase (TDC), strictosidine synthase (SSS), geraniol-10-hydroxylase (G10H) and anthranilate synthase (AS), and alkaloid accumulation were compared under two different culture conditions (low-inoculum density and high-inoculum density on induction medium) and a control on growth medium. In growth medium a transient increase in TDC activity was first observed after which G10H reached its maximum activity; only tryptamine accumulated, no ajmalicine could be detected. Apparently, a concerted induction of enzyme activities is required for ajmalicine formation. Cells inoculated in induction medium showed such a concerted induction of AS, TDC and G10H activities. After 30 days the low-density culture had accumulated six times more ajmalicine (in mumoles/g) than the high-density culture. Thus, increase in biomass concentration (high-density cultures) did not enhance the total alkaloid production. The major differences observed in enzyme levels between high- and low-density cultures were in the AS and TDC activities, which were two to three times higher in the low-density culture, indicating that there is a positive correlation between ajmalicine formation and AS and TDC activities.
47 schema:genre research_article
48 schema:inLanguage en
49 schema:isAccessibleForFree false
50 schema:isPartOf Nd29e6d5282204eb7b4657c94c2807f16
51 Nf4621d04c6f24a329e2b4fba9551fcea
52 sg:journal.1083533
53 schema:name Induction of ajmalicine formation and related enzyme activities in Catharanthus roseus cells: effect of inoculum density
54 schema:pagination 42-47
55 schema:productId N4c79af79b2da41cd85b131a25582e44e
56 N6f1d1f213b924a2fa5885707b8590399
57 Nae4f440d0bc54fab98320a89ef662676
58 Nc9ef4c16aab44efa98ff7e9ebdcbb268
59 Nd278331214ad4c37810f529eb73d6d3f
60 schema:sameAs https://app.dimensions.ai/details/publication/pub.1044656504
61 https://doi.org/10.1007/bf00166846
62 schema:sdDatePublished 2019-04-15T08:48
63 schema:sdLicense https://scigraph.springernature.com/explorer/license/
64 schema:sdPublisher Naa5255c126594985aaa1165e30bd91bc
65 schema:url http://link.springer.com/10.1007/BF00166846
66 sgo:license sg:explorer/license/
67 sgo:sdDataset articles
68 rdf:type schema:ScholarlyArticle
69 N11596fe7c97549509c25701cc500d831 rdf:first sg:person.01333741152.73
70 rdf:rest N5e2f729a7e734fe9af0bbed2715a0b30
71 N1676750bf2bc430aad180d2d57e3600e rdf:first sg:person.014516252143.03
72 rdf:rest N2c664aca785d497da80a372cedd212c7
73 N1b455220a53443d7a10e419b3dc2c8c8 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
74 schema:name Antineoplastic Agents
75 rdf:type schema:DefinedTerm
76 N2c664aca785d497da80a372cedd212c7 rdf:first sg:person.0607764165.99
77 rdf:rest N5b617fb328e7470ca13d9701a4c79ff3
78 N32acbb3a897441839f520abb595ed2b0 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
79 schema:name Mixed Function Oxygenases
80 rdf:type schema:DefinedTerm
81 N4c79af79b2da41cd85b131a25582e44e schema:name dimensions_id
82 schema:value pub.1044656504
83 rdf:type schema:PropertyValue
84 N501a0b18805b48ce8780c8a507c0335b schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
85 schema:name Tryptamines
86 rdf:type schema:DefinedTerm
87 N5b617fb328e7470ca13d9701a4c79ff3 rdf:first sg:person.01026476151.36
88 rdf:rest rdf:nil
89 N5e2f729a7e734fe9af0bbed2715a0b30 rdf:first sg:person.01103551450.51
90 rdf:rest N81c627066bbf46069908bc5e8b51bdff
91 N5eadfe81783d4563a4142c4c55306e81 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
92 schema:name Carbon-Nitrogen Lyases
93 rdf:type schema:DefinedTerm
94 N6f1d1f213b924a2fa5885707b8590399 schema:name readcube_id
95 schema:value d4b74e293ba4468e2827350af2e4effc72b89f48e595f036360abdc44ab9bfaf
96 rdf:type schema:PropertyValue
97 N770e246245dd4a5f80f63b4476e1a667 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
98 schema:name Enzyme Induction
99 rdf:type schema:DefinedTerm
100 N776f4fa9181d4e5485c0500b767c3e16 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
101 schema:name Hydroxylation
102 rdf:type schema:DefinedTerm
103 N81c627066bbf46069908bc5e8b51bdff rdf:first sg:person.0615715153.53
104 rdf:rest N1676750bf2bc430aad180d2d57e3600e
105 N9d84e2093e674ebabf66adfb448b6edf schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
106 schema:name Glucose
107 rdf:type schema:DefinedTerm
108 Na552046a14934c4085d172c3f6a6f310 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
109 schema:name Anthranilate Synthase
110 rdf:type schema:DefinedTerm
111 Na553a005ab794e70a84a166dd599ab10 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
112 schema:name Secologanin Tryptamine Alkaloids
113 rdf:type schema:DefinedTerm
114 Naa5255c126594985aaa1165e30bd91bc schema:name Springer Nature - SN SciGraph project
115 rdf:type schema:Organization
116 Nae4f440d0bc54fab98320a89ef662676 schema:name nlm_unique_id
117 schema:value 8406612
118 rdf:type schema:PropertyValue
119 Nb16e6419d862472681ee1f97fb9089df schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
120 schema:name Terpenes
121 rdf:type schema:DefinedTerm
122 Nbefe43c5695f480b8ee7c987ecc926c2 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
123 schema:name Yohimbine
124 rdf:type schema:DefinedTerm
125 Nc225802f70044fe981767f4d5a1f3755 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
126 schema:name Aromatic-L-Amino-Acid Decarboxylases
127 rdf:type schema:DefinedTerm
128 Nc4a9256c72034f1aa0c7e63c9c3ea1aa schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
129 schema:name Transferases
130 rdf:type schema:DefinedTerm
131 Nc62152d8d54f47e5a6d4323817d1deb9 rdf:first sg:person.012410556612.74
132 rdf:rest N11596fe7c97549509c25701cc500d831
133 Nc8589878d9cb45beb0115b17739331ea schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
134 schema:name Cells, Cultured
135 rdf:type schema:DefinedTerm
136 Nc9ef4c16aab44efa98ff7e9ebdcbb268 schema:name pubmed_id
137 schema:value 7763550
138 rdf:type schema:PropertyValue
139 Nd278331214ad4c37810f529eb73d6d3f schema:name doi
140 schema:value 10.1007/bf00166846
141 rdf:type schema:PropertyValue
142 Nd29e6d5282204eb7b4657c94c2807f16 schema:issueNumber 1
143 rdf:type schema:PublicationIssue
144 Ndce8ce8a976e4d428c79401133c99ea3 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
145 schema:name Anti-Inflammatory Agents, Non-Steroidal
146 rdf:type schema:DefinedTerm
147 Ne19cd1e083c140aab70eae725bbd7b9d schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
148 schema:name Plants, Medicinal
149 rdf:type schema:DefinedTerm
150 Nf4621d04c6f24a329e2b4fba9551fcea schema:volumeNumber 39
151 rdf:type schema:PublicationVolume
152 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
153 schema:name Biological Sciences
154 rdf:type schema:DefinedTerm
155 anzsrc-for:0601 schema:inDefinedTermSet anzsrc-for:
156 schema:name Biochemistry and Cell Biology
157 rdf:type schema:DefinedTerm
158 sg:journal.1083533 schema:issn 0175-7598
159 1432-0614
160 schema:name Applied Microbiology and Biotechnology
161 rdf:type schema:Periodical
162 sg:person.01026476151.36 schema:affiliation https://www.grid.ac/institutes/grid.5292.c
163 schema:familyName Heijnen
164 schema:givenName Joseph J.
165 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01026476151.36
166 rdf:type schema:Person
167 sg:person.01103551450.51 schema:affiliation https://www.grid.ac/institutes/grid.5132.5
168 schema:familyName van der Heijden
169 schema:givenName Robert
170 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01103551450.51
171 rdf:type schema:Person
172 sg:person.012410556612.74 schema:affiliation https://www.grid.ac/institutes/grid.5132.5
173 schema:familyName Moreno
174 schema:givenName Paulo R. H.
175 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.012410556612.74
176 rdf:type schema:Person
177 sg:person.01333741152.73 schema:affiliation https://www.grid.ac/institutes/grid.5292.c
178 schema:familyName Schlatmann
179 schema:givenName Juriaan E.
180 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01333741152.73
181 rdf:type schema:Person
182 sg:person.014516252143.03 schema:affiliation https://www.grid.ac/institutes/grid.5292.c
183 schema:familyName ten Hoopen
184 schema:givenName Hens J. G.
185 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.014516252143.03
186 rdf:type schema:Person
187 sg:person.0607764165.99 schema:affiliation https://www.grid.ac/institutes/grid.5132.5
188 schema:familyName Verpoorte
189 schema:givenName Robert
190 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0607764165.99
191 rdf:type schema:Person
192 sg:person.0615715153.53 schema:affiliation https://www.grid.ac/institutes/grid.5292.c
193 schema:familyName van Gulik
194 schema:givenName Walter M.
195 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0615715153.53
196 rdf:type schema:Person
197 sg:pub.10.1007/978-3-642-66646-9_3 schema:sameAs https://app.dimensions.ai/details/publication/pub.1008453412
198 https://doi.org/10.1007/978-3-642-66646-9_3
199 rdf:type schema:CreativeWork
200 sg:pub.10.1007/bf00047714 schema:sameAs https://app.dimensions.ai/details/publication/pub.1014387362
201 https://doi.org/10.1007/bf00047714
202 rdf:type schema:CreativeWork
203 sg:pub.10.1007/bf00047715 schema:sameAs https://app.dimensions.ai/details/publication/pub.1017765877
204 https://doi.org/10.1007/bf00047715
205 rdf:type schema:CreativeWork
206 sg:pub.10.1007/bf00172730 schema:sameAs https://app.dimensions.ai/details/publication/pub.1051339349
207 https://doi.org/10.1007/bf00172730
208 rdf:type schema:CreativeWork
209 sg:pub.10.1007/bf00263851 schema:sameAs https://app.dimensions.ai/details/publication/pub.1025987725
210 https://doi.org/10.1007/bf00263851
211 rdf:type schema:CreativeWork
212 sg:pub.10.1007/bf00269294 schema:sameAs https://app.dimensions.ai/details/publication/pub.1014807110
213 https://doi.org/10.1007/bf00269294
214 rdf:type schema:CreativeWork
215 sg:pub.10.1007/bf00269710 schema:sameAs https://app.dimensions.ai/details/publication/pub.1043211970
216 https://doi.org/10.1007/bf00269710
217 rdf:type schema:CreativeWork
218 sg:pub.10.1007/bf00391274 schema:sameAs https://app.dimensions.ai/details/publication/pub.1053190604
219 https://doi.org/10.1007/bf00391274
220 rdf:type schema:CreativeWork
221 https://doi.org/10.1002/bit.260300805 schema:sameAs https://app.dimensions.ai/details/publication/pub.1021300149
222 rdf:type schema:CreativeWork
223 https://doi.org/10.1002/bit.260400802 schema:sameAs https://app.dimensions.ai/details/publication/pub.1007048730
224 rdf:type schema:CreativeWork
225 https://doi.org/10.1016/0003-2697(77)90043-4 schema:sameAs https://app.dimensions.ai/details/publication/pub.1043533757
226 rdf:type schema:CreativeWork
227 https://doi.org/10.1016/0003-2697(87)90210-7 schema:sameAs https://app.dimensions.ai/details/publication/pub.1038610483
228 rdf:type schema:CreativeWork
229 https://doi.org/10.1016/0003-2697(89)90333-3 schema:sameAs https://app.dimensions.ai/details/publication/pub.1053003836
230 rdf:type schema:CreativeWork
231 https://doi.org/10.1016/0031-9422(91)83443-o schema:sameAs https://app.dimensions.ai/details/publication/pub.1020639097
232 rdf:type schema:CreativeWork
233 https://doi.org/10.1016/0304-4211(80)90025-5 schema:sameAs https://app.dimensions.ai/details/publication/pub.1011645895
234 rdf:type schema:CreativeWork
235 https://doi.org/10.1016/s0021-9673(01)88638-6 schema:sameAs https://app.dimensions.ai/details/publication/pub.1021528016
236 rdf:type schema:CreativeWork
237 https://doi.org/10.1021/bi00650a023 schema:sameAs https://app.dimensions.ai/details/publication/pub.1055184043
238 rdf:type schema:CreativeWork
239 https://doi.org/10.1055/s-2006-961445 schema:sameAs https://app.dimensions.ai/details/publication/pub.1057473354
240 rdf:type schema:CreativeWork
241 https://doi.org/10.1055/s-2006-962739 schema:sameAs https://app.dimensions.ai/details/publication/pub.1057474625
242 rdf:type schema:CreativeWork
243 https://doi.org/10.1111/j.1399-3054.1965.tb06874.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1016020981
244 rdf:type schema:CreativeWork
245 https://doi.org/10.1515/znc-1980-7-805 schema:sameAs https://app.dimensions.ai/details/publication/pub.1104148920
246 rdf:type schema:CreativeWork
247 https://doi.org/10.1515/znc-1981-1-209 schema:sameAs https://app.dimensions.ai/details/publication/pub.1104149741
248 rdf:type schema:CreativeWork
249 https://doi.org/10.1515/znc-1987-9-1012 schema:sameAs https://app.dimensions.ai/details/publication/pub.1104149631
250 rdf:type schema:CreativeWork
251 https://doi.org/10.1515/znc-1987-9-1017 schema:sameAs https://app.dimensions.ai/details/publication/pub.1104150940
252 rdf:type schema:CreativeWork
253 https://www.grid.ac/institutes/grid.5132.5 schema:alternateName Leiden University
254 schema:name Division of Pharmacognosy, Center for Bio-Pharmaceutical Sciences, Leiden University, P. O. Box 9502, 2300 RA, Leiden, The Netherlands
255 rdf:type schema:Organization
256 https://www.grid.ac/institutes/grid.5292.c schema:alternateName Delft University of Technology
257 schema:name Department of Biochemical Engineering, Delft University of Technology, Julianalaan 67, 2628 BC, Delft, The Netherlands
258 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...