Degradation of 2-chloroethanol by free and immobilized Pseudomonas putida US 2 View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1995-12

AUTHORS

M. Knippschild, H. -J. Rehm

ABSTRACT

The degradation of 2-chloroethanol by Pseudomonas putida US 2 was investigated in shaking flasks, air-bubble columns and packed-bed fermenters by free cells, calcium-alginate-entrapped cells and on cells on granular clay adsorbed. Entrapped cells tolerated increasing concentrations of 2-chloroethanol better than free cells. Their maximum degradative activity could be observed at 34°C and pH 7.0. The degradation of 2-chloroethanol leads to a decrease of pH and to a stagnation of mineralization, particularly with free or entrapped cells. Following the stabilization of pH, supplementation with succinate resulted in a complete degradation of higher 2-chloroethanol concentrations. Less 2-chloroethanol was degraded in air-bubble columns and larger amounts in packed-bed fermenters. 2-Chloroethanol was mineralized faster by free or entrapped P. putida US 2 than by adsorbed cells, which, on the other hand, were able to remove higher concentrations of the compound. The results with P. putida US 2 are a good indication that this microorganism could be used in waste-water treatment and soil-decontamination systems. More... »

PAGES

253-258

References to SciGraph publications

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf00164511

DOI

http://dx.doi.org/10.1007/bf00164511

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1029080600


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