Ontology type: schema:ScholarlyArticle
1995-07
AUTHORSTeresita Díaz, Ulf St»hl, Francisco Batista-Viera, Jan Carlsson
ABSTRACTPullulanase was provided with up to nine “de novo” thiol groups through a two-step procedure without substantially affecting its enzymatic activity. The chemically modified enzyme was immobilized via disulfide bond formation on two kinds of thiolreactive gels (pyridyldisulfide- and thiolsulfonate-substituted agarose). Thiolation of pullulanase improved both the immobilization yield and the apparent specific activity of the derivatives. More... »
PAGES533-538
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DOIhttp://dx.doi.org/10.1007/bf00159572
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