Modification of the bi-directional sliding movement of actin filaments along native thick filaments isolated from a clam View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1996-12

AUTHORS

Jan M. West, Hideo Higuchi, Akihiko Ishijima, Toshio Yanagida

ABSTRACT

The properties of bi-directional sliding of F-actin prepared from rabbit skeletal muscle moving along clam thick filaments have been characterized in the presence of agents known to modify unloaded shortening velocity in muscle to determine if the sliding characteristics of actin are similar in the two directions of movement. Actin filaments moved at a fast velocity towards the central bare zone (11.1±0.2 μm s-1) and at a slower velocity away from the bare zone (3.9±0.3 μm s-1). Movement of filaments at the slow sliding velocity is thought to be sustained by a change in orientation of the myosin head. The Michaelis Menten constant (Km values) of ∼0.3 mm in the presence of MgATP concentrations of 0.01–2.0 mm at an ionic strength of 43.5 mm were reduced to ∼0.1 mm at low ionic strength (18.5 mm) although the Km values at the fast and slow sliding velocities at each ionic strength were similar. In the presence of constant concentrations of MgATP, increasing the MgADP concentrations from 0.5 to 2 mm, decreased the bi-directional sliding velocity of actin. The data were well fitted with an equation described by Michaelis Menten kinetics yielding mean absolute Km and Ki values of 0.41±0.01 and 0.44±0.05 mm for the fast velocity and 0.29±0.07 and 0.45±0.02 mm for the slow velocity of sliding, respectively. The Km and Ki values were not significantly different from each other at either the fast or slow sliding, velocities. The actin filament sliding velocity appeared to be controlled through the thick filament as actin was devoid of regulatory proteins and the presence of Ca2+ modified the MgATP dependent movement of actin. The pCa value for half maximal sliding velocity was 7.0 for both fast and slow velocities. The Km and Ki values and the Ca2+ sensitivity of the actin movement at the fast and slow sliding velocity are similar suggesting that no major biochemical changes have occurred in the myosin head as a result of a change in orientation. More... »

PAGES

637-646

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf00154058

DOI

http://dx.doi.org/10.1007/bf00154058

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1013437230

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/8994083


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0601", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biochemistry and Cell Biology", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0606", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Physiology", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Actins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Adenosine Diphosphate", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Adenosine Triphosphate", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Animals", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Bivalvia", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Calcium", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Dose-Response Relationship, Drug", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Glass", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Magnesium", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Muscles", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Myosins", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Rabbits", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Rotation", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "Department of Biophysical Engineering, Osaka University, Toyonaka, Osaka, Japan", 
          "id": "http://www.grid.ac/institutes/grid.136593.b", 
          "name": [
            "Department of Biophysical Engineering, Osaka University, Toyonaka, Osaka, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "West", 
        "givenName": "Jan M.", 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Yanagida Biomotron Project, 2-4-14 Senba-Higashi, 562, Mino, Osaka, Japan", 
          "id": "http://www.grid.ac/institutes/None", 
          "name": [
            "Yanagida Biomotron Project, 2-4-14 Senba-Higashi, 562, Mino, Osaka, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Higuchi", 
        "givenName": "Hideo", 
        "id": "sg:person.015604164731.98", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.015604164731.98"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Wako Research Centre, Honda R & D Co. Ltd, Wako, Saitama, Japan", 
          "id": "http://www.grid.ac/institutes/grid.471052.5", 
          "name": [
            "Yanagida Biomotron Project, 2-4-14 Senba-Higashi, 562, Mino, Osaka, Japan", 
            "Wako Research Centre, Honda R & D Co. Ltd, Wako, Saitama, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Ishijima", 
        "givenName": "Akihiko", 
        "id": "sg:person.01160232013.39", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01160232013.39"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "Yanagida Biomotron Project, 2-4-14 Senba-Higashi, 562, Mino, Osaka, Japan", 
          "id": "http://www.grid.ac/institutes/None", 
          "name": [
            "Department of Biophysical Engineering, Osaka University, Toyonaka, Osaka, Japan", 
            "Yanagida Biomotron Project, 2-4-14 Senba-Higashi, 562, Mino, Osaka, Japan"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Yanagida", 
        "givenName": "Toshio", 
        "id": "sg:person.015141357621.93", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.015141357621.93"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "sg:pub.10.1038/368306a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1010508328", 
          "https://doi.org/10.1038/368306a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/nsb0494-209", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1014009654", 
          "https://doi.org/10.1038/nsb0494-209"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/341154a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1013597084", 
          "https://doi.org/10.1038/341154a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/289690a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1041755363", 
          "https://doi.org/10.1038/289690a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/339481a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1034823717", 
          "https://doi.org/10.1038/339481a0"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "1996-12", 
    "datePublishedReg": "1996-12-01", 
    "description": "The properties of bi-directional sliding of F-actin prepared from rabbit skeletal muscle moving along clam thick filaments have been characterized in the presence of agents known to modify unloaded shortening velocity in muscle to determine if the sliding characteristics of actin are similar in the two directions of movement. Actin filaments moved at a fast velocity towards the central bare zone (11.1\u00b10.2 \u03bcm s-1) and at a slower velocity away from the bare zone (3.9\u00b10.3 \u03bcm s-1). Movement of filaments at the slow sliding velocity is thought to be sustained by a change in orientation of the myosin head. The Michaelis Menten constant (Km values) of \u223c0.3 mm in the presence of MgATP concentrations of 0.01\u20132.0 mm at an ionic strength of 43.5 mm were reduced to \u223c0.1 mm at low ionic strength (18.5 mm) although the Km values at the fast and slow sliding velocities at each ionic strength were similar. In the presence of constant concentrations of MgATP, increasing the MgADP concentrations from 0.5 to 2 mm, decreased the bi-directional sliding velocity of actin. The data were well fitted with an equation described by Michaelis Menten kinetics yielding mean absolute Km and Ki values of 0.41\u00b10.01 and 0.44\u00b10.05 mm for the fast velocity and 0.29\u00b10.07 and 0.45\u00b10.02 mm for the slow velocity of sliding, respectively. The Km and Ki values were not significantly different from each other at either the fast or slow sliding, velocities. The actin filament sliding velocity appeared to be controlled through the thick filament as actin was devoid of regulatory proteins and the presence of Ca2+ modified the MgATP dependent movement of actin. The pCa value for half maximal sliding velocity was 7.0 for both fast and slow velocities. The Km and Ki values and the Ca2+ sensitivity of the actin movement at the fast and slow sliding velocity are similar suggesting that no major biochemical changes have occurred in the myosin head as a result of a change in orientation.", 
    "genre": "article", 
    "id": "sg:pub.10.1007/bf00154058", 
    "isAccessibleForFree": false, 
    "isPartOf": [
      {
        "id": "sg:journal.1091334", 
        "issn": [
          "0142-4319", 
          "1573-2657"
        ], 
        "name": "Journal of Muscle Research and Cell Motility", 
        "publisher": "Springer Nature", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "6", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "17"
      }
    ], 
    "keywords": [
      "sliding velocity", 
      "fast velocity", 
      "slow velocity", 
      "velocity", 
      "slow sliding velocities", 
      "movement of filaments", 
      "equations", 
      "slow sliding", 
      "dependent movement", 
      "sliding movement", 
      "direction of movement", 
      "Menten", 
      "Michaelis-Menten", 
      "filaments", 
      "sliding", 
      "values", 
      "properties", 
      "orientation", 
      "direction", 
      "Menten kinetics", 
      "strength", 
      "Michaelis-Menten kinetics", 
      "results", 
      "presence", 
      "zone", 
      "central bare zone", 
      "constant concentration", 
      "data", 
      "movement", 
      "MgADP concentration", 
      "characteristics", 
      "modification", 
      "changes", 
      "myosin heads", 
      "sensitivity", 
      "actin filaments", 
      "kinetics", 
      "native thick filaments", 
      "actin movement", 
      "concentration", 
      "PCA values", 
      "presence of agents", 
      "head", 
      "ionic strength", 
      "thick filaments", 
      "shortening velocity", 
      "low ionic strength", 
      "Km", 
      "suggesting", 
      "agents", 
      "unloaded shortening velocity", 
      "bare zone", 
      "Ki values", 
      "major biochemical changes", 
      "skeletal muscle", 
      "biochemical changes", 
      "muscle", 
      "presence of Ca2", 
      "similar suggesting", 
      "rabbit skeletal muscle", 
      "actin", 
      "MgATP concentration", 
      "regulatory proteins", 
      "Ca2", 
      "F-actin", 
      "protein", 
      "Km values", 
      "MgATP", 
      "clams", 
      "bi-directional sliding", 
      "velocity of actin", 
      "characteristics of actin"
    ], 
    "name": "Modification of the bi-directional sliding movement of actin filaments along native thick filaments isolated from a clam", 
    "pagination": "637-646", 
    "productId": [
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1013437230"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1007/bf00154058"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "8994083"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1007/bf00154058", 
      "https://app.dimensions.ai/details/publication/pub.1013437230"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2022-12-01T06:21", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-springernature-scigraph/baseset/20221201/entities/gbq_results/article/article_282.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "https://doi.org/10.1007/bf00154058"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/bf00154058'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/bf00154058'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/bf00154058'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/bf00154058'


 

This table displays all metadata directly associated to this object as RDF triples.

237 TRIPLES      21 PREDICATES      117 URIs      103 LITERALS      20 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1007/bf00154058 schema:about N0b9b1a2fbbe348ea8803eebf0118cd12
2 N3a99b175c9d24412b35a1a64d07ccaee
3 N3efc975b9a2c46b9ad37e534e6ebe851
4 N4275151018534961b52566a831ec41b4
5 N44a70acb6aa94140a9db7ae51cb65420
6 N48be096fe38e43ccad8cdd923834a9a7
7 N533933361d6340a8a0e20bf9f3437447
8 N57f8e26f00324c44bf5e04e3afe4284f
9 N85b41c72d51d4cd3a59639f72f2d0bc6
10 Nb9473148012d4f25bfb53ff33cb89c68
11 Nd45ce61178424040a5708d1f08f2719c
12 Neea95eb1210947ebbb427647fe0035c0
13 Nff5e0e61b7f242d48af27219455975fc
14 anzsrc-for:06
15 anzsrc-for:0601
16 anzsrc-for:0606
17 schema:author Na7bf54b224e74dcd8dc9839c05ea7b16
18 schema:citation sg:pub.10.1038/289690a0
19 sg:pub.10.1038/339481a0
20 sg:pub.10.1038/341154a0
21 sg:pub.10.1038/368306a0
22 sg:pub.10.1038/nsb0494-209
23 schema:datePublished 1996-12
24 schema:datePublishedReg 1996-12-01
25 schema:description The properties of bi-directional sliding of F-actin prepared from rabbit skeletal muscle moving along clam thick filaments have been characterized in the presence of agents known to modify unloaded shortening velocity in muscle to determine if the sliding characteristics of actin are similar in the two directions of movement. Actin filaments moved at a fast velocity towards the central bare zone (11.1±0.2 μm s-1) and at a slower velocity away from the bare zone (3.9±0.3 μm s-1). Movement of filaments at the slow sliding velocity is thought to be sustained by a change in orientation of the myosin head. The Michaelis Menten constant (Km values) of ∼0.3 mm in the presence of MgATP concentrations of 0.01–2.0 mm at an ionic strength of 43.5 mm were reduced to ∼0.1 mm at low ionic strength (18.5 mm) although the Km values at the fast and slow sliding velocities at each ionic strength were similar. In the presence of constant concentrations of MgATP, increasing the MgADP concentrations from 0.5 to 2 mm, decreased the bi-directional sliding velocity of actin. The data were well fitted with an equation described by Michaelis Menten kinetics yielding mean absolute Km and Ki values of 0.41±0.01 and 0.44±0.05 mm for the fast velocity and 0.29±0.07 and 0.45±0.02 mm for the slow velocity of sliding, respectively. The Km and Ki values were not significantly different from each other at either the fast or slow sliding, velocities. The actin filament sliding velocity appeared to be controlled through the thick filament as actin was devoid of regulatory proteins and the presence of Ca2+ modified the MgATP dependent movement of actin. The pCa value for half maximal sliding velocity was 7.0 for both fast and slow velocities. The Km and Ki values and the Ca2+ sensitivity of the actin movement at the fast and slow sliding velocity are similar suggesting that no major biochemical changes have occurred in the myosin head as a result of a change in orientation.
26 schema:genre article
27 schema:isAccessibleForFree false
28 schema:isPartOf N30b3e754e4e34071a39d87d0338835af
29 N88a83f8c030c4000bbf443aca0ab1179
30 sg:journal.1091334
31 schema:keywords Ca2
32 F-actin
33 Ki values
34 Km
35 Km values
36 Menten
37 Menten kinetics
38 MgADP concentration
39 MgATP
40 MgATP concentration
41 Michaelis-Menten
42 Michaelis-Menten kinetics
43 PCA values
44 actin
45 actin filaments
46 actin movement
47 agents
48 bare zone
49 bi-directional sliding
50 biochemical changes
51 central bare zone
52 changes
53 characteristics
54 characteristics of actin
55 clams
56 concentration
57 constant concentration
58 data
59 dependent movement
60 direction
61 direction of movement
62 equations
63 fast velocity
64 filaments
65 head
66 ionic strength
67 kinetics
68 low ionic strength
69 major biochemical changes
70 modification
71 movement
72 movement of filaments
73 muscle
74 myosin heads
75 native thick filaments
76 orientation
77 presence
78 presence of Ca2
79 presence of agents
80 properties
81 protein
82 rabbit skeletal muscle
83 regulatory proteins
84 results
85 sensitivity
86 shortening velocity
87 similar suggesting
88 skeletal muscle
89 sliding
90 sliding movement
91 sliding velocity
92 slow sliding
93 slow sliding velocities
94 slow velocity
95 strength
96 suggesting
97 thick filaments
98 unloaded shortening velocity
99 values
100 velocity
101 velocity of actin
102 zone
103 schema:name Modification of the bi-directional sliding movement of actin filaments along native thick filaments isolated from a clam
104 schema:pagination 637-646
105 schema:productId N33fb45b1d1f94ae1a69f2c217c457c79
106 Nc9fd049292654101988f43f1b0ad0035
107 Nd5a89ca031314fb0adcf5fc0d7350f3e
108 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013437230
109 https://doi.org/10.1007/bf00154058
110 schema:sdDatePublished 2022-12-01T06:21
111 schema:sdLicense https://scigraph.springernature.com/explorer/license/
112 schema:sdPublisher Nac9c5c5cf8ff44689f0ff24391aa3447
113 schema:url https://doi.org/10.1007/bf00154058
114 sgo:license sg:explorer/license/
115 sgo:sdDataset articles
116 rdf:type schema:ScholarlyArticle
117 N0b9b1a2fbbe348ea8803eebf0118cd12 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
118 schema:name Adenosine Diphosphate
119 rdf:type schema:DefinedTerm
120 N0bf578bb24454e0c9e2d6dd4d89c9d41 rdf:first sg:person.015141357621.93
121 rdf:rest rdf:nil
122 N30b3e754e4e34071a39d87d0338835af schema:volumeNumber 17
123 rdf:type schema:PublicationVolume
124 N33fb45b1d1f94ae1a69f2c217c457c79 schema:name pubmed_id
125 schema:value 8994083
126 rdf:type schema:PropertyValue
127 N3a99b175c9d24412b35a1a64d07ccaee schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
128 schema:name Calcium
129 rdf:type schema:DefinedTerm
130 N3efc975b9a2c46b9ad37e534e6ebe851 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
131 schema:name Rotation
132 rdf:type schema:DefinedTerm
133 N4275151018534961b52566a831ec41b4 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
134 schema:name Magnesium
135 rdf:type schema:DefinedTerm
136 N44a70acb6aa94140a9db7ae51cb65420 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
137 schema:name Actins
138 rdf:type schema:DefinedTerm
139 N48be096fe38e43ccad8cdd923834a9a7 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
140 schema:name Animals
141 rdf:type schema:DefinedTerm
142 N533933361d6340a8a0e20bf9f3437447 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
143 schema:name Bivalvia
144 rdf:type schema:DefinedTerm
145 N57f8e26f00324c44bf5e04e3afe4284f schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
146 schema:name Glass
147 rdf:type schema:DefinedTerm
148 N739047ede9824833a740ad6075eec098 rdf:first sg:person.01160232013.39
149 rdf:rest N0bf578bb24454e0c9e2d6dd4d89c9d41
150 N762df787ef584e6d818b8e1ba206cb18 schema:affiliation grid-institutes:grid.136593.b
151 schema:familyName West
152 schema:givenName Jan M.
153 rdf:type schema:Person
154 N85b41c72d51d4cd3a59639f72f2d0bc6 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
155 schema:name Adenosine Triphosphate
156 rdf:type schema:DefinedTerm
157 N88a83f8c030c4000bbf443aca0ab1179 schema:issueNumber 6
158 rdf:type schema:PublicationIssue
159 Na7bf54b224e74dcd8dc9839c05ea7b16 rdf:first N762df787ef584e6d818b8e1ba206cb18
160 rdf:rest Nc8c3d5b80ee544858c5cf92a412b9c97
161 Nac9c5c5cf8ff44689f0ff24391aa3447 schema:name Springer Nature - SN SciGraph project
162 rdf:type schema:Organization
163 Nb9473148012d4f25bfb53ff33cb89c68 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
164 schema:name Rabbits
165 rdf:type schema:DefinedTerm
166 Nc8c3d5b80ee544858c5cf92a412b9c97 rdf:first sg:person.015604164731.98
167 rdf:rest N739047ede9824833a740ad6075eec098
168 Nc9fd049292654101988f43f1b0ad0035 schema:name dimensions_id
169 schema:value pub.1013437230
170 rdf:type schema:PropertyValue
171 Nd45ce61178424040a5708d1f08f2719c schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
172 schema:name Muscles
173 rdf:type schema:DefinedTerm
174 Nd5a89ca031314fb0adcf5fc0d7350f3e schema:name doi
175 schema:value 10.1007/bf00154058
176 rdf:type schema:PropertyValue
177 Neea95eb1210947ebbb427647fe0035c0 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
178 schema:name Myosins
179 rdf:type schema:DefinedTerm
180 Nff5e0e61b7f242d48af27219455975fc schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
181 schema:name Dose-Response Relationship, Drug
182 rdf:type schema:DefinedTerm
183 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
184 schema:name Biological Sciences
185 rdf:type schema:DefinedTerm
186 anzsrc-for:0601 schema:inDefinedTermSet anzsrc-for:
187 schema:name Biochemistry and Cell Biology
188 rdf:type schema:DefinedTerm
189 anzsrc-for:0606 schema:inDefinedTermSet anzsrc-for:
190 schema:name Physiology
191 rdf:type schema:DefinedTerm
192 sg:journal.1091334 schema:issn 0142-4319
193 1573-2657
194 schema:name Journal of Muscle Research and Cell Motility
195 schema:publisher Springer Nature
196 rdf:type schema:Periodical
197 sg:person.01160232013.39 schema:affiliation grid-institutes:grid.471052.5
198 schema:familyName Ishijima
199 schema:givenName Akihiko
200 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01160232013.39
201 rdf:type schema:Person
202 sg:person.015141357621.93 schema:affiliation grid-institutes:None
203 schema:familyName Yanagida
204 schema:givenName Toshio
205 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.015141357621.93
206 rdf:type schema:Person
207 sg:person.015604164731.98 schema:affiliation grid-institutes:None
208 schema:familyName Higuchi
209 schema:givenName Hideo
210 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.015604164731.98
211 rdf:type schema:Person
212 sg:pub.10.1038/289690a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1041755363
213 https://doi.org/10.1038/289690a0
214 rdf:type schema:CreativeWork
215 sg:pub.10.1038/339481a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1034823717
216 https://doi.org/10.1038/339481a0
217 rdf:type schema:CreativeWork
218 sg:pub.10.1038/341154a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1013597084
219 https://doi.org/10.1038/341154a0
220 rdf:type schema:CreativeWork
221 sg:pub.10.1038/368306a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1010508328
222 https://doi.org/10.1038/368306a0
223 rdf:type schema:CreativeWork
224 sg:pub.10.1038/nsb0494-209 schema:sameAs https://app.dimensions.ai/details/publication/pub.1014009654
225 https://doi.org/10.1038/nsb0494-209
226 rdf:type schema:CreativeWork
227 grid-institutes:None schema:alternateName Yanagida Biomotron Project, 2-4-14 Senba-Higashi, 562, Mino, Osaka, Japan
228 schema:name Department of Biophysical Engineering, Osaka University, Toyonaka, Osaka, Japan
229 Yanagida Biomotron Project, 2-4-14 Senba-Higashi, 562, Mino, Osaka, Japan
230 rdf:type schema:Organization
231 grid-institutes:grid.136593.b schema:alternateName Department of Biophysical Engineering, Osaka University, Toyonaka, Osaka, Japan
232 schema:name Department of Biophysical Engineering, Osaka University, Toyonaka, Osaka, Japan
233 rdf:type schema:Organization
234 grid-institutes:grid.471052.5 schema:alternateName Wako Research Centre, Honda R & D Co. Ltd, Wako, Saitama, Japan
235 schema:name Wako Research Centre, Honda R & D Co. Ltd, Wako, Saitama, Japan
236 Yanagida Biomotron Project, 2-4-14 Senba-Higashi, 562, Mino, Osaka, Japan
237 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...