Introduction of foreign DNA into Chlorella saccharophila by electroporation View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1994-11

AUTHORS

Masato Maruyama, Irena Horáková, Hiroyuki Honda, Xin-hui Xing, Naohiro Shiragami, Hajime Unno

ABSTRACT

Plasmid, pBI221, was introduced into protoplasts of Chlorella saccharophila c-211-1a prepared from the cells in the stationary phase by electroporation. Transient expression of the introduced plasmid was observed under a field strength of between 600 and 900 V/cm, and a pulse duration of around 400 ms, where high membrane permeability to 70-kDa FITC-dextran was ascertained. More... »

PAGES

821-826

Journal

TITLE

Biotechnology Letters

ISSUE

11

VOLUME

8

Author Affiliations

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  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/bf00152891

    DOI

    http://dx.doi.org/10.1007/bf00152891

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1009776912


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