Construction of integrated system for selection of fused plant protoplasts View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1993-10

AUTHORS

Kazuo Sakamoto, Kazuhiro Fujita, Kimiko Hirayae, Kumiko Iida, Takashi Koyano, Yoshihisa Asada, Tsutomu Furuya

ABSTRACT

An integrated system has been constructed to instantly identify and efficiently sort the heterokaryons formed by plant protoplast fusion. The system is composed of the following functions: a) a transport system, b) an electro-manipulator, c) a cell harvester, d) a flow cytometer/cell sorter, and e) a control device. The conditions for an efficient and reproducible enrichment of the heterokaryons have been investigated by this system using the fluorescein isothiocyanate stained protoplasts preparing from Glycyrrhiza glabra cell cultures and unstained protoplasts of Abrus precatorius cell cultures which contain a large quantity of chlorophyll. More... »

PAGES

749-754

Journal

TITLE

Biotechnology Letters

ISSUE

10

VOLUME

7

Author Affiliations

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf00152625

DOI

http://dx.doi.org/10.1007/bf00152625

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1024878891


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