Beaded matrices from cross-linked alginate for affinity and ion exchange chromatography of proteins View Full Text


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Article Info

DATE

1993-10

AUTHORS

M. Grasselli, L. E. Diaz, O. Cascone

ABSTRACT

To obtain a low cost, beaded chromatographic matrix, calcium alginate beads were cross-linked with epichlorohydrin, and calcium was removed by sodium citrate treatment. The cross-linking reaction for obtaining stable beads was optimized. For purification of haemoglobin by ion exchange, cross-linked calcium-free alginate beads have a qmax of 60 mg/ml and a Kd of 0.02 mg/ml gel, while for affinity polygalacturonase purification Kaff was 0.007 ml/g. More... »

PAGES

707-712

References to SciGraph publications

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf00152617

DOI

http://dx.doi.org/10.1007/bf00152617

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1031725975


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