Beaded matrices from cross-linked alginate for affinity and ion exchange chromatography of proteins View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1993-10

AUTHORS

M. Grasselli, L. E. Diaz, O. Cascone

ABSTRACT

To obtain a low cost, beaded chromatographic matrix, calcium alginate beads were cross-linked with epichlorohydrin, and calcium was removed by sodium citrate treatment. The cross-linking reaction for obtaining stable beads was optimized. For purification of haemoglobin by ion exchange, cross-linked calcium-free alginate beads have a qmax of 60 mg/ml and a Kd of 0.02 mg/ml gel, while for affinity polygalacturonase purification Kaff was 0.007 ml/g. More... »

PAGES

707-712

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf00152617

DOI

http://dx.doi.org/10.1007/bf00152617

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1031725975


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/1116", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Medical Physiology", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/11", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Medical and Health Sciences", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "University of Buenos Aires", 
          "id": "https://www.grid.ac/institutes/grid.7345.5", 
          "name": [
            "Facultad de Farmacia y Bioqu\u00edmica (UBA), C\u00e1tedras de Microbiolog\u00eda Industrial y Biotecnolog\u00eda y Qu\u00edmica Anal\u00edtica Instrumental, Jun\u00edn 956 - (1113), Buenos Aires, Argentina"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Grasselli", 
        "givenName": "M.", 
        "id": "sg:person.01301121133.13", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01301121133.13"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Buenos Aires", 
          "id": "https://www.grid.ac/institutes/grid.7345.5", 
          "name": [
            "Facultad de Farmacia y Bioqu\u00edmica (UBA), C\u00e1tedras de Microbiolog\u00eda Industrial y Biotecnolog\u00eda y Qu\u00edmica Anal\u00edtica Instrumental, Jun\u00edn 956 - (1113), Buenos Aires, Argentina"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Diaz", 
        "givenName": "L. E.", 
        "id": "sg:person.01245203272.53", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01245203272.53"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "University of Buenos Aires", 
          "id": "https://www.grid.ac/institutes/grid.7345.5", 
          "name": [
            "Facultad de Farmacia y Bioqu\u00edmica (UBA), C\u00e1tedras de Microbiolog\u00eda Industrial y Biotecnolog\u00eda y Qu\u00edmica Anal\u00edtica Instrumental, Jun\u00edn 956 - (1113), Buenos Aires, Argentina"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Cascone", 
        "givenName": "O.", 
        "id": "sg:person.01062160206.02", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01062160206.02"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.1002/bit.260270718", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1007031613"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/btpr.5420030407", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1014332823"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0005-2744(72)90213-6", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1016571923"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0005-2744(72)90213-6", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1016571923"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/978-94-009-0783-6_17", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1022632226", 
          "https://doi.org/10.1007/978-94-009-0783-6_17"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0014-5793(73)80770-7", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1022803134"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0300-9467(89)80046-2", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1023421960"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/978-94-009-0783-6_35", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1031543870", 
          "https://doi.org/10.1007/978-94-009-0783-6_35"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0268-005x(09)80296-8", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1035039844"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0021-9673(00)80704-9", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1042167418"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/bit.260270617", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1051913877"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1082426041", 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "1993-10", 
    "datePublishedReg": "1993-10-01", 
    "description": "To obtain a low cost, beaded chromatographic matrix, calcium alginate beads were cross-linked with epichlorohydrin, and calcium was removed by sodium citrate treatment. The cross-linking reaction for obtaining stable beads was optimized. For purification of haemoglobin by ion exchange, cross-linked calcium-free alginate beads have a qmax of 60 mg/ml and a Kd of 0.02 mg/ml gel, while for affinity polygalacturonase purification Kaff was 0.007 ml/g.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1007/bf00152617", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": false, 
    "isPartOf": [
      {
        "id": "sg:journal.1289037", 
        "issn": [
          "0951-208X", 
          "1573-6784"
        ], 
        "name": "Biotechnology Letters", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "10", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "7"
      }
    ], 
    "name": "Beaded matrices from cross-linked alginate for affinity and ion exchange chromatography of proteins", 
    "pagination": "707-712", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "9722ae0640675e64911a52585f525502dc2a9fb26e713af48457035acc0d124e"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1007/bf00152617"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1031725975"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1007/bf00152617", 
      "https://app.dimensions.ai/details/publication/pub.1031725975"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-11T13:49", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000371_0000000371/records_130794_00000002.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "http://link.springer.com/10.1007/BF00152617"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/bf00152617'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/bf00152617'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/bf00152617'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/bf00152617'


 

This table displays all metadata directly associated to this object as RDF triples.

109 TRIPLES      21 PREDICATES      38 URIs      19 LITERALS      7 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1007/bf00152617 schema:about anzsrc-for:11
2 anzsrc-for:1116
3 schema:author N89b9392d5f1243078b93a52479e9eb8f
4 schema:citation sg:pub.10.1007/978-94-009-0783-6_17
5 sg:pub.10.1007/978-94-009-0783-6_35
6 https://app.dimensions.ai/details/publication/pub.1082426041
7 https://doi.org/10.1002/bit.260270617
8 https://doi.org/10.1002/bit.260270718
9 https://doi.org/10.1002/btpr.5420030407
10 https://doi.org/10.1016/0005-2744(72)90213-6
11 https://doi.org/10.1016/0014-5793(73)80770-7
12 https://doi.org/10.1016/0300-9467(89)80046-2
13 https://doi.org/10.1016/s0021-9673(00)80704-9
14 https://doi.org/10.1016/s0268-005x(09)80296-8
15 schema:datePublished 1993-10
16 schema:datePublishedReg 1993-10-01
17 schema:description To obtain a low cost, beaded chromatographic matrix, calcium alginate beads were cross-linked with epichlorohydrin, and calcium was removed by sodium citrate treatment. The cross-linking reaction for obtaining stable beads was optimized. For purification of haemoglobin by ion exchange, cross-linked calcium-free alginate beads have a qmax of 60 mg/ml and a Kd of 0.02 mg/ml gel, while for affinity polygalacturonase purification Kaff was 0.007 ml/g.
18 schema:genre research_article
19 schema:inLanguage en
20 schema:isAccessibleForFree false
21 schema:isPartOf N36201d7eb3534e81bfea05173219c539
22 Nc4253a8cef6a44ba9fcf48d02c46cdde
23 sg:journal.1289037
24 schema:name Beaded matrices from cross-linked alginate for affinity and ion exchange chromatography of proteins
25 schema:pagination 707-712
26 schema:productId N10faa9c1c6424d038f354485423e63a6
27 N13ff8ae8355a42b3a0866498060d4f26
28 N3d5e8240e9da4e77aed981a313df2362
29 schema:sameAs https://app.dimensions.ai/details/publication/pub.1031725975
30 https://doi.org/10.1007/bf00152617
31 schema:sdDatePublished 2019-04-11T13:49
32 schema:sdLicense https://scigraph.springernature.com/explorer/license/
33 schema:sdPublisher Nbb016bf40e7d411095200fef728d512c
34 schema:url http://link.springer.com/10.1007/BF00152617
35 sgo:license sg:explorer/license/
36 sgo:sdDataset articles
37 rdf:type schema:ScholarlyArticle
38 N10faa9c1c6424d038f354485423e63a6 schema:name doi
39 schema:value 10.1007/bf00152617
40 rdf:type schema:PropertyValue
41 N13ff8ae8355a42b3a0866498060d4f26 schema:name dimensions_id
42 schema:value pub.1031725975
43 rdf:type schema:PropertyValue
44 N36201d7eb3534e81bfea05173219c539 schema:volumeNumber 7
45 rdf:type schema:PublicationVolume
46 N3d5e8240e9da4e77aed981a313df2362 schema:name readcube_id
47 schema:value 9722ae0640675e64911a52585f525502dc2a9fb26e713af48457035acc0d124e
48 rdf:type schema:PropertyValue
49 N5ca56bc71efc4988b5fe75566204dfbc rdf:first sg:person.01062160206.02
50 rdf:rest rdf:nil
51 N89b9392d5f1243078b93a52479e9eb8f rdf:first sg:person.01301121133.13
52 rdf:rest Ndecee261d77d47f2bdb9c29d2e761061
53 Nbb016bf40e7d411095200fef728d512c schema:name Springer Nature - SN SciGraph project
54 rdf:type schema:Organization
55 Nc4253a8cef6a44ba9fcf48d02c46cdde schema:issueNumber 10
56 rdf:type schema:PublicationIssue
57 Ndecee261d77d47f2bdb9c29d2e761061 rdf:first sg:person.01245203272.53
58 rdf:rest N5ca56bc71efc4988b5fe75566204dfbc
59 anzsrc-for:11 schema:inDefinedTermSet anzsrc-for:
60 schema:name Medical and Health Sciences
61 rdf:type schema:DefinedTerm
62 anzsrc-for:1116 schema:inDefinedTermSet anzsrc-for:
63 schema:name Medical Physiology
64 rdf:type schema:DefinedTerm
65 sg:journal.1289037 schema:issn 0951-208X
66 1573-6784
67 schema:name Biotechnology Letters
68 rdf:type schema:Periodical
69 sg:person.01062160206.02 schema:affiliation https://www.grid.ac/institutes/grid.7345.5
70 schema:familyName Cascone
71 schema:givenName O.
72 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01062160206.02
73 rdf:type schema:Person
74 sg:person.01245203272.53 schema:affiliation https://www.grid.ac/institutes/grid.7345.5
75 schema:familyName Diaz
76 schema:givenName L. E.
77 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01245203272.53
78 rdf:type schema:Person
79 sg:person.01301121133.13 schema:affiliation https://www.grid.ac/institutes/grid.7345.5
80 schema:familyName Grasselli
81 schema:givenName M.
82 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01301121133.13
83 rdf:type schema:Person
84 sg:pub.10.1007/978-94-009-0783-6_17 schema:sameAs https://app.dimensions.ai/details/publication/pub.1022632226
85 https://doi.org/10.1007/978-94-009-0783-6_17
86 rdf:type schema:CreativeWork
87 sg:pub.10.1007/978-94-009-0783-6_35 schema:sameAs https://app.dimensions.ai/details/publication/pub.1031543870
88 https://doi.org/10.1007/978-94-009-0783-6_35
89 rdf:type schema:CreativeWork
90 https://app.dimensions.ai/details/publication/pub.1082426041 schema:CreativeWork
91 https://doi.org/10.1002/bit.260270617 schema:sameAs https://app.dimensions.ai/details/publication/pub.1051913877
92 rdf:type schema:CreativeWork
93 https://doi.org/10.1002/bit.260270718 schema:sameAs https://app.dimensions.ai/details/publication/pub.1007031613
94 rdf:type schema:CreativeWork
95 https://doi.org/10.1002/btpr.5420030407 schema:sameAs https://app.dimensions.ai/details/publication/pub.1014332823
96 rdf:type schema:CreativeWork
97 https://doi.org/10.1016/0005-2744(72)90213-6 schema:sameAs https://app.dimensions.ai/details/publication/pub.1016571923
98 rdf:type schema:CreativeWork
99 https://doi.org/10.1016/0014-5793(73)80770-7 schema:sameAs https://app.dimensions.ai/details/publication/pub.1022803134
100 rdf:type schema:CreativeWork
101 https://doi.org/10.1016/0300-9467(89)80046-2 schema:sameAs https://app.dimensions.ai/details/publication/pub.1023421960
102 rdf:type schema:CreativeWork
103 https://doi.org/10.1016/s0021-9673(00)80704-9 schema:sameAs https://app.dimensions.ai/details/publication/pub.1042167418
104 rdf:type schema:CreativeWork
105 https://doi.org/10.1016/s0268-005x(09)80296-8 schema:sameAs https://app.dimensions.ai/details/publication/pub.1035039844
106 rdf:type schema:CreativeWork
107 https://www.grid.ac/institutes/grid.7345.5 schema:alternateName University of Buenos Aires
108 schema:name Facultad de Farmacia y Bioquímica (UBA), Cátedras de Microbiología Industrial y Biotecnología y Química Analítica Instrumental, Junín 956 - (1113), Buenos Aires, Argentina
109 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...