Detection of the metastatic potential of blood-borne and immunomagnetically enriched epithelial cells by quantitative erbB-2 RT-PCR View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1996-09

AUTHORS

B. Brandt, C. Griwatz, S. Heidl, G. Assmann, K. S. Zänker

ABSTRACT

A quantitative competitive RT-PCR targeting the specific mRNA of c-erbB-2 is described. It is well known that in many cancers the c-erbB-2 gene dosage is elevated and/or the mRNA is overexpressed leading to a poor prognosis. The recently established method to enrich epithelial cells from peripheral blood made it reasonable and logical to develop a sophisticated competitive RT-PCR method to estimate the c-erbB-2 mRNA load of such cells. By this enrichment method it was discovered that cancer cells can be either isolated as single cells or clusters from patients' blood. The plausibility for their metastatic potential is derived from the measurement of the c-erbB-2 mRNA expression. A SKBR3 cell model suggested that the c-erbB-2 mRNA load is between 168 and 336 molecules per cell. Results of the quantitative competitive RT-PCR method can be used as a rational to determine the efficiency of anti-oncogenic therapies and as a criterion in adjuvant therapy decision. More... »

PAGES

399-408

References to SciGraph publications

Journal

TITLE

Clinical & Experimental Metastasis

ISSUE

4

VOLUME

14

Related Patents

  • Modified Polynucleotides For The Production Of Secreted Proteins
  • Engineered Nucleic Acids Encoding A Modified Erythropoietin And Their Expression
  • Application Of Mrna For Use As A Therapeutic Against Tumor Diseases
  • Application Of Mrna For Use As A Therapeutic Agent For Tumorous Diseases
  • Modified Polynucleotides For The Production Of Biologics And Proteins Associated With Human Disease
  • Modified Polynucleotides For Treating Galactosylceramidase Protein Deficiency
  • Modified Polynucleotides Encoding Granulysin
  • Modified Polynucleotides Encoding Apoptosis Inducing Factor 1
  • Modified Polynucleotides For The Production Of Nuclear Proteins
  • Modified Polynucleotides Encoding Cited4
  • Modified Polynucleotides Encoding Septin-4
  • Engineered Nucleic Acids And Methods Of Use Thereof
  • Application Of Mrna For Use As A Therapeutic Against Tumour Diseases
  • Modified Nucleosides, Nucleotides, And Nucleic Acids, And Uses Thereof
  • Application Of Mrna For Use As A Therapeutic Against Tumour Diseases
  • Modified Polynucleotides For Treating Protein Deficiency
  • Delivery And Formulation Of Engineered Nucleic Acids
  • Modified Polynucleotides For The Production Of Proteins Associated With Human Disease
  • Delivery And Formulation Of Engineered Nucleic Acids
  • Engineered Nucleic Acids And Methods Of Use Thereof
  • Modified Polynucleotides Encoding Copper Metabolism (Murr1) Domain Containing 1
  • Engineered Nucleic Acids And Methods Of Use Thereof
  • Formulation And Delivery Of Plga Microspheres
  • Application Of Mrna For Use As A Therapeutic Against Tumour Diseases
  • Compositions And Methods Of Altering Cholesterol Levels
  • Modified Polynucleotides Encoding Basic Helix-Loop-Helix Family Member E41
  • Modified Polynucleotides For The Production Of Proteins
  • Engineered Nucleic Acids And Methods Of Use Thereof
  • Engineered Nucleic Acids And Methods Of Use Thereof
  • Modified Polynucleotides For The Production Of Oncology-Related Proteins And Peptides
  • Engineered Nucleic Acids And Methods Of Use Thereof
  • Modified Polynucleotides For The Production Of G-Csf
  • Modified Nucleosides, Nucleotides, And Nucleic Acids, And Uses Thereof
  • Engineered Nucleic Acids And Methods Of Use Thereof
  • Terminally Modified Rna
  • Modified Polynucleotides For The Production Of Cosmetic Proteins And Peptides
  • Application Of Mrna For Use As A Therapeutic Against Tumour Diseases
  • In Vivo Production Of Proteins
  • Application Of Mrna For Use As A Therapeutic Against Tumour Diseases
  • Modified Polynucleotides Encoding Siah E3 Ubiquitin Protein Ligase 1
  • Modified Polynucleotides Encoding Hepatitis A Virus Cellular Receptor 2
  • Dlin-Kc2-Dma Lipid Nanoparticle Delivery Of Modified Polynucleotides
  • Modified Polynucleotides For The Production Of Secreted Proteins
  • Modified Polynucleotides For The Production Of Proteins
  • Method For Producing A Protein Of Interest In A Primate
  • Modified Nucleosides, Nucleotides, And Nucleic Acids, And Uses Thereof
  • Modified Polynucleotides For The Production Of Cytoplasmic And Cytoskeletal Proteins
  • Modified Polynucleotides For The Production Of Cytoplasmic And Cytoskeletal Proteins
  • Modified Polynucleotides Encoding Aquaporin-5
  • Modified Polynucleotides For The Production Of Proteins Associated With Blood And Lymphatic Disorders
  • Modified Polynucleotides Encoding Aryl Hydrocarbon Receptor Nuclear Translocator
  • Modified Polynucleotides For The Production Of Proteins Associated With Blood And Lymphatic Disorders
  • Modified Nucleosides, Nucleotides, And Nucleic Acids, And Uses Thereof
  • Split Dose Administration
  • Modified Polynucleotides For The Production Of Biologics And Proteins Associated With Human Disease
  • Formulation And Delivery Of Plga Microspheres
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/bf00123399

    DOI

    http://dx.doi.org/10.1007/bf00123399

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1044609615

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/8878414


    Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
    Incoming Citations Browse incoming citations for this publication using opencitations.net

    JSON-LD is the canonical representation for SciGraph data.

    TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

    [
      {
        "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
        "about": [
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/1112", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Oncology and Carcinogenesis", 
            "type": "DefinedTerm"
          }, 
          {
            "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/11", 
            "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
            "name": "Medical and Health Sciences", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Adenocarcinoma", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Breast Neoplasms", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Epithelial Cells", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Epithelium", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Gene Expression Regulation, Neoplastic", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Genes, erbB-2", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Humans", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Magnetics", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Neoplastic Cells, Circulating", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Oncogene Proteins v-erbB", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Polymerase Chain Reaction", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "RNA, Messenger", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Transcription, Genetic", 
            "type": "DefinedTerm"
          }, 
          {
            "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
            "name": "Tumor Cells, Cultured", 
            "type": "DefinedTerm"
          }
        ], 
        "author": [
          {
            "affiliation": {
              "alternateName": "University of M\u00fcnster", 
              "id": "https://www.grid.ac/institutes/grid.5949.1", 
              "name": [
                "Institute of Clinical Chemistry and Laboratory Medicine, Westf\u00e4lische Wilhelms-Universit\u00e4t M\u00fcnster, M\u00fcnster, Germany"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Brandt", 
            "givenName": "B.", 
            "id": "sg:person.0763712472.31", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0763712472.31"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Witten/Herdecke University", 
              "id": "https://www.grid.ac/institutes/grid.412581.b", 
              "name": [
                "Institute of Immunology, University Witten/Herdecke, Witten, Germany"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Griwatz", 
            "givenName": "C.", 
            "id": "sg:person.0606067135.30", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0606067135.30"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "University of M\u00fcnster", 
              "id": "https://www.grid.ac/institutes/grid.5949.1", 
              "name": [
                "Institute of Clinical Chemistry and Laboratory Medicine, Westf\u00e4lische Wilhelms-Universit\u00e4t M\u00fcnster, M\u00fcnster, Germany"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Heidl", 
            "givenName": "S.", 
            "id": "sg:person.0654202335.27", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0654202335.27"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "University of M\u00fcnster", 
              "id": "https://www.grid.ac/institutes/grid.5949.1", 
              "name": [
                "Institute of Clinical Chemistry and Laboratory Medicine, Westf\u00e4lische Wilhelms-Universit\u00e4t M\u00fcnster, M\u00fcnster, Germany"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Assmann", 
            "givenName": "G.", 
            "id": "sg:person.0662640572.84", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0662640572.84"
            ], 
            "type": "Person"
          }, 
          {
            "affiliation": {
              "alternateName": "Witten/Herdecke University", 
              "id": "https://www.grid.ac/institutes/grid.412581.b", 
              "name": [
                "Institute of Immunology, University Witten/Herdecke, Witten, Germany"
              ], 
              "type": "Organization"
            }, 
            "familyName": "Z\u00e4nker", 
            "givenName": "K. S.", 
            "id": "sg:person.01315037564.16", 
            "sameAs": [
              "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01315037564.16"
            ], 
            "type": "Person"
          }
        ], 
        "citation": [
          {
            "id": "https://doi.org/10.1097/00019606-199309000-00009", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1005247991"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1097/00019606-199309000-00009", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1005247991"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1073/pnas.82.15.4920", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1008941231"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0277-5379(91)90032-9", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1009036155"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0092-8674(82)90400-7", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1012378105"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0022-1759(95)00063-g", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1013451976"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0167-4781(94)90076-0", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1016402246"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0167-4781(94)90076-0", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1016402246"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/bjc.1995.56", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1019066996", 
              "https://doi.org/10.1038/bjc.1995.56"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/bjc.1995.56", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1019066996", 
              "https://doi.org/10.1038/bjc.1995.56"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0378-1119(95)00147-x", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1019363042"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0378-1119(94)00522-t", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1022514831"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/bjc.1991.375", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1023329524", 
              "https://doi.org/10.1038/bjc.1991.375"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "sg:pub.10.1038/bjc.1991.375", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1023329524", 
              "https://doi.org/10.1038/bjc.1991.375"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1083/jcb.111.2.567", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1033671488"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0378-1119(94)00651-8", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1037411313"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1073/pnas.82.19.6497", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1037809345"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1016/0378-1119(94)00786-r", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1050674574"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1093/jnci/85.11.897", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1059817957"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1093/jnci/85.17.1419", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1059818126"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1097/00019606-199303000-00029", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1060293920"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1126/science.2470152", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1062539373"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://app.dimensions.ai/details/publication/pub.1077297637", 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://app.dimensions.ai/details/publication/pub.1077365384", 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1200/jco.1994.12.3.475", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1082392048"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://doi.org/10.1200/jco.1994.12.4.725", 
            "sameAs": [
              "https://app.dimensions.ai/details/publication/pub.1082392959"
            ], 
            "type": "CreativeWork"
          }, 
          {
            "id": "https://app.dimensions.ai/details/publication/pub.1082410505", 
            "type": "CreativeWork"
          }
        ], 
        "datePublished": "1996-09", 
        "datePublishedReg": "1996-09-01", 
        "description": "A quantitative competitive RT-PCR targeting the specific mRNA of c-erbB-2 is described. It is well known that in many cancers the c-erbB-2 gene dosage is elevated and/or the mRNA is overexpressed leading to a poor prognosis. The recently established method to enrich epithelial cells from peripheral blood made it reasonable and logical to develop a sophisticated competitive RT-PCR method to estimate the c-erbB-2 mRNA load of such cells. By this enrichment method it was discovered that cancer cells can be either isolated as single cells or clusters from patients' blood. The plausibility for their metastatic potential is derived from the measurement of the c-erbB-2 mRNA expression. A SKBR3 cell model suggested that the c-erbB-2 mRNA load is between 168 and 336 molecules per cell. Results of the quantitative competitive RT-PCR method can be used as a rational to determine the efficiency of anti-oncogenic therapies and as a criterion in adjuvant therapy decision.", 
        "genre": "research_article", 
        "id": "sg:pub.10.1007/bf00123399", 
        "inLanguage": [
          "en"
        ], 
        "isAccessibleForFree": false, 
        "isPartOf": [
          {
            "id": "sg:journal.1094856", 
            "issn": [
              "0262-0898", 
              "1573-7276"
            ], 
            "name": "Clinical & Experimental Metastasis", 
            "type": "Periodical"
          }, 
          {
            "issueNumber": "4", 
            "type": "PublicationIssue"
          }, 
          {
            "type": "PublicationVolume", 
            "volumeNumber": "14"
          }
        ], 
        "name": "Detection of the metastatic potential of blood-borne and immunomagnetically enriched epithelial cells by quantitative erbB-2 RT-PCR", 
        "pagination": "399-408", 
        "productId": [
          {
            "name": "readcube_id", 
            "type": "PropertyValue", 
            "value": [
              "d47e0a2899af1af10ffc7fdc4657fb7a0c64a578094594442a49e88902e14d3c"
            ]
          }, 
          {
            "name": "pubmed_id", 
            "type": "PropertyValue", 
            "value": [
              "8878414"
            ]
          }, 
          {
            "name": "nlm_unique_id", 
            "type": "PropertyValue", 
            "value": [
              "8409970"
            ]
          }, 
          {
            "name": "doi", 
            "type": "PropertyValue", 
            "value": [
              "10.1007/bf00123399"
            ]
          }, 
          {
            "name": "dimensions_id", 
            "type": "PropertyValue", 
            "value": [
              "pub.1044609615"
            ]
          }
        ], 
        "sameAs": [
          "https://doi.org/10.1007/bf00123399", 
          "https://app.dimensions.ai/details/publication/pub.1044609615"
        ], 
        "sdDataset": "articles", 
        "sdDatePublished": "2019-04-11T13:48", 
        "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
        "sdPublisher": {
          "name": "Springer Nature - SN SciGraph project", 
          "type": "Organization"
        }, 
        "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000371_0000000371/records_130793_00000004.jsonl", 
        "type": "ScholarlyArticle", 
        "url": "http://link.springer.com/10.1007/BF00123399"
      }
    ]
     

    Download the RDF metadata as:  json-ld nt turtle xml License info

    HOW TO GET THIS DATA PROGRAMMATICALLY:

    JSON-LD is a popular format for linked data which is fully compatible with JSON.

    curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/bf00123399'

    N-Triples is a line-based linked data format ideal for batch operations.

    curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/bf00123399'

    Turtle is a human-readable linked data format.

    curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/bf00123399'

    RDF/XML is a standard XML format for linked data.

    curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/bf00123399'


     

    This table displays all metadata directly associated to this object as RDF triples.

    224 TRIPLES      21 PREDICATES      66 URIs      35 LITERALS      23 BLANK NODES

    Subject Predicate Object
    1 sg:pub.10.1007/bf00123399 schema:about N0b87b2b9de084cd29de94ccef801fd5b
    2 N0bb91f6db13340278228ae9f94c81a5f
    3 N4944dccdd90a45c08d8480d7bcb1d4a7
    4 N5cf513827a3c4d4a9f9cc080a57c6e48
    5 N5e3595caf4c142c09f25adc20155d881
    6 N63d11597851242b09d0bff75dc2855a7
    7 N64c7f42237c842f1b32e554fc3a790a9
    8 N7a1741ae5e8d48dc93365de798b04916
    9 N95e7fa10d547493eb617aa29cd1c81ad
    10 N997e40cc02a344a79606c77a38118229
    11 Na9321e7475dd4790b9dbe9cf58c1eb17
    12 Nb8ba11c89b324839b749dcdd56f5baa1
    13 Ncdd1216dc49c427a94b349402f718487
    14 Nf5b1e3f17883468eac87d1e4922ce7e6
    15 anzsrc-for:11
    16 anzsrc-for:1112
    17 schema:author N0fbad19841564d02b3f0f0ba0abe3508
    18 schema:citation sg:pub.10.1038/bjc.1991.375
    19 sg:pub.10.1038/bjc.1995.56
    20 https://app.dimensions.ai/details/publication/pub.1077297637
    21 https://app.dimensions.ai/details/publication/pub.1077365384
    22 https://app.dimensions.ai/details/publication/pub.1082410505
    23 https://doi.org/10.1016/0022-1759(95)00063-g
    24 https://doi.org/10.1016/0092-8674(82)90400-7
    25 https://doi.org/10.1016/0167-4781(94)90076-0
    26 https://doi.org/10.1016/0277-5379(91)90032-9
    27 https://doi.org/10.1016/0378-1119(94)00522-t
    28 https://doi.org/10.1016/0378-1119(94)00651-8
    29 https://doi.org/10.1016/0378-1119(94)00786-r
    30 https://doi.org/10.1016/0378-1119(95)00147-x
    31 https://doi.org/10.1073/pnas.82.15.4920
    32 https://doi.org/10.1073/pnas.82.19.6497
    33 https://doi.org/10.1083/jcb.111.2.567
    34 https://doi.org/10.1093/jnci/85.11.897
    35 https://doi.org/10.1093/jnci/85.17.1419
    36 https://doi.org/10.1097/00019606-199303000-00029
    37 https://doi.org/10.1097/00019606-199309000-00009
    38 https://doi.org/10.1126/science.2470152
    39 https://doi.org/10.1200/jco.1994.12.3.475
    40 https://doi.org/10.1200/jco.1994.12.4.725
    41 schema:datePublished 1996-09
    42 schema:datePublishedReg 1996-09-01
    43 schema:description A quantitative competitive RT-PCR targeting the specific mRNA of c-erbB-2 is described. It is well known that in many cancers the c-erbB-2 gene dosage is elevated and/or the mRNA is overexpressed leading to a poor prognosis. The recently established method to enrich epithelial cells from peripheral blood made it reasonable and logical to develop a sophisticated competitive RT-PCR method to estimate the c-erbB-2 mRNA load of such cells. By this enrichment method it was discovered that cancer cells can be either isolated as single cells or clusters from patients' blood. The plausibility for their metastatic potential is derived from the measurement of the c-erbB-2 mRNA expression. A SKBR3 cell model suggested that the c-erbB-2 mRNA load is between 168 and 336 molecules per cell. Results of the quantitative competitive RT-PCR method can be used as a rational to determine the efficiency of anti-oncogenic therapies and as a criterion in adjuvant therapy decision.
    44 schema:genre research_article
    45 schema:inLanguage en
    46 schema:isAccessibleForFree false
    47 schema:isPartOf N2875b97576744e5e9a63e11b7efdaabf
    48 Ne0d04cf0c3c14e6cba108564be991e70
    49 sg:journal.1094856
    50 schema:name Detection of the metastatic potential of blood-borne and immunomagnetically enriched epithelial cells by quantitative erbB-2 RT-PCR
    51 schema:pagination 399-408
    52 schema:productId N191ae0d3fabe4cb2af4609ff6203c706
    53 N2c774057dd4940518d0f71579638d394
    54 N75a75459dac846f992b3e164dda218a4
    55 N8710d78a36e948dd945042294ed8f546
    56 Nebacbb21e4b64248a2832fc4064dff1e
    57 schema:sameAs https://app.dimensions.ai/details/publication/pub.1044609615
    58 https://doi.org/10.1007/bf00123399
    59 schema:sdDatePublished 2019-04-11T13:48
    60 schema:sdLicense https://scigraph.springernature.com/explorer/license/
    61 schema:sdPublisher Ne3f5adf55c784ed5802c8a87ec12eb5b
    62 schema:url http://link.springer.com/10.1007/BF00123399
    63 sgo:license sg:explorer/license/
    64 sgo:sdDataset articles
    65 rdf:type schema:ScholarlyArticle
    66 N0b87b2b9de084cd29de94ccef801fd5b schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    67 schema:name Magnetics
    68 rdf:type schema:DefinedTerm
    69 N0bb91f6db13340278228ae9f94c81a5f schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    70 schema:name RNA, Messenger
    71 rdf:type schema:DefinedTerm
    72 N0fbad19841564d02b3f0f0ba0abe3508 rdf:first sg:person.0763712472.31
    73 rdf:rest N2d9b1533238049cc894dc5802a6f847c
    74 N10d5ec5aa33c46a58066a4c952a813f3 rdf:first sg:person.01315037564.16
    75 rdf:rest rdf:nil
    76 N191ae0d3fabe4cb2af4609ff6203c706 schema:name pubmed_id
    77 schema:value 8878414
    78 rdf:type schema:PropertyValue
    79 N2875b97576744e5e9a63e11b7efdaabf schema:volumeNumber 14
    80 rdf:type schema:PublicationVolume
    81 N2c774057dd4940518d0f71579638d394 schema:name readcube_id
    82 schema:value d47e0a2899af1af10ffc7fdc4657fb7a0c64a578094594442a49e88902e14d3c
    83 rdf:type schema:PropertyValue
    84 N2d9b1533238049cc894dc5802a6f847c rdf:first sg:person.0606067135.30
    85 rdf:rest Naccc2f487a024410bed326f9ad863605
    86 N4944dccdd90a45c08d8480d7bcb1d4a7 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    87 schema:name Epithelial Cells
    88 rdf:type schema:DefinedTerm
    89 N5cf513827a3c4d4a9f9cc080a57c6e48 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    90 schema:name Polymerase Chain Reaction
    91 rdf:type schema:DefinedTerm
    92 N5e3595caf4c142c09f25adc20155d881 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    93 schema:name Oncogene Proteins v-erbB
    94 rdf:type schema:DefinedTerm
    95 N63d11597851242b09d0bff75dc2855a7 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    96 schema:name Humans
    97 rdf:type schema:DefinedTerm
    98 N64c7f42237c842f1b32e554fc3a790a9 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    99 schema:name Breast Neoplasms
    100 rdf:type schema:DefinedTerm
    101 N75a75459dac846f992b3e164dda218a4 schema:name dimensions_id
    102 schema:value pub.1044609615
    103 rdf:type schema:PropertyValue
    104 N7a1741ae5e8d48dc93365de798b04916 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    105 schema:name Adenocarcinoma
    106 rdf:type schema:DefinedTerm
    107 N8710d78a36e948dd945042294ed8f546 schema:name nlm_unique_id
    108 schema:value 8409970
    109 rdf:type schema:PropertyValue
    110 N95e7fa10d547493eb617aa29cd1c81ad schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    111 schema:name Epithelium
    112 rdf:type schema:DefinedTerm
    113 N997e40cc02a344a79606c77a38118229 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    114 schema:name Transcription, Genetic
    115 rdf:type schema:DefinedTerm
    116 Na58eec28218a4e00a89758684a54ec0d rdf:first sg:person.0662640572.84
    117 rdf:rest N10d5ec5aa33c46a58066a4c952a813f3
    118 Na9321e7475dd4790b9dbe9cf58c1eb17 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    119 schema:name Tumor Cells, Cultured
    120 rdf:type schema:DefinedTerm
    121 Naccc2f487a024410bed326f9ad863605 rdf:first sg:person.0654202335.27
    122 rdf:rest Na58eec28218a4e00a89758684a54ec0d
    123 Nb8ba11c89b324839b749dcdd56f5baa1 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    124 schema:name Neoplastic Cells, Circulating
    125 rdf:type schema:DefinedTerm
    126 Ncdd1216dc49c427a94b349402f718487 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    127 schema:name Genes, erbB-2
    128 rdf:type schema:DefinedTerm
    129 Ne0d04cf0c3c14e6cba108564be991e70 schema:issueNumber 4
    130 rdf:type schema:PublicationIssue
    131 Ne3f5adf55c784ed5802c8a87ec12eb5b schema:name Springer Nature - SN SciGraph project
    132 rdf:type schema:Organization
    133 Nebacbb21e4b64248a2832fc4064dff1e schema:name doi
    134 schema:value 10.1007/bf00123399
    135 rdf:type schema:PropertyValue
    136 Nf5b1e3f17883468eac87d1e4922ce7e6 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
    137 schema:name Gene Expression Regulation, Neoplastic
    138 rdf:type schema:DefinedTerm
    139 anzsrc-for:11 schema:inDefinedTermSet anzsrc-for:
    140 schema:name Medical and Health Sciences
    141 rdf:type schema:DefinedTerm
    142 anzsrc-for:1112 schema:inDefinedTermSet anzsrc-for:
    143 schema:name Oncology and Carcinogenesis
    144 rdf:type schema:DefinedTerm
    145 sg:journal.1094856 schema:issn 0262-0898
    146 1573-7276
    147 schema:name Clinical & Experimental Metastasis
    148 rdf:type schema:Periodical
    149 sg:person.01315037564.16 schema:affiliation https://www.grid.ac/institutes/grid.412581.b
    150 schema:familyName Zänker
    151 schema:givenName K. S.
    152 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01315037564.16
    153 rdf:type schema:Person
    154 sg:person.0606067135.30 schema:affiliation https://www.grid.ac/institutes/grid.412581.b
    155 schema:familyName Griwatz
    156 schema:givenName C.
    157 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0606067135.30
    158 rdf:type schema:Person
    159 sg:person.0654202335.27 schema:affiliation https://www.grid.ac/institutes/grid.5949.1
    160 schema:familyName Heidl
    161 schema:givenName S.
    162 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0654202335.27
    163 rdf:type schema:Person
    164 sg:person.0662640572.84 schema:affiliation https://www.grid.ac/institutes/grid.5949.1
    165 schema:familyName Assmann
    166 schema:givenName G.
    167 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0662640572.84
    168 rdf:type schema:Person
    169 sg:person.0763712472.31 schema:affiliation https://www.grid.ac/institutes/grid.5949.1
    170 schema:familyName Brandt
    171 schema:givenName B.
    172 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0763712472.31
    173 rdf:type schema:Person
    174 sg:pub.10.1038/bjc.1991.375 schema:sameAs https://app.dimensions.ai/details/publication/pub.1023329524
    175 https://doi.org/10.1038/bjc.1991.375
    176 rdf:type schema:CreativeWork
    177 sg:pub.10.1038/bjc.1995.56 schema:sameAs https://app.dimensions.ai/details/publication/pub.1019066996
    178 https://doi.org/10.1038/bjc.1995.56
    179 rdf:type schema:CreativeWork
    180 https://app.dimensions.ai/details/publication/pub.1077297637 schema:CreativeWork
    181 https://app.dimensions.ai/details/publication/pub.1077365384 schema:CreativeWork
    182 https://app.dimensions.ai/details/publication/pub.1082410505 schema:CreativeWork
    183 https://doi.org/10.1016/0022-1759(95)00063-g schema:sameAs https://app.dimensions.ai/details/publication/pub.1013451976
    184 rdf:type schema:CreativeWork
    185 https://doi.org/10.1016/0092-8674(82)90400-7 schema:sameAs https://app.dimensions.ai/details/publication/pub.1012378105
    186 rdf:type schema:CreativeWork
    187 https://doi.org/10.1016/0167-4781(94)90076-0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1016402246
    188 rdf:type schema:CreativeWork
    189 https://doi.org/10.1016/0277-5379(91)90032-9 schema:sameAs https://app.dimensions.ai/details/publication/pub.1009036155
    190 rdf:type schema:CreativeWork
    191 https://doi.org/10.1016/0378-1119(94)00522-t schema:sameAs https://app.dimensions.ai/details/publication/pub.1022514831
    192 rdf:type schema:CreativeWork
    193 https://doi.org/10.1016/0378-1119(94)00651-8 schema:sameAs https://app.dimensions.ai/details/publication/pub.1037411313
    194 rdf:type schema:CreativeWork
    195 https://doi.org/10.1016/0378-1119(94)00786-r schema:sameAs https://app.dimensions.ai/details/publication/pub.1050674574
    196 rdf:type schema:CreativeWork
    197 https://doi.org/10.1016/0378-1119(95)00147-x schema:sameAs https://app.dimensions.ai/details/publication/pub.1019363042
    198 rdf:type schema:CreativeWork
    199 https://doi.org/10.1073/pnas.82.15.4920 schema:sameAs https://app.dimensions.ai/details/publication/pub.1008941231
    200 rdf:type schema:CreativeWork
    201 https://doi.org/10.1073/pnas.82.19.6497 schema:sameAs https://app.dimensions.ai/details/publication/pub.1037809345
    202 rdf:type schema:CreativeWork
    203 https://doi.org/10.1083/jcb.111.2.567 schema:sameAs https://app.dimensions.ai/details/publication/pub.1033671488
    204 rdf:type schema:CreativeWork
    205 https://doi.org/10.1093/jnci/85.11.897 schema:sameAs https://app.dimensions.ai/details/publication/pub.1059817957
    206 rdf:type schema:CreativeWork
    207 https://doi.org/10.1093/jnci/85.17.1419 schema:sameAs https://app.dimensions.ai/details/publication/pub.1059818126
    208 rdf:type schema:CreativeWork
    209 https://doi.org/10.1097/00019606-199303000-00029 schema:sameAs https://app.dimensions.ai/details/publication/pub.1060293920
    210 rdf:type schema:CreativeWork
    211 https://doi.org/10.1097/00019606-199309000-00009 schema:sameAs https://app.dimensions.ai/details/publication/pub.1005247991
    212 rdf:type schema:CreativeWork
    213 https://doi.org/10.1126/science.2470152 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062539373
    214 rdf:type schema:CreativeWork
    215 https://doi.org/10.1200/jco.1994.12.3.475 schema:sameAs https://app.dimensions.ai/details/publication/pub.1082392048
    216 rdf:type schema:CreativeWork
    217 https://doi.org/10.1200/jco.1994.12.4.725 schema:sameAs https://app.dimensions.ai/details/publication/pub.1082392959
    218 rdf:type schema:CreativeWork
    219 https://www.grid.ac/institutes/grid.412581.b schema:alternateName Witten/Herdecke University
    220 schema:name Institute of Immunology, University Witten/Herdecke, Witten, Germany
    221 rdf:type schema:Organization
    222 https://www.grid.ac/institutes/grid.5949.1 schema:alternateName University of Münster
    223 schema:name Institute of Clinical Chemistry and Laboratory Medicine, Westfälische Wilhelms-Universität Münster, Münster, Germany
    224 rdf:type schema:Organization
     




    Preview window. Press ESC to close (or click here)


    ...