Plant regeneration from leaf protoplasts of apple View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1993-07

AUTHORS

Esmlida Huancaruna Perales, Otto Schieder

ABSTRACT

Protoplasts were isolated from young leaves or etiolated shoot apices. For initiation of divisions the protoplasts were embedded in sodium alginate and cultivated in MS or MI medium supplemented with 2.2 μM BA, 2.6 μM NAA and 2.2 μM 2,4-dichlorophenoxyacetic acid. The protoplasts of all seven lines tested developed to protocalluses at high frequencies. No genotypic differences were observed. When BA was used in combination with NAA in the regeneration experiments, only a few protocalluses (highest frequency 3%) exhibited shoot organogenesis. When BA was replaced with thidiazuron, the percentage of protocalluses that developed shoots increased in two of three tested lines to 7% and 56%, respectively. Shoot development was achieved under light conditions. The shoots were then rooted and transferred into soil. More... »

PAGES

71-76

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf00048465

DOI

http://dx.doi.org/10.1007/bf00048465

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1012786704


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