Anthocyanin production in cultured cells of Aralia cordata Thunb. View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1994-01

AUTHORS

Kazuo Sakamoto, Kumiko Iida, Kimiko Sawamura, Kyoko Hajiro, Yoshihisa Asada, Takafumi Yoshikawa, Tsutomu Furuya

ABSTRACT

High anthocyanin-producing cell lines, which were grown in a dark or in a light-dark regime, were selected from callus cultures initiated from stem and leaf tissues of Aralia cordata Thunb. by small-cell-aggregate selection. To verify the optimum culture conditions for anthocyanin production, cells were tested by changing the various basal media, sucrose concentration and nitrogen source and concentration. Good growth was obtained in the dark on Linsmaier-Skoog's basal medium containing 1.0 mg l-1 2,4-d and 0.1 mg l-1 kinetin, 2% (w/v) sucrose and full strength of nitrogen concentration. However, the highest anthocyanin yield (10.3% dry wt) was obtained in the dark on B5 medium containing 1.0 mg l-1 2,4-d and 0.1 mg l-1 kinetin. Our results suggested that it has became feasible to find the most effective conditions for cell growth and anthocyanin production by optimizations of the nitrogen concentration and the ratio of NH4+ to NO3- in the medium. More... »

PAGES

21-26

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf00048311

DOI

http://dx.doi.org/10.1007/bf00048311

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1031410657


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