Transfer RNA-mediated suppression of stop codons in protoplasts and transgenic plants View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1993-07

AUTHORS

Vera T. C. Carneiro, Georges Pelletier, Ian Small

ABSTRACT

We have developed a simple, rapid and sensitive assay for tRNA gene expression in plant cells. A plant tRNA(Leu) gene was site-specifically mutated to encode each of the three anticodon sequences (CUA, UUA and UCA) that recognize, respectively, the amber, ochre and opal stop codons. The suppression activity of these genes was detected by their ability to restore transient beta-glucuronidase (GUS) expression in tobacco protoplasts electroporated with GUS genes containing premature stop codons. Protoplasts co-electroporated with the amber suppressor tRNA gene and a GUS gene containing a premature amber stop codon showed up to 20-25% of the activity found in protoplasts transfected with the functional control GUS gene. Ochre and opal suppressors presented maximum efficiencies of less than 1%. This system could be adapted to examine transcription, processing or aminoacylation of tRNAs in plant cells. In addition, phenotypically normal, fertile tobacco plants expressing a stably incorporated amber suppressor tRNA gene have been obtained. This suppressor tRNA can be used to transactivate a target gene containing a premature amber stop codon by a factor of at least several hundred-fold. More... »

PAGES

681-690

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf00047408

DOI

http://dx.doi.org/10.1007/bf00047408

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1040119274

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/8343603


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0604", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Genetics", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Amino Acid Sequence", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Anticodon", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Base Sequence", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Codon", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Fabaceae", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Glucuronidase", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Kanamycin Resistance", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Molecular Sequence Data", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Mutation", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Peptide Chain Termination, Translational", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Plants, Genetically Modified", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Plants, Medicinal", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Plants, Toxic", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Protoplasts", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "RNA, Transfer, Leu", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Suppression, Genetic", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Tobacco", 
        "type": "DefinedTerm"
      }, 
      {
        "inDefinedTermSet": "https://www.nlm.nih.gov/mesh/", 
        "name": "Transformation, Genetic", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "name": [
            "Laboratoire de Biologie Cellulaire, INRA, Route de St-Cyr, F-78026, Versailles Cedex, France"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Carneiro", 
        "givenName": "Vera T. C.", 
        "id": "sg:person.0736240322.86", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0736240322.86"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "name": [
            "Laboratoire de Biologie Cellulaire, INRA, Route de St-Cyr, F-78026, Versailles Cedex, France"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Pelletier", 
        "givenName": "Georges", 
        "id": "sg:person.0612016307.03", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0612016307.03"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "name": [
            "Laboratoire de Biologie Cellulaire, INRA, Route de St-Cyr, F-78026, Versailles Cedex, France"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Small", 
        "givenName": "Ian", 
        "id": "sg:person.01313604274.41", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01313604274.41"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "https://doi.org/10.1016/0014-5793(90)80181-h", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1000009788"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00587580", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1005441532", 
          "https://doi.org/10.1007/bf00587580"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00587580", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1005441532", 
          "https://doi.org/10.1007/bf00587580"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0300-9084(87)90181-7", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1005456191"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0300-9084(87)90181-7", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1005456191"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00017457", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1005803602", 
          "https://doi.org/10.1007/bf00017457"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00017457", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1005803602", 
          "https://doi.org/10.1007/bf00017457"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.83.17.6538", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1010088455"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00017725", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1011399693", 
          "https://doi.org/10.1007/bf00017725"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00017725", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1011399693", 
          "https://doi.org/10.1007/bf00017725"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0022-2836(83)80047-3", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1014053718"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/nar/16.11.4777", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1015332519"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/nar/19.6.1349", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1015399349"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0022-2836(86)90362-1", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1015692081"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/337376a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1021278924", 
          "https://doi.org/10.1038/337376a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0014-5793(90)80392-v", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1021903849"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1146/annurev.bi.57.070188.004301", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1022874758"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.86.17.6696", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1022929650"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.87.23.9260", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1023080756"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0168-9452(85)90202-x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1023783252"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0079-6603(08)60006-9", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1025466273"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0003-2697(76)90527-3", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1025529346"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/nar/19.4.707", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1026082213"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00015078", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1026347333", 
          "https://doi.org/10.1007/bf00015078"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00015078", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1026347333", 
          "https://doi.org/10.1007/bf00015078"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1073/pnas.82.2.488", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1026991187"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0076-6879(87)53044-0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1037768034"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00037060", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1039233388", 
          "https://doi.org/10.1007/bf00037060"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00037060", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1039233388", 
          "https://doi.org/10.1007/bf00037060"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/nar/16.7.2841", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1039962593"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00014182", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1042491294", 
          "https://doi.org/10.1007/bf00014182"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00014182", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1042491294", 
          "https://doi.org/10.1007/bf00014182"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1038/333140a0", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1042620457", 
          "https://doi.org/10.1038/333140a0"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1046/j.1365-313x.1992.t01-27-00999.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1044012679"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00028737", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1044432622", 
          "https://doi.org/10.1007/bf00028737"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00028737", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1044432622", 
          "https://doi.org/10.1007/bf00028737"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/mcb.6.7.2674", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1045499058"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1101/gad.5.6.970", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1050574895"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00026790", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1052923688", 
          "https://doi.org/10.1007/bf00026790"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00026790", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1052923688", 
          "https://doi.org/10.1007/bf00026790"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1128/jb.166.3.1022-1027.1986", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1062714920"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/j.1460-2075.1992.tb05510.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1076154142"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/j.1460-2075.1992.tb05172.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1076988923"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/j.1460-2075.1992.tb05243.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1077037140"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/j.1460-2075.1984.tb01934.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1077183666"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/j.1460-2075.1984.tb02201.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1077183716"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/j.1460-2075.1991.tb07717.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1077325071"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/j.1460-2075.1990.tb08163.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1078428127"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1078715005", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/j.1460-2075.1989.tb08564.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1079222647"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1079417138", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1079492136", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://app.dimensions.ai/details/publication/pub.1079528125", 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/j.1460-2075.1988.tb03107.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1079576373"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/j.1460-2075.1988.tb03267.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1079613955"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/j.1460-2075.1987.tb02730.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1079687101"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1002/j.1460-2075.1987.tb04715.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1079787948"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1093/oxfordjournals.jhered.a110875", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1083620322"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "1993-07", 
    "datePublishedReg": "1993-07-01", 
    "description": "We have developed a simple, rapid and sensitive assay for tRNA gene expression in plant cells. A plant tRNA(Leu) gene was site-specifically mutated to encode each of the three anticodon sequences (CUA, UUA and UCA) that recognize, respectively, the amber, ochre and opal stop codons. The suppression activity of these genes was detected by their ability to restore transient beta-glucuronidase (GUS) expression in tobacco protoplasts electroporated with GUS genes containing premature stop codons. Protoplasts co-electroporated with the amber suppressor tRNA gene and a GUS gene containing a premature amber stop codon showed up to 20-25% of the activity found in protoplasts transfected with the functional control GUS gene. Ochre and opal suppressors presented maximum efficiencies of less than 1%. This system could be adapted to examine transcription, processing or aminoacylation of tRNAs in plant cells. In addition, phenotypically normal, fertile tobacco plants expressing a stably incorporated amber suppressor tRNA gene have been obtained. This suppressor tRNA can be used to transactivate a target gene containing a premature amber stop codon by a factor of at least several hundred-fold.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1007/bf00047408", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": false, 
    "isPartOf": [
      {
        "id": "sg:journal.1101246", 
        "issn": [
          "0167-4412", 
          "1573-5028"
        ], 
        "name": "Plant Molecular Biology", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "4", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "22"
      }
    ], 
    "name": "Transfer RNA-mediated suppression of stop codons in protoplasts and transgenic plants", 
    "pagination": "681-690", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "bf1d26b59544e11f4490abae5589908d2b7735d95766c48a213cfec67aa38b75"
        ]
      }, 
      {
        "name": "pubmed_id", 
        "type": "PropertyValue", 
        "value": [
          "8343603"
        ]
      }, 
      {
        "name": "nlm_unique_id", 
        "type": "PropertyValue", 
        "value": [
          "9106343"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1007/bf00047408"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1040119274"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1007/bf00047408", 
      "https://app.dimensions.ai/details/publication/pub.1040119274"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-11T13:53", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000371_0000000371/records_130808_00000003.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "http://link.springer.com/10.1007/BF00047408"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/bf00047408'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/bf00047408'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/bf00047408'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/bf00047408'


 

This table displays all metadata directly associated to this object as RDF triples.

311 TRIPLES      21 PREDICATES      96 URIs      39 LITERALS      27 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1007/bf00047408 schema:about N028c7b53d0bf46e2a95ccc11a8100ea9
2 N2814b7f112a64860bd4e49088d1ddf28
3 N33075c8c58794da09b7851e2b5aebaba
4 N34ff204fd53a4843803875ad2c874de2
5 N3c9c4ce91a0f48599812c0814c89ee34
6 N4d7b6644cc6f4e2f9b08e6c43a79548e
7 N4fff77b9a7084b98806755b654467ef8
8 N6193f64352fd44128c9c7779d214c818
9 N773cd1b78fcd45b5bb871843cbae723d
10 N88f01c59fde64ef59480f70057e35ab4
11 N8b6d7876ea1f4ad1a9fa75153152cd9d
12 Na26e16c786be48908bef7950b4120a0b
13 Nc1b07be3aebe45b5bbb1ca79e486311f
14 Ncac9a1f1ec3e46fb8969a2099ec74276
15 Ncb4b5961e48b45688eba1b6ba51b354c
16 Nd23b9b889f144ce0bf51a19653fa84d4
17 Ne896fb56714c4d63acfae3cd6be410b7
18 Nfb39c3bfda7d4e878e2d120a1f7ea169
19 anzsrc-for:06
20 anzsrc-for:0604
21 schema:author N947ed04fbf184b819a10e922084a409b
22 schema:citation sg:pub.10.1007/bf00014182
23 sg:pub.10.1007/bf00015078
24 sg:pub.10.1007/bf00017457
25 sg:pub.10.1007/bf00017725
26 sg:pub.10.1007/bf00026790
27 sg:pub.10.1007/bf00028737
28 sg:pub.10.1007/bf00037060
29 sg:pub.10.1007/bf00587580
30 sg:pub.10.1038/333140a0
31 sg:pub.10.1038/337376a0
32 https://app.dimensions.ai/details/publication/pub.1078715005
33 https://app.dimensions.ai/details/publication/pub.1079417138
34 https://app.dimensions.ai/details/publication/pub.1079492136
35 https://app.dimensions.ai/details/publication/pub.1079528125
36 https://doi.org/10.1002/j.1460-2075.1984.tb01934.x
37 https://doi.org/10.1002/j.1460-2075.1984.tb02201.x
38 https://doi.org/10.1002/j.1460-2075.1987.tb02730.x
39 https://doi.org/10.1002/j.1460-2075.1987.tb04715.x
40 https://doi.org/10.1002/j.1460-2075.1988.tb03107.x
41 https://doi.org/10.1002/j.1460-2075.1988.tb03267.x
42 https://doi.org/10.1002/j.1460-2075.1989.tb08564.x
43 https://doi.org/10.1002/j.1460-2075.1990.tb08163.x
44 https://doi.org/10.1002/j.1460-2075.1991.tb07717.x
45 https://doi.org/10.1002/j.1460-2075.1992.tb05172.x
46 https://doi.org/10.1002/j.1460-2075.1992.tb05243.x
47 https://doi.org/10.1002/j.1460-2075.1992.tb05510.x
48 https://doi.org/10.1016/0003-2697(76)90527-3
49 https://doi.org/10.1016/0014-5793(90)80181-h
50 https://doi.org/10.1016/0014-5793(90)80392-v
51 https://doi.org/10.1016/0022-2836(86)90362-1
52 https://doi.org/10.1016/0076-6879(87)53044-0
53 https://doi.org/10.1016/0168-9452(85)90202-x
54 https://doi.org/10.1016/0300-9084(87)90181-7
55 https://doi.org/10.1016/s0022-2836(83)80047-3
56 https://doi.org/10.1016/s0079-6603(08)60006-9
57 https://doi.org/10.1046/j.1365-313x.1992.t01-27-00999.x
58 https://doi.org/10.1073/pnas.82.2.488
59 https://doi.org/10.1073/pnas.83.17.6538
60 https://doi.org/10.1073/pnas.86.17.6696
61 https://doi.org/10.1073/pnas.87.23.9260
62 https://doi.org/10.1093/nar/16.11.4777
63 https://doi.org/10.1093/nar/16.7.2841
64 https://doi.org/10.1093/nar/19.4.707
65 https://doi.org/10.1093/nar/19.6.1349
66 https://doi.org/10.1093/oxfordjournals.jhered.a110875
67 https://doi.org/10.1101/gad.5.6.970
68 https://doi.org/10.1128/jb.166.3.1022-1027.1986
69 https://doi.org/10.1128/mcb.6.7.2674
70 https://doi.org/10.1146/annurev.bi.57.070188.004301
71 schema:datePublished 1993-07
72 schema:datePublishedReg 1993-07-01
73 schema:description We have developed a simple, rapid and sensitive assay for tRNA gene expression in plant cells. A plant tRNA(Leu) gene was site-specifically mutated to encode each of the three anticodon sequences (CUA, UUA and UCA) that recognize, respectively, the amber, ochre and opal stop codons. The suppression activity of these genes was detected by their ability to restore transient beta-glucuronidase (GUS) expression in tobacco protoplasts electroporated with GUS genes containing premature stop codons. Protoplasts co-electroporated with the amber suppressor tRNA gene and a GUS gene containing a premature amber stop codon showed up to 20-25% of the activity found in protoplasts transfected with the functional control GUS gene. Ochre and opal suppressors presented maximum efficiencies of less than 1%. This system could be adapted to examine transcription, processing or aminoacylation of tRNAs in plant cells. In addition, phenotypically normal, fertile tobacco plants expressing a stably incorporated amber suppressor tRNA gene have been obtained. This suppressor tRNA can be used to transactivate a target gene containing a premature amber stop codon by a factor of at least several hundred-fold.
74 schema:genre research_article
75 schema:inLanguage en
76 schema:isAccessibleForFree false
77 schema:isPartOf N2924b0ba821c44a09c2c709ddfa3fe99
78 Nbf53ec9b90bb46b2892a90803a952d8f
79 sg:journal.1101246
80 schema:name Transfer RNA-mediated suppression of stop codons in protoplasts and transgenic plants
81 schema:pagination 681-690
82 schema:productId N67d388631a804b5da38054e91e72e9f8
83 N77f83bd96d0a4ebe8312bd4ebf106459
84 N7ccc495695184acd9260f6155c3f8aaf
85 N8c1746bff3ae44b1ae72c0813bf0f9dc
86 Ncf0e76dd99824f5bb4403ba7ff793cbf
87 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040119274
88 https://doi.org/10.1007/bf00047408
89 schema:sdDatePublished 2019-04-11T13:53
90 schema:sdLicense https://scigraph.springernature.com/explorer/license/
91 schema:sdPublisher Ncccdce4765f14f5c8f203e713e21e7b3
92 schema:url http://link.springer.com/10.1007/BF00047408
93 sgo:license sg:explorer/license/
94 sgo:sdDataset articles
95 rdf:type schema:ScholarlyArticle
96 N028c7b53d0bf46e2a95ccc11a8100ea9 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
97 schema:name Base Sequence
98 rdf:type schema:DefinedTerm
99 N0c59d94012d941398a5334bdc438a570 schema:name Laboratoire de Biologie Cellulaire, INRA, Route de St-Cyr, F-78026, Versailles Cedex, France
100 rdf:type schema:Organization
101 N14815bfcbc6d4865b9e7d64f9a00b980 schema:name Laboratoire de Biologie Cellulaire, INRA, Route de St-Cyr, F-78026, Versailles Cedex, France
102 rdf:type schema:Organization
103 N2814b7f112a64860bd4e49088d1ddf28 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
104 schema:name Fabaceae
105 rdf:type schema:DefinedTerm
106 N2924b0ba821c44a09c2c709ddfa3fe99 schema:volumeNumber 22
107 rdf:type schema:PublicationVolume
108 N33075c8c58794da09b7851e2b5aebaba schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
109 schema:name Amino Acid Sequence
110 rdf:type schema:DefinedTerm
111 N34ff204fd53a4843803875ad2c874de2 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
112 schema:name Plants, Toxic
113 rdf:type schema:DefinedTerm
114 N3c9c4ce91a0f48599812c0814c89ee34 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
115 schema:name Plants, Genetically Modified
116 rdf:type schema:DefinedTerm
117 N4d7b6644cc6f4e2f9b08e6c43a79548e schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
118 schema:name Glucuronidase
119 rdf:type schema:DefinedTerm
120 N4fff77b9a7084b98806755b654467ef8 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
121 schema:name Plants, Medicinal
122 rdf:type schema:DefinedTerm
123 N6193f64352fd44128c9c7779d214c818 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
124 schema:name Protoplasts
125 rdf:type schema:DefinedTerm
126 N67d388631a804b5da38054e91e72e9f8 schema:name readcube_id
127 schema:value bf1d26b59544e11f4490abae5589908d2b7735d95766c48a213cfec67aa38b75
128 rdf:type schema:PropertyValue
129 N766ad78415bb430f961fc64fc88bb2dc schema:name Laboratoire de Biologie Cellulaire, INRA, Route de St-Cyr, F-78026, Versailles Cedex, France
130 rdf:type schema:Organization
131 N773cd1b78fcd45b5bb871843cbae723d schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
132 schema:name Transformation, Genetic
133 rdf:type schema:DefinedTerm
134 N77f83bd96d0a4ebe8312bd4ebf106459 schema:name doi
135 schema:value 10.1007/bf00047408
136 rdf:type schema:PropertyValue
137 N7ccc495695184acd9260f6155c3f8aaf schema:name nlm_unique_id
138 schema:value 9106343
139 rdf:type schema:PropertyValue
140 N88f01c59fde64ef59480f70057e35ab4 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
141 schema:name Kanamycin Resistance
142 rdf:type schema:DefinedTerm
143 N8b6d7876ea1f4ad1a9fa75153152cd9d schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
144 schema:name Codon
145 rdf:type schema:DefinedTerm
146 N8c1746bff3ae44b1ae72c0813bf0f9dc schema:name dimensions_id
147 schema:value pub.1040119274
148 rdf:type schema:PropertyValue
149 N947ed04fbf184b819a10e922084a409b rdf:first sg:person.0736240322.86
150 rdf:rest Nec990200e6cc4c618471ba58c121b82c
151 Na26e16c786be48908bef7950b4120a0b schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
152 schema:name Tobacco
153 rdf:type schema:DefinedTerm
154 Nbf53ec9b90bb46b2892a90803a952d8f schema:issueNumber 4
155 rdf:type schema:PublicationIssue
156 Nc1b07be3aebe45b5bbb1ca79e486311f schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
157 schema:name Anticodon
158 rdf:type schema:DefinedTerm
159 Ncac9a1f1ec3e46fb8969a2099ec74276 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
160 schema:name Mutation
161 rdf:type schema:DefinedTerm
162 Ncb4b5961e48b45688eba1b6ba51b354c schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
163 schema:name RNA, Transfer, Leu
164 rdf:type schema:DefinedTerm
165 Ncccdce4765f14f5c8f203e713e21e7b3 schema:name Springer Nature - SN SciGraph project
166 rdf:type schema:Organization
167 Ncf0e76dd99824f5bb4403ba7ff793cbf schema:name pubmed_id
168 schema:value 8343603
169 rdf:type schema:PropertyValue
170 Nd23b9b889f144ce0bf51a19653fa84d4 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
171 schema:name Molecular Sequence Data
172 rdf:type schema:DefinedTerm
173 Nd274eb538aa64c40ad210a5af443b41a rdf:first sg:person.01313604274.41
174 rdf:rest rdf:nil
175 Ne896fb56714c4d63acfae3cd6be410b7 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
176 schema:name Peptide Chain Termination, Translational
177 rdf:type schema:DefinedTerm
178 Nec990200e6cc4c618471ba58c121b82c rdf:first sg:person.0612016307.03
179 rdf:rest Nd274eb538aa64c40ad210a5af443b41a
180 Nfb39c3bfda7d4e878e2d120a1f7ea169 schema:inDefinedTermSet https://www.nlm.nih.gov/mesh/
181 schema:name Suppression, Genetic
182 rdf:type schema:DefinedTerm
183 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
184 schema:name Biological Sciences
185 rdf:type schema:DefinedTerm
186 anzsrc-for:0604 schema:inDefinedTermSet anzsrc-for:
187 schema:name Genetics
188 rdf:type schema:DefinedTerm
189 sg:journal.1101246 schema:issn 0167-4412
190 1573-5028
191 schema:name Plant Molecular Biology
192 rdf:type schema:Periodical
193 sg:person.01313604274.41 schema:affiliation N0c59d94012d941398a5334bdc438a570
194 schema:familyName Small
195 schema:givenName Ian
196 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01313604274.41
197 rdf:type schema:Person
198 sg:person.0612016307.03 schema:affiliation N14815bfcbc6d4865b9e7d64f9a00b980
199 schema:familyName Pelletier
200 schema:givenName Georges
201 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0612016307.03
202 rdf:type schema:Person
203 sg:person.0736240322.86 schema:affiliation N766ad78415bb430f961fc64fc88bb2dc
204 schema:familyName Carneiro
205 schema:givenName Vera T. C.
206 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0736240322.86
207 rdf:type schema:Person
208 sg:pub.10.1007/bf00014182 schema:sameAs https://app.dimensions.ai/details/publication/pub.1042491294
209 https://doi.org/10.1007/bf00014182
210 rdf:type schema:CreativeWork
211 sg:pub.10.1007/bf00015078 schema:sameAs https://app.dimensions.ai/details/publication/pub.1026347333
212 https://doi.org/10.1007/bf00015078
213 rdf:type schema:CreativeWork
214 sg:pub.10.1007/bf00017457 schema:sameAs https://app.dimensions.ai/details/publication/pub.1005803602
215 https://doi.org/10.1007/bf00017457
216 rdf:type schema:CreativeWork
217 sg:pub.10.1007/bf00017725 schema:sameAs https://app.dimensions.ai/details/publication/pub.1011399693
218 https://doi.org/10.1007/bf00017725
219 rdf:type schema:CreativeWork
220 sg:pub.10.1007/bf00026790 schema:sameAs https://app.dimensions.ai/details/publication/pub.1052923688
221 https://doi.org/10.1007/bf00026790
222 rdf:type schema:CreativeWork
223 sg:pub.10.1007/bf00028737 schema:sameAs https://app.dimensions.ai/details/publication/pub.1044432622
224 https://doi.org/10.1007/bf00028737
225 rdf:type schema:CreativeWork
226 sg:pub.10.1007/bf00037060 schema:sameAs https://app.dimensions.ai/details/publication/pub.1039233388
227 https://doi.org/10.1007/bf00037060
228 rdf:type schema:CreativeWork
229 sg:pub.10.1007/bf00587580 schema:sameAs https://app.dimensions.ai/details/publication/pub.1005441532
230 https://doi.org/10.1007/bf00587580
231 rdf:type schema:CreativeWork
232 sg:pub.10.1038/333140a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1042620457
233 https://doi.org/10.1038/333140a0
234 rdf:type schema:CreativeWork
235 sg:pub.10.1038/337376a0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1021278924
236 https://doi.org/10.1038/337376a0
237 rdf:type schema:CreativeWork
238 https://app.dimensions.ai/details/publication/pub.1078715005 schema:CreativeWork
239 https://app.dimensions.ai/details/publication/pub.1079417138 schema:CreativeWork
240 https://app.dimensions.ai/details/publication/pub.1079492136 schema:CreativeWork
241 https://app.dimensions.ai/details/publication/pub.1079528125 schema:CreativeWork
242 https://doi.org/10.1002/j.1460-2075.1984.tb01934.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1077183666
243 rdf:type schema:CreativeWork
244 https://doi.org/10.1002/j.1460-2075.1984.tb02201.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1077183716
245 rdf:type schema:CreativeWork
246 https://doi.org/10.1002/j.1460-2075.1987.tb02730.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1079687101
247 rdf:type schema:CreativeWork
248 https://doi.org/10.1002/j.1460-2075.1987.tb04715.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1079787948
249 rdf:type schema:CreativeWork
250 https://doi.org/10.1002/j.1460-2075.1988.tb03107.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1079576373
251 rdf:type schema:CreativeWork
252 https://doi.org/10.1002/j.1460-2075.1988.tb03267.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1079613955
253 rdf:type schema:CreativeWork
254 https://doi.org/10.1002/j.1460-2075.1989.tb08564.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1079222647
255 rdf:type schema:CreativeWork
256 https://doi.org/10.1002/j.1460-2075.1990.tb08163.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1078428127
257 rdf:type schema:CreativeWork
258 https://doi.org/10.1002/j.1460-2075.1991.tb07717.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1077325071
259 rdf:type schema:CreativeWork
260 https://doi.org/10.1002/j.1460-2075.1992.tb05172.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1076988923
261 rdf:type schema:CreativeWork
262 https://doi.org/10.1002/j.1460-2075.1992.tb05243.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1077037140
263 rdf:type schema:CreativeWork
264 https://doi.org/10.1002/j.1460-2075.1992.tb05510.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1076154142
265 rdf:type schema:CreativeWork
266 https://doi.org/10.1016/0003-2697(76)90527-3 schema:sameAs https://app.dimensions.ai/details/publication/pub.1025529346
267 rdf:type schema:CreativeWork
268 https://doi.org/10.1016/0014-5793(90)80181-h schema:sameAs https://app.dimensions.ai/details/publication/pub.1000009788
269 rdf:type schema:CreativeWork
270 https://doi.org/10.1016/0014-5793(90)80392-v schema:sameAs https://app.dimensions.ai/details/publication/pub.1021903849
271 rdf:type schema:CreativeWork
272 https://doi.org/10.1016/0022-2836(86)90362-1 schema:sameAs https://app.dimensions.ai/details/publication/pub.1015692081
273 rdf:type schema:CreativeWork
274 https://doi.org/10.1016/0076-6879(87)53044-0 schema:sameAs https://app.dimensions.ai/details/publication/pub.1037768034
275 rdf:type schema:CreativeWork
276 https://doi.org/10.1016/0168-9452(85)90202-x schema:sameAs https://app.dimensions.ai/details/publication/pub.1023783252
277 rdf:type schema:CreativeWork
278 https://doi.org/10.1016/0300-9084(87)90181-7 schema:sameAs https://app.dimensions.ai/details/publication/pub.1005456191
279 rdf:type schema:CreativeWork
280 https://doi.org/10.1016/s0022-2836(83)80047-3 schema:sameAs https://app.dimensions.ai/details/publication/pub.1014053718
281 rdf:type schema:CreativeWork
282 https://doi.org/10.1016/s0079-6603(08)60006-9 schema:sameAs https://app.dimensions.ai/details/publication/pub.1025466273
283 rdf:type schema:CreativeWork
284 https://doi.org/10.1046/j.1365-313x.1992.t01-27-00999.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1044012679
285 rdf:type schema:CreativeWork
286 https://doi.org/10.1073/pnas.82.2.488 schema:sameAs https://app.dimensions.ai/details/publication/pub.1026991187
287 rdf:type schema:CreativeWork
288 https://doi.org/10.1073/pnas.83.17.6538 schema:sameAs https://app.dimensions.ai/details/publication/pub.1010088455
289 rdf:type schema:CreativeWork
290 https://doi.org/10.1073/pnas.86.17.6696 schema:sameAs https://app.dimensions.ai/details/publication/pub.1022929650
291 rdf:type schema:CreativeWork
292 https://doi.org/10.1073/pnas.87.23.9260 schema:sameAs https://app.dimensions.ai/details/publication/pub.1023080756
293 rdf:type schema:CreativeWork
294 https://doi.org/10.1093/nar/16.11.4777 schema:sameAs https://app.dimensions.ai/details/publication/pub.1015332519
295 rdf:type schema:CreativeWork
296 https://doi.org/10.1093/nar/16.7.2841 schema:sameAs https://app.dimensions.ai/details/publication/pub.1039962593
297 rdf:type schema:CreativeWork
298 https://doi.org/10.1093/nar/19.4.707 schema:sameAs https://app.dimensions.ai/details/publication/pub.1026082213
299 rdf:type schema:CreativeWork
300 https://doi.org/10.1093/nar/19.6.1349 schema:sameAs https://app.dimensions.ai/details/publication/pub.1015399349
301 rdf:type schema:CreativeWork
302 https://doi.org/10.1093/oxfordjournals.jhered.a110875 schema:sameAs https://app.dimensions.ai/details/publication/pub.1083620322
303 rdf:type schema:CreativeWork
304 https://doi.org/10.1101/gad.5.6.970 schema:sameAs https://app.dimensions.ai/details/publication/pub.1050574895
305 rdf:type schema:CreativeWork
306 https://doi.org/10.1128/jb.166.3.1022-1027.1986 schema:sameAs https://app.dimensions.ai/details/publication/pub.1062714920
307 rdf:type schema:CreativeWork
308 https://doi.org/10.1128/mcb.6.7.2674 schema:sameAs https://app.dimensions.ai/details/publication/pub.1045499058
309 rdf:type schema:CreativeWork
310 https://doi.org/10.1146/annurev.bi.57.070188.004301 schema:sameAs https://app.dimensions.ai/details/publication/pub.1022874758
311 rdf:type schema:CreativeWork
 




Preview window. Press ESC to close (or click here)


...