Ontology type: schema:ScholarlyArticle
1993-07
AUTHORSVera T. C. Carneiro, Georges Pelletier, Ian Small
ABSTRACTWe have developed a simple, rapid and sensitive assay for tRNA gene expression in plant cells. A plant tRNA(Leu) gene was site-specifically mutated to encode each of the three anticodon sequences (CUA, UUA and UCA) that recognize, respectively, the amber, ochre and opal stop codons. The suppression activity of these genes was detected by their ability to restore transient beta-glucuronidase (GUS) expression in tobacco protoplasts electroporated with GUS genes containing premature stop codons. Protoplasts co-electroporated with the amber suppressor tRNA gene and a GUS gene containing a premature amber stop codon showed up to 20-25% of the activity found in protoplasts transfected with the functional control GUS gene. Ochre and opal suppressors presented maximum efficiencies of less than 1%. This system could be adapted to examine transcription, processing or aminoacylation of tRNAs in plant cells. In addition, phenotypically normal, fertile tobacco plants expressing a stably incorporated amber suppressor tRNA gene have been obtained. This suppressor tRNA can be used to transactivate a target gene containing a premature amber stop codon by a factor of at least several hundred-fold. More... »
PAGES681-690
http://scigraph.springernature.com/pub.10.1007/bf00047408
DOIhttp://dx.doi.org/10.1007/bf00047408
DIMENSIONShttps://app.dimensions.ai/details/publication/pub.1040119274
PUBMEDhttps://www.ncbi.nlm.nih.gov/pubmed/8343603
JSON-LD is the canonical representation for SciGraph data.
TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT
[
{
"@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json",
"about": [
{
"id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0604",
"inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/",
"name": "Genetics",
"type": "DefinedTerm"
},
{
"id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06",
"inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/",
"name": "Biological Sciences",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Amino Acid Sequence",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Anticodon",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Base Sequence",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Codon",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Fabaceae",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Glucuronidase",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Kanamycin Resistance",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Molecular Sequence Data",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Mutation",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Peptide Chain Termination, Translational",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Plants, Genetically Modified",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Plants, Medicinal",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Plants, Toxic",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Protoplasts",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "RNA, Transfer, Leu",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Suppression, Genetic",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Tobacco",
"type": "DefinedTerm"
},
{
"inDefinedTermSet": "https://www.nlm.nih.gov/mesh/",
"name": "Transformation, Genetic",
"type": "DefinedTerm"
}
],
"author": [
{
"affiliation": {
"name": [
"Laboratoire de Biologie Cellulaire, INRA, Route de St-Cyr, F-78026, Versailles Cedex, France"
],
"type": "Organization"
},
"familyName": "Carneiro",
"givenName": "Vera T. C.",
"id": "sg:person.0736240322.86",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0736240322.86"
],
"type": "Person"
},
{
"affiliation": {
"name": [
"Laboratoire de Biologie Cellulaire, INRA, Route de St-Cyr, F-78026, Versailles Cedex, France"
],
"type": "Organization"
},
"familyName": "Pelletier",
"givenName": "Georges",
"id": "sg:person.0612016307.03",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.0612016307.03"
],
"type": "Person"
},
{
"affiliation": {
"name": [
"Laboratoire de Biologie Cellulaire, INRA, Route de St-Cyr, F-78026, Versailles Cedex, France"
],
"type": "Organization"
},
"familyName": "Small",
"givenName": "Ian",
"id": "sg:person.01313604274.41",
"sameAs": [
"https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01313604274.41"
],
"type": "Person"
}
],
"citation": [
{
"id": "https://doi.org/10.1016/0014-5793(90)80181-h",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1000009788"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/bf00587580",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1005441532",
"https://doi.org/10.1007/bf00587580"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/bf00587580",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1005441532",
"https://doi.org/10.1007/bf00587580"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/0300-9084(87)90181-7",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1005456191"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/0300-9084(87)90181-7",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1005456191"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/bf00017457",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1005803602",
"https://doi.org/10.1007/bf00017457"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/bf00017457",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1005803602",
"https://doi.org/10.1007/bf00017457"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1073/pnas.83.17.6538",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1010088455"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/bf00017725",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1011399693",
"https://doi.org/10.1007/bf00017725"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/bf00017725",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1011399693",
"https://doi.org/10.1007/bf00017725"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/s0022-2836(83)80047-3",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1014053718"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1093/nar/16.11.4777",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1015332519"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1093/nar/19.6.1349",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1015399349"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/0022-2836(86)90362-1",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1015692081"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/337376a0",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1021278924",
"https://doi.org/10.1038/337376a0"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/0014-5793(90)80392-v",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1021903849"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1146/annurev.bi.57.070188.004301",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1022874758"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1073/pnas.86.17.6696",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1022929650"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1073/pnas.87.23.9260",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1023080756"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/0168-9452(85)90202-x",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1023783252"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/s0079-6603(08)60006-9",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1025466273"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/0003-2697(76)90527-3",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1025529346"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1093/nar/19.4.707",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1026082213"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/bf00015078",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1026347333",
"https://doi.org/10.1007/bf00015078"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/bf00015078",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1026347333",
"https://doi.org/10.1007/bf00015078"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1073/pnas.82.2.488",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1026991187"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1016/0076-6879(87)53044-0",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1037768034"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/bf00037060",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1039233388",
"https://doi.org/10.1007/bf00037060"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/bf00037060",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1039233388",
"https://doi.org/10.1007/bf00037060"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1093/nar/16.7.2841",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1039962593"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/bf00014182",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1042491294",
"https://doi.org/10.1007/bf00014182"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/bf00014182",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1042491294",
"https://doi.org/10.1007/bf00014182"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1038/333140a0",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1042620457",
"https://doi.org/10.1038/333140a0"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1046/j.1365-313x.1992.t01-27-00999.x",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1044012679"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/bf00028737",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1044432622",
"https://doi.org/10.1007/bf00028737"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/bf00028737",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1044432622",
"https://doi.org/10.1007/bf00028737"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1128/mcb.6.7.2674",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1045499058"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1101/gad.5.6.970",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1050574895"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/bf00026790",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1052923688",
"https://doi.org/10.1007/bf00026790"
],
"type": "CreativeWork"
},
{
"id": "sg:pub.10.1007/bf00026790",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1052923688",
"https://doi.org/10.1007/bf00026790"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1128/jb.166.3.1022-1027.1986",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1062714920"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1002/j.1460-2075.1992.tb05510.x",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1076154142"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1002/j.1460-2075.1992.tb05172.x",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1076988923"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1002/j.1460-2075.1992.tb05243.x",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1077037140"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1002/j.1460-2075.1984.tb01934.x",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1077183666"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1002/j.1460-2075.1984.tb02201.x",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1077183716"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1002/j.1460-2075.1991.tb07717.x",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1077325071"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1002/j.1460-2075.1990.tb08163.x",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1078428127"
],
"type": "CreativeWork"
},
{
"id": "https://app.dimensions.ai/details/publication/pub.1078715005",
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1002/j.1460-2075.1989.tb08564.x",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1079222647"
],
"type": "CreativeWork"
},
{
"id": "https://app.dimensions.ai/details/publication/pub.1079417138",
"type": "CreativeWork"
},
{
"id": "https://app.dimensions.ai/details/publication/pub.1079492136",
"type": "CreativeWork"
},
{
"id": "https://app.dimensions.ai/details/publication/pub.1079528125",
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1002/j.1460-2075.1988.tb03107.x",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1079576373"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1002/j.1460-2075.1988.tb03267.x",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1079613955"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1002/j.1460-2075.1987.tb02730.x",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1079687101"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1002/j.1460-2075.1987.tb04715.x",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1079787948"
],
"type": "CreativeWork"
},
{
"id": "https://doi.org/10.1093/oxfordjournals.jhered.a110875",
"sameAs": [
"https://app.dimensions.ai/details/publication/pub.1083620322"
],
"type": "CreativeWork"
}
],
"datePublished": "1993-07",
"datePublishedReg": "1993-07-01",
"description": "We have developed a simple, rapid and sensitive assay for tRNA gene expression in plant cells. A plant tRNA(Leu) gene was site-specifically mutated to encode each of the three anticodon sequences (CUA, UUA and UCA) that recognize, respectively, the amber, ochre and opal stop codons. The suppression activity of these genes was detected by their ability to restore transient beta-glucuronidase (GUS) expression in tobacco protoplasts electroporated with GUS genes containing premature stop codons. Protoplasts co-electroporated with the amber suppressor tRNA gene and a GUS gene containing a premature amber stop codon showed up to 20-25% of the activity found in protoplasts transfected with the functional control GUS gene. Ochre and opal suppressors presented maximum efficiencies of less than 1%. This system could be adapted to examine transcription, processing or aminoacylation of tRNAs in plant cells. In addition, phenotypically normal, fertile tobacco plants expressing a stably incorporated amber suppressor tRNA gene have been obtained. This suppressor tRNA can be used to transactivate a target gene containing a premature amber stop codon by a factor of at least several hundred-fold.",
"genre": "research_article",
"id": "sg:pub.10.1007/bf00047408",
"inLanguage": [
"en"
],
"isAccessibleForFree": false,
"isPartOf": [
{
"id": "sg:journal.1101246",
"issn": [
"0167-4412",
"1573-5028"
],
"name": "Plant Molecular Biology",
"type": "Periodical"
},
{
"issueNumber": "4",
"type": "PublicationIssue"
},
{
"type": "PublicationVolume",
"volumeNumber": "22"
}
],
"name": "Transfer RNA-mediated suppression of stop codons in protoplasts and transgenic plants",
"pagination": "681-690",
"productId": [
{
"name": "readcube_id",
"type": "PropertyValue",
"value": [
"bf1d26b59544e11f4490abae5589908d2b7735d95766c48a213cfec67aa38b75"
]
},
{
"name": "pubmed_id",
"type": "PropertyValue",
"value": [
"8343603"
]
},
{
"name": "nlm_unique_id",
"type": "PropertyValue",
"value": [
"9106343"
]
},
{
"name": "doi",
"type": "PropertyValue",
"value": [
"10.1007/bf00047408"
]
},
{
"name": "dimensions_id",
"type": "PropertyValue",
"value": [
"pub.1040119274"
]
}
],
"sameAs": [
"https://doi.org/10.1007/bf00047408",
"https://app.dimensions.ai/details/publication/pub.1040119274"
],
"sdDataset": "articles",
"sdDatePublished": "2019-04-11T13:53",
"sdLicense": "https://scigraph.springernature.com/explorer/license/",
"sdPublisher": {
"name": "Springer Nature - SN SciGraph project",
"type": "Organization"
},
"sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000371_0000000371/records_130808_00000003.jsonl",
"type": "ScholarlyArticle",
"url": "http://link.springer.com/10.1007/BF00047408"
}
]
Download the RDF metadata as: json-ld nt turtle xml License info
JSON-LD is a popular format for linked data which is fully compatible with JSON.
curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/bf00047408'
N-Triples is a line-based linked data format ideal for batch operations.
curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/bf00047408'
Turtle is a human-readable linked data format.
curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/bf00047408'
RDF/XML is a standard XML format for linked data.
curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/bf00047408'
This table displays all metadata directly associated to this object as RDF triples.
311 TRIPLES
21 PREDICATES
96 URIs
39 LITERALS
27 BLANK NODES