Influence of auxin type and concentration on peanut somatic embryogenesis View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1994-03

AUTHORS

Charleen M. Baker, Hazel Y. Wetzstein

ABSTRACT

Somatic embryogenesis in peanut (Arachis hypogaea L.) using immature cotyledonary explants was induced on a wide range of 2,4-dichlorophenoxyacetic acid (2,4-D) (5 to 60mg l−1) and naphthaleneacetic acid (NAA) (20 to 50 mg l−1) levels. Percent embryogenesis ranged from 31 to 94%. As auxin level increased in induction medium, percent embryogenesis decreased and was associated with browning of explants. However, with higher 2,4-D induction levels (40 mg l−1 and over), embryogenic explants had dense masses of embryogenic areas and repetitive embryogenesis was enhanced. Higher auxin concentrations during induction decreased precocious germination of embryos, but had no marked effect on somatic embryo morphology. The use of 2,4-D compared to NAA in the induction medium resulted in greater per cent embryogenesis and mean number of embryos. Embryos induced on NAA were harder, less pliant, and less succulent; cultures exhibited more extensive root development and nonembryogenic callus proliferation. More... »

PAGES

361-368

References to SciGraph publications

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf00046094

DOI

http://dx.doi.org/10.1007/bf00046094

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1029365871


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