Micropropagation of parsley through axillary shoot proliferation View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1996-01

AUTHORS

J. L. Vandemoortele, J. P. Billard, J. Boucaud, Th. Gaspar

ABSTRACT

A micropropagation protocol of parsley,Petroselinum crispum (Mill.) Nyman (curled type) has been developed. Surface-sterilized axillary buds cultured on Murashige and Skoog medium supplemented with benzyladenine, kinetin, or thidiazuron developed axillary shoots (rosettes). Kinetin resulted in only a low proliferation rate. The concentrations of thidiazuron or benzyladenine that were optimal for shoot proliferation, resulted in shoots with a low capability to root. During the rooting treatment, these shoots showed wilting signs. Rooting was increased significantly by using a two-week inductive stage with 2.5 μM naphthaleneacetic acid directly followed by acclimatization. Two proliferation media (5 μM benzyladenine and 0.5 μM naphthaleneacetic acid or 5 μM kinetin and 2.5 μM naphthaleneacetic acid) resulted in moderate proliferation but produced shoots that were easy-to-root. These media have been tested by repeated axillary proliferation on the same medium. The medium with 5 μM benzyladenine and 0.5 μM naphthaleneacetic acid was optimal. More... »

PAGES

25-30

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf00045909

DOI

http://dx.doi.org/10.1007/bf00045909

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1016796773


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