Preparation of a tRNA-dependent wheat germ protein-synthesizing system View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1989-03

AUTHORS

H. Pfitzinger, J. H. Weil, D. T. N. Pillay, P. Guillemaut

ABSTRACT

A method is described for the preparation of a tRNA-dependent wheat germ protein-synthesizing system. This system can be supplied with exogenous tRNAs of eukaryotic or prokaryotic origin. In order to obtain maximal aminoacylation of the added tRNAs, the translation assays can be supplemented with homologous aminoacyl-tRNA synthetase preparations. Such a tRNA-dependent wheat germ protein-synthesizing system, which is easy to prepare, can be used not only to translate plant cytoplasmic mRNAs in the presence of added cytoplasmic tRNAs, but also to determine the translation activity of exogenous tRNAs from various sources in the presence of either natural or in vitro synthesized mRNAs. More... »

PAGES

301-306

References to SciGraph publications

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf00043207

DOI

http://dx.doi.org/10.1007/bf00043207

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1003027726

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/24272865


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