Details of T-DNA structural organization from a transgenic Petunia population exhibiting co-suppression View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1996-12

AUTHORS

Paul D. Cluster, Michael O'Dell, Michael Metzlaff, Richard B. Flavell

ABSTRACT

Analysis of Agrobacterium-transferred DNA (T-DNA) revealed strong correlations between transgene structures and floral pigmentation patterns from chalcone synthase (chs) co-suppression among 47 Petunia transformants. Presented here are the full details of T-DNA structural organization in that population. Sixteen transformants (34%) carried one T-DNA copy while 31 (66%) carried 106 complete and partial T-DNA elements in 54 linkage groups. Thirty linkage groups contained multiple T-DNA copies; 15 of these contained only contiguously repeated copies, 8 contained only dispersed copies and 7 contained both. Right-border inverted repeats were three times more frequent than left-border inverted or direct repeats. Large fragments of binary-vector sequences were linked to the T-DNA in seven plants. More... »

PAGES

1197-1203

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf00041406

DOI

http://dx.doi.org/10.1007/bf00041406

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1027472940

PUBMED

https://www.ncbi.nlm.nih.gov/pubmed/9002621


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