Conditions for high frequency embryogenesis from orange (Citrus sinensis Osb.) protoplasts View Full Text


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Article Info

DATE

1985-09

AUTHORS

Shozo Kobayashi, Isami Ikeda, Hirofumi Uchimiya

ABSTRACT

Using ‘Trovita’ orange (Citrus sinensis Osb.) protoplasts isolated from 6-year-old nucellar callus, the effects of protoplast density and mannitol concentration on cell divisions and embryoid formation were examined. Somatic embryogenesis in nearly direct manner was observed only at a combination of low cell densities (∼4×104/ml) and low mannitol concentrations (∼0.4 M). Two alternatives to achieve high frequency embryogenesis (∼70%) were to either dilute the cells to lower densities, or to do serial transfers of cells to fresh medium. Orange protoplasts (cells) showed embryogenic potential, and repression of embryogenesis occurred when protoplasts were cultured at a high density and/or under high osmotic pressure. More... »

PAGES

249-259

References to SciGraph publications

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf00040199

DOI

http://dx.doi.org/10.1007/bf00040199

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1030210341


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