Stimulation of in vitro shoot proliferation from nodal explants of cassava by thidiazuron, benzyladenine and gibberellic acid View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1996-07

AUTHORS

Basdeo Bhagwat, Luiz G. F. Vieiral, Larry R. Erickson

ABSTRACT

Multiple shoots were produced from nodal explants of cassava (Manihot esculenta Crantz) by a two-step procedure: a 6- to 8-day exposure to 0.11–0.22 µM thidiazuron (TDZ) in liquid Murashige and Skoog (MS) medium followed by culture on agar-solidified MS medium supplemented with 2.2 µM 6-benzyladenine (BA) and 1.6 μM gibberellic acid (GA3). TDZ caused the nodal explants to expand and this expansion (growth) continued during culture with BA and GA3. From this expanded explant, clusters of buds and fasciated stems developed continuously and these gave rise to shoots. The shoot proliferation process was open-ended, yielding an average of 31.5 shoots per nodal explant after 10 weeks of culture with genotype CG 1–56. A positive response was also obtained from seven other genotypes evaluated with this protocol. More... »

PAGES

1-7

References to SciGraph publications

  • 1993-05. Thidiazuron: a potent cytokinin for woody plant tissue culture in PLANT CELL, TISSUE AND ORGAN CULTURE (PCTOC)
  • 1993-05. Induction, germination and shoot development of somatic embryos in cassava in PLANT CELL, TISSUE AND ORGAN CULTURE (PCTOC)
  • 1989-01. Adventitions shoot formation on excised leaves of in vitro grown shoots of apple cultivars in PLANT CELL, TISSUE AND ORGAN CULTURE (PCTOC)
  • 1993-04. Improvement of somatic embryogenesis and plant recovery in cassava in PLANT CELL REPORTS
  • 1987-06. In vitro somatic embryogenesis and plant regeneration of cassava in PLANT CELL REPORTS
  • 1986-05. In vitro propagation of cassava (Manihot esculenta Crantz) in PLANT CELL, TISSUE AND ORGAN CULTURE (PCTOC)
  • 1992-10. Thidiazuron stimulates shoot organogenesis and somatic embryogenesis in white ash (Fraxinus americana L.) in PLANT CELL, TISSUE AND ORGAN CULTURE (PCTOC)
  • Identifiers

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    http://scigraph.springernature.com/pub.10.1007/bf00039690

    DOI

    http://dx.doi.org/10.1007/bf00039690

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1011619185


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