The effect of T-DNA copy number, position and methylation on reporter gene expression in tobacco transformants View Full Text


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Article Info

DATE

1990-12

AUTHORS

Shaun L. A. Hobbs, Pascal Kpodar, Catherine M. O. DeLong

ABSTRACT

Inter-transformant variability in the expression of introduced genes was studied in the R1 and R2 generations of 10 tobacco transformants, produced by Agrobacterium-mediated transformation. In replicated and physiologically equivalent material, tranformants showed considerable variability in the expression of the reporter gene uidA as shown by transcript levels and beta-glucuronidase (GUS) activity. However, homozygous R2 material could be investigated for seven of the transformants and among these, and in one line in which two inserts could segregate independently, this inter-transformant variability was reduced to simple bimodal expression. The two levels of expression for GUS activity in leaves were high or low (approximately 2.5 or 0.3 nmol cm-2 min-1 respectively), with no continuous variation. Transformants in the high group had single T-DNA insertions, while those in the low group had multiple T-DNA insertions, at the same or different loci. Within each group, although T-DNA was apparently integrated at different sites in the plant genome, there was no evidence of position effects. GUS activity levels of the transformants were very similar in the field and in environmentally controlled conditions under high or low light. Plants with multiple insertions and low expression also tended to have increased methylation of the integrated T-DNA. More... »

PAGES

851-864

References to SciGraph publications

  • 1987-12. Assaying chimeric genes in plants: The GUS gene fusion system in PLANT MOLECULAR BIOLOGY REPORTER
  • 1987-07. Expression of a soybean β-conclycinin gene under the control of the Cauliflower Mosaic Virus 35S and 19S promoters in transformed petunia tissues in PLANT MOLECULAR BIOLOGY
  • 1987-05. T-DNA is organized predominantly in inverted repeat structures in plants transformed with Agrobacterium tumefaciens C58 derivatives in MOLECULAR GENETICS AND GENOMICS
  • 1987-05. T-DNA structure and gene expression in petunia plants transformed by Agrobacterium tumefaciens C58 derivatives in MOLECULAR GENETICS AND GENOMICS
  • 1985-05. Molecular and general genetics of a hybrid foreign gene introduced into tobacco by direct gene transfer in MOLECULAR GENETICS AND GENOMICS
  • 1990-03. Genetic variability in the amount of ribulose-1,5-bisphosphate carboxylase/oxygenase and its small subunit mRNA in pea in PLANTA
  • 1986-10. Isolation and characterization of a light-inducible, organ-specific gene from potato and analysis of its expression after tagging and transfer into tobacco and potato shoots in MOLECULAR GENETICS AND GENOMICS
  • 1988-12. Genetic analysis of T-DNA insertions into the tobacco genome in PLANT CELL REPORTS
  • 1986-10. T-DNA structure in transgenic tobacco plants with multiple independent integration sites in MOLECULAR GENETICS AND GENOMICS
  • 1989-01. Evaluation of Herbicide Resistance in Transgenic Crops Under Field Conditions in NATURE BIOTECHNOLOGY
  • 1990-02. Synthesis and accumulation of pea plastocyanin in transgenic tobacco plants in PLANT MOLECULAR BIOLOGY
  • 1988-05. Analysis of the T-DNA structure in a large number of transgenic petunias generated by Agrobacterium-mediated transformation in PLANT MOLECULAR BIOLOGY
  • 1986-09. The promoter of TL-DNA gene 5 controls the tissue-specific expression of chimaeric genes carried by a novel type of Agrobacterium binary vector in MOLECULAR GENETICS AND GENOMICS
  • 1988-04. Virus Tolerance, Plant Performance of Transgenic Tomato Plants Expressing Coat Protein from Tobacco Mosaic Virus in NATURE BIOTECHNOLOGY
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/bf00039425

    DOI

    http://dx.doi.org/10.1007/bf00039425

    DIMENSIONS

    https://app.dimensions.ai/details/publication/pub.1029834259

    PUBMED

    https://www.ncbi.nlm.nih.gov/pubmed/2103477


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