Micropropagation of kiwifruit using non-axenic shoot tips View Full Text


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Article Info

DATE

1986-01

AUTHORS

Paul L. Monette

ABSTRACT

Kiwifruit (Actinidia chinensis Planch.) shoot tips were subjected to a standard surface sterilization procedure and cultured on a Murashige and Skoog basal medium in the presence of two surviving bacterial contaminants. The fresh weight increase of the cultures and the number of shoots produced were greater in liquid medium than in medium solidified with 0.4 or 0.8% agar. A greater number of shoots was obtained with 125 ml than with 50, 250, or 500 ml Erlenmeyer flasks. A concentration of 2 mgl-1 N6-benzylaminopurine (BAP) gave a greater increase in fresh weight than either 0 or 4 mgl-1. Shoots cut from proliferating cultures were dipped in 0.05% indolebutyric acid (IBA) and rooted directly in a peat: vermiculite: perlite mix. Over 93 % of 907 plantlets produced were successfully acclimatized. The productivity of the method was comparable to that reported for the axenic culture of meristems. The contaminants which survived the initial surface sterilization procedure thus presented no major obstacle to the in vitro propagation of kiwifruit. More... »

PAGES

73-82

References to SciGraph publications

  • 1983-12. Influence of size of culture vessel on in vitro proliferation of grape in a liquid medium in PLANT CELL, TISSUE AND ORGAN CULTURE (PCTOC)
  • 1982-03. Factors influencing the production of hardened glaucous carnation plantlets in vitro in PLANT CELL, TISSUE AND ORGAN CULTURE (PCTOC)
  • Identifiers

    URI

    http://scigraph.springernature.com/pub.10.1007/bf00037760

    DOI

    http://dx.doi.org/10.1007/bf00037760

    DIMENSIONS

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