Factors effecting adventitious shoot regeneration from leaf explants of quince (Cydonia oblonga) View Full Text


Ontology type: schema:ScholarlyArticle     


Article Info

DATE

1993-12

AUTHORS

Barton S. Baker, Satish K. Bhatia

ABSTRACT

A procedure for adventitious shoot regeneration from leaf explants of quince (Cydonia oblonga Mill.) using thidiazuron (TDZ) was developed. Excised leaves of cultures grown on Murashige and Skoog (MS) medium containing 5 μM benzyladenine (BA) and 0.9% Gibco Phytagar were used. Several experiments were conducted to determine optimum concentrations of thidiazuron, α-naphthaleneacetic acid (NAA) and sucrose. When the medium contained 1.5 μM TDZ and 2.5 μM NAA, 85% of the discs regenerated shoots with an average of eight shoots per leaf disc. An incubation period of three weeks in the dark was necessary for optimum shoot regeneration. Leaves excised from four to six-week-old cultures gave a higher percent shoot regeneration than leaves from cultures older than six weeks. Regeneration percentages were significantly reduced when sucrose concentration in the medium was less than 3%. A significantly higher percentage of shoots regenerated when leaf discs were placed on the regeneration medium abaxial side down as compared to the adaxial side. Regenerated shoots were cultured on MS medium containing 5 μM BA and rooted on half-strength MS medium containing 10 μM NAA. Rooted plantlets were acclimatized to greenhouse conditions for evaluation of any somaclonal variation. The importance of these findings are discussed in relation to in vitro improvement of plants. More... »

PAGES

273-277

References to SciGraph publications

Identifiers

URI

http://scigraph.springernature.com/pub.10.1007/bf00037281

DOI

http://dx.doi.org/10.1007/bf00037281

DIMENSIONS

https://app.dimensions.ai/details/publication/pub.1012216469


Indexing Status Check whether this publication has been indexed by Scopus and Web Of Science using the SN Indexing Status Tool
Incoming Citations Browse incoming citations for this publication using opencitations.net

JSON-LD is the canonical representation for SciGraph data.

TIP: You can open this SciGraph record using an external JSON-LD service: JSON-LD Playground Google SDTT

[
  {
    "@context": "https://springernature.github.io/scigraph/jsonld/sgcontext.json", 
    "about": [
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/0607", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Plant Biology", 
        "type": "DefinedTerm"
      }, 
      {
        "id": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/06", 
        "inDefinedTermSet": "http://purl.org/au-research/vocabulary/anzsrc-for/2008/", 
        "name": "Biological Sciences", 
        "type": "DefinedTerm"
      }
    ], 
    "author": [
      {
        "affiliation": {
          "alternateName": "West Virginia University", 
          "id": "https://www.grid.ac/institutes/grid.268154.c", 
          "name": [
            "Division of Plant and Soil Sciences, West Virginia University, 26506-6108, Morgantown, West Virginia, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Baker", 
        "givenName": "Barton S.", 
        "id": "sg:person.010406105525.97", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.010406105525.97"
        ], 
        "type": "Person"
      }, 
      {
        "affiliation": {
          "alternateName": "West Virginia University", 
          "id": "https://www.grid.ac/institutes/grid.268154.c", 
          "name": [
            "Division of Plant and Soil Sciences, West Virginia University, 26506-6108, Morgantown, West Virginia, USA"
          ], 
          "type": "Organization"
        }, 
        "familyName": "Bhatia", 
        "givenName": "Satish K.", 
        "id": "sg:person.01242311255.46", 
        "sameAs": [
          "https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01242311255.46"
        ], 
        "type": "Person"
      }
    ], 
    "citation": [
      {
        "id": "sg:pub.10.1007/bf00047611", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1007107574", 
          "https://doi.org/10.1007/bf00047611"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00047611", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1007107574", 
          "https://doi.org/10.1007/bf00047611"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00036516", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1022981893", 
          "https://doi.org/10.1007/bf00036516"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00036516", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1022981893", 
          "https://doi.org/10.1007/bf00036516"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1111/j.1399-3054.1962.tb08052.x", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1023703429"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0176-1617(88)80152-4", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1024841153"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00033906", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1030591619", 
          "https://doi.org/10.1007/bf00033906"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00033906", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1030591619", 
          "https://doi.org/10.1007/bf00033906"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00043688", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1038338054", 
          "https://doi.org/10.1007/bf00043688"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00043688", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1038338054", 
          "https://doi.org/10.1007/bf00043688"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/s0031-9422(82)85007-3", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1038778184"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00035834", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1040529887", 
          "https://doi.org/10.1007/bf00035834"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00035834", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1040529887", 
          "https://doi.org/10.1007/bf00035834"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00034490", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1042656962", 
          "https://doi.org/10.1007/bf00034490"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00034490", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1042656962", 
          "https://doi.org/10.1007/bf00034490"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00232566", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1042743526", 
          "https://doi.org/10.1007/bf00232566"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00232566", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1042743526", 
          "https://doi.org/10.1007/bf00232566"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0304-4238(86)90105-6", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1052276916"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "https://doi.org/10.1016/0304-4238(86)90105-6", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1052276916"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00272062", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1078814085", 
          "https://doi.org/10.1007/bf00272062"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00041885", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1086068896", 
          "https://doi.org/10.1007/bf00041885"
        ], 
        "type": "CreativeWork"
      }, 
      {
        "id": "sg:pub.10.1007/bf00042180", 
        "sameAs": [
          "https://app.dimensions.ai/details/publication/pub.1086069539", 
          "https://doi.org/10.1007/bf00042180"
        ], 
        "type": "CreativeWork"
      }
    ], 
    "datePublished": "1993-12", 
    "datePublishedReg": "1993-12-01", 
    "description": "A procedure for adventitious shoot regeneration from leaf explants of quince (Cydonia oblonga Mill.) using thidiazuron (TDZ) was developed. Excised leaves of cultures grown on Murashige and Skoog (MS) medium containing 5 \u03bcM benzyladenine (BA) and 0.9% Gibco Phytagar were used. Several experiments were conducted to determine optimum concentrations of thidiazuron, \u03b1-naphthaleneacetic acid (NAA) and sucrose. When the medium contained 1.5 \u03bcM TDZ and 2.5 \u03bcM NAA, 85% of the discs regenerated shoots with an average of eight shoots per leaf disc. An incubation period of three weeks in the dark was necessary for optimum shoot regeneration. Leaves excised from four to six-week-old cultures gave a higher percent shoot regeneration than leaves from cultures older than six weeks. Regeneration percentages were significantly reduced when sucrose concentration in the medium was less than 3%. A significantly higher percentage of shoots regenerated when leaf discs were placed on the regeneration medium abaxial side down as compared to the adaxial side. Regenerated shoots were cultured on MS medium containing 5 \u03bcM BA and rooted on half-strength MS medium containing 10 \u03bcM NAA. Rooted plantlets were acclimatized to greenhouse conditions for evaluation of any somaclonal variation. The importance of these findings are discussed in relation to in vitro improvement of plants.", 
    "genre": "research_article", 
    "id": "sg:pub.10.1007/bf00037281", 
    "inLanguage": [
      "en"
    ], 
    "isAccessibleForFree": false, 
    "isPartOf": [
      {
        "id": "sg:journal.1123614", 
        "issn": [
          "0167-6857", 
          "1573-5044"
        ], 
        "name": "Plant Cell, Tissue and Organ Culture (PCTOC)", 
        "type": "Periodical"
      }, 
      {
        "issueNumber": "3", 
        "type": "PublicationIssue"
      }, 
      {
        "type": "PublicationVolume", 
        "volumeNumber": "35"
      }
    ], 
    "name": "Factors effecting adventitious shoot regeneration from leaf explants of quince (Cydonia oblonga)", 
    "pagination": "273-277", 
    "productId": [
      {
        "name": "readcube_id", 
        "type": "PropertyValue", 
        "value": [
          "3a340bb3416b52066a9c4dd9c62d929bd8a80e396d5a4bbdb4edb99edb008fcb"
        ]
      }, 
      {
        "name": "doi", 
        "type": "PropertyValue", 
        "value": [
          "10.1007/bf00037281"
        ]
      }, 
      {
        "name": "dimensions_id", 
        "type": "PropertyValue", 
        "value": [
          "pub.1012216469"
        ]
      }
    ], 
    "sameAs": [
      "https://doi.org/10.1007/bf00037281", 
      "https://app.dimensions.ai/details/publication/pub.1012216469"
    ], 
    "sdDataset": "articles", 
    "sdDatePublished": "2019-04-11T13:55", 
    "sdLicense": "https://scigraph.springernature.com/explorer/license/", 
    "sdPublisher": {
      "name": "Springer Nature - SN SciGraph project", 
      "type": "Organization"
    }, 
    "sdSource": "s3://com-uberresearch-data-dimensions-target-20181106-alternative/cleanup/v134/2549eaecd7973599484d7c17b260dba0a4ecb94b/merge/v9/a6c9fde33151104705d4d7ff012ea9563521a3ce/jats-lookup/v90/0000000371_0000000371/records_130814_00000001.jsonl", 
    "type": "ScholarlyArticle", 
    "url": "http://link.springer.com/10.1007/BF00037281"
  }
]
 

Download the RDF metadata as:  json-ld nt turtle xml License info

HOW TO GET THIS DATA PROGRAMMATICALLY:

JSON-LD is a popular format for linked data which is fully compatible with JSON.

curl -H 'Accept: application/ld+json' 'https://scigraph.springernature.com/pub.10.1007/bf00037281'

N-Triples is a line-based linked data format ideal for batch operations.

curl -H 'Accept: application/n-triples' 'https://scigraph.springernature.com/pub.10.1007/bf00037281'

Turtle is a human-readable linked data format.

curl -H 'Accept: text/turtle' 'https://scigraph.springernature.com/pub.10.1007/bf00037281'

RDF/XML is a standard XML format for linked data.

curl -H 'Accept: application/rdf+xml' 'https://scigraph.springernature.com/pub.10.1007/bf00037281'


 

This table displays all metadata directly associated to this object as RDF triples.

120 TRIPLES      21 PREDICATES      41 URIs      19 LITERALS      7 BLANK NODES

Subject Predicate Object
1 sg:pub.10.1007/bf00037281 schema:about anzsrc-for:06
2 anzsrc-for:0607
3 schema:author N8dd3641a9832407f993e380a83e127ff
4 schema:citation sg:pub.10.1007/bf00033906
5 sg:pub.10.1007/bf00034490
6 sg:pub.10.1007/bf00035834
7 sg:pub.10.1007/bf00036516
8 sg:pub.10.1007/bf00041885
9 sg:pub.10.1007/bf00042180
10 sg:pub.10.1007/bf00043688
11 sg:pub.10.1007/bf00047611
12 sg:pub.10.1007/bf00232566
13 sg:pub.10.1007/bf00272062
14 https://doi.org/10.1016/0304-4238(86)90105-6
15 https://doi.org/10.1016/s0031-9422(82)85007-3
16 https://doi.org/10.1016/s0176-1617(88)80152-4
17 https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
18 schema:datePublished 1993-12
19 schema:datePublishedReg 1993-12-01
20 schema:description A procedure for adventitious shoot regeneration from leaf explants of quince (Cydonia oblonga Mill.) using thidiazuron (TDZ) was developed. Excised leaves of cultures grown on Murashige and Skoog (MS) medium containing 5 μM benzyladenine (BA) and 0.9% Gibco Phytagar were used. Several experiments were conducted to determine optimum concentrations of thidiazuron, α-naphthaleneacetic acid (NAA) and sucrose. When the medium contained 1.5 μM TDZ and 2.5 μM NAA, 85% of the discs regenerated shoots with an average of eight shoots per leaf disc. An incubation period of three weeks in the dark was necessary for optimum shoot regeneration. Leaves excised from four to six-week-old cultures gave a higher percent shoot regeneration than leaves from cultures older than six weeks. Regeneration percentages were significantly reduced when sucrose concentration in the medium was less than 3%. A significantly higher percentage of shoots regenerated when leaf discs were placed on the regeneration medium abaxial side down as compared to the adaxial side. Regenerated shoots were cultured on MS medium containing 5 μM BA and rooted on half-strength MS medium containing 10 μM NAA. Rooted plantlets were acclimatized to greenhouse conditions for evaluation of any somaclonal variation. The importance of these findings are discussed in relation to in vitro improvement of plants.
21 schema:genre research_article
22 schema:inLanguage en
23 schema:isAccessibleForFree false
24 schema:isPartOf N0897b3519dc44a1186aff01a21fe4951
25 N6be65eab96c140c4b9e0291c90f88539
26 sg:journal.1123614
27 schema:name Factors effecting adventitious shoot regeneration from leaf explants of quince (Cydonia oblonga)
28 schema:pagination 273-277
29 schema:productId N31ea3c581e1940dbbea5af00d46447ca
30 Nabc21771382344a7b0836ff03678e153
31 Nef8a0c3728964e10a8761b70daca3d16
32 schema:sameAs https://app.dimensions.ai/details/publication/pub.1012216469
33 https://doi.org/10.1007/bf00037281
34 schema:sdDatePublished 2019-04-11T13:55
35 schema:sdLicense https://scigraph.springernature.com/explorer/license/
36 schema:sdPublisher Nba120236ba224a75a9f9ae6afcc8ae6f
37 schema:url http://link.springer.com/10.1007/BF00037281
38 sgo:license sg:explorer/license/
39 sgo:sdDataset articles
40 rdf:type schema:ScholarlyArticle
41 N0897b3519dc44a1186aff01a21fe4951 schema:volumeNumber 35
42 rdf:type schema:PublicationVolume
43 N31ea3c581e1940dbbea5af00d46447ca schema:name doi
44 schema:value 10.1007/bf00037281
45 rdf:type schema:PropertyValue
46 N38ef312abfd84660baf95b61572841b2 rdf:first sg:person.01242311255.46
47 rdf:rest rdf:nil
48 N6be65eab96c140c4b9e0291c90f88539 schema:issueNumber 3
49 rdf:type schema:PublicationIssue
50 N8dd3641a9832407f993e380a83e127ff rdf:first sg:person.010406105525.97
51 rdf:rest N38ef312abfd84660baf95b61572841b2
52 Nabc21771382344a7b0836ff03678e153 schema:name dimensions_id
53 schema:value pub.1012216469
54 rdf:type schema:PropertyValue
55 Nba120236ba224a75a9f9ae6afcc8ae6f schema:name Springer Nature - SN SciGraph project
56 rdf:type schema:Organization
57 Nef8a0c3728964e10a8761b70daca3d16 schema:name readcube_id
58 schema:value 3a340bb3416b52066a9c4dd9c62d929bd8a80e396d5a4bbdb4edb99edb008fcb
59 rdf:type schema:PropertyValue
60 anzsrc-for:06 schema:inDefinedTermSet anzsrc-for:
61 schema:name Biological Sciences
62 rdf:type schema:DefinedTerm
63 anzsrc-for:0607 schema:inDefinedTermSet anzsrc-for:
64 schema:name Plant Biology
65 rdf:type schema:DefinedTerm
66 sg:journal.1123614 schema:issn 0167-6857
67 1573-5044
68 schema:name Plant Cell, Tissue and Organ Culture (PCTOC)
69 rdf:type schema:Periodical
70 sg:person.010406105525.97 schema:affiliation https://www.grid.ac/institutes/grid.268154.c
71 schema:familyName Baker
72 schema:givenName Barton S.
73 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.010406105525.97
74 rdf:type schema:Person
75 sg:person.01242311255.46 schema:affiliation https://www.grid.ac/institutes/grid.268154.c
76 schema:familyName Bhatia
77 schema:givenName Satish K.
78 schema:sameAs https://app.dimensions.ai/discover/publication?and_facet_researcher=ur.01242311255.46
79 rdf:type schema:Person
80 sg:pub.10.1007/bf00033906 schema:sameAs https://app.dimensions.ai/details/publication/pub.1030591619
81 https://doi.org/10.1007/bf00033906
82 rdf:type schema:CreativeWork
83 sg:pub.10.1007/bf00034490 schema:sameAs https://app.dimensions.ai/details/publication/pub.1042656962
84 https://doi.org/10.1007/bf00034490
85 rdf:type schema:CreativeWork
86 sg:pub.10.1007/bf00035834 schema:sameAs https://app.dimensions.ai/details/publication/pub.1040529887
87 https://doi.org/10.1007/bf00035834
88 rdf:type schema:CreativeWork
89 sg:pub.10.1007/bf00036516 schema:sameAs https://app.dimensions.ai/details/publication/pub.1022981893
90 https://doi.org/10.1007/bf00036516
91 rdf:type schema:CreativeWork
92 sg:pub.10.1007/bf00041885 schema:sameAs https://app.dimensions.ai/details/publication/pub.1086068896
93 https://doi.org/10.1007/bf00041885
94 rdf:type schema:CreativeWork
95 sg:pub.10.1007/bf00042180 schema:sameAs https://app.dimensions.ai/details/publication/pub.1086069539
96 https://doi.org/10.1007/bf00042180
97 rdf:type schema:CreativeWork
98 sg:pub.10.1007/bf00043688 schema:sameAs https://app.dimensions.ai/details/publication/pub.1038338054
99 https://doi.org/10.1007/bf00043688
100 rdf:type schema:CreativeWork
101 sg:pub.10.1007/bf00047611 schema:sameAs https://app.dimensions.ai/details/publication/pub.1007107574
102 https://doi.org/10.1007/bf00047611
103 rdf:type schema:CreativeWork
104 sg:pub.10.1007/bf00232566 schema:sameAs https://app.dimensions.ai/details/publication/pub.1042743526
105 https://doi.org/10.1007/bf00232566
106 rdf:type schema:CreativeWork
107 sg:pub.10.1007/bf00272062 schema:sameAs https://app.dimensions.ai/details/publication/pub.1078814085
108 https://doi.org/10.1007/bf00272062
109 rdf:type schema:CreativeWork
110 https://doi.org/10.1016/0304-4238(86)90105-6 schema:sameAs https://app.dimensions.ai/details/publication/pub.1052276916
111 rdf:type schema:CreativeWork
112 https://doi.org/10.1016/s0031-9422(82)85007-3 schema:sameAs https://app.dimensions.ai/details/publication/pub.1038778184
113 rdf:type schema:CreativeWork
114 https://doi.org/10.1016/s0176-1617(88)80152-4 schema:sameAs https://app.dimensions.ai/details/publication/pub.1024841153
115 rdf:type schema:CreativeWork
116 https://doi.org/10.1111/j.1399-3054.1962.tb08052.x schema:sameAs https://app.dimensions.ai/details/publication/pub.1023703429
117 rdf:type schema:CreativeWork
118 https://www.grid.ac/institutes/grid.268154.c schema:alternateName West Virginia University
119 schema:name Division of Plant and Soil Sciences, West Virginia University, 26506-6108, Morgantown, West Virginia, USA
120 rdf:type schema:Organization
 




Preview window. Press ESC to close (or click here)


...